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The map label for this gene is truB [H]

Identifier: 222526496

GI number: 222526496

Start: 4069092

End: 4069964

Strand: Direct

Name: truB [H]

Synonym: Chy400_3263

Alternate gene names: 222526496

Gene position: 4069092-4069964 (Clockwise)

Preceding gene: 222526495

Following gene: 222526497

Centisome position: 77.23

GC content: 61.74

Gene sequence:

>873_bases
ATGACAGCATTAACAGGATTTCTGGTTGTCGATAAACCGGCTGGTCTGACATCGCACGATGTGGTGGCCCGCGTGCGCCG
ACTGGCAGGGCGGGCGGTAAAGGTTGGCCACGCCGGTACCCTCGATCCGGCAGCAACGGGTGTATTACCCGTTGCCCTGG
GCAGTGCGACGCGACTGATCGATCAGTTGGTCGATGCCCGGAAGGGCTACCGTGGCGTCGTGCGCCTCGGTGTGCAGACA
ACGACCGATGATGCCGAGGGTGAACCGGTAGCGACCCTGCCGGTACCAGTATTCACGATCACGGAAATCGCCGCTGTCCT
GGCCCAGTTTCGGGGTGACATTCTCCAACAACCTCCCGCCTTTTCGGCCTTGCATGTCGCCGGTCAGCGCGCCTACGCCC
TGGCCCGCGCTGGTGATCAGGTGACGCTAGCGCCGCGCCTCGTGCGGATTGATCGTCTGGAGCTGCTTGATTGGGCCTCA
CCCGATCTCACCATCGAGGTCGAATGCGGCAAAGGGGTCTACATTCGCGCACTGGCCCGCGACATTGGCGCTGCCCTTGG
CTGCGGTGGGCATCTGGTCTCATTACAGCGTACCTTCGTCGGCCCCTTTCGTCTCGCACAGGCGGTACCGCTGGCAAACT
TGACCGATCAGTCGTGCTTACTCAACCATCTCCTGCCGCCGGAAACGGTTCTGCAAGACTGGCCCCTCGTCCAGGTTGAT
GCCGACGATGCCAACCGACTACGTCATGGAATGGCCATTACGGCAACCGAACCCGACGCACCGCATGCACGGGCACACGA
TCCAGCCGGTAAGCTCATCGCACTCCTCAAACGTGATGGTGACAACTGGCGACCGGTAAAGGTGTTGGGGTGA

Upstream 100 bases:

>100_bases
GTGGTCACCGTCAGGCTGCGGGGGCAACGTTGACCATGAGCCTCACTGCGGCAGTGGATGCAGTCCTTCCCGCCCTACGC
GAAGCTGTGCATGGGAAGCA

Downstream 100 bases:

>100_bases
ATATCCGAAACAATCCATAAGCGCGAACGCCTATCCTGCAAGGTACCTGCAACTGATACAAGCCAAAGCCTATGAACGCC
ACTACGCGCCTCTACTACGA

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MTALTGFLVVDKPAGLTSHDVVARVRRLAGRAVKVGHAGTLDPAATGVLPVALGSATRLIDQLVDARKGYRGVVRLGVQT
TTDDAEGEPVATLPVPVFTITEIAAVLAQFRGDILQQPPAFSALHVAGQRAYALARAGDQVTLAPRLVRIDRLELLDWAS
PDLTIEVECGKGVYIRALARDIGAALGCGGHLVSLQRTFVGPFRLAQAVPLANLTDQSCLLNHLLPPETVLQDWPLVQVD
ADDANRLRHGMAITATEPDAPHARAHDPAGKLIALLKRDGDNWRPVKVLG

Sequences:

>Translated_290_residues
MTALTGFLVVDKPAGLTSHDVVARVRRLAGRAVKVGHAGTLDPAATGVLPVALGSATRLIDQLVDARKGYRGVVRLGVQT
TTDDAEGEPVATLPVPVFTITEIAAVLAQFRGDILQQPPAFSALHVAGQRAYALARAGDQVTLAPRLVRIDRLELLDWAS
PDLTIEVECGKGVYIRALARDIGAALGCGGHLVSLQRTFVGPFRLAQAVPLANLTDQSCLLNHLLPPETVLQDWPLVQVD
ADDANRLRHGMAITATEPDAPHARAHDPAGKLIALLKRDGDNWRPVKVLG
>Mature_289_residues
TALTGFLVVDKPAGLTSHDVVARVRRLAGRAVKVGHAGTLDPAATGVLPVALGSATRLIDQLVDARKGYRGVVRLGVQTT
TDDAEGEPVATLPVPVFTITEIAAVLAQFRGDILQQPPAFSALHVAGQRAYALARAGDQVTLAPRLVRIDRLELLDWASP
DLTIEVECGKGVYIRALARDIGAALGCGGHLVSLQRTFVGPFRLAQAVPLANLTDQSCLLNHLLPPETVLQDWPLVQVDA
DDANRLRHGMAITATEPDAPHARAHDPAGKLIALLKRDGDNWRPVKVLG

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=252, Percent_Identity=34.9206349206349, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI4503337, Length=175, Percent_Identity=33.7142857142857, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI215599015, Length=175, Percent_Identity=33.7142857142857, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI2367200, Length=265, Percent_Identity=35.4716981132075, Blast_Score=167, Evalue=7e-43,
Organism=Caenorhabditis elegans, GI17553978, Length=197, Percent_Identity=27.9187817258883, Blast_Score=80, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6323204, Length=260, Percent_Identity=29.6153846153846, Blast_Score=83, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6324037, Length=136, Percent_Identity=33.8235294117647, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281364189, Length=261, Percent_Identity=27.9693486590038, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281364187, Length=261, Percent_Identity=27.9693486590038, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281364185, Length=261, Percent_Identity=27.9693486590038, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281364183, Length=261, Percent_Identity=27.9693486590038, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI62471759, Length=261, Percent_Identity=27.9693486590038, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI17975520, Length=261, Percent_Identity=27.9693486590038, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780 [H]

Pfam domain/function: PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 30815; Mature: 30684

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTALTGFLVVDKPAGLTSHDVVARVRRLAGRAVKVGHAGTLDPAATGVLPVALGSATRLI
CCCEEEEEEEECCCCCCHHHHHHHHHHHCCCEEEECCCCCCCHHHHCCHHHHHCHHHHHH
DQLVDARKGYRGVVRLGVQTTTDDAEGEPVATLPVPVFTITEIAAVLAQFRGDILQQPPA
HHHHHHHCCCCEEEEEEEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCC
FSALHVAGQRAYALARAGDQVTLAPRLVRIDRLELLDWASPDLTIEVECGKGVYIRALAR
CHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHH
DIGAALGCGGHLVSLQRTFVGPFRLAQAVPLANLTDQSCLLNHLLPPETVLQDWPLVQVD
HHHHHHCCCCHHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHCCCCEEEEC
ADDANRLRHGMAITATEPDAPHARAHDPAGKLIALLKRDGDNWRPVKVLG
CCHHHHHHCCEEEEECCCCCCCCCCCCCHHHEEEEEECCCCCCCCEEEEC
>Mature Secondary Structure 
TALTGFLVVDKPAGLTSHDVVARVRRLAGRAVKVGHAGTLDPAATGVLPVALGSATRLI
CCEEEEEEEECCCCCCHHHHHHHHHHHCCCEEEECCCCCCCHHHHCCHHHHHCHHHHHH
DQLVDARKGYRGVVRLGVQTTTDDAEGEPVATLPVPVFTITEIAAVLAQFRGDILQQPPA
HHHHHHHCCCCEEEEEEEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCC
FSALHVAGQRAYALARAGDQVTLAPRLVRIDRLELLDWASPDLTIEVECGKGVYIRALAR
CHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHH
DIGAALGCGGHLVSLQRTFVGPFRLAQAVPLANLTDQSCLLNHLLPPETVLQDWPLVQVD
HHHHHHCCCCHHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHCCCCEEEEC
ADDANRLRHGMAITATEPDAPHARAHDPAGKLIALLKRDGDNWRPVKVLG
CCHHHHHHCCEEEEECCCCCCCCCCCCCHHHEEEEEECCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA