Click here to switch to the map view.

The map label for this gene is leuC [H]

Identifier: 222523474

GI number: 222523474

Start: 207906

End: 209330

Strand: Direct

Name: leuC [H]

Synonym: Chy400_0180

Alternate gene names: 222523474

Gene position: 207906-209330 (Clockwise)

Preceding gene: 222523473

Following gene: 222523475

Centisome position: 3.95

GC content: 59.51

Gene sequence:

>1425_bases
ATGAACAAACCGCGCACCCTGTTTGAGAAAGTCTGGGAAGCACATCTGGTACGACCAGAAACCGCTGAAACGCCTGCCGT
GCTCTACATTGATCTGCACCTCATTCACGAGGTAACCTCACCGCAGGCGTTTACTGAATTACGCCAGCGCGGGCTGCGGG
TTCGCCGACCCGACAAGACGCTGGCGACGATGGATCACAGCACACCGACAACGCCGCGTAATCATCTGGGCATTATTCCC
GTCGTTGATCCAATGGCAATCAGCCAGCTTGAACAATTACGAAAAAACTGCGCCGAGTTTGGGATTCCACTGTTTGAATT
GGGTGACGAGAATCAGGGCATTGTGCACGTCATTGGGCCAGAGCAAGGATTGACGCAACCGGGGATGACCATCGTTTGTG
GTGATAGTCACACCAGCACGCACGGCGCTTTTGGTGCGTTGGCCTTCGGCATTGGCACCTCGGAAGTCGGCCATGTGCTG
GCAACCCAATGCCTGCTCCAACGCAAGCCAAAGACGTGTGCGGTACGGATTGATGGCCGGCTGGGGCCAGGGGTCACTGC
CAAGGACATCATTCTGGCGTTGATCGCCAAATACGGCGTCGGCGGCGGTACGGGGTACGTCTTTGAGTACATGGGTGAAG
CGATCCGTGCGCTGTCGATGGAAGAGCGGATGACGATCTGCAATATGAGCATCGAGGGTGGGGCACGGGCCGGTATGGTC
GCACCCGACGATACGACCTTCGAGTATATCGCCGGTAGGCCCTTTGCGCCGAAGGGGGCCGATTTCGAGGCCGCTGTGGC
ACGCTGGCGAACACTCCCCAGTGACGAGGGGGCAACCTTCGATCACGAGTTAACCCTCTCGGCCAGTGAACTGAAACCAA
TGATTACCTACGGTACCAACCCGGGTATGGGCATTCCCATTGACGCCCCAGTTCCACGCCCGGAGGATATGCCCGATGCA
CGGAGCCGGGCGGCACTCGACAAGGCGCTGGCGTACATGGGGCTTGAACCGGGCAAACCGCTCCTGGGCCACCCGGTTGA
TGTTGTCTTCATCGGTTCCTGCACCAACTCGCGCCTGTCAGATTTGCGGCAGGCAGCCCAGTTTTTCCGTGGACGCAAGG
TAGCCCCCGGCGTGCGAGTAATGGTGGTTCCCGGATCGCAGCAGGTCAAGCGCGCTGCCGAGGCCGAAGGGTTAGATCGC
ATCTTCAAAGAAGCCGGTGCCGAATGGCGTGAAGCCGGTTGCAGCGCCTGTCTGGGCATGAACGACGACAAAGTGCCACC
GGGTAAATACGCTGTCAGCACCTCGAACCGCAACTTTGAGGGTCGGCAGGGGCCGGGAGCACGCACGATGCTGGCCAGTC
CACTCACCGCAGCAGCGGCAGCCATCACAGGCGTGGTTACCGATCCGCGCACGTTGTTGAACTAG

Upstream 100 bases:

>100_bases
CCTGCATACGGGCATGCTGAAAATCTTTGGTAGCTATCCGCGCTGGCGTGGCGCTAATGGTAATCATGCGTCGTAGGTTG
CGGCAAGGAGGAGAGCAAAG

Downstream 100 bases:

>100_bases
AAGAGGCTCTCGTGGAACCAGTTTCAACCATTACCGGCAAAGCCGTCGTGTTACCGGTAGAGAATATTGATACCGATCAG
ATCATCCCAGCCCGCTTTCT

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MNKPRTLFEKVWEAHLVRPETAETPAVLYIDLHLIHEVTSPQAFTELRQRGLRVRRPDKTLATMDHSTPTTPRNHLGIIP
VVDPMAISQLEQLRKNCAEFGIPLFELGDENQGIVHVIGPEQGLTQPGMTIVCGDSHTSTHGAFGALAFGIGTSEVGHVL
ATQCLLQRKPKTCAVRIDGRLGPGVTAKDIILALIAKYGVGGGTGYVFEYMGEAIRALSMEERMTICNMSIEGGARAGMV
APDDTTFEYIAGRPFAPKGADFEAAVARWRTLPSDEGATFDHELTLSASELKPMITYGTNPGMGIPIDAPVPRPEDMPDA
RSRAALDKALAYMGLEPGKPLLGHPVDVVFIGSCTNSRLSDLRQAAQFFRGRKVAPGVRVMVVPGSQQVKRAAEAEGLDR
IFKEAGAEWREAGCSACLGMNDDKVPPGKYAVSTSNRNFEGRQGPGARTMLASPLTAAAAAITGVVTDPRTLLN

Sequences:

>Translated_474_residues
MNKPRTLFEKVWEAHLVRPETAETPAVLYIDLHLIHEVTSPQAFTELRQRGLRVRRPDKTLATMDHSTPTTPRNHLGIIP
VVDPMAISQLEQLRKNCAEFGIPLFELGDENQGIVHVIGPEQGLTQPGMTIVCGDSHTSTHGAFGALAFGIGTSEVGHVL
ATQCLLQRKPKTCAVRIDGRLGPGVTAKDIILALIAKYGVGGGTGYVFEYMGEAIRALSMEERMTICNMSIEGGARAGMV
APDDTTFEYIAGRPFAPKGADFEAAVARWRTLPSDEGATFDHELTLSASELKPMITYGTNPGMGIPIDAPVPRPEDMPDA
RSRAALDKALAYMGLEPGKPLLGHPVDVVFIGSCTNSRLSDLRQAAQFFRGRKVAPGVRVMVVPGSQQVKRAAEAEGLDR
IFKEAGAEWREAGCSACLGMNDDKVPPGKYAVSTSNRNFEGRQGPGARTMLASPLTAAAAAITGVVTDPRTLLN
>Mature_474_residues
MNKPRTLFEKVWEAHLVRPETAETPAVLYIDLHLIHEVTSPQAFTELRQRGLRVRRPDKTLATMDHSTPTTPRNHLGIIP
VVDPMAISQLEQLRKNCAEFGIPLFELGDENQGIVHVIGPEQGLTQPGMTIVCGDSHTSTHGAFGALAFGIGTSEVGHVL
ATQCLLQRKPKTCAVRIDGRLGPGVTAKDIILALIAKYGVGGGTGYVFEYMGEAIRALSMEERMTICNMSIEGGARAGMV
APDDTTFEYIAGRPFAPKGADFEAAVARWRTLPSDEGATFDHELTLSASELKPMITYGTNPGMGIPIDAPVPRPEDMPDA
RSRAALDKALAYMGLEPGKPLLGHPVDVVFIGSCTNSRLSDLRQAAQFFRGRKVAPGVRVMVVPGSQQVKRAAEAEGLDR
IFKEAGAEWREAGCSACLGMNDDKVPPGKYAVSTSNRNFEGRQGPGARTMLASPLTAAAAAITGVVTDPRTLLN

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=492, Percent_Identity=27.0325203252033, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI41352693, Length=390, Percent_Identity=25.1282051282051, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI1786259, Length=468, Percent_Identity=60.6837606837607, Blast_Score=563, Evalue=1e-161,
Organism=Escherichia coli, GI1787531, Length=375, Percent_Identity=27.2, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI2367097, Length=337, Percent_Identity=26.7062314540059, Blast_Score=69, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI25149337, Length=488, Percent_Identity=26.844262295082, Blast_Score=128, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI32564738, Length=378, Percent_Identity=28.8359788359788, Blast_Score=123, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI25149342, Length=306, Percent_Identity=29.0849673202614, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17568399, Length=457, Percent_Identity=25.164113785558, Blast_Score=90, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6321429, Length=454, Percent_Identity=56.8281938325991, Blast_Score=528, Evalue=1e-151,
Organism=Saccharomyces cerevisiae, GI6322261, Length=491, Percent_Identity=26.8839103869654, Blast_Score=142, Evalue=9e-35,
Organism=Saccharomyces cerevisiae, GI6323335, Length=481, Percent_Identity=27.8586278586279, Blast_Score=139, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6320440, Length=495, Percent_Identity=25.4545454545455, Blast_Score=134, Evalue=2e-32,
Organism=Drosophila melanogaster, GI281365315, Length=488, Percent_Identity=26.2295081967213, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI17864292, Length=488, Percent_Identity=26.2295081967213, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI161076999, Length=382, Percent_Identity=27.7486910994764, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI28571643, Length=483, Percent_Identity=25.0517598343685, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI17137564, Length=381, Percent_Identity=25.9842519685039, Blast_Score=85, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24645686, Length=371, Percent_Identity=26.1455525606469, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 50887; Mature: 50887

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKPRTLFEKVWEAHLVRPETAETPAVLYIDLHLIHEVTSPQAFTELRQRGLRVRRPDKT
CCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEHHHHHCCCHHHHHHHHCCCEEECCCCH
LATMDHSTPTTPRNHLGIIPVVDPMAISQLEQLRKNCAEFGIPLFELGDENQGIVHVIGP
HEECCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEECC
EQGLTQPGMTIVCGDSHTSTHGAFGALAFGIGTSEVGHVLATQCLLQRKPKTCAVRIDGR
CCCCCCCCCEEEECCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCC
LGPGVTAKDIILALIAKYGVGGGTGYVFEYMGEAIRALSMEERMTICNMSIEGGARAGMV
CCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCC
APDDTTFEYIAGRPFAPKGADFEAAVARWRTLPSDEGATFDHELTLSASELKPMITYGTN
CCCCCCHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEHHHCCEEEEECCC
PGMGIPIDAPVPRPEDMPDARSRAALDKALAYMGLEPGKPLLGHPVDVVFIGSCTNSRLS
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCHHHH
DLRQAAQFFRGRKVAPGVRVMVVPGSQQVKRAAEAEGLDRIFKEAGAEWREAGCSACLGM
HHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCHHHHHCHHEECCC
NDDKVPPGKYAVSTSNRNFEGRQGPGARTMLASPLTAAAAAITGVVTDPRTLLN
CCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHCHHHHHHHHHHHCCCCCHHHCC
>Mature Secondary Structure
MNKPRTLFEKVWEAHLVRPETAETPAVLYIDLHLIHEVTSPQAFTELRQRGLRVRRPDKT
CCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEHHHHHCCCHHHHHHHHCCCEEECCCCH
LATMDHSTPTTPRNHLGIIPVVDPMAISQLEQLRKNCAEFGIPLFELGDENQGIVHVIGP
HEECCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEECC
EQGLTQPGMTIVCGDSHTSTHGAFGALAFGIGTSEVGHVLATQCLLQRKPKTCAVRIDGR
CCCCCCCCCEEEECCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCC
LGPGVTAKDIILALIAKYGVGGGTGYVFEYMGEAIRALSMEERMTICNMSIEGGARAGMV
CCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCC
APDDTTFEYIAGRPFAPKGADFEAAVARWRTLPSDEGATFDHELTLSASELKPMITYGTN
CCCCCCHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEHHHCCEEEEECCC
PGMGIPIDAPVPRPEDMPDARSRAALDKALAYMGLEPGKPLLGHPVDVVFIGSCTNSRLS
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCHHHH
DLRQAAQFFRGRKVAPGVRVMVVPGSQQVKRAAEAEGLDRIFKEAGAEWREAGCSACLGM
HHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCHHHHHCHHEECCC
NDDKVPPGKYAVSTSNRNFEGRQGPGARTMLASPLTAAAAAITGVVTDPRTLLN
CCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHCHHHHHHHHHHHCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA