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The map label for this gene is ligA

Identifier: 222523550

GI number: 222523550

Start: 305115

End: 307244

Strand: Direct

Name: ligA

Synonym: Chy400_0256

Alternate gene names: 222523550

Gene position: 305115-307244 (Clockwise)

Preceding gene: 222523549

Following gene: 222523553

Centisome position: 5.79

GC content: 59.06

Gene sequence:

>2130_bases
ATGAGCCATACGACTGTCGCTGATCGCATCAACGAGCTACGCAGCCTGATCCGCCGCTACGACTATCACTACTACGTGCT
CGACGATCCCATCGTCAGCGATGCTGAATACGATGCGCTGATGACTGAATTGCGCGCATTGGAGGCGGCACACCCAGAGT
TAATCACCCCCGACTCGCCAACCCAACGGGTAAGCGGGACACCTGCATCTCAGTTTGCCAAAGTACAACACCCGCAGCCG
ATGTTATCACTCGGTAATGCCTTTACGAAGGCTGATCTCCTGGCATGGCGGGATCGGGTACTACGACTCCTTGGCCCCGA
TGCCATAGTAGCCTATGTGGTCGAGCCAAAGATTGACGGTCTGGCCGTTGCTCTGACCTACCGCGATGGCCGCTTGGTGC
AGGGGGCCACCCGTGGCGACGGTGAGGTTGGCGAAGACGTGACGGCAAATCTGCGCACCATCGGCAGTATCCCGCTGACA
CTTCAAGCGACCAGTACGCCCCAAGATGATGACTTGCCGACGACATTGCCGACCACGATAGAGGTGCGGGGCGAAGTCTA
CATGCGCACGGCTGATTTTGAGACGCTGAATGATCGGCTTGCCGCCGCAGGAGAGAAAATCTTCGCGAACCCACGCAACG
CTGCTGCCGGCTCGTTACGCCAGAAAGACCCCACAATTACCGCGGCCCGTCCGCTCCGCTTCTTTGCCTACGGTGTTGGC
GTCGTTGAAGGCATTTCGCTCAGCAGTCAATGGCAAACGCTCCGCTATCTGCGCGCACTCGGCTTTCCAGTCAACCAGGA
TGTTCGCCGCTTCACCGACTTCGCTGAAGTCCTGGCCTATTGCGAAGCCTGGATGGCCAAACGTGACGATCTACCTTACG
AAGCCGATGGGGTAGTGATCAAGATCGATGATTTCGCCCAGCAACGCGAACTGGGTGTTGTCGGGCGCGATCCCCGTTGG
GCAATTGCCTTCAAATTCCCGGCCCGCGAAGCGATCACCCGCTTGCTCGATATTACGGTCAATGTTGGTCGTACCGGCGT
CGTCACACCCAACGCTGAACTGGAGCCGGTACAGATCGGCGGTGTGACAGTACGAAACGCCAGTTTGCACAACGCCGATT
ACATTGCCCAGCGCGACATTCGGATCGGCGATTATGTCATCGTCAAACGCGCCGGTGACGTGATACCCTACGTGGTCGGC
CCGGTGATCGCCCGTCGCGATGGCAGTGAGCGGCCCTGGCAATTTCCAACCCATTGCCCGGCCTGTGGCTCACCGCTTGA
ACGCGAAGAAGGTGAGGCGGCCTGGCGCTGCAACAATTTCAGCATCTGCCCGGCCCAACTGGTACGCCGGGTCGAACACT
TCGTCAGCCGATCAGCCCTCGACATTGTTGGGATGGGTGAGCGACAGGCAGAACTCTTCGTCCAGCGCGGTCTCATTCGT
GATGTAGCAGACATTTTCTTCTTGAAAGCCGATCAACTGGCAGAACTGGAGGGATTCGGCCCGAAACGGATCGCCAACCT
GCTCGCCGCCATTGACGCTGCCCGGCAACGGCCACTCGACCGGCTCCTGGTCGGGCTGGGTATCCGCTACGTCGGCACCG
TCGCCGCCCAAACTCTTGTTGCGGCGCTGGGATCACTCGACGCAATCATGGCAGCGCGCCAGGAAGAACTGGAACAGATT
CCCGGTATTGGCCCGGTGGTAGCGGCCAGTATTGTTGATTTCTTCTCACGTCCAGCAAACCGGGCGCTGATAGAGAAGCT
GCGGGCAGCCGGGGTACAGATGGGTGGGGTGAGTGGTCCAACGCGCCAGAGCGACACCCTCGCCGGCAAGACATTTGTCT
TGACCGGAACGCTACCCTCGCTGAGCCGTGAGCAAGCCAGCGCACTCATTGTTGCCCATGGCGGTAAGGTGACCGATAGT
GTGAGCAAAAAGACCAGCTATGTGGTGGCCGGAGCTAATGCCGGGAGTAAGCTCGCCAAAGCACTCCAGTTAGGGATTCC
GGTCATTGACGAAGCCGGTCTGCTGGCATTGATCGGAACAACGGCAGAACCACCACCATCGCCACCACCACCTCCACCGG
AAACCAACACCGACGGCAATCAGTTGCTCTTACCGCTTGACGGAGAATGA

Upstream 100 bases:

>100_bases
AACGGTTCTTTTACTTTTTGGCAACCCCCCAACGGTACTACTTTAGACCATTTTTTTATAGTACCTCCGACGAACTTCTA
CCGTAACCGTGGAGTTTCCA

Downstream 100 bases:

>100_bases
CCCGACGGCGTGGCAGTGCTCCGCTCCAGCCGTGCCGGCACGTGCCGGATGGGTTGCCTTACACACGCATCATGTGCGTG
GCGCAGGGTTTGGAGTGCGG

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 709; Mature: 708

Protein sequence:

>709_residues
MSHTTVADRINELRSLIRRYDYHYYVLDDPIVSDAEYDALMTELRALEAAHPELITPDSPTQRVSGTPASQFAKVQHPQP
MLSLGNAFTKADLLAWRDRVLRLLGPDAIVAYVVEPKIDGLAVALTYRDGRLVQGATRGDGEVGEDVTANLRTIGSIPLT
LQATSTPQDDDLPTTLPTTIEVRGEVYMRTADFETLNDRLAAAGEKIFANPRNAAAGSLRQKDPTITAARPLRFFAYGVG
VVEGISLSSQWQTLRYLRALGFPVNQDVRRFTDFAEVLAYCEAWMAKRDDLPYEADGVVIKIDDFAQQRELGVVGRDPRW
AIAFKFPAREAITRLLDITVNVGRTGVVTPNAELEPVQIGGVTVRNASLHNADYIAQRDIRIGDYVIVKRAGDVIPYVVG
PVIARRDGSERPWQFPTHCPACGSPLEREEGEAAWRCNNFSICPAQLVRRVEHFVSRSALDIVGMGERQAELFVQRGLIR
DVADIFFLKADQLAELEGFGPKRIANLLAAIDAARQRPLDRLLVGLGIRYVGTVAAQTLVAALGSLDAIMAARQEELEQI
PGIGPVVAASIVDFFSRPANRALIEKLRAAGVQMGGVSGPTRQSDTLAGKTFVLTGTLPSLSREQASALIVAHGGKVTDS
VSKKTSYVVAGANAGSKLAKALQLGIPVIDEAGLLALIGTTAEPPPSPPPPPPETNTDGNQLLLPLDGE

Sequences:

>Translated_709_residues
MSHTTVADRINELRSLIRRYDYHYYVLDDPIVSDAEYDALMTELRALEAAHPELITPDSPTQRVSGTPASQFAKVQHPQP
MLSLGNAFTKADLLAWRDRVLRLLGPDAIVAYVVEPKIDGLAVALTYRDGRLVQGATRGDGEVGEDVTANLRTIGSIPLT
LQATSTPQDDDLPTTLPTTIEVRGEVYMRTADFETLNDRLAAAGEKIFANPRNAAAGSLRQKDPTITAARPLRFFAYGVG
VVEGISLSSQWQTLRYLRALGFPVNQDVRRFTDFAEVLAYCEAWMAKRDDLPYEADGVVIKIDDFAQQRELGVVGRDPRW
AIAFKFPAREAITRLLDITVNVGRTGVVTPNAELEPVQIGGVTVRNASLHNADYIAQRDIRIGDYVIVKRAGDVIPYVVG
PVIARRDGSERPWQFPTHCPACGSPLEREEGEAAWRCNNFSICPAQLVRRVEHFVSRSALDIVGMGERQAELFVQRGLIR
DVADIFFLKADQLAELEGFGPKRIANLLAAIDAARQRPLDRLLVGLGIRYVGTVAAQTLVAALGSLDAIMAARQEELEQI
PGIGPVVAASIVDFFSRPANRALIEKLRAAGVQMGGVSGPTRQSDTLAGKTFVLTGTLPSLSREQASALIVAHGGKVTDS
VSKKTSYVVAGANAGSKLAKALQLGIPVIDEAGLLALIGTTAEPPPSPPPPPPETNTDGNQLLLPLDGE
>Mature_708_residues
SHTTVADRINELRSLIRRYDYHYYVLDDPIVSDAEYDALMTELRALEAAHPELITPDSPTQRVSGTPASQFAKVQHPQPM
LSLGNAFTKADLLAWRDRVLRLLGPDAIVAYVVEPKIDGLAVALTYRDGRLVQGATRGDGEVGEDVTANLRTIGSIPLTL
QATSTPQDDDLPTTLPTTIEVRGEVYMRTADFETLNDRLAAAGEKIFANPRNAAAGSLRQKDPTITAARPLRFFAYGVGV
VEGISLSSQWQTLRYLRALGFPVNQDVRRFTDFAEVLAYCEAWMAKRDDLPYEADGVVIKIDDFAQQRELGVVGRDPRWA
IAFKFPAREAITRLLDITVNVGRTGVVTPNAELEPVQIGGVTVRNASLHNADYIAQRDIRIGDYVIVKRAGDVIPYVVGP
VIARRDGSERPWQFPTHCPACGSPLEREEGEAAWRCNNFSICPAQLVRRVEHFVSRSALDIVGMGERQAELFVQRGLIRD
VADIFFLKADQLAELEGFGPKRIANLLAAIDAARQRPLDRLLVGLGIRYVGTVAAQTLVAALGSLDAIMAARQEELEQIP
GIGPVVAASIVDFFSRPANRALIEKLRAAGVQMGGVSGPTRQSDTLAGKTFVLTGTLPSLSREQASALIVAHGGKVTDSV
SKKTSYVVAGANAGSKLAKALQLGIPVIDEAGLLALIGTTAEPPPSPPPPPPETNTDGNQLLLPLDGE

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Homo sapiens, GI32528306, Length=88, Percent_Identity=48.8636363636364, Blast_Score=76, Evalue=1e-13,
Organism=Escherichia coli, GI1788750, Length=679, Percent_Identity=48.159057437408, Blast_Score=612, Evalue=1e-176,
Organism=Escherichia coli, GI87082305, Length=587, Percent_Identity=24.3611584327087, Blast_Score=115, Evalue=1e-26,
Organism=Drosophila melanogaster, GI62472391, Length=94, Percent_Identity=44.6808510638298, Blast_Score=73, Evalue=7e-13,
Organism=Drosophila melanogaster, GI17737765, Length=94, Percent_Identity=44.6808510638298, Blast_Score=73, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_CHLAA (A9WCA1)

Other databases:

- EMBL:   CP000909
- RefSeq:   YP_001633883.1
- ProteinModelPortal:   A9WCA1
- GeneID:   5827870
- GenomeReviews:   CP000909_GR
- KEGG:   cau:Caur_0241
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   CLSK975376
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; PF00633 HHH; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 76791; Mature: 76660

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 117-117 BINDING 115-115 BINDING 138-138 BINDING 185-185 BINDING 301-301 BINDING 325-325

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHTTVADRINELRSLIRRYDYHYYVLDDPIVSDAEYDALMTELRALEAAHPELITPDSP
CCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
TQRVSGTPASQFAKVQHPQPMLSLGNAFTKADLLAWRDRVLRLLGPDAIVAYVVEPKIDG
CCCCCCCCHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCC
LAVALTYRDGRLVQGATRGDGEVGEDVTANLRTIGSIPLTLQATSTPQDDDLPTTLPTTI
EEEEEEECCCEEEECCCCCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCEE
EVRGEVYMRTADFETLNDRLAAAGEKIFANPRNAAAGSLRQKDPTITAARPLRFFAYGVG
EECCEEEEEECCHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCCCCEEHHCHHHHHHHHHH
VVEGISLSSQWQTLRYLRALGFPVNQDVRRFTDFAEVLAYCEAWMAKRDDLPYEADGVVI
HHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
KIDDFAQQRELGVVGRDPRWAIAFKFPAREAITRLLDITVNVGRTGVVTPNAELEPVQIG
EECCCHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHEEECCCCEEECCCCCCCEEEEC
GVTVRNASLHNADYIAQRDIRIGDYVIVKRAGDVIPYVVGPVIARRDGSERPWQFPTHCP
CEEEECCCCCCCCHHEECCCEECCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCC
ACGSPLEREEGEAAWRCNNFSICPAQLVRRVEHFVSRSALDIVGMGERQAELFVQRGLIR
CCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCHHHEECCCCHHHHHHHHHHHHH
DVADIFFLKADQLAELEGFGPKRIANLLAAIDAARQRPLDRLLVGLGIRYVGTVAAQTLV
HHHHHHHHCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
AALGSLDAIMAARQEELEQIPGIGPVVAASIVDFFSRPANRALIEKLRAAGVQMGGVSGP
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCEECCCCCC
TRQSDTLAGKTFVLTGTLPSLSREQASALIVAHGGKVTDSVSKKTSYVVAGANAGSKLAK
CCCCCCCCCCEEEEECCCCCCCHHCCCEEEEECCCCCCCCHHCCCEEEEEECCCHHHHHH
ALQLGIPVIDEAGLLALIGTTAEPPPSPPPPPPETNTDGNQLLLPLDGE
HHHHCCCEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCC
>Mature Secondary Structure 
SHTTVADRINELRSLIRRYDYHYYVLDDPIVSDAEYDALMTELRALEAAHPELITPDSP
CCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
TQRVSGTPASQFAKVQHPQPMLSLGNAFTKADLLAWRDRVLRLLGPDAIVAYVVEPKIDG
CCCCCCCCHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCC
LAVALTYRDGRLVQGATRGDGEVGEDVTANLRTIGSIPLTLQATSTPQDDDLPTTLPTTI
EEEEEEECCCEEEECCCCCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCEE
EVRGEVYMRTADFETLNDRLAAAGEKIFANPRNAAAGSLRQKDPTITAARPLRFFAYGVG
EECCEEEEEECCHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCCCCEEHHCHHHHHHHHHH
VVEGISLSSQWQTLRYLRALGFPVNQDVRRFTDFAEVLAYCEAWMAKRDDLPYEADGVVI
HHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
KIDDFAQQRELGVVGRDPRWAIAFKFPAREAITRLLDITVNVGRTGVVTPNAELEPVQIG
EECCCHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHEEECCCCEEECCCCCCCEEEEC
GVTVRNASLHNADYIAQRDIRIGDYVIVKRAGDVIPYVVGPVIARRDGSERPWQFPTHCP
CEEEECCCCCCCCHHEECCCEECCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCC
ACGSPLEREEGEAAWRCNNFSICPAQLVRRVEHFVSRSALDIVGMGERQAELFVQRGLIR
CCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCHHHEECCCCHHHHHHHHHHHHH
DVADIFFLKADQLAELEGFGPKRIANLLAAIDAARQRPLDRLLVGLGIRYVGTVAAQTLV
HHHHHHHHCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
AALGSLDAIMAARQEELEQIPGIGPVVAASIVDFFSRPANRALIEKLRAAGVQMGGVSGP
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCEECCCCCC
TRQSDTLAGKTFVLTGTLPSLSREQASALIVAHGGKVTDSVSKKTSYVVAGANAGSKLAK
CCCCCCCCCCEEEEECCCCCCCHHCCCEEEEECCCCCCCCHHCCCEEEEEECCCHHHHHH
ALQLGIPVIDEAGLLALIGTTAEPPPSPPPPPPETNTDGNQLLLPLDGE
HHHHCCCEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA