Click here to switch to the map view.

The map label for this gene is acoA [H]

Identifier: 222527177

GI number: 222527177

Start: 4908001

End: 4909017

Strand: Reverse

Name: acoA [H]

Synonym: Chy400_3959

Alternate gene names: 222527177

Gene position: 4909017-4908001 (Counterclockwise)

Preceding gene: 222527181

Following gene: 222527176

Centisome position: 93.17

GC content: 57.92

Gene sequence:

>1017_bases
ATGGACATCAGTCGGGAGAAACTCCTCTGGGCGTATGAGCGGATGCGGCTCATTCGTGAGTTCGAGGATCGGTTGCACAC
CGATTTTGCTGCCGGGAAGATACCTGGTTTCGTTCACCTGTATGCCGGTGAAGAAGCGGTAGCTGTTGGTCTCTGCGCGC
ACCTGCGGGATGATGATTTTATTACCAGCACCCATCGCGGTCACGGGCACTGCATTGCCAAGGGAGTCGATCTGCGGGCG
ATGATGGCCGAGATCTACGGCAAGGCAACAGGAGCCTGTAAGGGCAAAGGCGGTTCGATGCATATTGCCGATGTCGATAA
GGGCATGCTTGGCGCCAACGGCATTGTTGGTGGCGGCCCGCCACTGGCCTGTGGTGCCGGTCTGACCGCGAAGATCAAGG
GTACCGATCAGGTAACGGTTTGCTTCTTCGGTGATGGAGCGTCGAATCAGGGTACGACGTTTGAGGGTCTGAATCTGGCG
GGAATCTGGAAATTGCCGGTCGTGTTTGTGTGTGAGAATAACGGCTACGCCGAGACGACGTCACCCCGTTATTCTGTTTC
CGGGCAAGACATCGCTGCACGCGCACGTGGGTTTGGTATGCCGAGCATTGCCATTGACGGCCTCGATTTCTTTGCCGTTT
ACGAGGCAGCCGGTGAAGCTGTAGCGCGTGCCCGGCGCGGGGAAGGCCCGACGTTTATCGAGGCGCAGACGTATCGCTAC
TATGGTCACTTCGAGGGTGACTCGATCCGCTATCGCACTCGCGATGAGGAAGCTCATTATCGCTCGCTCGACTGTCTCCA
CCGTTTTCGCCAGACTGTGACAGCGCAGGGATTGTTGACGGCTGCTGAGCTTGATGAGATCGATGCCCGTGCTCGTGCCG
CGGTGGAAGATGCGGTACGCTTCGCTGCCGAAAGCCCATTGCCCGATCCGGCAGAGCTGTTGACCGATGTCTATGTTGAT
TATCCGATGTCGGCTTTGTGGCCCTTCCAGGAAGCTGCCGTCGCAGTACGACGCTAG

Upstream 100 bases:

>100_bases
CATCTCAATCGGGGTTCTGCGCATCCCAGTCTGATTTTCCTTTCCGGCTCGCTGGCGAGCTATGGCGATAGTTGGCGTTC
ATTGACGAACGGAGAGTGCT

Downstream 100 bases:

>100_bases
ATGCGAATAGAGATAGTGAGCTGTAGGGAAGGGGTAGCTTTCCTTCGGCACAGACAACTCAACGATGAGGTGATACTATG
ACTGCAATGGTTGAAGAGGC

Product: pyruvate dehydrogenase

Products: [dihydrolipoyllysine-residue acetyltransferase] ; $S-acetyldihydrolipoyllysine; CO2

Alternate protein names: Acetoin:DCPIP oxidoreductase-alpha; Ao:DCPIP OR; TPP-dependent acetoin dehydrogenase E1 subunit alpha [H]

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MDISREKLLWAYERMRLIREFEDRLHTDFAAGKIPGFVHLYAGEEAVAVGLCAHLRDDDFITSTHRGHGHCIAKGVDLRA
MMAEIYGKATGACKGKGGSMHIADVDKGMLGANGIVGGGPPLACGAGLTAKIKGTDQVTVCFFGDGASNQGTTFEGLNLA
GIWKLPVVFVCENNGYAETTSPRYSVSGQDIAARARGFGMPSIAIDGLDFFAVYEAAGEAVARARRGEGPTFIEAQTYRY
YGHFEGDSIRYRTRDEEAHYRSLDCLHRFRQTVTAQGLLTAAELDEIDARARAAVEDAVRFAAESPLPDPAELLTDVYVD
YPMSALWPFQEAAVAVRR

Sequences:

>Translated_338_residues
MDISREKLLWAYERMRLIREFEDRLHTDFAAGKIPGFVHLYAGEEAVAVGLCAHLRDDDFITSTHRGHGHCIAKGVDLRA
MMAEIYGKATGACKGKGGSMHIADVDKGMLGANGIVGGGPPLACGAGLTAKIKGTDQVTVCFFGDGASNQGTTFEGLNLA
GIWKLPVVFVCENNGYAETTSPRYSVSGQDIAARARGFGMPSIAIDGLDFFAVYEAAGEAVARARRGEGPTFIEAQTYRY
YGHFEGDSIRYRTRDEEAHYRSLDCLHRFRQTVTAQGLLTAAELDEIDARARAAVEDAVRFAAESPLPDPAELLTDVYVD
YPMSALWPFQEAAVAVRR
>Mature_338_residues
MDISREKLLWAYERMRLIREFEDRLHTDFAAGKIPGFVHLYAGEEAVAVGLCAHLRDDDFITSTHRGHGHCIAKGVDLRA
MMAEIYGKATGACKGKGGSMHIADVDKGMLGANGIVGGGPPLACGAGLTAKIKGTDQVTVCFFGDGASNQGTTFEGLNLA
GIWKLPVVFVCENNGYAETTSPRYSVSGQDIAARARGFGMPSIAIDGLDFFAVYEAAGEAVARARRGEGPTFIEAQTYRY
YGHFEGDSIRYRTRDEEAHYRSLDCLHRFRQTVTAQGLLTAAELDEIDARARAAVEDAVRFAAESPLPDPAELLTDVYVD
YPMSALWPFQEAAVAVRR

Specific function: Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage of acetoin into acetate and acetaldehyde. The alpha subunit is probably the catalytic subunit of the enzyme [H]

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4885543, Length=318, Percent_Identity=38.9937106918239, Blast_Score=222, Evalue=5e-58,
Organism=Homo sapiens, GI291084742, Length=330, Percent_Identity=38.7878787878788, Blast_Score=208, Evalue=4e-54,
Organism=Homo sapiens, GI4505685, Length=330, Percent_Identity=38.7878787878788, Blast_Score=208, Evalue=6e-54,
Organism=Homo sapiens, GI291084744, Length=337, Percent_Identity=37.9821958456973, Blast_Score=201, Evalue=6e-52,
Organism=Homo sapiens, GI291084757, Length=330, Percent_Identity=34.8484848484849, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI11386135, Length=322, Percent_Identity=32.6086956521739, Blast_Score=162, Evalue=6e-40,
Organism=Homo sapiens, GI258645172, Length=322, Percent_Identity=32.2981366459627, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI32564172, Length=323, Percent_Identity=38.6996904024768, Blast_Score=215, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI17536047, Length=323, Percent_Identity=38.6996904024768, Blast_Score=215, Evalue=3e-56,
Organism=Caenorhabditis elegans, GI86563355, Length=320, Percent_Identity=29.375, Blast_Score=127, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI86563357, Length=320, Percent_Identity=29.375, Blast_Score=127, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6321026, Length=322, Percent_Identity=38.5093167701863, Blast_Score=209, Evalue=4e-55,
Organism=Drosophila melanogaster, GI24639744, Length=322, Percent_Identity=38.8198757763975, Blast_Score=211, Evalue=5e-55,
Organism=Drosophila melanogaster, GI28571106, Length=322, Percent_Identity=38.8198757763975, Blast_Score=211, Evalue=5e-55,
Organism=Drosophila melanogaster, GI24639740, Length=322, Percent_Identity=38.8198757763975, Blast_Score=211, Evalue=7e-55,
Organism=Drosophila melanogaster, GI24639746, Length=308, Percent_Identity=39.9350649350649, Blast_Score=205, Evalue=4e-53,
Organism=Drosophila melanogaster, GI24639748, Length=320, Percent_Identity=38.4375, Blast_Score=196, Evalue=2e-50,
Organism=Drosophila melanogaster, GI21355903, Length=324, Percent_Identity=25.9259259259259, Blast_Score=112, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: 1.2.4.1

Molecular weight: Translated: 36713; Mature: 36713

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDISREKLLWAYERMRLIREFEDRLHTDFAAGKIPGFVHLYAGEEAVAVGLCAHLRDDDF
CCCCHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCEEEEEECCCHHHHHHHHHCCCCCC
ITSTHRGHGHCIAKGVDLRAMMAEIYGKATGACKGKGGSMHIADVDKGMLGANGIVGGGP
EECCCCCCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCC
PLACGAGLTAKIKGTDQVTVCFFGDGASNQGTTFEGLNLAGIWKLPVVFVCENNGYAETT
CCEECCCCEEEEECCCEEEEEEEECCCCCCCCEECCCCEEEEEECCEEEEECCCCCCCCC
SPRYSVSGQDIAARARGFGMPSIAIDGLDFFAVYEAAGEAVARARRGEGPTFIEAQTYRY
CCCEEECCCHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEE
YGHFEGDSIRYRTRDEEAHYRSLDCLHRFRQTVTAQGLLTAAELDEIDARARAAVEDAVR
EEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FAAESPLPDPAELLTDVYVDYPMSALWPFQEAAVAVRR
HHHCCCCCCHHHHHHHHHHCCCHHHHCCHHHHHHHHCC
>Mature Secondary Structure
MDISREKLLWAYERMRLIREFEDRLHTDFAAGKIPGFVHLYAGEEAVAVGLCAHLRDDDF
CCCCHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCEEEEEECCCHHHHHHHHHCCCCCC
ITSTHRGHGHCIAKGVDLRAMMAEIYGKATGACKGKGGSMHIADVDKGMLGANGIVGGGP
EECCCCCCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCC
PLACGAGLTAKIKGTDQVTVCFFGDGASNQGTTFEGLNLAGIWKLPVVFVCENNGYAETT
CCEECCCCEEEEECCCEEEEEEEECCCCCCCCEECCCCEEEEEECCEEEEECCCCCCCCC
SPRYSVSGQDIAARARGFGMPSIAIDGLDFFAVYEAAGEAVARARRGEGPTFIEAQTYRY
CCCEEECCCHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEE
YGHFEGDSIRYRTRDEEAHYRSLDCLHRFRQTVTAQGLLTAAELDEIDARARAAVEDAVR
EEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FAAESPLPDPAELLTDVYVDYPMSALWPFQEAAVAVRR
HHHCCCCCCHHHHHHHHHHCCCHHHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine

Specific reaction: pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S- $acetyldihydrolipoyllysine + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 10368162; 9384377 [H]