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The map label for this gene is kanE [H]

Identifier: 222523465

GI number: 222523465

Start: 195862

End: 196884

Strand: Direct

Name: kanE [H]

Synonym: Chy400_0170

Alternate gene names: 222523465

Gene position: 195862-196884 (Clockwise)

Preceding gene: 222523464

Following gene: 222523466

Centisome position: 3.72

GC content: 59.43

Gene sequence:

>1023_bases
ATGAAACCCTTACCCGCGCAAGCCATCTGGTTTACCGCCCCCCACACGGTGAGCATCCGTGAGGAGATTGCTCCACCACC
GGCTTACGGTGACGTGCGGATCGCAGCTATTGCATCGTTGATCAGTCACGGCACCGAACGCCTGGTCTATCGCGGTGAGG
TCGATCCAACCCTGGCCCTCGATCTCCCAACGCTGCAAGGAAGTTTTGCCTTTCCCATCAAATACGGTTACGCCATCGCC
GGCAGAGTCATCGACATTGGCCCAGGGGTTGAGCAGTTTCAGATTGGTGATCCGGTCGCGGCCCTCCATCCGCACCAGAC
TATCTTCACCCTACCGGCGCATCTGGTCAAACGGCTACCGGACACCCTTGATCCGGCATTGGGTGGGTTCTACGCCAATG
TCGAAACAGCGCTGACGATTTGCCACGATGCAGCACCAAGGCTGGGAGAAACTGTCGTTGTGCTGGGACAGGGTGTGGTT
GGCTTGCTGGTCACACAGCTCTTGCAACGAACCGGTGCGCAGGTGATTGCGGTCGAACCCGATGCGCATCGTCGGGCGCT
GGCCACCCAATTCGGTGCCACGACCCTTGCCCAGGCTGATCCGGCAACCATTGCCGAACTTACCGATGGACGGGGGGCCG
ATATAGTGGTTGAGGTGAGCGGGTCACCGGCTGCGTTACAACAGGCGATTGAACTAACAGCGACAGAGGGGCTGGTGGTG
GTTGCATCGTGGTACGGGCAAAAACCGGCACCACTGATCCTGGGAGGGCATTTTCACCGTGGTCGGGTACGCCTGCGCTC
ATCGCAGGTTGGTCGGCTGGCTCCAGAAACGCTGCCACGCTGGGATTACGCACGGCGGAGCGCCACCGTGATGAACCTCT
TACCGCGCCTATGCCTGAACGAGTTGATCAGCCACCGCATTCCTTTCGTGCAAGCCGCAGCAGCGTATGATCTGCTTGAT
CAGGCACCGCCAGGGCTGGTACAGGTGATGTTGACGTATGCTGATTACGCACCACAAACGTGA

Upstream 100 bases:

>100_bases
CGACTGACGAACGTCACCTGGACGACGTATGGCGCTGATGTGGTGATCGAAGGCGATCTGGCACACCATCAGTAGCAATC
AGCGAAGCAGTCAAGACGGT

Downstream 100 bases:

>100_bases
ACGATGGGAGTGATAGGCCCGCCGGAATCGCTCTCGTCCCTGGGCCGTGCTGGTGTGGTGACCAGCCGGGGTGCAGCGAA
CGAAAACTATCGTCTGTAAG

Product: alcohol dehydrogenase zinc-binding domain-containing protein

Products: aldehyde; ketone; NADH; H+

Alternate protein names: DOIA dehydrogenase [H]

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MKPLPAQAIWFTAPHTVSIREEIAPPPAYGDVRIAAIASLISHGTERLVYRGEVDPTLALDLPTLQGSFAFPIKYGYAIA
GRVIDIGPGVEQFQIGDPVAALHPHQTIFTLPAHLVKRLPDTLDPALGGFYANVETALTICHDAAPRLGETVVVLGQGVV
GLLVTQLLQRTGAQVIAVEPDAHRRALATQFGATTLAQADPATIAELTDGRGADIVVEVSGSPAALQQAIELTATEGLVV
VASWYGQKPAPLILGGHFHRGRVRLRSSQVGRLAPETLPRWDYARRSATVMNLLPRLCLNELISHRIPFVQAAAAYDLLD
QAPPGLVQVMLTYADYAPQT

Sequences:

>Translated_340_residues
MKPLPAQAIWFTAPHTVSIREEIAPPPAYGDVRIAAIASLISHGTERLVYRGEVDPTLALDLPTLQGSFAFPIKYGYAIA
GRVIDIGPGVEQFQIGDPVAALHPHQTIFTLPAHLVKRLPDTLDPALGGFYANVETALTICHDAAPRLGETVVVLGQGVV
GLLVTQLLQRTGAQVIAVEPDAHRRALATQFGATTLAQADPATIAELTDGRGADIVVEVSGSPAALQQAIELTATEGLVV
VASWYGQKPAPLILGGHFHRGRVRLRSSQVGRLAPETLPRWDYARRSATVMNLLPRLCLNELISHRIPFVQAAAAYDLLD
QAPPGLVQVMLTYADYAPQT
>Mature_340_residues
MKPLPAQAIWFTAPHTVSIREEIAPPPAYGDVRIAAIASLISHGTERLVYRGEVDPTLALDLPTLQGSFAFPIKYGYAIA
GRVIDIGPGVEQFQIGDPVAALHPHQTIFTLPAHLVKRLPDTLDPALGGFYANVETALTICHDAAPRLGETVVVLGQGVV
GLLVTQLLQRTGAQVIAVEPDAHRRALATQFGATTLAQADPATIAELTDGRGADIVVEVSGSPAALQQAIELTATEGLVV
VASWYGQKPAPLILGGHFHRGRVRLRSSQVGRLAPETLPRWDYARRSATVMNLLPRLCLNELISHRIPFVQAAAAYDLLD
QAPPGLVQVMLTYADYAPQT

Specific function: Catalyzes the oxidation of 2-deoxy-scyllo-inosamine (DOIA) with NAD(+) or NADP(+), forming 3-amino-2,3-dideoxy-scyllo- inosose (amino-DOI) [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. DOIA dehydrogenase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787572, Length=272, Percent_Identity=27.2058823529412, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.1.1.1

Molecular weight: Translated: 36372; Mature: 36372

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPLPAQAIWFTAPHTVSIREEIAPPPAYGDVRIAAIASLISHGTERLVYRGEVDPTLAL
CCCCCCCEEEEECCCEEEHHHHCCCCCCCCCHHHHHHHHHHHCCCHHEEEECCCCCEEEE
DLPTLQGSFAFPIKYGYAIAGRVIDIGPGVEQFQIGDPVAALHPHQTIFTLPAHLVKRLP
ECCCCCCCEECEEEECEEEECEEEECCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHCC
DTLDPALGGFYANVETALTICHDAAPRLGETVVVLGQGVVGLLVTQLLQRTGAQVIAVEP
HHCCHHHCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHCCCEEEEECC
DAHRRALATQFGATTLAQADPATIAELTDGRGADIVVEVSGSPAALQQAIELTATEGLVV
CHHHHHHHHHHCCHHHCCCCCCHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHCCCCEEE
VASWYGQKPAPLILGGHFHRGRVRLRSSQVGRLAPETLPRWDYARRSATVMNLLPRLCLN
EEECCCCCCCCEEEECCCCCCEEEECCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHH
ELISHRIPFVQAAAAYDLLDQAPPGLVQVMLTYADYAPQT
HHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKPLPAQAIWFTAPHTVSIREEIAPPPAYGDVRIAAIASLISHGTERLVYRGEVDPTLAL
CCCCCCCEEEEECCCEEEHHHHCCCCCCCCCHHHHHHHHHHHCCCHHEEEECCCCCEEEE
DLPTLQGSFAFPIKYGYAIAGRVIDIGPGVEQFQIGDPVAALHPHQTIFTLPAHLVKRLP
ECCCCCCCEECEEEECEEEECEEEECCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHCC
DTLDPALGGFYANVETALTICHDAAPRLGETVVVLGQGVVGLLVTQLLQRTGAQVIAVEP
HHCCHHHCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHCCCEEEEECC
DAHRRALATQFGATTLAQADPATIAELTDGRGADIVVEVSGSPAALQQAIELTATEGLVV
CHHHHHHHHHHCCHHHCCCCCCHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHCCCCEEE
VASWYGQKPAPLILGGHFHRGRVRLRSSQVGRLAPETLPRWDYARRSATVMNLLPRLCLN
EEECCCCCCCCEEEECCCCCCEEEECCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHH
ELISHRIPFVQAAAAYDLLDQAPPGLVQVMLTYADYAPQT
HHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: alcohol; NAD+

Specific reaction: an alcohol + NAD+ = an aldehyde or ketone + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA