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The map label for this gene is yfiJ [H]

Identifier: 222524001

GI number: 222524001

Start: 897845

End: 899083

Strand: Reverse

Name: yfiJ [H]

Synonym: Chy400_0716

Alternate gene names: 222524001

Gene position: 899083-897845 (Counterclockwise)

Preceding gene: 222524002

Following gene: 222524000

Centisome position: 17.06

GC content: 57.71

Gene sequence:

>1239_bases
ATGATGTCGTCAGCACAGCAAACCGGGTGGTGGCAACGGTTAACCGCCTATTTCAATAACGGTCAGCATACATTAATGAT
GGCAGCCTATGCCGTCATCACGGTAGCGCTGGTTGAGTATATCCTCTTTCATCGTCATCTACCGGCAGAACGCTTTTATC
CGGTCATAGTGCTCTTGAGTCTGCTGCTGGCCCTTAATGCGGCTTGGGATAGCCTGCAACTGCGCTGGGGTGATGTGGTC
GCCAATCGCCTCTTTTTCGGGCTGAGTTCATTGATCTTTCTCGTGGTCAATTATCTTGGTCTCGATGAAGGCTGGACCTT
CCTGCCCTTCATTCTCTTTGTGATTGGCTCGCAGGCCATCATCGGTCTCGGTATCTGGCCTGGTCTGGCGCTCAGCCTCT
TGCTCTATGCCGGCTGGAATGGCGTGCTCTGGCTGCGTGGCGTGTCGTCAACCCAAATCCTGACCCAGGCGCCATCGATT
GCGCTGGGTCTCATCTTCACCCTCATCTTTTCCATCGTGTTAGCCCGGCTCAGCGAACAGATGGCGCGCACTGAGCGTCT
GGCGGCTGAACTACGGGCCGCGAATGAGGCGTTAGCAGCTTCGCGCGAGCGCGAACTGGCCCTGGCTGCTGCCGAAGAAC
GGGTTCGCCTGGCGCACGAGATTCACGATGGCCTGGGCCATCATCTAACCGCCCTGCACGTGCAGTTACAGGTCGCAGCC
CGCTTACTCGAACACGATCCGGTTCGTGCTGCGCAGGCGCTTCATCTGTGCCGTGAAGCGGCACAGGCCGCCTTAACCGA
AGTGCGTCAGAGTGTGGCTATAATGCGGAGCAATCCACTTGCCGAGCAGCCGTTACCGGTCGTCATTGAACGGCTGGTAC
ACGATTTCAGCCGGGTCGCGTCCTTCCAGATCAGGTTTGAACAGGCGGGTGAGATTGGTGAGATTTCGCCGGCAATGGTC
ATGACGCTTTTCCGCGCTGCCCAAGAGGGTTTGACGAACGTGCAAAAGCATGCTCAGGCTACGTGTGTAACGGTGCGATT
GATTGCCGATACCGAATCGGTGCGTCTCGACGTGATCAATGACGGGCCGCCCGCTCCTGCGATTGGCGAAACCGGTTTTG
GTCTGGCCGGATTGCGCGAACGGGTGAGCCGGCTGGGTGGCGAGATACAGGCTGCACCGCAACCGGAAGGTGGTTTTCGC
CTTGCGATTGTGCTTCCCCGCAGATCGAGAAATGCATGA

Upstream 100 bases:

>100_bases
ATCATCTCTGGTGGCTCGACCATCTGCCGCGAGTTGCCGGTGAAACTGATGGCGTCCTCAACAACTGGTGGCATTACATT
TTACAGCCGTGGGTCGTGCA

Downstream 100 bases:

>100_bases
CAGAGTAAGGCGGAGAGCGCCCATGTGTATCGCTGAAAAACTCTTTGGCGTCAGGGGATCGCGAAGTGCGGAAGCCATAC
TTCCGCGTCACCCCGGTCTG

Product: integral membrane sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MMSSAQQTGWWQRLTAYFNNGQHTLMMAAYAVITVALVEYILFHRHLPAERFYPVIVLLSLLLALNAAWDSLQLRWGDVV
ANRLFFGLSSLIFLVVNYLGLDEGWTFLPFILFVIGSQAIIGLGIWPGLALSLLLYAGWNGVLWLRGVSSTQILTQAPSI
ALGLIFTLIFSIVLARLSEQMARTERLAAELRAANEALAASRERELALAAAEERVRLAHEIHDGLGHHLTALHVQLQVAA
RLLEHDPVRAAQALHLCREAAQAALTEVRQSVAIMRSNPLAEQPLPVVIERLVHDFSRVASFQIRFEQAGEIGEISPAMV
MTLFRAAQEGLTNVQKHAQATCVTVRLIADTESVRLDVINDGPPAPAIGETGFGLAGLRERVSRLGGEIQAAPQPEGGFR
LAIVLPRRSRNA

Sequences:

>Translated_412_residues
MMSSAQQTGWWQRLTAYFNNGQHTLMMAAYAVITVALVEYILFHRHLPAERFYPVIVLLSLLLALNAAWDSLQLRWGDVV
ANRLFFGLSSLIFLVVNYLGLDEGWTFLPFILFVIGSQAIIGLGIWPGLALSLLLYAGWNGVLWLRGVSSTQILTQAPSI
ALGLIFTLIFSIVLARLSEQMARTERLAAELRAANEALAASRERELALAAAEERVRLAHEIHDGLGHHLTALHVQLQVAA
RLLEHDPVRAAQALHLCREAAQAALTEVRQSVAIMRSNPLAEQPLPVVIERLVHDFSRVASFQIRFEQAGEIGEISPAMV
MTLFRAAQEGLTNVQKHAQATCVTVRLIADTESVRLDVINDGPPAPAIGETGFGLAGLRERVSRLGGEIQAAPQPEGGFR
LAIVLPRRSRNA
>Mature_412_residues
MMSSAQQTGWWQRLTAYFNNGQHTLMMAAYAVITVALVEYILFHRHLPAERFYPVIVLLSLLLALNAAWDSLQLRWGDVV
ANRLFFGLSSLIFLVVNYLGLDEGWTFLPFILFVIGSQAIIGLGIWPGLALSLLLYAGWNGVLWLRGVSSTQILTQAPSI
ALGLIFTLIFSIVLARLSEQMARTERLAAELRAANEALAASRERELALAAAEERVRLAHEIHDGLGHHLTALHVQLQVAA
RLLEHDPVRAAQALHLCREAAQAALTEVRQSVAIMRSNPLAEQPLPVVIERLVHDFSRVASFQIRFEQAGEIGEISPAMV
MTLFRAAQEGLTNVQKHAQATCVTVRLIADTESVRLDVINDGPPAPAIGETGFGLAGLRERVSRLGGEIQAAPQPEGGFR
LAIVLPRRSRNA

Specific function: Member of the two-component regulatory system yfiJ/yfiK. Probably activates yfiK by phosphorylation [H]

COG id: COG4585

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI87082311, Length=230, Percent_Identity=31.7391304347826, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787474, Length=218, Percent_Identity=24.7706422018349, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1788812, Length=192, Percent_Identity=27.6041666666667, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011712 [H]

Pfam domain/function: PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 45239; Mature: 45239

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSSAQQTGWWQRLTAYFNNGQHTLMMAAYAVITVALVEYILFHRHLPAERFYPVIVLLS
CCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LLLALNAAWDSLQLRWGDVVANRLFFGLSSLIFLVVNYLGLDEGWTFLPFILFVIGSQAI
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHH
IGLGIWPGLALSLLLYAGWNGVLWLRGVSSTQILTQAPSIALGLIFTLIFSIVLARLSEQ
EEHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
MARTERLAAELRAANEALAASRERELALAAAEERVRLAHEIHDGLGHHLTALHVQLQVAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLLEHDPVRAAQALHLCREAAQAALTEVRQSVAIMRSNPLAEQPLPVVIERLVHDFSRVA
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
SFQIRFEQAGEIGEISPAMVMTLFRAAQEGLTNVQKHAQATCVTVRLIADTESVRLDVIN
HHHEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCEEEEEEC
DGPPAPAIGETGFGLAGLRERVSRLGGEIQAAPQPEGGFRLAIVLPRRSRNA
CCCCCCCCCCCCCCHHHHHHHHHHCCCCEECCCCCCCCEEEEEEECCCCCCC
>Mature Secondary Structure
MMSSAQQTGWWQRLTAYFNNGQHTLMMAAYAVITVALVEYILFHRHLPAERFYPVIVLLS
CCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LLLALNAAWDSLQLRWGDVVANRLFFGLSSLIFLVVNYLGLDEGWTFLPFILFVIGSQAI
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHH
IGLGIWPGLALSLLLYAGWNGVLWLRGVSSTQILTQAPSIALGLIFTLIFSIVLARLSEQ
EEHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
MARTERLAAELRAANEALAASRERELALAAAEERVRLAHEIHDGLGHHLTALHVQLQVAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLLEHDPVRAAQALHLCREAAQAALTEVRQSVAIMRSNPLAEQPLPVVIERLVHDFSRVA
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
SFQIRFEQAGEIGEISPAMVMTLFRAAQEGLTNVQKHAQATCVTVRLIADTESVRLDVIN
HHHEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCEEEEEEC
DGPPAPAIGETGFGLAGLRERVSRLGGEIQAAPQPEGGFRLAIVLPRRSRNA
CCCCCCCCCCCCCCHHHHHHHHHHCCCCEECCCCCCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8973323; 9384377 [H]