Click here to switch to the map view.

The map label for this gene is wcaL [H]

Identifier: 220905320

GI number: 220905320

Start: 2474497

End: 2475855

Strand: Reverse

Name: wcaL [H]

Synonym: Ddes_2058

Alternate gene names: 220905320

Gene position: 2475855-2474497 (Counterclockwise)

Preceding gene: 220905321

Following gene: 220905319

Centisome position: 86.16

GC content: 62.99

Gene sequence:

>1359_bases
ATGTCTTTTACCAGTGGCGATGCCACAGAAGCGGGAGCGGAACGCGGCCGGCCGGCAGCACCTCCGGCTGTCGCCTATGT
GCTTCTATGGTTCCCCTTATCCTCGGAGACCTTCATATTCCGTGAGGTTGTGCAGCTCAGGCAGCGGGGCCTTCCGGTCT
ATGCCTACAGTATGTACGGTGAAAACCTGAAGGGCTGCAGCGAAGAGATGCGCGATTATGACGGCCCTGTGCGGCGCATG
GGTTTCAGGGCTGCGGGCAGGGTTCTGGCCGCTTTCTGGCGCGCCTTGCGACAGCGGCCGGGCAAGGTGTGGGGGCTTAT
GCGCGAGGGGCTTTTCCGGCGCATGCGCAACCTTGAGGCCGTGGGCGAGAATACCTGGTGTTTTCTGGCGGGTTTTCTGC
TGGCCGAGCAGTGTCGCCGGGACGGCGTGCAGCTTATTCATGCGGCCTGGGCCAACGGGCCTGCCACTGCCGCCTGGGTG
GCCTCCCGTATGTCCGGTATTCCTTTTGCCTTTACGGGGCGTGCGGGTGATATCTATCCCGAAGACGGCCTGTTGCAGGA
AAAATCGCGCGATGCGCTCTTCATCCGCACCAATAACCGCACCAACGTGCAGTGGCTGCGCAGCTTTTGCCCGCCCGAAG
GCGGGGACAAGGTGCACGCCATCTATAACAGCCTCACATTCCGCCCGCGCGGGCGGTGTGCCATGCCCATGCAGCCGCCG
TACCGCCTGCTGGCCGTGGGGCGCTTTGCCCGTACCAAGGGCTTTCCCGAACTGCTCACGGCCATGGCCCGTCTGCGGCG
TGAAAACGTGCCGGTGACCCTGACCCTTGTGGGGGACGGCAACTGGCGGCGCAAGCTGACAAAAATGCGTGATGCGCTTG
GGCTTGCCGGTTTTGTGGACATGCCCGGCTTCATCCCCCACGATAAAATCTGCGACTTCATGCTGGATCACGACATGCTT
GTCATGCCCAGCGTTGTGCATACCAACGGCGACCGCGACGGCATCCCCAATGTGATCATGGAGGCCCTTTCACACCGCAT
GCCGGTTATTGCCACCGATGTGTGCGGTATTTCCGAGGTGATCATTGATGGTGAGACCGGCCTGCTGGTGCAGCAGCGCG
ATCCGCGCGCTCTGGCCGGGGCCGTGCGCCGCATGCTTGCCGACAGGCAGGCTGCGCTGGATATGGCCGAAGCGGGCCGC
GCCCTGGTGGAAAACCTTTTTGACCCCACCCGCAACATTACGGCCGTATACGACCTGTATGTAGCGGAATGCGCCCGCGC
CGCCCGGAAACGGGACAATGGTCACGATGCAGCCGGCCGGAGCTTTGCCGTAACCGGCATGGCGGAAGAGGGCGCATGA

Upstream 100 bases:

>100_bases
AAAATACAAAAACGACCACGCCGAAGAATCCCTGGGCGAATAAAAAATAACACTCCGGCCGGGCCTTATGGCCCGGCTCT
CGTTTGCTGCGGAGACCGAC

Downstream 100 bases:

>100_bases
GCCGTCCGGATCTTTATCTTGCCGTCAGCCTTGATGTGGAAGAAGAAGGGCTGTTCGGCGGGCAGTACGCCCGCCGGGGC
TGTTCCACTGCCAATACGGC

Product: group 1 glycosyl transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MSFTSGDATEAGAERGRPAAPPAVAYVLLWFPLSSETFIFREVVQLRQRGLPVYAYSMYGENLKGCSEEMRDYDGPVRRM
GFRAAGRVLAAFWRALRQRPGKVWGLMREGLFRRMRNLEAVGENTWCFLAGFLLAEQCRRDGVQLIHAAWANGPATAAWV
ASRMSGIPFAFTGRAGDIYPEDGLLQEKSRDALFIRTNNRTNVQWLRSFCPPEGGDKVHAIYNSLTFRPRGRCAMPMQPP
YRLLAVGRFARTKGFPELLTAMARLRRENVPVTLTLVGDGNWRRKLTKMRDALGLAGFVDMPGFIPHDKICDFMLDHDML
VMPSVVHTNGDRDGIPNVIMEALSHRMPVIATDVCGISEVIIDGETGLLVQQRDPRALAGAVRRMLADRQAALDMAEAGR
ALVENLFDPTRNITAVYDLYVAECARAARKRDNGHDAAGRSFAVTGMAEEGA

Sequences:

>Translated_452_residues
MSFTSGDATEAGAERGRPAAPPAVAYVLLWFPLSSETFIFREVVQLRQRGLPVYAYSMYGENLKGCSEEMRDYDGPVRRM
GFRAAGRVLAAFWRALRQRPGKVWGLMREGLFRRMRNLEAVGENTWCFLAGFLLAEQCRRDGVQLIHAAWANGPATAAWV
ASRMSGIPFAFTGRAGDIYPEDGLLQEKSRDALFIRTNNRTNVQWLRSFCPPEGGDKVHAIYNSLTFRPRGRCAMPMQPP
YRLLAVGRFARTKGFPELLTAMARLRRENVPVTLTLVGDGNWRRKLTKMRDALGLAGFVDMPGFIPHDKICDFMLDHDML
VMPSVVHTNGDRDGIPNVIMEALSHRMPVIATDVCGISEVIIDGETGLLVQQRDPRALAGAVRRMLADRQAALDMAEAGR
ALVENLFDPTRNITAVYDLYVAECARAARKRDNGHDAAGRSFAVTGMAEEGA
>Mature_451_residues
SFTSGDATEAGAERGRPAAPPAVAYVLLWFPLSSETFIFREVVQLRQRGLPVYAYSMYGENLKGCSEEMRDYDGPVRRMG
FRAAGRVLAAFWRALRQRPGKVWGLMREGLFRRMRNLEAVGENTWCFLAGFLLAEQCRRDGVQLIHAAWANGPATAAWVA
SRMSGIPFAFTGRAGDIYPEDGLLQEKSRDALFIRTNNRTNVQWLRSFCPPEGGDKVHAIYNSLTFRPRGRCAMPMQPPY
RLLAVGRFARTKGFPELLTAMARLRRENVPVTLTLVGDGNWRRKLTKMRDALGLAGFVDMPGFIPHDKICDFMLDHDMLV
MPSVVHTNGDRDGIPNVIMEALSHRMPVIATDVCGISEVIIDGETGLLVQQRDPRALAGAVRRMLADRQAALDMAEAGRA
LVENLFDPTRNITAVYDLYVAECARAARKRDNGHDAAGRSFAVTGMAEEGA

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1788357, Length=364, Percent_Identity=23.9010989010989, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296 [H]

Pfam domain/function: PF00534 Glycos_transf_1 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 50230; Mature: 50098

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFTSGDATEAGAERGRPAAPPAVAYVLLWFPLSSETFIFREVVQLRQRGLPVYAYSMYG
CCCCCCCCCHHHHHCCCCCCCHHHHHHHHEECCCCCHHHHHHHHHHHHCCCCEEEEECCC
ENLKGCSEEMRDYDGPVRRMGFRAAGRVLAAFWRALRQRPGKVWGLMREGLFRRMRNLEA
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
VGENTWCFLAGFLLAEQCRRDGVQLIHAAWANGPATAAWVASRMSGIPFAFTGRAGDIYP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCCCCCCC
EDGLLQEKSRDALFIRTNNRTNVQWLRSFCPPEGGDKVHAIYNSLTFRPRGRCAMPMQPP
CCCCCCCCCCCEEEEEECCCCHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCC
YRLLAVGRFARTKGFPELLTAMARLRRENVPVTLTLVGDGNWRRKLTKMRDALGLAGFVD
HHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHCCCCHHC
MPGFIPHDKICDFMLDHDMLVMPSVVHTNGDRDGIPNVIMEALSHRMPVIATDVCGISEV
CCCCCCHHHHHHHHHCCCCEECCHHHCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCE
IIDGETGLLVQQRDPRALAGAVRRMLADRQAALDMAEAGRALVENLFDPTRNITAVYDLY
EEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
VAECARAARKRDNGHDAAGRSFAVTGMAEEGA
HHHHHHHHHHCCCCCCCCCCEEEEECCCCCCC
>Mature Secondary Structure 
SFTSGDATEAGAERGRPAAPPAVAYVLLWFPLSSETFIFREVVQLRQRGLPVYAYSMYG
CCCCCCCCHHHHHCCCCCCCHHHHHHHHEECCCCCHHHHHHHHHHHHCCCCEEEEECCC
ENLKGCSEEMRDYDGPVRRMGFRAAGRVLAAFWRALRQRPGKVWGLMREGLFRRMRNLEA
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
VGENTWCFLAGFLLAEQCRRDGVQLIHAAWANGPATAAWVASRMSGIPFAFTGRAGDIYP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCCCCCCC
EDGLLQEKSRDALFIRTNNRTNVQWLRSFCPPEGGDKVHAIYNSLTFRPRGRCAMPMQPP
CCCCCCCCCCCEEEEEECCCCHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCC
YRLLAVGRFARTKGFPELLTAMARLRRENVPVTLTLVGDGNWRRKLTKMRDALGLAGFVD
HHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHCCCCHHC
MPGFIPHDKICDFMLDHDMLVMPSVVHTNGDRDGIPNVIMEALSHRMPVIATDVCGISEV
CCCCCCHHHHHHHHHCCCCEECCHHHCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCE
IIDGETGLLVQQRDPRALAGAVRRMLADRQAALDMAEAGRALVENLFDPTRNITAVYDLY
EEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
VAECARAARKRDNGHDAAGRSFAVTGMAEEGA
HHHHHHHHHHCCCCCCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609 [H]