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The map label for this gene is leuD

Identifier: 222523475

GI number: 222523475

Start: 209342

End: 209947

Strand: Direct

Name: leuD

Synonym: Chy400_0181

Alternate gene names: 222523475

Gene position: 209342-209947 (Clockwise)

Preceding gene: 222523474

Following gene: 222523478

Centisome position: 3.97

GC content: 56.93

Gene sequence:

>606_bases
GTGGAACCAGTTTCAACCATTACCGGCAAAGCCGTCGTGTTACCGGTAGAGAATATTGATACCGATCAGATCATCCCAGC
CCGCTTTCTCAAAGTGACCGACCGCAGCGGTCTGGCTGCCGGTCTCTTCGAGGCGTGGCGCTACCAGGCGGACGGCACAC
CCAATCCCGATTTTCCGCTGAACCGACCGGAAGCTGCCGGGGCAACCATTCTGATCAGTGGGCGTAATTTTGGCTGTGGC
AGCTCGCGCGAGCACGCCCCCTGGGCATTGCAGGACTACGGATTCAAAGCCGTGCTGGCACCATCGTTTGCCGACATCTT
TCGCAGTAATTCGCTCAAGATTGGCTTGCTGCCGGTAACGATTGACCAGGCGGTCTACGATGAACTGGTTGCCCGTTATG
CGGCTGATCCGCAGATGCATCTGACGATTGATCTGGCAACACAGACGGTGACATTACCGGATGGCCGACAGGTGCATTTT
CCGATTGATGCCTTCAGCAAATACTGCCTGCTGCATGGGGTCGATCAACTCGGCTTCTTGCTCCAACAGGAAGAGGCGAT
TATCGCCTACGAAGCCAGCCATCCGCAGCCGGTGACGACTCGCTGA

Upstream 100 bases:

>100_bases
GAGCACGCACGATGCTGGCCAGTCCACTCACCGCAGCAGCGGCAGCCATCACAGGCGTGGTTACCGATCCGCGCACGTTG
TTGAACTAGAAGAGGCTCTC

Downstream 100 bases:

>100_bases
GCACGAGGAGAGGGCACGACGTATATCAAGTCTCGGCTGATAGTGCGCATCATTCTGGCTATCGGAACTGTGCTGGCGCA
CCACCGGGTGCGACACCGGC

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MEPVSTITGKAVVLPVENIDTDQIIPARFLKVTDRSGLAAGLFEAWRYQADGTPNPDFPLNRPEAAGATILISGRNFGCG
SSREHAPWALQDYGFKAVLAPSFADIFRSNSLKIGLLPVTIDQAVYDELVARYAADPQMHLTIDLATQTVTLPDGRQVHF
PIDAFSKYCLLHGVDQLGFLLQQEEAIIAYEASHPQPVTTR

Sequences:

>Translated_201_residues
MEPVSTITGKAVVLPVENIDTDQIIPARFLKVTDRSGLAAGLFEAWRYQADGTPNPDFPLNRPEAAGATILISGRNFGCG
SSREHAPWALQDYGFKAVLAPSFADIFRSNSLKIGLLPVTIDQAVYDELVARYAADPQMHLTIDLATQTVTLPDGRQVHF
PIDAFSKYCLLHGVDQLGFLLQQEEAIIAYEASHPQPVTTR
>Mature_201_residues
MEPVSTITGKAVVLPVENIDTDQIIPARFLKVTDRSGLAAGLFEAWRYQADGTPNPDFPLNRPEAAGATILISGRNFGCG
SSREHAPWALQDYGFKAVLAPSFADIFRSNSLKIGLLPVTIDQAVYDELVARYAADPQMHLTIDLATQTVTLPDGRQVHF
PIDAFSKYCLLHGVDQLGFLLQQEEAIIAYEASHPQPVTTR

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=190, Percent_Identity=51.0526315789474, Blast_Score=196, Evalue=8e-52,
Organism=Saccharomyces cerevisiae, GI6321429, Length=199, Percent_Identity=45.2261306532663, Blast_Score=174, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_CHLAA (A9WC30)

Other databases:

- EMBL:   CP000909
- RefSeq:   YP_001633812.1
- ProteinModelPortal:   A9WC30
- SMR:   A9WC30
- GeneID:   5824743
- GenomeReviews:   CP000909_GR
- KEGG:   cau:Caur_0169
- HOGENOM:   HBG304838
- OMA:   DEISITM
- ProtClustDB:   PRK01641
- HAMAP:   MF_01031
- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR00171

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 21993; Mature: 21993

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPVSTITGKAVVLPVENIDTDQIIPARFLKVTDRSGLAAGLFEAWRYQADGTPNPDFPL
CCCCCCCCCCEEEEECCCCCCCCCCHHHHEEEECCCCCHHHHHHHHEECCCCCCCCCCCC
NRPEAAGATILISGRNFGCGSSREHAPWALQDYGFKAVLAPSFADIFRSNSLKIGLLPVT
CCCCCCCEEEEEECCCCCCCCCCCCCCEEHHHCCEEEEECCCHHHHHCCCCEEEEEEEEE
IDQAVYDELVARYAADPQMHLTIDLATQTVTLPDGRQVHFPIDAFSKYCLLHGVDQLGFL
HHHHHHHHHHHHHCCCCCEEEEEEEEEEEEECCCCCEEEEEHHHHHHHHHHHCHHHHHHH
LQQEEAIIAYEASHPQPVTTR
HCCCCEEEEEECCCCCCCCCC
>Mature Secondary Structure
MEPVSTITGKAVVLPVENIDTDQIIPARFLKVTDRSGLAAGLFEAWRYQADGTPNPDFPL
CCCCCCCCCCEEEEECCCCCCCCCCHHHHEEEECCCCCHHHHHHHHEECCCCCCCCCCCC
NRPEAAGATILISGRNFGCGSSREHAPWALQDYGFKAVLAPSFADIFRSNSLKIGLLPVT
CCCCCCCEEEEEECCCCCCCCCCCCCCEEHHHCCEEEEECCCHHHHHCCCCEEEEEEEEE
IDQAVYDELVARYAADPQMHLTIDLATQTVTLPDGRQVHFPIDAFSKYCLLHGVDQLGFL
HHHHHHHHHHHHHCCCCCEEEEEEEEEEEEECCCCCEEEEEHHHHHHHHHHHCHHHHHHH
LQQEEAIIAYEASHPQPVTTR
HCCCCEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA