Click here to switch to the map view.

The map label for this gene is dctA

Identifier: 220916960

GI number: 220916960

Start: 2078564

End: 2079886

Strand: Direct

Name: dctA

Synonym: A2cp1_1856

Alternate gene names: 220916960

Gene position: 2078564-2079886 (Clockwise)

Preceding gene: 220916959

Following gene: 220916961

Centisome position: 41.33

GC content: 70.22

Gene sequence:

>1323_bases
GTGAAGCGTCTCGCGCGCAGCCTGTACCTGCAGGTGCTCCTGGCGGTCGTCCTCGGTGCGCTGGTCGGGCACCTGTTCCC
GGCCACCGGCGCGTCGCTGAAGCCGCTCGGCGACGGGTTCATCAAGCTGGTGAAGATGCTGATCGCGCCCATCGTGTTCG
CCACGGTGGTGACCGGCATCGCGAAGATGGGCGACCTGCGCAAGGTCGGGCGGGTGGGGCTGAAGGGGCTGCTCTACTTC
GAGGTGCTCACCACCGTCGCGCTCGCCATCGGCCTGGTGGTGGCGCGCCTGGCGCGCCCCGGCGCCGGGATGAACGTGGA
CCCGGCCACGCTCGACACCAAGGCCATCGCGTCCTACACGAACGGCGCGCAGGCCCACGGCACGGTGGACTTCCTCATGA
ACGTGATCCCGCGCGACGTGGCGGACGCGTTCGCGCGCGGCGACATCCTCCAGGTGCTGCTGTTCTCGGTGCTGTTCGGC
GCCGCGCTCGCCGCCCTGAAGGACAAGGGCCGCCCGGTGCTCGAGTTCGTGGACGGCCTGTCGCTGGTGCTGTTCCGCAT
CGTCGGGTTCGTGATGCGCCTCGCCCCGGTCGGCGCGTTCGGCGCCATGGCGTTCACGGTGGGGAAGTACGGCATCGCCA
CGCTGCTCAGCCTCGGCAAGCTCATCGCCTGCTTCTACGCGACCAGCGCGCTGTTCGTGGTGCTGATGCTGGGGCTGGTG
CTCCGCTGGTGCGGGCTGAGCCTGTTCCGGTTCCTGCGGTACATCAAGGAGGAGATCTTCGTGGTGCTCGGCACCTCCTC
CTCCGAGTCGGCCCTGCCGCTCATGATGCGGAAGATGGAGAAGCTCGGCTGCTCCAAGCCGGTGGTCGGGCTGGTGGTGC
CGATGGGCTACTCCTTCAACCTGGACGGCACGTCCATCTACCTGACGCTCGCCACCCTGTTCATCGCGCAGGCGACCAAC
ACGCACGTCACCCTGGTGCAGGAGCTGGAGATCCTGGCGGTGCTGCTGCTCACCTCGAAGGGGGCCGCGGCGGTGACGGG
CGGCGGCTTCATCACGCTGGCGGCGACGCTGTCCGCGGTGGGCAACATCCCGGTGGCGGGGCTGGCGCTGCTGCTCGGCG
TGGACCGCTTCATGTCCGAGGCCCGCGCCATCACCAACCTCATCGGGAATGGCGTCGCGTCGGTGGCGGTTTCGCGCTGG
GAGGGTGAGCTGGACCAGGCCCGCGCCCGCGCCGTCCTCGCCGGCACCGTGCCGGAGGAGGTGGAGCCGGCCAACGAGCC
CGAGCCGCCCGCGGTCCCGGCCGGTGCCGGGCTGCACGGCTGA

Upstream 100 bases:

>100_bases
GTGCTCTGGGGCGGGGCCTCGGCACACATGACCGGCCCGCGGCGCGACCGCCGGCCTCGACCCCGCGCCGGGCGGCGGCG
GCTCCCGGCAGGAGAGACAC

Downstream 100 bases:

>100_bases
GCCGCCGGGTCGCCCCCCCGCCGGACCCCACGGAGGAACCCGAGCATGATCCGCCTCATCCGCACGCTGGCCCGCCTCTG
GAACGCGCTCTGCGAGCTCG

Product: sodium:dicarboxylate symporter

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MKRLARSLYLQVLLAVVLGALVGHLFPATGASLKPLGDGFIKLVKMLIAPIVFATVVTGIAKMGDLRKVGRVGLKGLLYF
EVLTTVALAIGLVVARLARPGAGMNVDPATLDTKAIASYTNGAQAHGTVDFLMNVIPRDVADAFARGDILQVLLFSVLFG
AALAALKDKGRPVLEFVDGLSLVLFRIVGFVMRLAPVGAFGAMAFTVGKYGIATLLSLGKLIACFYATSALFVVLMLGLV
LRWCGLSLFRFLRYIKEEIFVVLGTSSSESALPLMMRKMEKLGCSKPVVGLVVPMGYSFNLDGTSIYLTLATLFIAQATN
THVTLVQELEILAVLLLTSKGAAAVTGGGFITLAATLSAVGNIPVAGLALLLGVDRFMSEARAITNLIGNGVASVAVSRW
EGELDQARARAVLAGTVPEEVEPANEPEPPAVPAGAGLHG

Sequences:

>Translated_440_residues
MKRLARSLYLQVLLAVVLGALVGHLFPATGASLKPLGDGFIKLVKMLIAPIVFATVVTGIAKMGDLRKVGRVGLKGLLYF
EVLTTVALAIGLVVARLARPGAGMNVDPATLDTKAIASYTNGAQAHGTVDFLMNVIPRDVADAFARGDILQVLLFSVLFG
AALAALKDKGRPVLEFVDGLSLVLFRIVGFVMRLAPVGAFGAMAFTVGKYGIATLLSLGKLIACFYATSALFVVLMLGLV
LRWCGLSLFRFLRYIKEEIFVVLGTSSSESALPLMMRKMEKLGCSKPVVGLVVPMGYSFNLDGTSIYLTLATLFIAQATN
THVTLVQELEILAVLLLTSKGAAAVTGGGFITLAATLSAVGNIPVAGLALLLGVDRFMSEARAITNLIGNGVASVAVSRW
EGELDQARARAVLAGTVPEEVEPANEPEPPAVPAGAGLHG
>Mature_440_residues
MKRLARSLYLQVLLAVVLGALVGHLFPATGASLKPLGDGFIKLVKMLIAPIVFATVVTGIAKMGDLRKVGRVGLKGLLYF
EVLTTVALAIGLVVARLARPGAGMNVDPATLDTKAIASYTNGAQAHGTVDFLMNVIPRDVADAFARGDILQVLLFSVLFG
AALAALKDKGRPVLEFVDGLSLVLFRIVGFVMRLAPVGAFGAMAFTVGKYGIATLLSLGKLIACFYATSALFVVLMLGLV
LRWCGLSLFRFLRYIKEEIFVVLGTSSSESALPLMMRKMEKLGCSKPVVGLVVPMGYSFNLDGTSIYLTLATLFIAQATN
THVTLVQELEILAVLLLTSKGAAAVTGGGFITLAATLSAVGNIPVAGLALLLGVDRFMSEARAITNLIGNGVASVAVSRW
EGELDQARARAVLAGTVPEEVEPANEPEPPAVPAGAGLHG

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family

Homologues:

Organism=Homo sapiens, GI4827012, Length=390, Percent_Identity=27.4358974358974, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI5032093, Length=339, Percent_Identity=28.9085545722714, Blast_Score=116, Evalue=5e-26,
Organism=Homo sapiens, GI21314632, Length=455, Percent_Identity=26.5934065934066, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI223468566, Length=296, Percent_Identity=30.4054054054054, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI223468564, Length=296, Percent_Identity=30.4054054054054, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI169790839, Length=491, Percent_Identity=23.8289205702648, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI40254478, Length=489, Percent_Identity=24.1308793456033, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI194239697, Length=463, Percent_Identity=23.110151187905, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI66773030, Length=407, Percent_Identity=24.5700245700246, Blast_Score=94, Evalue=4e-19,
Organism=Homo sapiens, GI301601644, Length=211, Percent_Identity=29.8578199052133, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI262359914, Length=402, Percent_Identity=22.8855721393035, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI1789947, Length=416, Percent_Identity=61.2980769230769, Blast_Score=531, Evalue=1e-152,
Organism=Escherichia coli, GI1790514, Length=423, Percent_Identity=39.4799054373522, Blast_Score=310, Evalue=9e-86,
Organism=Escherichia coli, GI1788024, Length=392, Percent_Identity=25.2551020408163, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1789473, Length=335, Percent_Identity=24.7761194029851, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17537407, Length=473, Percent_Identity=23.8900634249471, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI71983099, Length=475, Percent_Identity=25.6842105263158, Blast_Score=124, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI71983106, Length=475, Percent_Identity=25.6842105263158, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI193206505, Length=467, Percent_Identity=24.1970021413276, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI71996953, Length=425, Percent_Identity=23.5294117647059, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI193206654, Length=447, Percent_Identity=22.5950782997763, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17541374, Length=454, Percent_Identity=21.5859030837004, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24583025, Length=445, Percent_Identity=21.7977528089888, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI17137668, Length=445, Percent_Identity=21.7977528089888, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24583023, Length=445, Percent_Identity=21.7977528089888, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI281360483, Length=500, Percent_Identity=24.2, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI17137666, Length=500, Percent_Identity=24.2, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI281360481, Length=500, Percent_Identity=24.2, Blast_Score=95, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DCTA_ANAD2 (B8J708)

Other databases:

- EMBL:   CP001359
- RefSeq:   YP_002492264.1
- ProteinModelPortal:   B8J708
- SMR:   B8J708
- GeneID:   7296382
- GenomeReviews:   CP001359_GR
- KEGG:   acp:A2cp1_1856
- HOGENOM:   HBG739804
- ProtClustDB:   CLSK2311933
- HAMAP:   MF_01300
- InterPro:   IPR001991
- InterPro:   IPR018107
- PANTHER:   PTHR11958
- PRINTS:   PR00173

Pfam domain/function: PF00375 SDF

EC number: NA

Molecular weight: Translated: 46064; Mature: 46064

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1b0058dc)-; HASH(0x1c8c8988)-; HASH(0x1cbfa068)-; HASH(0x1c0d06d8)-; HASH(0x1b44e770)-; HASH(0x1cba83ec)-; HASH(0x1c60cad0)-; HASH(0x192a1790)-;

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCCCCCCCCCCCH
>Mature Secondary Structure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CCCCCCCCCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA