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The map label for this gene is hybO [H]

Identifier: 220915628

GI number: 220915628

Start: 585153

End: 586277

Strand: Reverse

Name: hybO [H]

Synonym: A2cp1_0509

Alternate gene names: 220915628

Gene position: 586277-585153 (Counterclockwise)

Preceding gene: 220915629

Following gene: 220915627

Centisome position: 11.66

GC content: 70.49

Gene sequence:

>1125_bases
ATGGACAATATCGGCTCCCAGATGATCAGCGGCCCGGAGACCAAGGGCGTCTCTCGCCGTGACTTCATCAAGGTGTGCAG
CGTCGCTGCCGCCGCCGTCGGCCTGCCCGCATGGGCCGGCGAGAAGATGGCGGAGAACGTCGGCAAGAAGAAGCCGTCGG
TCGTCTGGCTCCACTTCCAGGAGTGCACCGGCTGCACCGAGTCGCTCCTGCGGACCAGCCACCCGGACGTCGGCGCGCTG
ATCCTCGACCTCGTCTCGGTCGACTACCACGAGACCTTGTTCGCCGCGGCGGGCAAGCAGATCGAGGCGGCGCTCGAGAC
CGCGAAGAAGGCGGGCGGCTACGTGCTCGTGGTCGAGGGCGCGATCCCGAAGAAGGACGACGGCATCTACTGCATGGTCG
GTGGCAAGACCGCGGTGGACTCGCTGGTCGAGTGCGCCGAGAAGGCCGCGGCGGTGATCTGCATCGGCTCCTGCTCGAGC
TGGGGCGGCGTGCCCTCCGCGGCGCCGAACCCGACGCAGGCGGTGGGCGCCCCGGAGATCCTGAAGGAGAAGGGGATCAA
GAAGCCGGTGGTGACGCTGCCCGGCTGCCCGGCGAACCCGTACAACCTGCTCGGCACCGTGCTGCAGTTCGCGACGTTCG
GGACGCTGCCCAAGCTGGACGAGAAGGGCCGCCCGGCGTTCGCGTACGGCCGCGTGATCCACGAGGACTGCCCGCGGCGC
CCGCACTTCGACGCCGGCCGCTTCGCGCAGGCCTACGGCGACGAGGGCCACCGCAACGGGTGGTGCCTCTACAAGATCGG
CTGCAAGGGACCGCAGACGCACGCGAACTGCTCGCTGCTCTCGTTCTGCGAGGTGCCGGGCGCGTGGCCCATCGGCATCG
GCCACCCGTGCGTCGGCTGCACCGAGCAGGGCATCGTCTTCAACGTGCCCATCAACCAGAACCTGCCCATCGAGCACCCC
ACCGCCCCGATGGCCTACCCGGCCATCCACCCCGAGCACGGGGTGGTGGGCGGCAGCCCGGTCGCGGTCGGCCTGGGCGG
CGCGGTGATCGGCGCCCTGGTCGGCGCGGGCGTGGTGGCCTCCCGGAAGATGGCCGCCGCCGACGACGCAGACGGGAAGG
AGTAG

Upstream 100 bases:

>100_bases
CGCAAATCGTGCGCCGCCGGGAATCCCGTTTCCTTCCGCAATTTTGCCGCGTTGCAATTCCAATCCGGGGCGCTAGTCTC
GCGCGCACCCGGAGGACGGA

Downstream 100 bases:

>100_bases
CGGCGATGACCATCTCTCGACGAGACCTGCTCAAGGGAGCGGTCGCCGCGGGAGCCGCCGTCGCCATCCCGGCCACGGCG
GAGGCCCGGCACGAGATCGA

Product: hydrogenase (NiFe) small subunit HydA

Products: NA

Alternate protein names: HYD2; Membrane-bound hydrogenase 2 small subunit; NiFe hydrogenase [H]

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MDNIGSQMISGPETKGVSRRDFIKVCSVAAAAVGLPAWAGEKMAENVGKKKPSVVWLHFQECTGCTESLLRTSHPDVGAL
ILDLVSVDYHETLFAAAGKQIEAALETAKKAGGYVLVVEGAIPKKDDGIYCMVGGKTAVDSLVECAEKAAAVICIGSCSS
WGGVPSAAPNPTQAVGAPEILKEKGIKKPVVTLPGCPANPYNLLGTVLQFATFGTLPKLDEKGRPAFAYGRVIHEDCPRR
PHFDAGRFAQAYGDEGHRNGWCLYKIGCKGPQTHANCSLLSFCEVPGAWPIGIGHPCVGCTEQGIVFNVPINQNLPIEHP
TAPMAYPAIHPEHGVVGGSPVAVGLGGAVIGALVGAGVVASRKMAAADDADGKE

Sequences:

>Translated_374_residues
MDNIGSQMISGPETKGVSRRDFIKVCSVAAAAVGLPAWAGEKMAENVGKKKPSVVWLHFQECTGCTESLLRTSHPDVGAL
ILDLVSVDYHETLFAAAGKQIEAALETAKKAGGYVLVVEGAIPKKDDGIYCMVGGKTAVDSLVECAEKAAAVICIGSCSS
WGGVPSAAPNPTQAVGAPEILKEKGIKKPVVTLPGCPANPYNLLGTVLQFATFGTLPKLDEKGRPAFAYGRVIHEDCPRR
PHFDAGRFAQAYGDEGHRNGWCLYKIGCKGPQTHANCSLLSFCEVPGAWPIGIGHPCVGCTEQGIVFNVPINQNLPIEHP
TAPMAYPAIHPEHGVVGGSPVAVGLGGAVIGALVGAGVVASRKMAAADDADGKE
>Mature_374_residues
MDNIGSQMISGPETKGVSRRDFIKVCSVAAAAVGLPAWAGEKMAENVGKKKPSVVWLHFQECTGCTESLLRTSHPDVGAL
ILDLVSVDYHETLFAAAGKQIEAALETAKKAGGYVLVVEGAIPKKDDGIYCMVGGKTAVDSLVECAEKAAAVICIGSCSS
WGGVPSAAPNPTQAVGAPEILKEKGIKKPVVTLPGCPANPYNLLGTVLQFATFGTLPKLDEKGRPAFAYGRVIHEDCPRR
PHFDAGRFAQAYGDEGHRNGWCLYKIGCKGPQTHANCSLLSFCEVPGAWPIGIGHPCVGCTEQGIVFNVPINQNLPIEHP
TAPMAYPAIHPEHGVVGGSPVAVGLGGAVIGALVGAGVVASRKMAAADDADGKE

Specific function: This is one of three E.coli hydrogenases synthesized in response to different physiological conditions. HYD2 is involved in hydrogen uptake [H]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Periplasmic side. Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789371, Length=325, Percent_Identity=54.1538461538462, Blast_Score=348, Evalue=2e-97,
Organism=Escherichia coli, GI1787206, Length=294, Percent_Identity=42.8571428571429, Blast_Score=238, Evalue=5e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 38870; Mature: 38870

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
4.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNIGSQMISGPETKGVSRRDFIKVCSVAAAAVGLPAWAGEKMAENVGKKKPSVVWLHFQ
CCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCEEEEEEH
ECTGCTESLLRTSHPDVGALILDLVSVDYHETLFAAAGKQIEAALETAKKAGGYVLVVEG
HHCCHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEEC
AIPKKDDGIYCMVGGKTAVDSLVECAEKAAAVICIGSCSSWGGVPSAAPNPTQAVGAPEI
CCCCCCCCEEEEECCHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCCCCCCCHHCCCHHH
LKEKGIKKPVVTLPGCPANPYNLLGTVLQFATFGTLPKLDEKGRPAFAYGRVIHEDCPRR
HHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCC
PHFDAGRFAQAYGDEGHRNGWCLYKIGCKGPQTHANCSLLSFCEVPGAWPIGIGHPCVGC
CCCCCHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCC
TEQGIVFNVPINQNLPIEHPTAPMAYPAIHPEHGVVGGSPVAVGLGGAVIGALVGAGVVA
CCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHH
SRKMAAADDADGKE
HCCHHCCCCCCCCC
>Mature Secondary Structure
MDNIGSQMISGPETKGVSRRDFIKVCSVAAAAVGLPAWAGEKMAENVGKKKPSVVWLHFQ
CCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCEEEEEEH
ECTGCTESLLRTSHPDVGALILDLVSVDYHETLFAAAGKQIEAALETAKKAGGYVLVVEG
HHCCHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEEC
AIPKKDDGIYCMVGGKTAVDSLVECAEKAAAVICIGSCSSWGGVPSAAPNPTQAVGAPEI
CCCCCCCCEEEEECCHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCCCCCCCHHCCCHHH
LKEKGIKKPVVTLPGCPANPYNLLGTVLQFATFGTLPKLDEKGRPAFAYGRVIHEDCPRR
HHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCC
PHFDAGRFAQAYGDEGHRNGWCLYKIGCKGPQTHANCSLLSFCEVPGAWPIGIGHPCVGC
CCCCCHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCC
TEQGIVFNVPINQNLPIEHPTAPMAYPAIHPEHGVVGGSPVAVGLGGAVIGALVGAGVVA
CCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHH
SRKMAAADDADGKE
HCCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]