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The map label for this gene is rph [H]

Identifier: 222523554

GI number: 222523554

Start: 312206

End: 312958

Strand: Direct

Name: rph [H]

Synonym: Chy400_0260

Alternate gene names: 222523554

Gene position: 312206-312958 (Clockwise)

Preceding gene: 222523553

Following gene: 222523555

Centisome position: 5.93

GC content: 58.83

Gene sequence:

>753_bases
ATGATGCGGATTGATGGACGGTCACCAGACCAACTACGTCCCATTTCCATCGAGATCGGTGCGGCACCGTATGCAGAGGG
ATCGGCACTGATCGCCTACGGAAAGACTCGTGTTCTCTGCACCGCTACGGTGGAAGATGGAGTACCAGCCTGGATGCGCG
GCCAGCAGAGTGGCTGGGTCACTGCCGAGTATGCAATGCTGCCACGTGCCACTCTCCAACGCACCCGTCGCGAACGCAAC
GGGCCGAGTGGCCGAACCCAAGAAATTCAGCGGCTGATCGGTCGTTCGTTGCGGGCTGCGATTGACCTCAATGCGCTTGG
GGAACGTACTATCACCATCGATTGTGATGTGTTACAGGCCGATGGCGGTACCCGCACGGCAGCAATCAGTGGCGGTTACG
TTGCCCTGGCGCTGGCGGTAGATTACCTGGTACGCAGCGGCGTCCTGGAAACAAGACCAACGCTGACACCGGTGGCTGCT
GTAAGTGTGGGCATGCTTCAGGGCGTGTTACTGCTTGATCTCTGCTACGAAGAAGACAGTCAGGCCGACCTCGATTGCAA
CGTAGTGATGAACAGTGCCGGCGGGTATATCGAAGTCCAGGCCACAGCCGAGCGATCAGCAGTCACTCGCGACCAGCTCA
ACGCACTGCTCGATCTGGCCGGACGAGGCATTCAACAGATTTTGGACGCACAGGCCGCTGCGCTGGCAACTGCCTTGCCC
AACAACGCCATGAGCCAGGTACAATCCCCATAA

Upstream 100 bases:

>100_bases
GGCAGGCACGGCGGATTATGATCAGTTAAGTAATCTCACGTTGTATCGTGGTCACAAACGTGGTATGATGGTCAATAAGA
GGTAGAGGAAACGGTTTCGT

Downstream 100 bases:

>100_bases
CCCGGATTGTACCCGATCAACCTGCGACGTCATGGCTCGCAACGGTCGCCCTCCATGCCGGGCGAGGAGGTTTATCATGA
CAGACGAGACCAGACGCGTT

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase [H]

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MMRIDGRSPDQLRPISIEIGAAPYAEGSALIAYGKTRVLCTATVEDGVPAWMRGQQSGWVTAEYAMLPRATLQRTRRERN
GPSGRTQEIQRLIGRSLRAAIDLNALGERTITIDCDVLQADGGTRTAAISGGYVALALAVDYLVRSGVLETRPTLTPVAA
VSVGMLQGVLLLDLCYEEDSQADLDCNVVMNSAGGYIEVQATAERSAVTRDQLNALLDLAGRGIQQILDAQAAALATALP
NNAMSQVQSP

Sequences:

>Translated_250_residues
MMRIDGRSPDQLRPISIEIGAAPYAEGSALIAYGKTRVLCTATVEDGVPAWMRGQQSGWVTAEYAMLPRATLQRTRRERN
GPSGRTQEIQRLIGRSLRAAIDLNALGERTITIDCDVLQADGGTRTAAISGGYVALALAVDYLVRSGVLETRPTLTPVAA
VSVGMLQGVLLLDLCYEEDSQADLDCNVVMNSAGGYIEVQATAERSAVTRDQLNALLDLAGRGIQQILDAQAAALATALP
NNAMSQVQSP
>Mature_250_residues
MMRIDGRSPDQLRPISIEIGAAPYAEGSALIAYGKTRVLCTATVEDGVPAWMRGQQSGWVTAEYAMLPRATLQRTRRERN
GPSGRTQEIQRLIGRSLRAAIDLNALGERTITIDCDVLQADGGTRTAAISGGYVALALAVDYLVRSGVLETRPTLTPVAA
VSVGMLQGVLLLDLCYEEDSQADLDCNVVMNSAGGYIEVQATAERSAVTRDQLNALLDLAGRGIQQILDAQAAALATALP
NNAMSQVQSP

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates [H]

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family [H]

Homologues:

Organism=Escherichia coli, GI157672248, Length=211, Percent_Identity=55.9241706161137, Blast_Score=226, Evalue=8e-61,
Organism=Caenorhabditis elegans, GI71981632, Length=219, Percent_Identity=27.3972602739726, Blast_Score=74, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336 [H]

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C [H]

EC number: =2.7.7.56 [H]

Molecular weight: Translated: 26586; Mature: 26586

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRIDGRSPDQLRPISIEIGAAPYAEGSALIAYGKTRVLCTATVEDGVPAWMRGQQSGWV
CCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEECCEEEEEEEECCCCCCHHHCCCCCCCE
TAEYAMLPRATLQRTRRERNGPSGRTQEIQRLIGRSLRAAIDLNALGERTITIDCDVLQA
EEHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEC
DGGTRTAAISGGYVALALAVDYLVRSGVLETRPTLTPVAAVSVGMLQGVLLLDLCYEEDS
CCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
QADLDCNVVMNSAGGYIEVQATAERSAVTRDQLNALLDLAGRGIQQILDAQAAALATALP
CCCCEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NNAMSQVQSP
CHHHHHCCCC
>Mature Secondary Structure
MMRIDGRSPDQLRPISIEIGAAPYAEGSALIAYGKTRVLCTATVEDGVPAWMRGQQSGWV
CCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEECCEEEEEEEECCCCCCHHHCCCCCCCE
TAEYAMLPRATLQRTRRERNGPSGRTQEIQRLIGRSLRAAIDLNALGERTITIDCDVLQA
EEHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEC
DGGTRTAAISGGYVALALAVDYLVRSGVLETRPTLTPVAAVSVGMLQGVLLLDLCYEEDS
CCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
QADLDCNVVMNSAGGYIEVQATAERSAVTRDQLNALLDLAGRGIQQILDAQAAALATALP
CCCCEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NNAMSQVQSP
CHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA