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The map label for this gene is hemN [H]

Identifier: 220905467

GI number: 220905467

Start: 2658863

End: 2660317

Strand: Reverse

Name: hemN [H]

Synonym: Ddes_2205

Alternate gene names: 220905467

Gene position: 2660317-2658863 (Counterclockwise)

Preceding gene: 220905471

Following gene: 220905460

Centisome position: 92.58

GC content: 63.78

Gene sequence:

>1455_bases
GTGCCGCATTTCCGCGCCATTTTTCTGTCACTGGAAAATCCTATGCTCGTATATATACATGTTCCCTTTTGCAGCACGCG
CTGCCGCTACTGTGCCTTTTATTCCAGCCCGTTGGGGCGCGGGGTGGACCCCGTGTCGTCTCCGGCCGTGCGCGACTATG
TGGACACCCTGTTTATGGAGCTTGCCCACTGGGGCGACCGGCTGGGCGGCAGCCCGGTGCAGACGGTATTTTTCGGCGGG
GGCACGCCAAGCCTTTTGCCGCCGCGCGTCATAGCTCTGGTTATGGAGCGCCTGGGGCGCTACTTCACGCTGGTTCCCAA
AGCCGAGGTGACGCTTGAGGCCAATCCGGAGTCCCTGCGCGGCGGGCACCGCGTGGCCCAGTATCTTGAGGCGGGCGTCA
ACCGTCTTTCCATTGGCATACAGAGCCTTGATGAAGGCATGCTGCGCATGCTGGGCCGTCCGCACAAGGCACAGGACAGC
CTGCATGCGGCCTTTCTTGCGCGCGAGGCCGGTTGCGCCAATATCAATGTGGATCTCATGTGGGGGCTGCCGGGGCAGAG
TGTGCGCCAATGGCTGCAAACGCTCAAGGATGTGATGCGCATGAGCCCGGACCATATTTCGGCCTACGGGCTGACTCTTG
AGCCGGGTACGCCGCTGGAGCTGGACGTGGAGGAAGGCCGCCTTATGCTGCCGCCGGAACGCGACCAGAACATCATGTTT
ATGGAGGGCGCGGCCCTGCTGGAACAGCACGGCTATCTGCACTACGAAATATCCAATTTTGCCCGCATGGGCTTTCAGTG
CCGTCACAATCTGGGCTACTGGGAAGGGGAGGACTACCTGGGCCTGGGACCTTCAGCCACGTCCACCATCGGCGGCCGCC
GATGGACCAATCCGGCCAGCCAGAAAGCCTGGGACGTGCGCACCCGCGAGGGCAGCCTCGGGCAGGATGTGGAGGAGCTG
ACACCGCAGACCAGAGTGCTGGAACTGCTCATGCTGCGCCTGCGCACGACGCGGGGGCTGCGGGTGAAGGCCTACCGGGA
AATGACCGGCAGGGATTTTGTGCGCGACCACCAGCGGCTGGTGCAGGCTTTGCATGAAAACGGGCTTATACGCATCCGCA
ACGGCTATCTGCGGCTGACGCGCAGCGGCATGCTGGTGTCCAACTCCATTCTGAGCAATCTTTTTGCCCGCACGCGTGAA
GTGCTCCGGCATGGGCTTGCCCCCGGGGCGCGCCCCGATGCGGTGCCATCCGCCGGGGTGGGCAGCGGCAGCCTGGGCGA
GGCCCTGCTGGCGGAAAACCGCGCGGCGCAAACATCGCCAGTGTCGCAGCCTGGGCAGGGTGAAGGACAGGGACCGGTGG
AAAATGTCATGGCCCCGGCCGGAGTGCTGGGCCACAAAAGAAAACCGGCCCGCAAGGAGCCGGAAATACGCGCCGTACAG
TGGCCCAGAGCCTGA

Upstream 100 bases:

>100_bases
GGCTTTTCGGCCAGTTGCGGGCCTGCATGGGGCTTGTCAGGAGGTGGTACTGCGGGCATGGTGGGGCATGTTCTTTGCAA
TAATGCCGTAAGGCGGCCCT

Downstream 100 bases:

>100_bases
CTGTACCGGGCCTGTGGATTGTGGAAATACAGCCTGAAGTGGCGGGGCCTGGGCGCTGGCAAGGCCTGCGCCGGGCTACG
GGCGTATCGGCGCGGAAAGA

Product: oxygen-independent coproporphyrinogen III oxidase

Products: NA

Alternate protein names: Coprogen oxidase; Coproporphyrinogenase [H]

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MPHFRAIFLSLENPMLVYIHVPFCSTRCRYCAFYSSPLGRGVDPVSSPAVRDYVDTLFMELAHWGDRLGGSPVQTVFFGG
GTPSLLPPRVIALVMERLGRYFTLVPKAEVTLEANPESLRGGHRVAQYLEAGVNRLSIGIQSLDEGMLRMLGRPHKAQDS
LHAAFLAREAGCANINVDLMWGLPGQSVRQWLQTLKDVMRMSPDHISAYGLTLEPGTPLELDVEEGRLMLPPERDQNIMF
MEGAALLEQHGYLHYEISNFARMGFQCRHNLGYWEGEDYLGLGPSATSTIGGRRWTNPASQKAWDVRTREGSLGQDVEEL
TPQTRVLELLMLRLRTTRGLRVKAYREMTGRDFVRDHQRLVQALHENGLIRIRNGYLRLTRSGMLVSNSILSNLFARTRE
VLRHGLAPGARPDAVPSAGVGSGSLGEALLAENRAAQTSPVSQPGQGEGQGPVENVMAPAGVLGHKRKPARKEPEIRAVQ
WPRA

Sequences:

>Translated_484_residues
MPHFRAIFLSLENPMLVYIHVPFCSTRCRYCAFYSSPLGRGVDPVSSPAVRDYVDTLFMELAHWGDRLGGSPVQTVFFGG
GTPSLLPPRVIALVMERLGRYFTLVPKAEVTLEANPESLRGGHRVAQYLEAGVNRLSIGIQSLDEGMLRMLGRPHKAQDS
LHAAFLAREAGCANINVDLMWGLPGQSVRQWLQTLKDVMRMSPDHISAYGLTLEPGTPLELDVEEGRLMLPPERDQNIMF
MEGAALLEQHGYLHYEISNFARMGFQCRHNLGYWEGEDYLGLGPSATSTIGGRRWTNPASQKAWDVRTREGSLGQDVEEL
TPQTRVLELLMLRLRTTRGLRVKAYREMTGRDFVRDHQRLVQALHENGLIRIRNGYLRLTRSGMLVSNSILSNLFARTRE
VLRHGLAPGARPDAVPSAGVGSGSLGEALLAENRAAQTSPVSQPGQGEGQGPVENVMAPAGVLGHKRKPARKEPEIRAVQ
WPRA
>Mature_483_residues
PHFRAIFLSLENPMLVYIHVPFCSTRCRYCAFYSSPLGRGVDPVSSPAVRDYVDTLFMELAHWGDRLGGSPVQTVFFGGG
TPSLLPPRVIALVMERLGRYFTLVPKAEVTLEANPESLRGGHRVAQYLEAGVNRLSIGIQSLDEGMLRMLGRPHKAQDSL
HAAFLAREAGCANINVDLMWGLPGQSVRQWLQTLKDVMRMSPDHISAYGLTLEPGTPLELDVEEGRLMLPPERDQNIMFM
EGAALLEQHGYLHYEISNFARMGFQCRHNLGYWEGEDYLGLGPSATSTIGGRRWTNPASQKAWDVRTREGSLGQDVEELT
PQTRVLELLMLRLRTTRGLRVKAYREMTGRDFVRDHQRLVQALHENGLIRIRNGYLRLTRSGMLVSNSILSNLFARTREV
LRHGLAPGARPDAVPSAGVGSGSLGEALLAENRAAQTSPVSQPGQGEGQGPVENVMAPAGVLGHKRKPARKEPEIRAVQW
PRA

Specific function: Anaerobic transformation of coproporphyrinogen-III into protoporphyrinogen-IX [H]

COG id: COG0635

COG function: function code H; Coproporphyrinogen III oxidase and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anaerobic coproporphyrinogen-III oxidase family [H]

Homologues:

Organism=Homo sapiens, GI8922911, Length=271, Percent_Identity=42.8044280442804, Blast_Score=196, Evalue=3e-50,
Organism=Escherichia coli, GI1789325, Length=391, Percent_Identity=33.7595907928389, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI87082341, Length=397, Percent_Identity=26.1964735516373, Blast_Score=121, Evalue=8e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR004559
- InterPro:   IPR010723
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06969 HemN_C; PF04055 Radical_SAM [H]

EC number: =1.3.99.22 [H]

Molecular weight: Translated: 53683; Mature: 53552

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHFRAIFLSLENPMLVYIHVPFCSTRCRYCAFYSSPLGRGVDPVSSPAVRDYVDTLFME
CCCCEEEEEEECCCEEEEEECCCCCCCHHHHEEECCCCCCCCCCCCCCHHHHHHHHHHHH
LAHWGDRLGGSPVQTVFFGGGTPSLLPPRVIALVMERLGRYFTLVPKAEVTLEANPESLR
HHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCEEEECCCCEEEEECCHHHHC
GGHRVAQYLEAGVNRLSIGIQSLDEGMLRMLGRPHKAQDSLHAAFLAREAGCANINVDLM
CHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEE
WGLPGQSVRQWLQTLKDVMRMSPDHISAYGLTLEPGTPLELDVEEGRLMLPPERDQNIMF
ECCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCEEEECCCCEEECCCCCCCCEEE
MEGAALLEQHGYLHYEISNFARMGFQCRHNLGYWEGEDYLGLGPSATSTIGGRRWTNPAS
ECCHHHHHHCCEEEEEHHHHHHHCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
QKAWDVRTREGSLGQDVEELTPQTRVLELLMLRLRTTRGLRVKAYREMTGRDFVRDHQRL
CCHHCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHH
VQALHENGLIRIRNGYLRLTRSGMLVSNSILSNLFARTREVLRHGLAPGARPDAVPSAGV
HHHHHHCCEEEEECCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
GSGSLGEALLAENRAAQTSPVSQPGQGEGQGPVENVMAPAGVLGHKRKPARKEPEIRAVQ
CCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHCCHHHHCCCCCCCCCCCCCEEEC
WPRA
CCCC
>Mature Secondary Structure 
PHFRAIFLSLENPMLVYIHVPFCSTRCRYCAFYSSPLGRGVDPVSSPAVRDYVDTLFME
CCCEEEEEEECCCEEEEEECCCCCCCHHHHEEECCCCCCCCCCCCCCHHHHHHHHHHHH
LAHWGDRLGGSPVQTVFFGGGTPSLLPPRVIALVMERLGRYFTLVPKAEVTLEANPESLR
HHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCEEEECCCCEEEEECCHHHHC
GGHRVAQYLEAGVNRLSIGIQSLDEGMLRMLGRPHKAQDSLHAAFLAREAGCANINVDLM
CHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEE
WGLPGQSVRQWLQTLKDVMRMSPDHISAYGLTLEPGTPLELDVEEGRLMLPPERDQNIMF
ECCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCEEEECCCCEEECCCCCCCCEEE
MEGAALLEQHGYLHYEISNFARMGFQCRHNLGYWEGEDYLGLGPSATSTIGGRRWTNPAS
ECCHHHHHHCCEEEEEHHHHHHHCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
QKAWDVRTREGSLGQDVEELTPQTRVLELLMLRLRTTRGLRVKAYREMTGRDFVRDHQRL
CCHHCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHH
VQALHENGLIRIRNGYLRLTRSGMLVSNSILSNLFARTREVLRHGLAPGARPDAVPSAGV
HHHHHHCCEEEEECCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
GSGSLGEALLAENRAAQTSPVSQPGQGEGQGPVENVMAPAGVLGHKRKPARKEPEIRAVQ
CCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHCCHHHHCCCCCCCCCCCCCEEEC
WPRA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8757728; 8969508; 9384377; 1339421 [H]