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The map label for this gene is rlmN

Identifier: 222526414

GI number: 222526414

Start: 3940753

End: 3941883

Strand: Direct

Name: rlmN

Synonym: Chy400_3180

Alternate gene names: 222526414

Gene position: 3940753-3941883 (Clockwise)

Preceding gene: 222526412

Following gene: 222526415

Centisome position: 74.79

GC content: 57.21

Gene sequence:

>1131_bases
ATGCACTATGCTATGGAACAACGCTGTTTGTATGATTACAACCTGTCTGAACTGACCGAACTCCTGCAAAGCTGGGGAGA
ACCGGCCTTTCGTGCCCGTCAACTGTACCGGCATCTGTATGTCAACCTTGCCCGCCAGGTTGATCAGATGACCGATCTGC
CGCTCGCTCTGCGTTCCCGGCTCGCCGAGATACCGTTCTCGACTCTCCGCTGCGAACAGGTGCAGATCGGTGACAATGGG
ATGACCCGTAAGGCCCTCTTCCGCTTGCCCGACGGTGCTGTTGTTGAAACCGTATTGATGGTCTATCCCGACCGCTCAAC
GGTATGTGTGTCAACGCAGGCCGGTTGCGGTATGGGCTGCGTCTTCTGTGCCACCGGTCAGCTCGGTCTTCTGCGCAACC
TGAGCAGTGGCGAAATCGTTGCTCAGGCAATTTGGGCTTCACAGGAGCTACGGGCGATGGGTATGGCCGGTCCGACCGGT
CGCGTCAGTAACCTTGTTTTTATGGGGATGGGTGAACCATTTGCCAACTACGACCGCTGGTGGCAGGCGGTAGAGCGATT
GCACGATCCGCAGGGGTTTAACCTCGGTGCCCGCAGTATGACCGTCTCCACTGTAGGGCTGGTCAAGGGGATCGAGCGAT
TGGCCAATGAACGCTTACCGATCAATCTGGCAATTTCGCTGCATGCGCCCGATGACGCTCTGCGCAGCGAATTGATGCCG
GTCAATCGGCGTTATCCAATCGCCGATCTGATGGCTGCCACCCGCAACTATATTGCCAAAACGCGCCGGCGAGTCTCTTT
CGAGTATGTGCTGTTGCAAGGCAAAAACGACCATCCGCATCAGGCAATCGCACTAGCCCGCCTGCTCCGACATTCGGCAC
CGCGTGGTCCGCTGCTCTTTCACGTCAATCTGATACCGTGGAACCCGGTTCCTGGCACGCCGCTTGGCCGTTCAGAGTGG
GAGCGTGTGACGACGTTTCAGCAAATTTTGACCGATTACGGCATTCCCTGTACCGTGCGGGTTGAGCGAGGGGTAGAAAT
TGCTGCGGCGTGTGGTCAGTTGGCCGGTCGGCATAGTGTGCCACTGAACACGGTAGAACAAGAAATACCAGCACGCAACG
TTACCGGATGA

Upstream 100 bases:

>100_bases
CTCTTTCACTAAACAACTACCGACTCTGAAACGCCGGCACGTGCGATTTGGTTCCCATTGGTAACAAACTGCTGCGCTCG
CCTTACGGCAACGTGGTACA

Downstream 100 bases:

>100_bases
CACCTGCGAGTTCGAGATTCCTCTTTGTACACAGACGAGAGTTGTATGGCGCCGATTCAAAATGTTAAAGGAATGCGCGA
TCATCTGCCGGCAGATATGA

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MHYAMEQRCLYDYNLSELTELLQSWGEPAFRARQLYRHLYVNLARQVDQMTDLPLALRSRLAEIPFSTLRCEQVQIGDNG
MTRKALFRLPDGAVVETVLMVYPDRSTVCVSTQAGCGMGCVFCATGQLGLLRNLSSGEIVAQAIWASQELRAMGMAGPTG
RVSNLVFMGMGEPFANYDRWWQAVERLHDPQGFNLGARSMTVSTVGLVKGIERLANERLPINLAISLHAPDDALRSELMP
VNRRYPIADLMAATRNYIAKTRRRVSFEYVLLQGKNDHPHQAIALARLLRHSAPRGPLLFHVNLIPWNPVPGTPLGRSEW
ERVTTFQQILTDYGIPCTVRVERGVEIAAACGQLAGRHSVPLNTVEQEIPARNVTG

Sequences:

>Translated_376_residues
MHYAMEQRCLYDYNLSELTELLQSWGEPAFRARQLYRHLYVNLARQVDQMTDLPLALRSRLAEIPFSTLRCEQVQIGDNG
MTRKALFRLPDGAVVETVLMVYPDRSTVCVSTQAGCGMGCVFCATGQLGLLRNLSSGEIVAQAIWASQELRAMGMAGPTG
RVSNLVFMGMGEPFANYDRWWQAVERLHDPQGFNLGARSMTVSTVGLVKGIERLANERLPINLAISLHAPDDALRSELMP
VNRRYPIADLMAATRNYIAKTRRRVSFEYVLLQGKNDHPHQAIALARLLRHSAPRGPLLFHVNLIPWNPVPGTPLGRSEW
ERVTTFQQILTDYGIPCTVRVERGVEIAAACGQLAGRHSVPLNTVEQEIPARNVTG
>Mature_376_residues
MHYAMEQRCLYDYNLSELTELLQSWGEPAFRARQLYRHLYVNLARQVDQMTDLPLALRSRLAEIPFSTLRCEQVQIGDNG
MTRKALFRLPDGAVVETVLMVYPDRSTVCVSTQAGCGMGCVFCATGQLGLLRNLSSGEIVAQAIWASQELRAMGMAGPTG
RVSNLVFMGMGEPFANYDRWWQAVERLHDPQGFNLGARSMTVSTVGLVKGIERLANERLPINLAISLHAPDDALRSELMP
VNRRYPIADLMAATRNYIAKTRRRVSFEYVLLQGKNDHPHQAIALARLLRHSAPRGPLLFHVNLIPWNPVPGTPLGRSEW
ERVTTFQQILTDYGIPCTVRVERGVEIAAACGQLAGRHSVPLNTVEQEIPARNVTG

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=355, Percent_Identity=39.4366197183099, Blast_Score=245, Evalue=3e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_CHLAA (A9WFY6)

Other databases:

- EMBL:   CP000909
- RefSeq:   YP_001636529.1
- ProteinModelPortal:   A9WFY6
- SMR:   A9WFY6
- GeneID:   5827413
- GenomeReviews:   CP000909_GR
- KEGG:   cau:Caur_2941
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- ProtClustDB:   PRK14461
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 41977; Mature: 41977

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHYAMEQRCLYDYNLSELTELLQSWGEPAFRARQLYRHLYVNLARQVDQMTDLPLALRSR
CCCCHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LAEIPFSTLRCEQVQIGDNGMTRKALFRLPDGAVVETVLMVYPDRSTVCVSTQAGCGMGC
HHHCCHHHHEEEEEEECCCCCCHHHHHCCCCCHHHHHHHHEECCCCEEEEECCCCCCCCE
VFCATGQLGLLRNLSSGEIVAQAIWASQELRAMGMAGPTGRVSNLVFMGMGEPFANYDRW
EEECCCCHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHH
WQAVERLHDPQGFNLGARSMTVSTVGLVKGIERLANERLPINLAISLHAPDDALRSELMP
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCHHHHHHHCCC
VNRRYPIADLMAATRNYIAKTRRRVSFEYVLLQGKNDHPHQAIALARLLRHSAPRGPLLF
CCCCCCHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEE
HVNLIPWNPVPGTPLGRSEWERVTTFQQILTDYGIPCTVRVERGVEIAAACGQLAGRHSV
EEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCC
PLNTVEQEIPARNVTG
CCHHHHHHCCCCCCCC
>Mature Secondary Structure
MHYAMEQRCLYDYNLSELTELLQSWGEPAFRARQLYRHLYVNLARQVDQMTDLPLALRSR
CCCCHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LAEIPFSTLRCEQVQIGDNGMTRKALFRLPDGAVVETVLMVYPDRSTVCVSTQAGCGMGC
HHHCCHHHHEEEEEEECCCCCCHHHHHCCCCCHHHHHHHHEECCCCEEEEECCCCCCCCE
VFCATGQLGLLRNLSSGEIVAQAIWASQELRAMGMAGPTGRVSNLVFMGMGEPFANYDRW
EEECCCCHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHH
WQAVERLHDPQGFNLGARSMTVSTVGLVKGIERLANERLPINLAISLHAPDDALRSELMP
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCHHHHHHHCCC
VNRRYPIADLMAATRNYIAKTRRRVSFEYVLLQGKNDHPHQAIALARLLRHSAPRGPLLF
CCCCCCHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEE
HVNLIPWNPVPGTPLGRSEWERVTTFQQILTDYGIPCTVRVERGVEIAAACGQLAGRHSV
EEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCC
PLNTVEQEIPARNVTG
CCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA