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The map label for this gene is liaR [H]

Identifier: 222526715

GI number: 222526715

Start: 4346160

End: 4346816

Strand: Direct

Name: liaR [H]

Synonym: Chy400_3486

Alternate gene names: 222526715

Gene position: 4346160-4346816 (Clockwise)

Preceding gene: 222526714

Following gene: 222526716

Centisome position: 82.49

GC content: 56.01

Gene sequence:

>657_bases
ATGGAACAGATTACGGTGCTGCTCATTGATGACCATCGCGTCGTGCGCCAGGGGTTGCGCGATTTTCTCGAATTACAACC
CGATATCGAGGTAGTCGGAGAAGCGGGTAGTGGTGCCGAAGGTGTGGAACTGGCCCGTGAATTGCTGCCCGATGTGGTCT
TGATGGATCTCGTCATGCCCGGTATTGATGGGGTCGAAACCACACGCCGCTTGAAAGCGGTTAGCCCCTCGTCACAGGTA
ATTGTCCTGACCAGTTTTGCCGACGACGACAAGGTCTTTCCTGCTATCAAAGCCGGCGCAATCTCATACCTGCTCAAAGA
CATCTCGCCCGAAGATCTGGCCCACGCGATCCGGGCGGCGTGTCGCGGTGAAGCCGTTCTTCATCCCGATGTGGCGGCCA
AATTGATGCAGGAGTTCAATACGCCGCGCCCAAACGAAGCACCGGTCGAGCAGCTCACCCCGCGTGAGATGGATGTGCTG
CGGCTGGTCGCGAAGGGTATGTCGAACAAGGAGATCGCCGAGACATTGATCGTCTCGGAAAAGACGGTCAAAACCCATAT
CAGCAATATTCTTTCAAAGCTACACCTGGCCGACCGTACCCAGGTAGCGATCTACGCCCTCCGCAAGCGGCTTGTCCCAA
TCGATGAGGAAGAATAA

Upstream 100 bases:

>100_bases
AATCTTGAACGACCTGAGTAACATGAGATGCGAGACAGGTAATACCAGTTGCTTGACATAATCAGCGACGTGGGAAGGGT
AGCAAGAAGGGGGTAGGTGG

Downstream 100 bases:

>100_bases
TGACAGCACGGGTTGTATTACTGGGTACCGGAACAGGTTTGCCCGATCCTGATCGAGCGTATACGCATCTGGTCTGGGAT
GGGCCGGGTGGGCCATTCCT

Product: LuxR family two component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MEQITVLLIDDHRVVRQGLRDFLELQPDIEVVGEAGSGAEGVELARELLPDVVLMDLVMPGIDGVETTRRLKAVSPSSQV
IVLTSFADDDKVFPAIKAGAISYLLKDISPEDLAHAIRAACRGEAVLHPDVAAKLMQEFNTPRPNEAPVEQLTPREMDVL
RLVAKGMSNKEIAETLIVSEKTVKTHISNILSKLHLADRTQVAIYALRKRLVPIDEEE

Sequences:

>Translated_218_residues
MEQITVLLIDDHRVVRQGLRDFLELQPDIEVVGEAGSGAEGVELARELLPDVVLMDLVMPGIDGVETTRRLKAVSPSSQV
IVLTSFADDDKVFPAIKAGAISYLLKDISPEDLAHAIRAACRGEAVLHPDVAAKLMQEFNTPRPNEAPVEQLTPREMDVL
RLVAKGMSNKEIAETLIVSEKTVKTHISNILSKLHLADRTQVAIYALRKRLVPIDEEE
>Mature_218_residues
MEQITVLLIDDHRVVRQGLRDFLELQPDIEVVGEAGSGAEGVELARELLPDVVLMDLVMPGIDGVETTRRLKAVSPSSQV
IVLTSFADDDKVFPAIKAGAISYLLKDISPEDLAHAIRAACRGEAVLHPDVAAKLMQEFNTPRPNEAPVEQLTPREMDVL
RLVAKGMSNKEIAETLIVSEKTVKTHISNILSKLHLADRTQVAIYALRKRLVPIDEEE

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788521, Length=205, Percent_Identity=43.9024390243902, Blast_Score=158, Evalue=3e-40,
Organism=Escherichia coli, GI1787473, Length=214, Percent_Identity=43.4579439252336, Blast_Score=147, Evalue=7e-37,
Organism=Escherichia coli, GI1788222, Length=209, Percent_Identity=35.4066985645933, Blast_Score=134, Evalue=5e-33,
Organism=Escherichia coli, GI1790102, Length=201, Percent_Identity=36.81592039801, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1786747, Length=206, Percent_Identity=29.126213592233, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788712, Length=208, Percent_Identity=25.4807692307692, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788546, Length=225, Percent_Identity=28.8888888888889, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI87082052, Length=105, Percent_Identity=39.0476190476191, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1787277, Length=114, Percent_Identity=32.4561403508772, Blast_Score=67, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 24000; Mature: 24000

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQITVLLIDDHRVVRQGLRDFLELQPDIEVVGEAGSGAEGVELARELLPDVVLMDLVMP
CCCEEEEEECCCHHHHHHHHHHHHCCCCHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHC
GIDGVETTRRLKAVSPSSQVIVLTSFADDDKVFPAIKAGAISYLLKDISPEDLAHAIRAA
CCCCHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
CRGEAVLHPDVAAKLMQEFNTPRPNEAPVEQLTPREMDVLRLVAKGMSNKEIAETLIVSE
HCCCCEECHHHHHHHHHHCCCCCCCCCCHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHH
KTVKTHISNILSKLHLADRTQVAIYALRKRLVPIDEEE
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MEQITVLLIDDHRVVRQGLRDFLELQPDIEVVGEAGSGAEGVELARELLPDVVLMDLVMP
CCCEEEEEECCCHHHHHHHHHHHHCCCCHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHC
GIDGVETTRRLKAVSPSSQVIVLTSFADDDKVFPAIKAGAISYLLKDISPEDLAHAIRAA
CCCCHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
CRGEAVLHPDVAAKLMQEFNTPRPNEAPVEQLTPREMDVLRLVAKGMSNKEIAETLIVSE
HCCCCEECHHHHHHHHHHCCCCCCCCCCHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHH
KTVKTHISNILSKLHLADRTQVAIYALRKRLVPIDEEE
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]