Click here to switch to the map view.

The map label for this gene is glnG [H]

Identifier: 220916959

GI number: 220916959

Start: 2076819

End: 2078228

Strand: Direct

Name: glnG [H]

Synonym: A2cp1_1855

Alternate gene names: 220916959

Gene position: 2076819-2078228 (Clockwise)

Preceding gene: 220916958

Following gene: 220916960

Centisome position: 41.29

GC content: 72.27

Gene sequence:

>1410_bases
GTGAGCGAGTCGTTCTATCCCGCGTTCGCGATCCTGGCGGTGGACGACGAGGCCGCCTGGCTGCGCTCGGTCTCGCTCAC
GCTCGAGCGCGCGGCCGGCATCACCCACCTGCTCACCTGCCAGGACGCGCGCCGGGTGATGGAGGTGCTCGACGGCAACG
ACGTGGGCGTGGTGCTGCTGGACCTCACCATGCCCCACCTCTCCGGCGAGGACCTGCTCGGCCGCATCTCGGTGGAGCAC
CCCGACGTGAAGGTCATCGTGGTCTCCGGGATGAACCAGGTCGAGACCGCCGTGCGCTGCATGAAGCTGGGCGCGTTCGA
CTACCACGTGAAGACCGAGGAGGAGGACCGGCTGGTCGCCGGCGTCCTGCGCGCCGTGCGCATGCAGGAGCTGCAGCGCG
AGAACGGCGAGATGACCGCGCGCTTCATGTCCGGGGAGCTGCGCAACCCGGAGGCGTTCGCCGGCATCGTCACGCAGGAC
CGCACCATGCTGACGCTGTTCTCGTACGTGGAGGCGGTGGCGCGCAGCCCGCAGCCGCTGCTCGTCACCGGCGAGAGCGG
GGTCGGCAAGGAGCTGGTGGCCGGGGCGGCGCACCGGCTCTCCGGGCTGCCCGGGAAGCTGGTGGCGGTGAACGTGGCCG
GGCTCGACGACGCGGTGTTCGCCGACACGCTGTTCGGCCACGTGCGCGGCGCGTTCACCGGCGCCGAGCAGCCGCGGCGC
GGCATGGTGGAGGAGGCCGCCGACGGCACGCTGTTCCTCGACGAGATCGGGGACCTGTCGGTGCCGTCGCAGGTGAAGCT
GCTGCGCTTCCTGCAGGAGGGCGAGTTCTTCCCGCTCGGGAGCGACCGGCCGAAGCGGGTGAAGGTCCGCGTGGTCGCCG
CCACGCACCAGGACCTCGCCGCCAAGGAGCGCGCCGGGATGTTCCGGCGGGACCTGTACTACCGGCTGCGCACGCACCGC
GTGCGGGTGCCGCCGCTCCGGGAGCGCCGCGAGGACGTGCCGCTCCTGCTCGATCACTTCCTGGGCGAGGCCGCGCGGCA
GCTCGGCAAGAAGAAGCCCACCCCGCCGAAGCAGCTCGCGCAGCTGCTCATGACGCACGACTTCCCCGGCAACGTCCGCG
AGCTGCGCGCCATGTGCTTCGACGCGGTCAGCCGCCACCGCGACCGCGTGCTCTCCATGGACGCGTTCCTGGAGGCGATC
GGCCGGCACGGCAACGGCGCCCACCCCGCGGCGGAGGAGGGGCAGAACCCCTTCGGCGTGGTGGAGCGGCTGCCCACGTT
CGACGAGGCGCTGGAGCTGCTGGTGCAGGAGGCGATGCGGCGCTCCGGCGGGAACCAGACGCTCGCCGCGCGGCTGGTGG
GCATCTCGCAGCCCGCGCTCTCGAAGCGCCTGAAGTCCTACCGGCGCTGA

Upstream 100 bases:

>100_bases
TCGTGAAGGAGCACGGCGGCCGGCTCGAGTTCGGGTCGCAGCCCGGCGTCGGCACCACCGCGGCGCTGGTGCTGCCGGCG
CTGCGCGAGGAGGGCGCGGC

Downstream 100 bases:

>100_bases
TCCCGCGGAACGCGCAGCGGCCCGGCCGCGCCGCCGAACGGGCATTCCCGCGGGAATGTCCATGCCCGCTGGCATGGCAG
GGCCCAGACGCGCGGGATCA

Product: two component sigma-54 specific Fis family transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MSESFYPAFAILAVDDEAAWLRSVSLTLERAAGITHLLTCQDARRVMEVLDGNDVGVVLLDLTMPHLSGEDLLGRISVEH
PDVKVIVVSGMNQVETAVRCMKLGAFDYHVKTEEEDRLVAGVLRAVRMQELQRENGEMTARFMSGELRNPEAFAGIVTQD
RTMLTLFSYVEAVARSPQPLLVTGESGVGKELVAGAAHRLSGLPGKLVAVNVAGLDDAVFADTLFGHVRGAFTGAEQPRR
GMVEEAADGTLFLDEIGDLSVPSQVKLLRFLQEGEFFPLGSDRPKRVKVRVVAATHQDLAAKERAGMFRRDLYYRLRTHR
VRVPPLRERREDVPLLLDHFLGEAARQLGKKKPTPPKQLAQLLMTHDFPGNVRELRAMCFDAVSRHRDRVLSMDAFLEAI
GRHGNGAHPAAEEGQNPFGVVERLPTFDEALELLVQEAMRRSGGNQTLAARLVGISQPALSKRLKSYRR

Sequences:

>Translated_469_residues
MSESFYPAFAILAVDDEAAWLRSVSLTLERAAGITHLLTCQDARRVMEVLDGNDVGVVLLDLTMPHLSGEDLLGRISVEH
PDVKVIVVSGMNQVETAVRCMKLGAFDYHVKTEEEDRLVAGVLRAVRMQELQRENGEMTARFMSGELRNPEAFAGIVTQD
RTMLTLFSYVEAVARSPQPLLVTGESGVGKELVAGAAHRLSGLPGKLVAVNVAGLDDAVFADTLFGHVRGAFTGAEQPRR
GMVEEAADGTLFLDEIGDLSVPSQVKLLRFLQEGEFFPLGSDRPKRVKVRVVAATHQDLAAKERAGMFRRDLYYRLRTHR
VRVPPLRERREDVPLLLDHFLGEAARQLGKKKPTPPKQLAQLLMTHDFPGNVRELRAMCFDAVSRHRDRVLSMDAFLEAI
GRHGNGAHPAAEEGQNPFGVVERLPTFDEALELLVQEAMRRSGGNQTLAARLVGISQPALSKRLKSYRR
>Mature_468_residues
SESFYPAFAILAVDDEAAWLRSVSLTLERAAGITHLLTCQDARRVMEVLDGNDVGVVLLDLTMPHLSGEDLLGRISVEHP
DVKVIVVSGMNQVETAVRCMKLGAFDYHVKTEEEDRLVAGVLRAVRMQELQRENGEMTARFMSGELRNPEAFAGIVTQDR
TMLTLFSYVEAVARSPQPLLVTGESGVGKELVAGAAHRLSGLPGKLVAVNVAGLDDAVFADTLFGHVRGAFTGAEQPRRG
MVEEAADGTLFLDEIGDLSVPSQVKLLRFLQEGEFFPLGSDRPKRVKVRVVAATHQDLAAKERAGMFRRDLYYRLRTHRV
RVPPLRERREDVPLLLDHFLGEAARQLGKKKPTPPKQLAQLLMTHDFPGNVRELRAMCFDAVSRHRDRVLSMDAFLEAIG
RHGNGAHPAAEEGQNPFGVVERLPTFDEALELLVQEAMRRSGGNQTLAARLVGISQPALSKRLKSYRR

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=480, Percent_Identity=34.375, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1790437, Length=457, Percent_Identity=36.7614879649891, Blast_Score=234, Evalue=8e-63,
Organism=Escherichia coli, GI1788550, Length=458, Percent_Identity=33.4061135371179, Blast_Score=231, Evalue=6e-62,
Organism=Escherichia coli, GI1788905, Length=436, Percent_Identity=34.8623853211009, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1789087, Length=346, Percent_Identity=35.8381502890173, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI87082117, Length=266, Percent_Identity=42.1052631578947, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1789233, Length=322, Percent_Identity=36.0248447204969, Blast_Score=185, Evalue=6e-48,
Organism=Escherichia coli, GI87082152, Length=235, Percent_Identity=45.9574468085106, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI1786524, Length=497, Percent_Identity=30.9859154929577, Blast_Score=159, Evalue=4e-40,
Organism=Escherichia coli, GI87081872, Length=320, Percent_Identity=33.125, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1787583, Length=325, Percent_Identity=33.2307692307692, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1789828, Length=194, Percent_Identity=39.1752577319588, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI87081858, Length=337, Percent_Identity=29.3768545994065, Blast_Score=105, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 51851; Mature: 51720

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESFYPAFAILAVDDEAAWLRSVSLTLERAAGITHLLTCQDARRVMEVLDGNDVGVVLL
CCCCCCCEEEEEEECCHHHHHHHHHHHHHHHCCCHHEEEHHHHHHHHHHHCCCCCEEEEE
DLTMPHLSGEDLLGRISVEHPDVKVIVVSGMNQVETAVRCMKLGAFDYHVKTEEEDRLVA
EEECCCCCCCCHHEEEEECCCCEEEEEECCCHHHHHHHHHHHHCCEEEEECCCCHHHHHH
GVLRAVRMQELQRENGEMTARFMSGELRNPEAFAGIVTQDRTMLTLFSYVEAVARSPQPL
HHHHHHHHHHHHHCCCCEEEHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCE
LVTGESGVGKELVAGAAHRLSGLPGKLVAVNVAGLDDAVFADTLFGHVRGAFTGAEQPRR
EEECCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCC
GMVEEAADGTLFLDEIGDLSVPSQVKLLRFLQEGEFFPLGSDRPKRVKVRVVAATHQDLA
CCHHHHCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCHHHH
AKERAGMFRRDLYYRLRTHRVRVPPLRERREDVPLLLDHFLGEAARQLGKKKPTPPKQLA
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHH
QLLMTHDFPGNVRELRAMCFDAVSRHRDRVLSMDAFLEAIGRHGNGAHPAAEEGQNPFGV
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCHHH
VERLPTFDEALELLVQEAMRRSGGNQTLAARLVGISQPALSKRLKSYRR
HHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SESFYPAFAILAVDDEAAWLRSVSLTLERAAGITHLLTCQDARRVMEVLDGNDVGVVLL
CCCCCCEEEEEEECCHHHHHHHHHHHHHHHCCCHHEEEHHHHHHHHHHHCCCCCEEEEE
DLTMPHLSGEDLLGRISVEHPDVKVIVVSGMNQVETAVRCMKLGAFDYHVKTEEEDRLVA
EEECCCCCCCCHHEEEEECCCCEEEEEECCCHHHHHHHHHHHHCCEEEEECCCCHHHHHH
GVLRAVRMQELQRENGEMTARFMSGELRNPEAFAGIVTQDRTMLTLFSYVEAVARSPQPL
HHHHHHHHHHHHHCCCCEEEHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCE
LVTGESGVGKELVAGAAHRLSGLPGKLVAVNVAGLDDAVFADTLFGHVRGAFTGAEQPRR
EEECCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCC
GMVEEAADGTLFLDEIGDLSVPSQVKLLRFLQEGEFFPLGSDRPKRVKVRVVAATHQDLA
CCHHHHCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCHHHH
AKERAGMFRRDLYYRLRTHRVRVPPLRERREDVPLLLDHFLGEAARQLGKKKPTPPKQLA
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHH
QLLMTHDFPGNVRELRAMCFDAVSRHRDRVLSMDAFLEAIGRHGNGAHPAAEEGQNPFGV
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCHHH
VERLPTFDEALELLVQEAMRRSGGNQTLAARLVGISQPALSKRLKSYRR
HHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]