Click here to switch to the map view.

The map label for this gene is fadD26 [H]

Identifier: 221230751

GI number: 221230751

Start: 2821604

End: 2823355

Strand: Reverse

Name: fadD26 [H]

Synonym: MLBr_02358

Alternate gene names: 221230751

Gene position: 2823355-2821604 (Counterclockwise)

Preceding gene: 221230752

Following gene: 221230750

Centisome position: 86.39

GC content: 55.76

Gene sequence:

>1752_bases
ATGGTCGTAACCAACTTTTCCGTCCCCGCTTTGCTGAAAGAACGGGCGGATCAGCAGGCTGACACTACCGCCTACACATA
CATCGACTACGGGTCGGACCCGAAGGGTTTTGTTGAGAGCTTGACGTGGTCGCAAGTTTATATGCGTTCATGCGTCCTTG
CTGACGAACTCAAATTGTGTGGTGTGCCCGGTGATCGAGTTGCAATTTTGGCGCCACAGGGACTGGAATATATTATCGCG
TTCTTAGGGGCTCTTCAGGCCGGATTTATCGCGGTACCTCTTTCGCCACCGCAGTATGCGATTCACGACGAACGCGTTTC
GGCGGTGTTACGGGATTCTCAACCGATCGCTATTCTTACAACTTCATTTGTGGTTAGTGACGTGGCGAAATACGCATGTG
AACAAGATGGGTGGAACGCTCCGTCTGTCATTGAGATCGATCTGCTTGACTTGAACGCGTCACTCCCGCTTCCAAAAGTC
CCCCAGCCTTTTACTAGCCCGGCTTACCTCCAATACACATCCGGGTCGACACGTACGCCAGCCGGTGTTATGGTGTCGCA
TAAGAACGTTATCGCTAACGTGCAGCAAAGCATGAACGGCTATTTCGGCGATGCCGCAAAAACTCTGAACACGACCGTGG
TGTCATGGCTTCCCTTGTTTCACGACATGGGCTTGATTCTTGGGGTCTGTGCTCCAATGGTCGCGGGGCGAAGCTCGGTG
TTGTTGAGCCCTATGTCATTTTTGCGGCGCCCCGCCTGTTGGATGCAACTTCTTGCCACTACCCAGGCGTGTTTTTCTGC
GGCACCGAATTTTGCCTTTGAGCTGGCCGTACGCAGAACGTCCGACGACGACATGAACGGACTCGACCTCGGGGATGTGG
CCGGAATCATCAGCGGCAGCGAACGAGTCCATATCGCAACGGTGAAACGTTTCACCGAGCGCTTCGCTCGGTTCAATCTC
AGCCCCTCGGTGGTACGACCCTCGTACGGGCTTGCGGAAGCGACCGTGTACGTGGCGGCTCCGGAACCTGGCACCGCACC
TAAGGCGGTTCGTTTTGACTACGAATACCTGACGGCCAGTAAGGCTAAGGCCCGCCGAGCCGAAGGGAGTGTCGCGACCG
AACTAATTAGTTATGGCTCCCCTGATGCATCGGCTGTGCGAATCGTCGATCCGGAGTCCATGATCGAGAACCCTGCTGGA
ATGGTTGGGGAGATCTGGGTACAGGGCGACCACGTTGCCATGGGGTATTGGCGGAAGCCCGAGCAGACTGCACGTACGTT
CAATGCAAAGATTGTCAACCCCGCGCCTGGCACTTCGGAAGGTCCGTGGCTGCGGACCGGAGATCTGGGTGTTATGTCAG
ACGGCGAACTGTTCATCATGGGCCGCATCAAAGACTTGCTCATTGTCGATGGTCGCAACCACTACCCGGACGACATCGAA
GCGACAATCCAGGAGATCACCGGCGGTCGGGTCGCGGCGATCACTGTGCCGGACGACCTCACCGAGCAGTTGGTGGCGAT
CATTGAATACAAGGAACATGGAGCGTCCGCGGAAGACCACATGCACAAAGTCCATTCGGTGAAGCGTGAAGTCACATCCG
CTATATCGAAGTTACATAGTTTGCATGTGGCCGACCTCGTTCTGGTGTCACCGGGCTCAATCCCGATCACCACAAGCGGC
AAGGTCCGACGTTCGGCCTGTGTCGAACGTTATCGCAGTGACGGATTCAAACGACTGGACGTGACGGTATGA

Upstream 100 bases:

>100_bases
GAGCTGTGCGATCCATCGGGGGGGTAGCACAGTTCGGGTACATCATGAACGCGGTCAGGCCAATGAGCTGTCAAACCGAA
CGGAAGGTGGAAGGTGTGGT

Downstream 100 bases:

>100_bases
CGGCGAGCATTGGTAGCGAAGCTGACCTTTGCCACTGGCTAGTCAACTACTTGGTGACTAATATCGGCTGCACACCGGAT
GAAGTCGATCTTAATCTATC

Product: acyl-CoA synthetase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 583; Mature: 583

Protein sequence:

>583_residues
MVVTNFSVPALLKERADQQADTTAYTYIDYGSDPKGFVESLTWSQVYMRSCVLADELKLCGVPGDRVAILAPQGLEYIIA
FLGALQAGFIAVPLSPPQYAIHDERVSAVLRDSQPIAILTTSFVVSDVAKYACEQDGWNAPSVIEIDLLDLNASLPLPKV
PQPFTSPAYLQYTSGSTRTPAGVMVSHKNVIANVQQSMNGYFGDAAKTLNTTVVSWLPLFHDMGLILGVCAPMVAGRSSV
LLSPMSFLRRPACWMQLLATTQACFSAAPNFAFELAVRRTSDDDMNGLDLGDVAGIISGSERVHIATVKRFTERFARFNL
SPSVVRPSYGLAEATVYVAAPEPGTAPKAVRFDYEYLTASKAKARRAEGSVATELISYGSPDASAVRIVDPESMIENPAG
MVGEIWVQGDHVAMGYWRKPEQTARTFNAKIVNPAPGTSEGPWLRTGDLGVMSDGELFIMGRIKDLLIVDGRNHYPDDIE
ATIQEITGGRVAAITVPDDLTEQLVAIIEYKEHGASAEDHMHKVHSVKREVTSAISKLHSLHVADLVLVSPGSIPITTSG
KVRRSACVERYRSDGFKRLDVTV

Sequences:

>Translated_583_residues
MVVTNFSVPALLKERADQQADTTAYTYIDYGSDPKGFVESLTWSQVYMRSCVLADELKLCGVPGDRVAILAPQGLEYIIA
FLGALQAGFIAVPLSPPQYAIHDERVSAVLRDSQPIAILTTSFVVSDVAKYACEQDGWNAPSVIEIDLLDLNASLPLPKV
PQPFTSPAYLQYTSGSTRTPAGVMVSHKNVIANVQQSMNGYFGDAAKTLNTTVVSWLPLFHDMGLILGVCAPMVAGRSSV
LLSPMSFLRRPACWMQLLATTQACFSAAPNFAFELAVRRTSDDDMNGLDLGDVAGIISGSERVHIATVKRFTERFARFNL
SPSVVRPSYGLAEATVYVAAPEPGTAPKAVRFDYEYLTASKAKARRAEGSVATELISYGSPDASAVRIVDPESMIENPAG
MVGEIWVQGDHVAMGYWRKPEQTARTFNAKIVNPAPGTSEGPWLRTGDLGVMSDGELFIMGRIKDLLIVDGRNHYPDDIE
ATIQEITGGRVAAITVPDDLTEQLVAIIEYKEHGASAEDHMHKVHSVKREVTSAISKLHSLHVADLVLVSPGSIPITTSG
KVRRSACVERYRSDGFKRLDVTV
>Mature_583_residues
MVVTNFSVPALLKERADQQADTTAYTYIDYGSDPKGFVESLTWSQVYMRSCVLADELKLCGVPGDRVAILAPQGLEYIIA
FLGALQAGFIAVPLSPPQYAIHDERVSAVLRDSQPIAILTTSFVVSDVAKYACEQDGWNAPSVIEIDLLDLNASLPLPKV
PQPFTSPAYLQYTSGSTRTPAGVMVSHKNVIANVQQSMNGYFGDAAKTLNTTVVSWLPLFHDMGLILGVCAPMVAGRSSV
LLSPMSFLRRPACWMQLLATTQACFSAAPNFAFELAVRRTSDDDMNGLDLGDVAGIISGSERVHIATVKRFTERFARFNL
SPSVVRPSYGLAEATVYVAAPEPGTAPKAVRFDYEYLTASKAKARRAEGSVATELISYGSPDASAVRIVDPESMIENPAG
MVGEIWVQGDHVAMGYWRKPEQTARTFNAKIVNPAPGTSEGPWLRTGDLGVMSDGELFIMGRIKDLLIVDGRNHYPDDIE
ATIQEITGGRVAAITVPDDLTEQLVAIIEYKEHGASAEDHMHKVHSVKREVTSAISKLHSLHVADLVLVSPGSIPITTSG
KVRRSACVERYRSDGFKRLDVTV

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI44888818, Length=520, Percent_Identity=25.1923076923077, Blast_Score=106, Evalue=6e-23,
Organism=Homo sapiens, GI55749758, Length=610, Percent_Identity=26.0655737704918, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI225735629, Length=562, Percent_Identity=24.5551601423488, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI45827692, Length=562, Percent_Identity=24.5551601423488, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI225735625, Length=582, Percent_Identity=25.085910652921, Blast_Score=89, Evalue=9e-18,
Organism=Homo sapiens, GI187761345, Length=585, Percent_Identity=23.9316239316239, Blast_Score=88, Evalue=3e-17,
Organism=Homo sapiens, GI187761343, Length=585, Percent_Identity=23.9316239316239, Blast_Score=88, Evalue=3e-17,
Organism=Escherichia coli, GI1788107, Length=582, Percent_Identity=25.6013745704467, Blast_Score=111, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI193202679, Length=559, Percent_Identity=24.1502683363148, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17531443, Length=450, Percent_Identity=24.2222222222222, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17559526, Length=499, Percent_Identity=24.248496993988, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24581924, Length=555, Percent_Identity=23.6036036036036, Blast_Score=95, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24654656, Length=476, Percent_Identity=23.5294117647059, Blast_Score=84, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 63213; Mature: 63213

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVTNFSVPALLKERADQQADTTAYTYIDYGSDPKGFVESLTWSQVYMRSCVLADELKLC
CEEECCCCHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
GVPGDRVAILAPQGLEYIIAFLGALQAGFIAVPLSPPQYAIHDERVSAVLRDSQPIAILT
CCCCCCEEEECCCCHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEE
TSFVVSDVAKYACEQDGWNAPSVIEIDLLDLNASLPLPKVPQPFTSPAYLQYTSGSTRTP
HHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCEEEECCCCCCCC
AGVMVSHKNVIANVQQSMNGYFGDAAKTLNTTVVSWLPLFHDMGLILGVCAPMVAGRSSV
CCEEEECHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
LLSPMSFLRRPACWMQLLATTQACFSAAPNFAFELAVRRTSDDDMNGLDLGDVAGIISGS
EECHHHHHHCHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCHHHHHHHCCCC
ERVHIATVKRFTERFARFNLSPSVVRPSYGLAEATVYVAAPEPGTAPKAVRFDYEYLTAS
CEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEECHHHHHHH
KAKARRAEGSVATELISYGSPDASAVRIVDPESMIENPAGMVGEIWVQGDHVAMGYWRKP
HHHHHHCCCHHHHHHHHCCCCCCCEEEEECCHHHHCCCCCCEEEEEEECCEEEECCCCCC
EQTARTFNAKIVNPAPGTSEGPWLRTGDLGVMSDGELFIMGRIKDLLIVDGRNHYPDDIE
HHHHHHCCCEEECCCCCCCCCCEEEECCCCEECCCCEEEEECCCEEEEECCCCCCCCHHH
ATIQEITGGRVAAITVPDDLTEQLVAIIEYKEHGASAEDHMHKVHSVKREVTSAISKLHS
HHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LHVADLVLVSPGSIPITTSGKVRRSACVERYRSDGFKRLDVTV
HHHEEEEEECCCCEEEECCCCHHHHHHHHHHHCCCCEEEEEEC
>Mature Secondary Structure
MVVTNFSVPALLKERADQQADTTAYTYIDYGSDPKGFVESLTWSQVYMRSCVLADELKLC
CEEECCCCHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
GVPGDRVAILAPQGLEYIIAFLGALQAGFIAVPLSPPQYAIHDERVSAVLRDSQPIAILT
CCCCCCEEEECCCCHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEE
TSFVVSDVAKYACEQDGWNAPSVIEIDLLDLNASLPLPKVPQPFTSPAYLQYTSGSTRTP
HHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCEEEECCCCCCCC
AGVMVSHKNVIANVQQSMNGYFGDAAKTLNTTVVSWLPLFHDMGLILGVCAPMVAGRSSV
CCEEEECHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
LLSPMSFLRRPACWMQLLATTQACFSAAPNFAFELAVRRTSDDDMNGLDLGDVAGIISGS
EECHHHHHHCHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCHHHHHHHCCCC
ERVHIATVKRFTERFARFNLSPSVVRPSYGLAEATVYVAAPEPGTAPKAVRFDYEYLTAS
CEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEECHHHHHHH
KAKARRAEGSVATELISYGSPDASAVRIVDPESMIENPAGMVGEIWVQGDHVAMGYWRKP
HHHHHHCCCHHHHHHHHCCCCCCCEEEEECCHHHHCCCCCCEEEEEEECCEEEECCCCCC
EQTARTFNAKIVNPAPGTSEGPWLRTGDLGVMSDGELFIMGRIKDLLIVDGRNHYPDDIE
HHHHHHCCCEEECCCCCCCCCCEEEECCCCEECCCCEEEEECCCEEEEECCCCCCCCHHH
ATIQEITGGRVAAITVPDDLTEQLVAIIEYKEHGASAEDHMHKVHSVKREVTSAISKLHS
HHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LHVADLVLVSPGSIPITTSGKVRRSACVERYRSDGFKRLDVTV
HHHEEEEEECCCCEEEECCCCHHHHHHHHHHHCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]