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The map label for this gene is alaXL [H]

Identifier: 222526497

GI number: 222526497

Start: 4070036

End: 4071268

Strand: Direct

Name: alaXL [H]

Synonym: Chy400_3264

Alternate gene names: 222526497

Gene position: 4070036-4071268 (Clockwise)

Preceding gene: 222526496

Following gene: 222526509

Centisome position: 77.25

GC content: 64.4

Gene sequence:

>1233_bases
ATGAACGCCACTACGCGCCTCTACTACGACGACTCCTACCTGACAACGTTCAACGCGCAGGTGATCGCCACGACCGAACG
TCAAGGGCTGCCTGCGGTCGCCCTCGATCAGAGCGCCTTCTACCCAGAGGGTGGTGGTCAACCCGCCGATCAGGGCTGGC
TATCTGCCGCCACCGATCCTGACCGGCGCTGGCCGGTGATTGACGTGCAGAGTGACAACGGCGTTGTCTGGCATATCCTG
GCGACCGGCGTTCAGCTTCCCGAACCGGGAACTATCGTCACCGGTCAGATCAACTGGGAACGCCGCTTCGACCACATGCA
ACAACACTGTGGTCAGCACATCCTCACCGCAGCCTTCATCGCGACGTGCAATGCACCAACCGTCTCCTTCCATCTGAGCG
AGCGCAGCGTCACCATTGATCTCAGCATCGGCGAACTCAGCCTGGATCAGGTCCAGGCTGCCGAAGCCTGGGCCAATCGT
GCCGTGTGGGCAAACCTACCGATCCATGCCCGCTTTGTATCGCCGACCGAACTGGCTCAGATCCCGCTGCGCAAACCACC
GACCGTCAGTGACGCAGTGCGCGTAGTCAGCATTGGTGACATCGATCACTCAGCGTGTGGCGGCACCCATCCGGCCCGCA
GTGGTGAAGTCGGCGCGATTGCCGTTCTGGGGTGGGGGCGACAACGTGGCCTGATCCGGGTCGAGTTTGTGTGTGGGAAC
CGCGTCATTACCGCCCTTCACGAACGGGATACCGCTGTGCGGACGGCTGCCGCAGCGTTGAGCGTGGGCTGGGCAGAACT
TCCGCAGACCGTCAATCGCTTGCAAGAGACCCAGCAATCGCTTCAACGCGAACTGCGCCAGACCCAGCGCGAACTCGATG
CACTGCGGGCAGCGCAGTGGTATGCGCAGACCCCACCCGTCAACGGGCGGCGTATCGTGACCGCCACCCTTCCCGCTGCC
GACGCCGAGCGCCTGCGCACCATTGCCGGCTTTATCGCTGAACTGCCGGGTGGCATTGCCATTGTCGCCGGCGGCAGCGA
ACGACCGCAGATCGTCGTTGCCTGCGCCGAAGACAGTGCCGGCGACGCCCGGCAAATCCTGGCCGCCGGGATGGCCGTCC
TCGGTGGGCGTGGCGGTGGCAATGCCCGCCTTGCTCAGGGCGGTGGCGGCTCACCCGACGCCCTGGCTGCGGCAATGGCA
GCGATGATCGAGCGTGCGGGGGCATCTGAATAG

Upstream 100 bases:

>100_bases
ACTGGCGACCGGTAAAGGTGTTGGGGTGAATATCCGAAACAATCCATAAGCGCGAACGCCTATCCTGCAAGGTACCTGCA
ACTGATACAAGCCAAAGCCT

Downstream 100 bases:

>100_bases
GGAACGCGGATCGGCGCGGATTTGGCGGATAGACGCGGATGATCCGTGAGTATCCGCGTCAACCCGCGTGTATCCGTGTT
CTATAAACAGGACACGCGGA

Product: Threonyl/alanyl tRNA synthetase SAD

Products: AMP; diphosphate; L-alanyl-tRNAAla [C]

Alternate protein names: AlaX-L; Alanyl-tRNA deacylase AlaX-L [H]

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MNATTRLYYDDSYLTTFNAQVIATTERQGLPAVALDQSAFYPEGGGQPADQGWLSAATDPDRRWPVIDVQSDNGVVWHIL
ATGVQLPEPGTIVTGQINWERRFDHMQQHCGQHILTAAFIATCNAPTVSFHLSERSVTIDLSIGELSLDQVQAAEAWANR
AVWANLPIHARFVSPTELAQIPLRKPPTVSDAVRVVSIGDIDHSACGGTHPARSGEVGAIAVLGWGRQRGLIRVEFVCGN
RVITALHERDTAVRTAAAALSVGWAELPQTVNRLQETQQSLQRELRQTQRELDALRAAQWYAQTPPVNGRRIVTATLPAA
DAERLRTIAGFIAELPGGIAIVAGGSERPQIVVACAEDSAGDARQILAAGMAVLGGRGGGNARLAQGGGGSPDALAAAMA
AMIERAGASE

Sequences:

>Translated_410_residues
MNATTRLYYDDSYLTTFNAQVIATTERQGLPAVALDQSAFYPEGGGQPADQGWLSAATDPDRRWPVIDVQSDNGVVWHIL
ATGVQLPEPGTIVTGQINWERRFDHMQQHCGQHILTAAFIATCNAPTVSFHLSERSVTIDLSIGELSLDQVQAAEAWANR
AVWANLPIHARFVSPTELAQIPLRKPPTVSDAVRVVSIGDIDHSACGGTHPARSGEVGAIAVLGWGRQRGLIRVEFVCGN
RVITALHERDTAVRTAAAALSVGWAELPQTVNRLQETQQSLQRELRQTQRELDALRAAQWYAQTPPVNGRRIVTATLPAA
DAERLRTIAGFIAELPGGIAIVAGGSERPQIVVACAEDSAGDARQILAAGMAVLGGRGGGNARLAQGGGGSPDALAAAMA
AMIERAGASE
>Mature_410_residues
MNATTRLYYDDSYLTTFNAQVIATTERQGLPAVALDQSAFYPEGGGQPADQGWLSAATDPDRRWPVIDVQSDNGVVWHIL
ATGVQLPEPGTIVTGQINWERRFDHMQQHCGQHILTAAFIATCNAPTVSFHLSERSVTIDLSIGELSLDQVQAAEAWANR
AVWANLPIHARFVSPTELAQIPLRKPPTVSDAVRVVSIGDIDHSACGGTHPARSGEVGAIAVLGWGRQRGLIRVEFVCGN
RVITALHERDTAVRTAAAALSVGWAELPQTVNRLQETQQSLQRELRQTQRELDALRAAQWYAQTPPVNGRRIVTATLPAA
DAERLRTIAGFIAELPGGIAIVAGGSERPQIVVACAEDSAGDARQILAAGMAVLGGRGGGNARLAQGGGGSPDALAAAMA
AMIERAGASE

Specific function: Functions in trans to edit the amino acid moiety from mischarged charged tRNA(Ala) [H]

COG id: COG2872

COG function: function code R; Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Editing domain AlaX-L subfamily [H]

Homologues:

Organism=Homo sapiens, GI13376886, Length=328, Percent_Identity=28.6585365853659, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI217416402, Length=328, Percent_Identity=28.6585365853659, Blast_Score=122, Evalue=7e-28,
Organism=Escherichia coli, GI1789048, Length=373, Percent_Identity=25.201072386059, Blast_Score=109, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6324288, Length=351, Percent_Identity=25.6410256410256, Blast_Score=94, Evalue=5e-20,
Organism=Drosophila melanogaster, GI18543305, Length=409, Percent_Identity=25.4278728606357, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR003156
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF02272 DHHA1; PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: 6.1.1.7 [C]

Molecular weight: Translated: 43572; Mature: 43572

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNATTRLYYDDSYLTTFNAQVIATTERQGLPAVALDQSAFYPEGGGQPADQGWLSAATDP
CCCCEEEEECCCEEEEECCEEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCCEECCCCC
DRRWPVIDVQSDNGVVWHILATGVQLPEPGTIVTGQINWERRFDHMQQHCGQHILTAAFI
CCCCCEEEEECCCCEEEEEEECCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHH
ATCNAPTVSFHLSERSVTIDLSIGELSLDQVQAAEAWANRAVWANLPIHARFVSPTELAQ
HCCCCCEEEEEECCCEEEEEEEECCCCHHHHHHHHHHHCCCEEECCCEEEEECCHHHHHH
IPLRKPPTVSDAVRVVSIGDIDHSACGGTHPARSGEVGAIAVLGWGRQRGLIRVEFVCGN
CCCCCCCCHHHHEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEEEECC
RVITALHERDTAVRTAAAALSVGWAELPQTVNRLQETQQSLQRELRQTQRELDALRAAQW
EEEEEHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YAQTPPVNGRRIVTATLPAADAERLRTIAGFIAELPGGIAIVAGGSERPQIVVACAEDSA
HCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEECCCCC
GDARQILAAGMAVLGGRGGGNARLAQGGGGSPDALAAAMAAMIERAGASE
CHHHHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNATTRLYYDDSYLTTFNAQVIATTERQGLPAVALDQSAFYPEGGGQPADQGWLSAATDP
CCCCEEEEECCCEEEEECCEEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCCEECCCCC
DRRWPVIDVQSDNGVVWHILATGVQLPEPGTIVTGQINWERRFDHMQQHCGQHILTAAFI
CCCCCEEEEECCCCEEEEEEECCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHH
ATCNAPTVSFHLSERSVTIDLSIGELSLDQVQAAEAWANRAVWANLPIHARFVSPTELAQ
HCCCCCEEEEEECCCEEEEEEEECCCCHHHHHHHHHHHCCCEEECCCEEEEECCHHHHHH
IPLRKPPTVSDAVRVVSIGDIDHSACGGTHPARSGEVGAIAVLGWGRQRGLIRVEFVCGN
CCCCCCCCHHHHEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEEEECC
RVITALHERDTAVRTAAAALSVGWAELPQTVNRLQETQQSLQRELRQTQRELDALRAAQW
EEEEEHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YAQTPPVNGRRIVTATLPAADAERLRTIAGFIAELPGGIAIVAGGSERPQIVVACAEDSA
HCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEECCCCC
GDARQILAAGMAVLGGRGGGNARLAQGGGGSPDALAAAMAAMIERAGASE
CHHHHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; @ALAN01.txt*L-alanine!; tRNAAla [C]

Specific reaction: ATP + @ALAN01.txt*L-alanine! + tRNAAla = AMP + diphosphate + L-alanyl-tRNAAla [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9679194 [H]