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The map label for this gene is hisS [H]

Identifier: 222526415

GI number: 222526415

Start: 3941929

End: 3943245

Strand: Direct

Name: hisS [H]

Synonym: Chy400_3181

Alternate gene names: 222526415

Gene position: 3941929-3943245 (Clockwise)

Preceding gene: 222526414

Following gene: 222526417

Centisome position: 74.81

GC content: 57.25

Gene sequence:

>1317_bases
ATGGCGCCGATTCAAAATGTTAAAGGAATGCGCGATCATCTGCCGGCAGATATGATGCTACGGCAATACATCGTCAATAC
GCTGACGCGCATTAGTGAAAGTCACGGCTTCGAGCCTTTGCAGACCCCGATCATCGAGTATGCCGAGACGCTGGAAGGCA
AGCTGGGTGATGAAGAAAAGCTGATTTACCGGTTTGAAGACCATGGTGGCCGCCGTCTGGCTCTGCGCTACGATCAGACG
GTGCCGTTGGCGCGTGTGACTGCTCAATACGCCGGGCAATTAACATTACCCTGGCGCCGATATGCGTATGGGCCGAGCTA
TCGCGGTGAACGCCCCGCGCGTGGTCGGTATCGAGAGTTTTACCAGTTTGATCTCGATATTGTCGGGAGTGCCTCGCCAT
TGGCCGATGCCGAGATTGTGGCGATGTTGTGTGAAGCCCTGACTGCGCTTGGTTTTCCCGGTTTTGTCACGCTGTTAAAT
CACCGTCAGATCATCGGTGGTATTGCCCGTGTGAGCGGTTTGTCCGAAGAGGCTGCCGGCGGTGTCTATCGTGCCATCGA
TAAGTTCGACAAGATTGGCGCTGATGGTGTGCGGGCTGAGTTGATCAAGTCTGGTGTGACTCCTGCTGCGGCTGACCAGA
TTCTGGAGCTGGTACAAATTGATGGTACGGCAGATGAAGTTCTGGATGCACTTGCCCAAAAACTGGCCGCCGATGAGCGA
GCAGTAGCAGCGATTGCCAATCTGCGTGCCGTCCTGGACGGTCTGATGGCGATGGGGGTTCCGGCTGAGCAGTATCGGGT
GGCTCCTCGCCTGGCGCGCGGTCTTTCGTACTACACAGGAGTCGTCTTTGAGGCGATTACCCCGCACTGGCCGGAGGGTT
CACTGCTTGGAGGTGGTCGCTACGATCACCTGATCGGGCTGTTTGCCGGTCGCAACATTCCTACGGTTGGGCTGGCCTTC
GGTATTGACCGCCTGCACGATGTGATGATCGAGCTAAACCTCGGCCCGCGTCCGCAGACAACGGCTCTCGCATTTGTGAC
GTTGTTCGGCGCAGAACAGGTTGCCGAGAGTCTGAGGCTGGCGGCTGAGCTGCGGGCAGCAGGTATCAATACCCTTATCG
CACTTGAACCAGGGAACATCGGTAAACAGTTCAAGGAGGCTGACCGGCGTGGGGTGCGGTTTGCTCTGGTCCTCGGCCCC
GACGAACTGGCCCGTGGTGAGGTTGTGATCAAAGATTTACAACGCGGTGAACAACGCTCGCTTCCCCGTGCAGCGGCTAT
CAGTCTGCTGGCTGAGGCGTTGAGCGAGGGTAAGTAA

Upstream 100 bases:

>100_bases
TGTGCCACTGAACACGGTAGAACAAGAAATACCAGCACGCAACGTTACCGGATGACACCTGCGAGTTCGAGATTCCTCTT
TGTACACAGACGAGAGTTGT

Downstream 100 bases:

>100_bases
GTGTCACGTCTCGGCAGGTTAAAACCCTGCCTCAATGCGTGGAAGGCCGGCGGGGCTGGCGGCGCAGAAAGGTGCCGCCA
GCCCCGTCTATGCATTACCC

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MAPIQNVKGMRDHLPADMMLRQYIVNTLTRISESHGFEPLQTPIIEYAETLEGKLGDEEKLIYRFEDHGGRRLALRYDQT
VPLARVTAQYAGQLTLPWRRYAYGPSYRGERPARGRYREFYQFDLDIVGSASPLADAEIVAMLCEALTALGFPGFVTLLN
HRQIIGGIARVSGLSEEAAGGVYRAIDKFDKIGADGVRAELIKSGVTPAAADQILELVQIDGTADEVLDALAQKLAADER
AVAAIANLRAVLDGLMAMGVPAEQYRVAPRLARGLSYYTGVVFEAITPHWPEGSLLGGGRYDHLIGLFAGRNIPTVGLAF
GIDRLHDVMIELNLGPRPQTTALAFVTLFGAEQVAESLRLAAELRAAGINTLIALEPGNIGKQFKEADRRGVRFALVLGP
DELARGEVVIKDLQRGEQRSLPRAAAISLLAEALSEGK

Sequences:

>Translated_438_residues
MAPIQNVKGMRDHLPADMMLRQYIVNTLTRISESHGFEPLQTPIIEYAETLEGKLGDEEKLIYRFEDHGGRRLALRYDQT
VPLARVTAQYAGQLTLPWRRYAYGPSYRGERPARGRYREFYQFDLDIVGSASPLADAEIVAMLCEALTALGFPGFVTLLN
HRQIIGGIARVSGLSEEAAGGVYRAIDKFDKIGADGVRAELIKSGVTPAAADQILELVQIDGTADEVLDALAQKLAADER
AVAAIANLRAVLDGLMAMGVPAEQYRVAPRLARGLSYYTGVVFEAITPHWPEGSLLGGGRYDHLIGLFAGRNIPTVGLAF
GIDRLHDVMIELNLGPRPQTTALAFVTLFGAEQVAESLRLAAELRAAGINTLIALEPGNIGKQFKEADRRGVRFALVLGP
DELARGEVVIKDLQRGEQRSLPRAAAISLLAEALSEGK
>Mature_437_residues
APIQNVKGMRDHLPADMMLRQYIVNTLTRISESHGFEPLQTPIIEYAETLEGKLGDEEKLIYRFEDHGGRRLALRYDQTV
PLARVTAQYAGQLTLPWRRYAYGPSYRGERPARGRYREFYQFDLDIVGSASPLADAEIVAMLCEALTALGFPGFVTLLNH
RQIIGGIARVSGLSEEAAGGVYRAIDKFDKIGADGVRAELIKSGVTPAAADQILELVQIDGTADEVLDALAQKLAADERA
VAAIANLRAVLDGLMAMGVPAEQYRVAPRLARGLSYYTGVVFEAITPHWPEGSLLGGGRYDHLIGLFAGRNIPTVGLAFG
IDRLHDVMIELNLGPRPQTTALAFVTLFGAEQVAESLRLAAELRAAGINTLIALEPGNIGKQFKEADRRGVRFALVLGPD
ELARGEVVIKDLQRGEQRSLPRAAAISLLAEALSEGK

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI6996014, Length=440, Percent_Identity=33.4090909090909, Blast_Score=234, Evalue=9e-62,
Organism=Homo sapiens, GI15029520, Length=455, Percent_Identity=32.5274725274725, Blast_Score=226, Evalue=4e-59,
Organism=Escherichia coli, GI1788861, Length=435, Percent_Identity=30.1149425287356, Blast_Score=172, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI71993686, Length=459, Percent_Identity=32.8976034858388, Blast_Score=211, Evalue=7e-55,
Organism=Caenorhabditis elegans, GI71993693, Length=459, Percent_Identity=32.8976034858388, Blast_Score=210, Evalue=9e-55,
Organism=Saccharomyces cerevisiae, GI6325290, Length=450, Percent_Identity=32.4444444444444, Blast_Score=226, Evalue=6e-60,
Organism=Drosophila melanogaster, GI24643061, Length=457, Percent_Identity=33.6980306345733, Blast_Score=215, Evalue=4e-56,
Organism=Drosophila melanogaster, GI24643059, Length=476, Percent_Identity=32.3529411764706, Blast_Score=204, Evalue=1e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 47624; Mature: 47493

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPIQNVKGMRDHLPADMMLRQYIVNTLTRISESHGFEPLQTPIIEYAETLEGKLGDEEK
CCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCC
LIYRFEDHGGRRLALRYDQTVPLARVTAQYAGQLTLPWRRYAYGPSYRGERPARGRYREF
EEEEEECCCCCEEEEEECCCCCHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCHHHHH
YQFDLDIVGSASPLADAEIVAMLCEALTALGFPGFVTLLNHRQIIGGIARVSGLSEEAAG
HHHCHHEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCC
GVYRAIDKFDKIGADGVRAELIKSGVTPAAADQILELVQIDGTADEVLDALAQKLAADER
HHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHH
AVAAIANLRAVLDGLMAMGVPAEQYRVAPRLARGLSYYTGVVFEAITPHWPEGSLLGGGR
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCC
YDHLIGLFAGRNIPTVGLAFGIDRLHDVMIELNLGPRPQTTALAFVTLFGAEQVAESLRL
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
AAELRAAGINTLIALEPGNIGKQFKEADRRGVRFALVLGPDELARGEVVIKDLQRGEQRS
HHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEECCHHHHCCHHHHHHHHCCHHHC
LPRAAAISLLAEALSEGK
CCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
APIQNVKGMRDHLPADMMLRQYIVNTLTRISESHGFEPLQTPIIEYAETLEGKLGDEEK
CCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCC
LIYRFEDHGGRRLALRYDQTVPLARVTAQYAGQLTLPWRRYAYGPSYRGERPARGRYREF
EEEEEECCCCCEEEEEECCCCCHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCHHHHH
YQFDLDIVGSASPLADAEIVAMLCEALTALGFPGFVTLLNHRQIIGGIARVSGLSEEAAG
HHHCHHEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCC
GVYRAIDKFDKIGADGVRAELIKSGVTPAAADQILELVQIDGTADEVLDALAQKLAADER
HHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHH
AVAAIANLRAVLDGLMAMGVPAEQYRVAPRLARGLSYYTGVVFEAITPHWPEGSLLGGGR
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCC
YDHLIGLFAGRNIPTVGLAFGIDRLHDVMIELNLGPRPQTTALAFVTLFGAEQVAESLRL
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
AAELRAAGINTLIALEPGNIGKQFKEADRRGVRFALVLGPDELARGEVVIKDLQRGEQRS
HHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEECCHHHHCCHHHHHHHHCCHHHC
LPRAAAISLLAEALSEGK
CCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12835416 [H]