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The map label for this gene is pcm2 [H]

Identifier: 220932282

GI number: 220932282

Start: 1537470

End: 1538141

Strand: Direct

Name: pcm2 [H]

Synonym: Hore_14460

Alternate gene names: 220932282

Gene position: 1537470-1538141 (Clockwise)

Preceding gene: 220932274

Following gene: 220932283

Centisome position: 59.63

GC content: 44.05

Gene sequence:

>672_bases
ATGTTATGGTCCCAAAACCAGGCTTATACTCCCGAAGAAATGATAAAAAAACACCTTAAACCCAGGGGGATAAAAAACAA
ACTTGTTTTAAAGGCGATGAGGCAGGTTCCCCGCCATGTGTTTGTTCCTGAAAAGTTTAAGGCCATAGCCTATTATGACC
GGGCCCTGCCTATCGATAAAGGTCAGACCATAAGCCAGCCTTATATTGTAGCTCTTATGATCCAGACACTAAATCCCAAA
AAAAGCCACCGTATTCTGGAAATAGGGACCGGTTCTGGCTACGCAGCTGCCGTCCTATCTCATATTGCCAGACAGGTTTA
TACAGTGGAAAGAATAGAACAGCTGGCCAACCGGGCCCGGGAATTATTTAATAAATTAGATTATAGTAATATTGAAGTTA
AAGTTGATGATGGGAGCCTGGGGTGGTCTGAAAAAGCACCATTTGATGGAATAATAGTCTCCGCAGGGGCTCCGGCTATA
CCTGAAACCCTTACAGAACAACTTAAAGAAGGGGGTCGGCTGGTAATACCTGTCGGAAAAAGAGGAGGACTCCAGTATTT
AACGGTAGTCACCAGGGACAAAAATGGAGAAATAAATCAACAAGAACTGGAGCCGGTTCGTTTCGTACCCCTCATTGGCC
AGGAAGGCTTTAAAGAAAATAACCAGGAATAG

Upstream 100 bases:

>100_bases
ATCATTAAAAACTAACATTACAGTATAAGCAAACAGGAAAATTATCAAATAATAAGGAAATATATTTTAACATCTATTTA
TAATAACCGGGGTGATAAAC

Downstream 100 bases:

>100_bases
TTATTTAATCAGAACTAATAGTAAAAATAACCACAAATAATAAGTGAATAATATGTAGTAAAACAGTGCATTATAACAAT
TGAGTTAATTGAGTCATAAA

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase 2; Protein L-isoaspartyl methyltransferase 2; Protein-beta-aspartate methyltransferase 2; PIMT 2 [H]

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MLWSQNQAYTPEEMIKKHLKPRGIKNKLVLKAMRQVPRHVFVPEKFKAIAYYDRALPIDKGQTISQPYIVALMIQTLNPK
KSHRILEIGTGSGYAAAVLSHIARQVYTVERIEQLANRARELFNKLDYSNIEVKVDDGSLGWSEKAPFDGIIVSAGAPAI
PETLTEQLKEGGRLVIPVGKRGGLQYLTVVTRDKNGEINQQELEPVRFVPLIGQEGFKENNQE

Sequences:

>Translated_223_residues
MLWSQNQAYTPEEMIKKHLKPRGIKNKLVLKAMRQVPRHVFVPEKFKAIAYYDRALPIDKGQTISQPYIVALMIQTLNPK
KSHRILEIGTGSGYAAAVLSHIARQVYTVERIEQLANRARELFNKLDYSNIEVKVDDGSLGWSEKAPFDGIIVSAGAPAI
PETLTEQLKEGGRLVIPVGKRGGLQYLTVVTRDKNGEINQQELEPVRFVPLIGQEGFKENNQE
>Mature_223_residues
MLWSQNQAYTPEEMIKKHLKPRGIKNKLVLKAMRQVPRHVFVPEKFKAIAYYDRALPIDKGQTISQPYIVALMIQTLNPK
KSHRILEIGTGSGYAAAVLSHIARQVYTVERIEQLANRARELFNKLDYSNIEVKVDDGSLGWSEKAPFDGIIVSAGAPAI
PETLTEQLKEGGRLVIPVGKRGGLQYLTVVTRDKNGEINQQELEPVRFVPLIGQEGFKENNQE

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI226530908, Length=225, Percent_Identity=30.6666666666667, Blast_Score=96, Evalue=3e-20,
Organism=Escherichia coli, GI1789100, Length=198, Percent_Identity=47.979797979798, Blast_Score=173, Evalue=8e-45,
Organism=Caenorhabditis elegans, GI71983477, Length=225, Percent_Identity=29.7777777777778, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI193207222, Length=225, Percent_Identity=29.3333333333333, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI17981723, Length=227, Percent_Identity=32.5991189427313, Blast_Score=106, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 25103; Mature: 25103

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: PS01279 PCMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLWSQNQAYTPEEMIKKHLKPRGIKNKLVLKAMRQVPRHVFVPEKFKAIAYYDRALPIDK
CCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCC
GQTISQPYIVALMIQTLNPKKSHRILEIGTGSGYAAAVLSHIARQVYTVERIEQLANRAR
CCCCCCCCEEEHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELFNKLDYSNIEVKVDDGSLGWSEKAPFDGIIVSAGAPAIPETLTEQLKEGGRLVIPVGK
HHHHHCCCCCEEEEECCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHCCCEEEEEECC
RGGLQYLTVVTRDKNGEINQQELEPVRFVPLIGQEGFKENNQE
CCCEEEEEEEEECCCCCCCHHHCCCEEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MLWSQNQAYTPEEMIKKHLKPRGIKNKLVLKAMRQVPRHVFVPEKFKAIAYYDRALPIDK
CCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCC
GQTISQPYIVALMIQTLNPKKSHRILEIGTGSGYAAAVLSHIARQVYTVERIEQLANRAR
CCCCCCCCEEEHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELFNKLDYSNIEVKVDDGSLGWSEKAPFDGIIVSAGAPAIPETLTEQLKEGGRLVIPVGK
HHHHHCCCCCEEEEECCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHCCCEEEEEECC
RGGLQYLTVVTRDKNGEINQQELEPVRFVPLIGQEGFKENNQE
CCCEEEEEEEEECCCCCCCHHHCCCEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA