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The map label for this gene is yfiK [H]

Identifier: 222524000

GI number: 222524000

Start: 896894

End: 897547

Strand: Reverse

Name: yfiK [H]

Synonym: Chy400_0715

Alternate gene names: 222524000

Gene position: 897547-896894 (Counterclockwise)

Preceding gene: 222524001

Following gene: 222523992

Centisome position: 17.03

GC content: 56.42

Gene sequence:

>654_bases
ATGATCCGCATCCTGCTGGTTGATGACCAGACCCTGGTACGTCAGGGTATTCAGACCCTGCTCGATCTTGAAGCAGATTT
GACAGTTGTCGGCACTGCCGTGAATGGTCAGCAAGCCATTGAACTCGTTGAGCGGTTGCAACCGGATGTTGTTCTCATGG
ATATACGGATGCCGGTAATGGATGGGGTTGCCGCCACTCGCGAGATTTGTCGGCGTTGGCCACACATCGGTGTGATCATC
CTGACCACCTTTGATGACGATGAATACGTGATTGAGGGCTTGAAAGCCGGTGCCCGTGGCTACATGCTCAAAGATGCCGA
CAGCAGCGACATTGTCGCCTCAATCCGCATTGTGGCCCGTGGTGAGGCATTGATTCAGCCGAGTATCACCCGCAAAGTAC
TGGCCGAATTCTCTCGCCTGGCCGCTTCGGCACCGGCCACCCGGCCATCGCCGTTAGCCGAACCGCTTACCGAGCGTGAG
ATGGATGTGTTGCATGGCATTGCTGCCGGACACTCAAACCGCGAGATTGCCGATCAGCTCTGTATCAGCGAGGGTACTGT
CAAAAACTACGTCTCTAGCCTGCTGGCCAAACTGGCCGTGCGTGACCGCACCCAGGCCATCATCCGTGCGCGTGAATTGG
GGTTGTTGCCGTAA

Upstream 100 bases:

>100_bases
CACATCTTTGCATCCGCCGCTGACGCGCTCGCAGATGCTGTGCAGTGTCCGGACAGCGTCACCTGATACGTGGCGCTATA
GTAACGTCAGGCGAGGGACT

Downstream 100 bases:

>100_bases
TTCCAGCGAGTGCTTGCTATCTATGCAGTCAGCACCCAGATATCTCTGATCAGAAATGTCTGCAAGTCTTTAATCTGCTC
GTAGAATTGCTTGCCGATCA

Product: LuxR family two component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MIRILLVDDQTLVRQGIQTLLDLEADLTVVGTAVNGQQAIELVERLQPDVVLMDIRMPVMDGVAATREICRRWPHIGVII
LTTFDDDEYVIEGLKAGARGYMLKDADSSDIVASIRIVARGEALIQPSITRKVLAEFSRLAASAPATRPSPLAEPLTERE
MDVLHGIAAGHSNREIADQLCISEGTVKNYVSSLLAKLAVRDRTQAIIRARELGLLP

Sequences:

>Translated_217_residues
MIRILLVDDQTLVRQGIQTLLDLEADLTVVGTAVNGQQAIELVERLQPDVVLMDIRMPVMDGVAATREICRRWPHIGVII
LTTFDDDEYVIEGLKAGARGYMLKDADSSDIVASIRIVARGEALIQPSITRKVLAEFSRLAASAPATRPSPLAEPLTERE
MDVLHGIAAGHSNREIADQLCISEGTVKNYVSSLLAKLAVRDRTQAIIRARELGLLP
>Mature_217_residues
MIRILLVDDQTLVRQGIQTLLDLEADLTVVGTAVNGQQAIELVERLQPDVVLMDIRMPVMDGVAATREICRRWPHIGVII
LTTFDDDEYVIEGLKAGARGYMLKDADSSDIVASIRIVARGEALIQPSITRKVLAEFSRLAASAPATRPSPLAEPLTERE
MDVLHGIAAGHSNREIADQLCISEGTVKNYVSSLLAKLAVRDRTQAIIRARELGLLP

Specific function: Member of the two-component regulatory system yfiJ/yfiK [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788521, Length=207, Percent_Identity=38.6473429951691, Blast_Score=121, Evalue=4e-29,
Organism=Escherichia coli, GI1788222, Length=201, Percent_Identity=35.3233830845771, Blast_Score=112, Evalue=1e-26,
Organism=Escherichia coli, GI1787473, Length=207, Percent_Identity=36.231884057971, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1790102, Length=203, Percent_Identity=31.5270935960591, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1788712, Length=198, Percent_Identity=27.2727272727273, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1786747, Length=208, Percent_Identity=25.4807692307692, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1788550, Length=105, Percent_Identity=40.9523809523809, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI87082052, Length=110, Percent_Identity=34.5454545454545, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1790299, Length=125, Percent_Identity=34.4, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI1790437, Length=114, Percent_Identity=37.719298245614, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23728; Mature: 23728

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRILLVDDQTLVRQGIQTLLDLEADLTVVGTAVNGQQAIELVERLQPDVVLMDIRMPVM
CEEEEEECCHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHCCCCEEEEEECCHHH
DGVAATREICRRWPHIGVIILTTFDDDEYVIEGLKAGARGYMLKDADSSDIVASIRIVAR
HHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHCCCCCCEEECCCCCHHHEEEEEEEC
GEALIQPSITRKVLAEFSRLAASAPATRPSPLAEPLTEREMDVLHGIAAGHSNREIADQL
CCHHHCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
CISEGTVKNYVSSLLAKLAVRDRTQAIIRARELGLLP
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIRILLVDDQTLVRQGIQTLLDLEADLTVVGTAVNGQQAIELVERLQPDVVLMDIRMPVM
CEEEEEECCHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHCCCCEEEEEECCHHH
DGVAATREICRRWPHIGVIILTTFDDDEYVIEGLKAGARGYMLKDADSSDIVASIRIVAR
HHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHCCCCCCEEECCCCCHHHEEEEEEEC
GEALIQPSITRKVLAEFSRLAASAPATRPSPLAEPLTEREMDVLHGIAAGHSNREIADQL
CCHHHCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
CISEGTVKNYVSSLLAKLAVRDRTQAIIRARELGLLP
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8973323; 9384377 [H]