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The map label for this gene is nqo13 [H]

Identifier: 222526380

GI number: 222526380

Start: 3890881

End: 3892458

Strand: Direct

Name: nqo13 [H]

Synonym: Chy400_3146

Alternate gene names: 222526380

Gene position: 3890881-3892458 (Clockwise)

Preceding gene: 222526379

Following gene: 222526381

Centisome position: 73.85

GC content: 55.58

Gene sequence:

>1578_bases
ATGAATCAGCTAGGCTTTCCACTCCTCTCGCTCATTTTGTGGCTACCGACCGTCGGCGCGTTGGTGCTCTTGTTTGTACC
ACGCACGAATGACGAGCTGGCCCGCCGGGTATCACTGGTAACAATGATCATCGGTTTCTTGCTCTCGTTGCTGCTGCCGT
TGCGCTTCGAGACAAATCCCATGCAGATTGCGGCCGTTGGTGCGCCACCGGTGATGCAATTTGTCGAAGAACTTCCCTGG
CTCCCGATTGTTGGCGCGACGTACAGCCTGGGGATTGATGGCATTAGCCTCTGGCTGGTGATGCTGACCACCTTCCTCGG
CCCGATTGTAGTGCTCTCGACCTGGGACTCGGTGCATAAAGATGTGCGTAACTTCCAGATTCTGCTGCTCGTTCTCCAGA
CGGCCATGATCGGCGTCTTTATTGCTCAGGATCTGTTGTTGTTCTACCTCTTCTGGGAGTTTACGCTCATTCCGATGACG
TTCCTCATCGGTATCTGGGGAAGCCAGAATCGCATTTATGCCGCACGCAAATTCTTTCTCTACACGTTTGCCGGCTCGGT
GCTCATGCTGCTGGCTTTAATCGCATTGCACATTCTCCATCGTGATGCGATTGCGGCAAGCGATCCCAATTTCCGCGGTA
CATTCAGTTTCAGTCAGTTTGTGAGCGACATGCGGGCCGGTCGGTTTACCCTCGATACCGTTACAGAGCGACTGTTATTT
GGTGCTTTCTTCCTGGCCTTTGCGGTAAAAGTGCCGCTCTGGCCATTCCATACCTGGCTACCTGATGCACACGTCGAGGC
GCCAACGGCCGGTTCGGTGGTGCTGGCCGGTGTTCTGCTCAAGCTGGGTGGCTACGGTATAATTCGTTACAACCTGACCC
TCTTCCCGGCAGCGGCGCAGTGGGCCGCGCCGGCACTGGCGATCCTGGCCGTTATTGGCATTATTTACGGGGCCGCGGTC
GCCTTTGCGCAATCGGACATGAAAAGGCTGGTCGCTTACTCTTCGGTCAGTCATATGGGTTTTGTGGTGCTGGGCATCTT
TGCACTGAACGCAGAAGGCATAAGCGGCGCAGTATTGCAGATGGTTAACCATGGCCTCAGCACCAGTGCCCTCTTCTTGA
TGGTTGGTGTGCTCTACGAGCGCCGACACACCCGTGACCTGGCCGAATTTGGTGGATTGTGGAAGGTGATGCCGGTCTAT
GCAGCGTTTAGCTTGCTGGTTGCGCTTTCATCGGCCGGCTTGCCGGGATTAAACGGTTTTGTTGGCGAATTTACGATTAT
TACCGGTGCCTTCCGGTCGCCACTGCTGGGATGGGTCTACGTTGCCTTCGCGGTAGGCGGTGTGGTGCTGGCCGCAGCCT
ATCTGCTCAAACTCTTTCGGTCTGTCTTTATGGGTGAAGTCTCGCAGCCGGCTAATGCCAGCCTGCCTGACCTGAATCGC
CGCGAGTTAACGGCCTTTGCTCTGTTGAGTATTCCGATTGTGGTGATCGGAATCTATCCGGCGCTCTTCTTTGCAGGAAT
GCAGCACAGTGTAGCAGCGCTGGTAGCCGATTTAATGGCGCAGGTGGCCGGGCGATAA

Upstream 100 bases:

>100_bases
GCAGTGATGGTTATCGGCGGCACTGTGTATCCATAGTTGTCGCCGATAGCAGCACTCAGCTAACGCCAGCTCTGCGATAT
GACTCGATTGAGGGAGGATT

Downstream 100 bases:

>100_bases
GGCTGGCAGGTGTTCATGTCAGTAGCCCAGGCGAGGAGGAGAAAGAATGGATTCATTGACGATCCCGCCGGTGGATCTTA
CATTGATGGCGCCGCTCTTG

Product: proton-translocating NADH-quinone oxidoreductase subunit M

Products: NA

Alternate protein names: NADH dehydrogenase I, chain 13; NDH-1, chain 13 [H]

Number of amino acids: Translated: 525; Mature: 525

Protein sequence:

>525_residues
MNQLGFPLLSLILWLPTVGALVLLFVPRTNDELARRVSLVTMIIGFLLSLLLPLRFETNPMQIAAVGAPPVMQFVEELPW
LPIVGATYSLGIDGISLWLVMLTTFLGPIVVLSTWDSVHKDVRNFQILLLVLQTAMIGVFIAQDLLLFYLFWEFTLIPMT
FLIGIWGSQNRIYAARKFFLYTFAGSVLMLLALIALHILHRDAIAASDPNFRGTFSFSQFVSDMRAGRFTLDTVTERLLF
GAFFLAFAVKVPLWPFHTWLPDAHVEAPTAGSVVLAGVLLKLGGYGIIRYNLTLFPAAAQWAAPALAILAVIGIIYGAAV
AFAQSDMKRLVAYSSVSHMGFVVLGIFALNAEGISGAVLQMVNHGLSTSALFLMVGVLYERRHTRDLAEFGGLWKVMPVY
AAFSLLVALSSAGLPGLNGFVGEFTIITGAFRSPLLGWVYVAFAVGGVVLAAAYLLKLFRSVFMGEVSQPANASLPDLNR
RELTAFALLSIPIVVIGIYPALFFAGMQHSVAALVADLMAQVAGR

Sequences:

>Translated_525_residues
MNQLGFPLLSLILWLPTVGALVLLFVPRTNDELARRVSLVTMIIGFLLSLLLPLRFETNPMQIAAVGAPPVMQFVEELPW
LPIVGATYSLGIDGISLWLVMLTTFLGPIVVLSTWDSVHKDVRNFQILLLVLQTAMIGVFIAQDLLLFYLFWEFTLIPMT
FLIGIWGSQNRIYAARKFFLYTFAGSVLMLLALIALHILHRDAIAASDPNFRGTFSFSQFVSDMRAGRFTLDTVTERLLF
GAFFLAFAVKVPLWPFHTWLPDAHVEAPTAGSVVLAGVLLKLGGYGIIRYNLTLFPAAAQWAAPALAILAVIGIIYGAAV
AFAQSDMKRLVAYSSVSHMGFVVLGIFALNAEGISGAVLQMVNHGLSTSALFLMVGVLYERRHTRDLAEFGGLWKVMPVY
AAFSLLVALSSAGLPGLNGFVGEFTIITGAFRSPLLGWVYVAFAVGGVVLAAAYLLKLFRSVFMGEVSQPANASLPDLNR
RELTAFALLSIPIVVIGIYPALFFAGMQHSVAALVADLMAQVAGR
>Mature_525_residues
MNQLGFPLLSLILWLPTVGALVLLFVPRTNDELARRVSLVTMIIGFLLSLLLPLRFETNPMQIAAVGAPPVMQFVEELPW
LPIVGATYSLGIDGISLWLVMLTTFLGPIVVLSTWDSVHKDVRNFQILLLVLQTAMIGVFIAQDLLLFYLFWEFTLIPMT
FLIGIWGSQNRIYAARKFFLYTFAGSVLMLLALIALHILHRDAIAASDPNFRGTFSFSQFVSDMRAGRFTLDTVTERLLF
GAFFLAFAVKVPLWPFHTWLPDAHVEAPTAGSVVLAGVLLKLGGYGIIRYNLTLFPAAAQWAAPALAILAVIGIIYGAAV
AFAQSDMKRLVAYSSVSHMGFVVLGIFALNAEGISGAVLQMVNHGLSTSALFLMVGVLYERRHTRDLAEFGGLWKVMPVY
AAFSLLVALSSAGLPGLNGFVGEFTIITGAFRSPLLGWVYVAFAVGGVVLAAAYLLKLFRSVFMGEVSQPANASLPDLNR
RELTAFALLSIPIVVIGIYPALFFAGMQHSVAALVADLMAQVAGR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=376, Percent_Identity=35.9042553191489, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI251831117, Length=394, Percent_Identity=25.1269035532995, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1788613, Length=500, Percent_Identity=41.6, Blast_Score=341, Evalue=6e-95,
Organism=Escherichia coli, GI1788827, Length=307, Percent_Identity=28.3387622149837, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1788831, Length=294, Percent_Identity=30.952380952381, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1788829, Length=440, Percent_Identity=26.5909090909091, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI145693160, Length=399, Percent_Identity=27.3182957393484, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI2367154, Length=396, Percent_Identity=27.5252525252525, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788614, Length=387, Percent_Identity=26.6149870801034, Blast_Score=76, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918
- InterPro:   IPR000260 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01059 Oxidored_q5_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 57110; Mature: 57110

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8422400 [H]