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The map label for this gene is infB

Identifier: 222526492

GI number: 222526492

Start: 4064655

End: 4066892

Strand: Direct

Name: infB

Synonym: Chy400_3259

Alternate gene names: 222526492

Gene position: 4064655-4066892 (Clockwise)

Preceding gene: 222526491

Following gene: 222526493

Centisome position: 77.14

GC content: 57.77

Gene sequence:

>2238_bases
ATGAGCGATAAACCACGACGTGATACAGGCAGTACGGGCACAGGCAGTGGACGCAGCACAGGCCAGTCTGGCAGTAATCG
AGCCGTTGGCGCGCCTAACCCTGGTACAGGACGTAGTCCAAATGCTTCGACCGGTGGGAACCGGTCGGCTGGGAATCAAG
CCGGCAATAGTGGTCGTCCGGCTTCCGCTGGACGTAATCAGGCGACTACACCGGCTCCTAATCGAAATACACCTCCTGCT
GGCGCCCGGCAGGGTGGTGCTGCCAATGCGCGTACCGGTACACCACCGGTTGCGCGTGGTGGTGGTGGCGGGGTAACGCC
GCCGACCGGACGCGGTGGGAACAATCCACGCGCAGCACGTAATCAGCCACGCAGCCGCCAGCAACCTGAAGAACGCGAGC
GCGAGCACGTCTTGCGCCGTCCGCCACCTCAACCGGCAGCGCGCCCGGTGGTGCGCCCGCGTGGCCCGGTTGCCTTGCCT
CCGGTAATGACCGTGCGTGAACTTTCGGAAGCGACCGGTATTGGCGCGGCAGATATTCTCAAGGCGATGCTGAAAGCGGG
GATGCTGGCGAATATCAATCAGCAAATTGATTATGAAACCGCTGCACTGATTATGGCCGACTTTGGCATTGAGACGACCG
AAGATGTGCCTGAGCAGATGGCCGGTATCGTTGAAGATGTCAAAGAGGTTTTGCGGGCACAGCCACCCGAAGAGATGCGG
CCACGACCACCGGTGGTGACCATTATGGGTCACGTTGACCACGGTAAGACCAAGCTGCTCGATGCGATCCGCTCAACCCG
TGTCGCCGAGGGTGAGGCCGGTGGCATTACGCAGCATATTGGCGCGTATCAGATTGAGGTTAATCACCGCAAGATCACCT
TCCTGGATACGCCGGGTCACGAAGCATTTACCGCAATGCGTGCCCGTGGTGCTCAGGTGACGGACATTGTGGTGCTGGTG
GTGGCTGCCGATGACGGTGTGAAACCGCAAACCGAAGAGGCGATTGCGCACGTGAAGGCTGCCGGTGTGCCGATGATTGT
GGCGATTAATAAGATTGATCTGCCCACGGCCAACCCTGATCGGATCAAGCAGCAGTTGGCAAACGTTGGAGTGATTGTTG
AAGAGTACGGCGGAAATGTGCCTTGTGTTCACGTTTCGGCACGCCAGAAAATCAATATTGACGGTTTGCTAGAGATGATC
TTGCTGGTTGCCGATCTCGAAGATCTGCGCGCCAATCCAAACGCTCCCGCTGTGGGCACCATCATTGAGGCCAAACTCGA
TAAGAGCCGCGGCCCGGTGGCGACTGTCTTGATCCAGAATGGAACGCTCCATCTGGAGGATAATGTTCTGGTGGGATGCG
TGGCCGGTAAGATCAAGTCGATGTTCAGTGATAGTGGCAAACGTCTGCGCCATGCCGAACCGTCTACACCGGTAGAGATT
GTTGGTCTGGAAGGTGTGCCACAGGCTGGTGATATTTTGCAGGTCATGGATGACCTGGTTGTAGCCCGTGAAATTGCTCT
GCAACGTCAACGCCAGCAACGGGCAGAGGTTATGGCCGCTGCTGCGCGCGGTACCAGCCTCGAAGAGTTGTTCGGCAAGG
TGAAACAGGGCCAGGTGAAAGAGTTGAACCTGATCCTGAAAGCCGATGTCCAGGGTTCGCTCGATGCTATTGCCCATTTG
ATCGAGCAGTTGAACCAGTCGCAGCAGGCCGTACAGACCCGCATCATCCATCGCGGCGTAGGAGCAATTACTGAGGGTGA
TGTCAACCTGGCCCTGGCCAGTCACGCGATCATCATCGGCTTTAATGCGCGCCCCGACCCGGCAGCGCGTCGCCACGCCG
AGCAGCACGGGATCGATATTCGCTTCTACAATATCATCTATCAGTTGCAGGATGATCTGAAGAAGGCGATGGCCGGTATG
CTGGCCCCCACTGTCAAAGAGGTGGTCGAAGGCTATGCCGAGGTACGCAATACCTTCCGCTTGCCAACCCGTGAGGTCGT
CGCCGGTGTCTACGTTTCCGATGGAAAGATCACCCGTACTGGTCAGAATGTGCGTGTGCTACGGCGAGGCGTGGTGATCC
ACGACGGTAAAATCTCGTCACTCAAACGGTTTAAAGATGATGTGCGCGAGGTGACTGCCGGCTACGAGTGCGGTCTGATT
GTCGAGGGCTTCAACGATATCGAAGTCGGTGATGCCCTGGAGTTCTACCGCCAGGAGACGGTTGCCGCCTCGTTGTAG

Upstream 100 bases:

>100_bases
AAGAGACAACTGCTCTAAGCGCCGTTGACGTACACGTGCCTGAACAATGCAGGAGATCGATGGCGGTTTACCATTGCTCT
CCTCAGAAAGGAAGTTGATA

Downstream 100 bases:

>100_bases
GTGCGATAATCGGCAAGATGTTCGCCCGCCTCTGACGCACACCTCACGAGGGGTGTGCGTCAGAGTGACTCGCTGCGTAT
AACGACAGAATCATGACTGG

Product: translation initiation factor IF-2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 745; Mature: 744

Protein sequence:

>745_residues
MSDKPRRDTGSTGTGSGRSTGQSGSNRAVGAPNPGTGRSPNASTGGNRSAGNQAGNSGRPASAGRNQATTPAPNRNTPPA
GARQGGAANARTGTPPVARGGGGGVTPPTGRGGNNPRAARNQPRSRQQPEEREREHVLRRPPPQPAARPVVRPRGPVALP
PVMTVRELSEATGIGAADILKAMLKAGMLANINQQIDYETAALIMADFGIETTEDVPEQMAGIVEDVKEVLRAQPPEEMR
PRPPVVTIMGHVDHGKTKLLDAIRSTRVAEGEAGGITQHIGAYQIEVNHRKITFLDTPGHEAFTAMRARGAQVTDIVVLV
VAADDGVKPQTEEAIAHVKAAGVPMIVAINKIDLPTANPDRIKQQLANVGVIVEEYGGNVPCVHVSARQKINIDGLLEMI
LLVADLEDLRANPNAPAVGTIIEAKLDKSRGPVATVLIQNGTLHLEDNVLVGCVAGKIKSMFSDSGKRLRHAEPSTPVEI
VGLEGVPQAGDILQVMDDLVVAREIALQRQRQQRAEVMAAAARGTSLEELFGKVKQGQVKELNLILKADVQGSLDAIAHL
IEQLNQSQQAVQTRIIHRGVGAITEGDVNLALASHAIIIGFNARPDPAARRHAEQHGIDIRFYNIIYQLQDDLKKAMAGM
LAPTVKEVVEGYAEVRNTFRLPTREVVAGVYVSDGKITRTGQNVRVLRRGVVIHDGKISSLKRFKDDVREVTAGYECGLI
VEGFNDIEVGDALEFYRQETVAASL

Sequences:

>Translated_745_residues
MSDKPRRDTGSTGTGSGRSTGQSGSNRAVGAPNPGTGRSPNASTGGNRSAGNQAGNSGRPASAGRNQATTPAPNRNTPPA
GARQGGAANARTGTPPVARGGGGGVTPPTGRGGNNPRAARNQPRSRQQPEEREREHVLRRPPPQPAARPVVRPRGPVALP
PVMTVRELSEATGIGAADILKAMLKAGMLANINQQIDYETAALIMADFGIETTEDVPEQMAGIVEDVKEVLRAQPPEEMR
PRPPVVTIMGHVDHGKTKLLDAIRSTRVAEGEAGGITQHIGAYQIEVNHRKITFLDTPGHEAFTAMRARGAQVTDIVVLV
VAADDGVKPQTEEAIAHVKAAGVPMIVAINKIDLPTANPDRIKQQLANVGVIVEEYGGNVPCVHVSARQKINIDGLLEMI
LLVADLEDLRANPNAPAVGTIIEAKLDKSRGPVATVLIQNGTLHLEDNVLVGCVAGKIKSMFSDSGKRLRHAEPSTPVEI
VGLEGVPQAGDILQVMDDLVVAREIALQRQRQQRAEVMAAAARGTSLEELFGKVKQGQVKELNLILKADVQGSLDAIAHL
IEQLNQSQQAVQTRIIHRGVGAITEGDVNLALASHAIIIGFNARPDPAARRHAEQHGIDIRFYNIIYQLQDDLKKAMAGM
LAPTVKEVVEGYAEVRNTFRLPTREVVAGVYVSDGKITRTGQNVRVLRRGVVIHDGKISSLKRFKDDVREVTAGYECGLI
VEGFNDIEVGDALEFYRQETVAASL
>Mature_744_residues
SDKPRRDTGSTGTGSGRSTGQSGSNRAVGAPNPGTGRSPNASTGGNRSAGNQAGNSGRPASAGRNQATTPAPNRNTPPAG
ARQGGAANARTGTPPVARGGGGGVTPPTGRGGNNPRAARNQPRSRQQPEEREREHVLRRPPPQPAARPVVRPRGPVALPP
VMTVRELSEATGIGAADILKAMLKAGMLANINQQIDYETAALIMADFGIETTEDVPEQMAGIVEDVKEVLRAQPPEEMRP
RPPVVTIMGHVDHGKTKLLDAIRSTRVAEGEAGGITQHIGAYQIEVNHRKITFLDTPGHEAFTAMRARGAQVTDIVVLVV
AADDGVKPQTEEAIAHVKAAGVPMIVAINKIDLPTANPDRIKQQLANVGVIVEEYGGNVPCVHVSARQKINIDGLLEMIL
LVADLEDLRANPNAPAVGTIIEAKLDKSRGPVATVLIQNGTLHLEDNVLVGCVAGKIKSMFSDSGKRLRHAEPSTPVEIV
GLEGVPQAGDILQVMDDLVVAREIALQRQRQQRAEVMAAAARGTSLEELFGKVKQGQVKELNLILKADVQGSLDAIAHLI
EQLNQSQQAVQTRIIHRGVGAITEGDVNLALASHAIIIGFNARPDPAARRHAEQHGIDIRFYNIIYQLQDDLKKAMAGML
APTVKEVVEGYAEVRNTFRLPTREVVAGVYVSDGKITRTGQNVRVLRRGVVIHDGKISSLKRFKDDVREVTAGYECGLIV
EGFNDIEVGDALEFYRQETVAASL

Specific function: One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 7

COG id: COG0532

COG function: function code J; Translation initiation factor 2 (IF-2; GTPase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IF-2 family

Homologues:

Organism=Homo sapiens, GI53729339, Length=552, Percent_Identity=39.4927536231884, Blast_Score=355, Evalue=1e-97,
Organism=Homo sapiens, GI53729337, Length=552, Percent_Identity=39.4927536231884, Blast_Score=355, Evalue=1e-97,
Organism=Homo sapiens, GI84043963, Length=485, Percent_Identity=27.2164948453608, Blast_Score=118, Evalue=2e-26,
Organism=Homo sapiens, GI157426893, Length=224, Percent_Identity=26.7857142857143, Blast_Score=76, Evalue=1e-13,
Organism=Escherichia coli, GI1789559, Length=607, Percent_Identity=49.2586490939045, Blast_Score=533, Evalue=1e-152,
Organism=Escherichia coli, GI2367247, Length=164, Percent_Identity=36.5853658536585, Blast_Score=80, Evalue=7e-16,
Organism=Escherichia coli, GI1788922, Length=228, Percent_Identity=28.9473684210526, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI48994988, Length=164, Percent_Identity=33.5365853658537, Blast_Score=74, Evalue=5e-14,
Organism=Escherichia coli, GI1790412, Length=133, Percent_Identity=36.0902255639098, Blast_Score=62, Evalue=1e-10,
Organism=Escherichia coli, GI1789737, Length=133, Percent_Identity=36.0902255639098, Blast_Score=62, Evalue=1e-10,
Organism=Caenorhabditis elegans, GI71994658, Length=548, Percent_Identity=34.6715328467153, Blast_Score=288, Evalue=9e-78,
Organism=Caenorhabditis elegans, GI212656558, Length=161, Percent_Identity=38.5093167701863, Blast_Score=109, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17557151, Length=217, Percent_Identity=30.4147465437788, Blast_Score=93, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6324550, Length=537, Percent_Identity=38.1750465549348, Blast_Score=311, Evalue=2e-85,
Organism=Saccharomyces cerevisiae, GI6319282, Length=130, Percent_Identity=43.8461538461538, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6324761, Length=169, Percent_Identity=31.9526627218935, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI28572034, Length=615, Percent_Identity=38.5365853658537, Blast_Score=358, Evalue=8e-99,
Organism=Drosophila melanogaster, GI160714833, Length=76, Percent_Identity=69.7368421052632, Blast_Score=115, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24656849, Length=470, Percent_Identity=25.3191489361702, Blast_Score=111, Evalue=2e-24,
Organism=Drosophila melanogaster, GI78706572, Length=252, Percent_Identity=28.5714285714286, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI19922690, Length=174, Percent_Identity=32.183908045977, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: 1150 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): IF2_CHLAA (A9WGP6)

Other databases:

- EMBL:   CP000909
- RefSeq:   YP_001636601.1
- ProteinModelPortal:   A9WGP6
- SMR:   A9WGP6
- GeneID:   5827487
- GenomeReviews:   CP000909_GR
- KEGG:   cau:Caur_3013
- HOGENOM:   HBG635083
- OMA:   NIFENMG
- ProtClustDB:   PRK05306
- GO:   GO:0005737
- HAMAP:   MF_00100_B
- InterPro:   IPR006847
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000178
- InterPro:   IPR015760
- InterPro:   IPR023115
- InterPro:   IPR004161
- InterPro:   IPR009000
- Gene3D:   G3DSA:3.40.50.10050
- PANTHER:   PTHR23115:SF41
- TIGRFAMs:   TIGR00487
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF11987 IF-2; PF04760 IF2_N; SSF52156 TIF_IF2_dom3; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 79372; Mature: 79241

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: PS01176 IF2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDKPRRDTGSTGTGSGRSTGQSGSNRAVGAPNPGTGRSPNASTGGNRSAGNQAGNSGRP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ASAGRNQATTPAPNRNTPPAGARQGGAANARTGTPPVARGGGGGVTPPTGRGGNNPRAAR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHC
NQPRSRQQPEEREREHVLRRPPPQPAARPVVRPRGPVALPPVMTVRELSEATGIGAADIL
CCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHH
KAMLKAGMLANINQQIDYETAALIMADFGIETTEDVPEQMAGIVEDVKEVLRAQPPEEMR
HHHHHCCHHHCCCHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHC
PRPPVVTIMGHVDHGKTKLLDAIRSTRVAEGEAGGITQHIGAYQIEVNHRKITFLDTPGH
CCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHCCEEEEEECCEEEEEEECCCH
EAFTAMRARGAQVTDIVVLVVAADDGVKPQTEEAIAHVKAAGVPMIVAINKIDLPTANPD
HHHHHHHHCCCEEEEEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEEEEEECCCCCCHH
RIKQQLANVGVIVEEYGGNVPCVHVSARQKINIDGLLEMILLVADLEDLRANPNAPAVGT
HHHHHHHHCCEEEECCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHH
IIEAKLDKSRGPVATVLIQNGTLHLEDNVLVGCVAGKIKSMFSDSGKRLRHAEPSTPVEI
HHHHHHCCCCCCEEEEEEECCEEEEECCEEEEHHHHHHHHHHHCCCCHHHCCCCCCCEEE
VGLEGVPQAGDILQVMDDLVVAREIALQRQRQQRAEVMAAAARGTSLEELFGKVKQGQVK
EECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCE
ELNLILKADVQGSLDAIAHLIEQLNQSQQAVQTRIIHRGVGAITEGDVNLALASHAIIIG
EEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCEEEEE
FNARPDPAARRHAEQHGIDIRFYNIIYQLQDDLKKAMAGMLAPTVKEVVEGYAEVRNTFR
ECCCCCHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LPTREVVAGVYVSDGKITRTGQNVRVLRRGVVIHDGKISSLKRFKDDVREVTAGYECGLI
CCHHHHHEEEEEECCEEEECCCHHHHHHCCEEEECCCHHHHHHHHHHHHHHHCCCCCCEE
VEGFNDIEVGDALEFYRQETVAASL
EECCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDKPRRDTGSTGTGSGRSTGQSGSNRAVGAPNPGTGRSPNASTGGNRSAGNQAGNSGRP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ASAGRNQATTPAPNRNTPPAGARQGGAANARTGTPPVARGGGGGVTPPTGRGGNNPRAAR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHC
NQPRSRQQPEEREREHVLRRPPPQPAARPVVRPRGPVALPPVMTVRELSEATGIGAADIL
CCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHH
KAMLKAGMLANINQQIDYETAALIMADFGIETTEDVPEQMAGIVEDVKEVLRAQPPEEMR
HHHHHCCHHHCCCHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHC
PRPPVVTIMGHVDHGKTKLLDAIRSTRVAEGEAGGITQHIGAYQIEVNHRKITFLDTPGH
CCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHCCEEEEEECCEEEEEEECCCH
EAFTAMRARGAQVTDIVVLVVAADDGVKPQTEEAIAHVKAAGVPMIVAINKIDLPTANPD
HHHHHHHHCCCEEEEEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEEEEEECCCCCCHH
RIKQQLANVGVIVEEYGGNVPCVHVSARQKINIDGLLEMILLVADLEDLRANPNAPAVGT
HHHHHHHHCCEEEECCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHH
IIEAKLDKSRGPVATVLIQNGTLHLEDNVLVGCVAGKIKSMFSDSGKRLRHAEPSTPVEI
HHHHHHCCCCCCEEEEEEECCEEEEECCEEEEHHHHHHHHHHHCCCCHHHCCCCCCCEEE
VGLEGVPQAGDILQVMDDLVVAREIALQRQRQQRAEVMAAAARGTSLEELFGKVKQGQVK
EECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCE
ELNLILKADVQGSLDAIAHLIEQLNQSQQAVQTRIIHRGVGAITEGDVNLALASHAIIIG
EEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCEEEEE
FNARPDPAARRHAEQHGIDIRFYNIIYQLQDDLKKAMAGMLAPTVKEVVEGYAEVRNTFR
ECCCCCHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LPTREVVAGVYVSDGKITRTGQNVRVLRRGVVIHDGKISSLKRFKDDVREVTAGYECGLI
CCHHHHHEEEEEECCEEEECCCHHHHHHCCEEEECCCHHHHHHHHHHHHHHHCCCCCCEE
VEGFNDIEVGDALEFYRQETVAASL
EECCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; In phosphorus-containing anhydrides [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA