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The map label for this gene is ygcM [C]

Identifier: 222523466

GI number: 222523466

Start: 197076

End: 197456

Strand: Direct

Name: ygcM [C]

Synonym: Chy400_0171

Alternate gene names: 222523466

Gene position: 197076-197456 (Clockwise)

Preceding gene: 222523465

Following gene: 222523467

Centisome position: 3.74

GC content: 58.27

Gene sequence:

>381_bases
ATGTATGAAGTCGGCGTCATTGCCCAGTTTGAAGCGGCTCATCGTTTGGAAGGGAACTTCGGCCCAGCCACCCGGATGCA
CGGGCATACCTACCGGCTGGAGGCGATTGTGAGCGGGGAGCGTCTGCAAGCGGACGGCACCCTCTTCGACATTACACGCC
TGCAAGAGGCGGTCAACGCCATCGTTGCCGACCTGCACTACCGTGATTTGGGTACTGTCGCCGGTCTGGAGAACGTGAAT
ACCACTGCTGAAAACGTGGCGCGCTATTGCTGGGATCGGATTGCGGCTGCCATTCGTGGCGTTGGTTTAATGACGCTCAC
CGTGCGCATCTGGGAAAATCCGCAAGCCTTCGCCAGTTATCAGAGCAGGCTGGAAGGATGA

Upstream 100 bases:

>100_bases
GTCTGTAAGGATGCGATACATCCCAATAACACGGGCCAGTAGAAGATGGCCCGGATTTCTGAGAAGCGAAGACCTCGCGC
TCTGTGCAAAGGAGACACGT

Downstream 100 bases:

>100_bases
AGGTATGGCCCTGCGCATTGCGTTTCTCACCGTCGGCGATCCGCAACGGCGTACCGGCGGCTACCTCTACCATCGCGAGG
TCTTTCGGTGCTGGCAGGCT

Product: hypothetical protein

Products: 6-pyruvoyl-5,6,7,8-tetrahydropterin; triphosphate

Alternate protein names: 6-Pyruvoyl-Tetrahydropterin Synthase; Queuosine Biosynthesis Protein QueD; 6-Pyruvoyltetrahydropterin Synthase; 6-Pyruvoyl Tetrahydrobiopterin Synthase; Preq(0) Biosynthesis Protein Qued

Number of amino acids: Translated: 126; Mature: 126

Protein sequence:

>126_residues
MYEVGVIAQFEAAHRLEGNFGPATRMHGHTYRLEAIVSGERLQADGTLFDITRLQEAVNAIVADLHYRDLGTVAGLENVN
TTAENVARYCWDRIAAAIRGVGLMTLTVRIWENPQAFASYQSRLEG

Sequences:

>Translated_126_residues
MYEVGVIAQFEAAHRLEGNFGPATRMHGHTYRLEAIVSGERLQADGTLFDITRLQEAVNAIVADLHYRDLGTVAGLENVN
TTAENVARYCWDRIAAAIRGVGLMTLTVRIWENPQAFASYQSRLEG
>Mature_126_residues
MYEVGVIAQFEAAHRLEGNFGPATRMHGHTYRLEAIVSGERLQADGTLFDITRLQEAVNAIVADLHYRDLGTVAGLENVN
TTAENVARYCWDRIAAAIRGVGLMTLTVRIWENPQAFASYQSRLEG

Specific function: Tetrahydrobiopterin biosynthesis; second step. [C]

COG id: COG0720

COG function: function code H; 6-pyruvoyl-tetrahydropterin synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 4.2.3.12

Molecular weight: Translated: 13980; Mature: 13980

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYEVGVIAQFEAAHRLEGNFGPATRMHGHTYRLEAIVSGERLQADGTLFDITRLQEAVNA
CCCCCEEEHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCEEECCCCCHHHHHHHHHHHH
IVADLHYRDLGTVAGLENVNTTAENVARYCWDRIAAAIRGVGLMTLTVRIWENPQAFASY
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCHHHHHH
QSRLEG
HHHCCC
>Mature Secondary Structure
MYEVGVIAQFEAAHRLEGNFGPATRMHGHTYRLEAIVSGERLQADGTLFDITRLQEAVNA
CCCCCEEEHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCEEECCCCCHHHHHHHHHHHH
IVADLHYRDLGTVAGLENVNTTAENVARYCWDRIAAAIRGVGLMTLTVRIWENPQAFASY
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCHHHHHH
QSRLEG
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Mg2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 17.7 {6-(L-erythro-1,2-dihydroxypropyl} 5 {6-(L-erythro-1,2-dihydroxypropyl} 0.0091 {6-(L-erythro-1,2-dihydroxypropyl} 0.008 {6-(L-erythro-1,2-dihydroxypropyl} [C]

Substrates: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin

Specific reaction: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin = 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate

General reaction: Intramolecular redox reaction; P-O bond cleavage; Triphosphate elimination [C]

Inhibitor: (NH4)2SO4 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA