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The map label for this gene is hybA [H]

Identifier: 220915627

GI number: 220915627

Start: 584194

End: 585147

Strand: Reverse

Name: hybA [H]

Synonym: A2cp1_0508

Alternate gene names: 220915627

Gene position: 585147-584194 (Counterclockwise)

Preceding gene: 220915628

Following gene: 220915626

Centisome position: 11.63

GC content: 69.18

Gene sequence:

>954_bases
ATGACCATCTCTCGACGAGACCTGCTCAAGGGAGCGGTCGCCGCGGGAGCCGCCGTCGCCATCCCGGCCACGGCGGAGGC
CCGGCACGAGATCGAGCCGCTCCCCGACGCGGTGGGCATGCTGTTCGACTCGACGCTCTGCGTGGGCTGCCGCGCCTGCC
AGAGCGCCTGCAAGGCCTCGAACAAGCTCCCCGAGGACAAGGTGGAGGCGAACGGCGGCGTCTACGACGCGCCGAACGAC
CTCAACAGCACCACCAAGAACGTCATCAAGGCGTTCCCCGCCGGCGACGTGCAGCACTACATGAAGCAGCAGTGCATGCA
CTGCGTCGATCCCTCCTGCGTGTCCGTCTGCATGCTGGGCGCGCTGCACAAGGAGGGCGAGGGGAAGCGCGACTTCAAGG
GCGAGAAGAAGGGCACCGGCATCGTCCTCTGGGACAAGTGGACCTGCGTCGGCTGCCGCTACTGCCAGATCGGCTGCGCC
TTCAACGTCCCCAAGTTCGAGTGGTTCGAGGCGTTCCCGGCCATCGTGAAGTGCGAGCTGTGCCGGCACCGGCGCGACGA
GCAGAAGACCGGCCCGTACGCGGTCGCGAACCCCGCCTGCTGCGAGGTCTGCCCGCGCGAGGCGGTCGTCTTCGGCTACC
GCAAGGACCTGCTCGCCGAGGCGAAGCGGCGCGTCGCCGCCGACCCGCAGCGCTACAACCCGCACGTCTACGGCGAGCAC
GACGGCGGCGGCACGCAGGTGCTGTACCTCGCCGCGGCCGGGGTCCAGTTCCACGACCTCGGCCTGCCCGACCTCCCCGA
GCGCTCGCAGGCCGAGTTCTCGGAGAGCGTCTCGCACGCGCCGTACCTGCACGGCGTGACGCCGGTCGCGCTGTACGCGG
CGATGGCCTTCGTCATCAACCGCAACAAGAAGAAGGAAAAGGCCGCCGAGCACGGCGAGGAGGGCAAGCGATGA

Upstream 100 bases:

>100_bases
GCCTGGGCGGCGCGGTGATCGGCGCCCTGGTCGGCGCGGGCGTGGTGGCCTCCCGGAAGATGGCCGCCGCCGACGACGCA
GACGGGAAGGAGTAGCGGCG

Downstream 100 bases:

>100_bases
GCGGCCACGACCACCCGCAGGCCATCGGGGGGAAGATGCTCGACCCCCTCATGAAGATCCTGCTCGCCGTCTTCGGCGTC
TCCGCCGTGGTGATGGCGTA

Product: hydrogenase 2 protein HybA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MTISRRDLLKGAVAAGAAVAIPATAEARHEIEPLPDAVGMLFDSTLCVGCRACQSACKASNKLPEDKVEANGGVYDAPND
LNSTTKNVIKAFPAGDVQHYMKQQCMHCVDPSCVSVCMLGALHKEGEGKRDFKGEKKGTGIVLWDKWTCVGCRYCQIGCA
FNVPKFEWFEAFPAIVKCELCRHRRDEQKTGPYAVANPACCEVCPREAVVFGYRKDLLAEAKRRVAADPQRYNPHVYGEH
DGGGTQVLYLAAAGVQFHDLGLPDLPERSQAEFSESVSHAPYLHGVTPVALYAAMAFVINRNKKKEKAAEHGEEGKR

Sequences:

>Translated_317_residues
MTISRRDLLKGAVAAGAAVAIPATAEARHEIEPLPDAVGMLFDSTLCVGCRACQSACKASNKLPEDKVEANGGVYDAPND
LNSTTKNVIKAFPAGDVQHYMKQQCMHCVDPSCVSVCMLGALHKEGEGKRDFKGEKKGTGIVLWDKWTCVGCRYCQIGCA
FNVPKFEWFEAFPAIVKCELCRHRRDEQKTGPYAVANPACCEVCPREAVVFGYRKDLLAEAKRRVAADPQRYNPHVYGEH
DGGGTQVLYLAAAGVQFHDLGLPDLPERSQAEFSESVSHAPYLHGVTPVALYAAMAFVINRNKKKEKAAEHGEEGKR
>Mature_316_residues
TISRRDLLKGAVAAGAAVAIPATAEARHEIEPLPDAVGMLFDSTLCVGCRACQSACKASNKLPEDKVEANGGVYDAPNDL
NSTTKNVIKAFPAGDVQHYMKQQCMHCVDPSCVSVCMLGALHKEGEGKRDFKGEKKGTGIVLWDKWTCVGCRYCQIGCAF
NVPKFEWFEAFPAIVKCELCRHRRDEQKTGPYAVANPACCEVCPREAVVFGYRKDLLAEAKRRVAADPQRYNPHVYGEHD
GGGTQVLYLAAAGVQFHDLGLPDLPERSQAEFSESVSHAPYLHGVTPVALYAAMAFVINRNKKKEKAAEHGEEGKR

Specific function: Participates in the periplasmic electron-transferring activity of hydrogenase 2 during its catalytic turnover [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1789370, Length=339, Percent_Identity=38.0530973451327, Blast_Score=214, Evalue=8e-57,
Organism=Escherichia coli, GI1790326, Length=261, Percent_Identity=33.3333333333333, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1787749, Length=240, Percent_Identity=33.75, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI2367345, Length=237, Percent_Identity=32.0675105485232, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787122, Length=179, Percent_Identity=32.9608938547486, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1787872, Length=196, Percent_Identity=31.1224489795918, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI226510944, Length=254, Percent_Identity=25.5905511811024, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 34602; Mature: 34471

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
5.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTISRRDLLKGAVAAGAAVAIPATAEARHEIEPLPDAVGMLFDSTLCVGCRACQSACKAS
CCCCHHHHHHHHHHCCCEEEECCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
NKLPEDKVEANGGVYDAPNDLNSTTKNVIKAFPAGDVQHYMKQQCMHCVDPSCVSVCMLG
CCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHH
ALHKEGEGKRDFKGEKKGTGIVLWDKWTCVGCRYCQIGCAFNVPKFEWFEAFPAIVKCEL
HHHHCCCCCCCCCCCCCCCEEEEECCCEEECCEEEEECCCCCCCCHHHHHHHHHHHHHHH
CRHRRDEQKTGPYAVANPACCEVCPREAVVFGYRKDLLAEAKRRVAADPQRYNPHVYGEH
HHHCCCHHCCCCEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHCCCCEEECC
DGGGTQVLYLAAAGVQFHDLGLPDLPERSQAEFSESVSHAPYLHGVTPVALYAAMAFVIN
CCCCCEEEEEEECCCEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHC
RNKKKEKAAEHGEEGKR
CCCHHHHHHHCCCCCCC
>Mature Secondary Structure 
TISRRDLLKGAVAAGAAVAIPATAEARHEIEPLPDAVGMLFDSTLCVGCRACQSACKAS
CCCHHHHHHHHHHCCCEEEECCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
NKLPEDKVEANGGVYDAPNDLNSTTKNVIKAFPAGDVQHYMKQQCMHCVDPSCVSVCMLG
CCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHH
ALHKEGEGKRDFKGEKKGTGIVLWDKWTCVGCRYCQIGCAFNVPKFEWFEAFPAIVKCEL
HHHHCCCCCCCCCCCCCCCEEEEECCCEEECCEEEEECCCCCCCCHHHHHHHHHHHHHHH
CRHRRDEQKTGPYAVANPACCEVCPREAVVFGYRKDLLAEAKRRVAADPQRYNPHVYGEH
HHHCCCHHCCCCEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHCCCCEEECC
DGGGTQVLYLAAAGVQFHDLGLPDLPERSQAEFSESVSHAPYLHGVTPVALYAAMAFVIN
CCCCCEEEEEEECCCEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHC
RNKKKEKAAEHGEEGKR
CCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]