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The map label for this gene is wcaL [H]

Identifier: 220905318

GI number: 220905318

Start: 2471820

End: 2473241

Strand: Reverse

Name: wcaL [H]

Synonym: Ddes_2056

Alternate gene names: 220905318

Gene position: 2473241-2471820 (Counterclockwise)

Preceding gene: 220905319

Following gene: 220905317

Centisome position: 86.07

GC content: 62.17

Gene sequence:

>1422_bases
ATGACAAAAGACACGACAAGCGGAAAACCCACCTCGCCGCCATTTGCGCCCGGCCTGCCCCATGTGGCCTGTGTGCTGCT
CTGGTACCCACTTTTCACCCAGCCTTTTATATTCCGTGAAGTGCAGGGCCTGCGTCAGCGCCTCCCCGTTTCGGTTTACA
GCCTGTATGGTCCCAACCTGCGCCACTGTTCTACGGAAATGCGCAGTGCGGCGCGAGAAACCATCACTATGGGTACGCGT
GCGCTACCGGCCATACTGGGTGAACTGCTACGCCAGATGGTCGCACATCCCTTGCGGCTGGGGCGGCTTTTCAGGCGCAC
CATGTTCCGCCGCTGGCGCTGCATTGAAACCCTGGGTGAAAATCTCTGGGCTTTTTGCGCCGGCGTCTATCTGGCTCGCC
GTTTCAAGGAGGCAGGCATAGACATGATTTATGCCCCCTGGCCGCGCGGTACAGCCACTGCCGCCTGGGTGGCCGGGAGC
CTTGCCGGACTGCCCTTTGCCACCTGCGCGCGGGGCGACAACCTTGAGCCTGCCGACCCGGACCTTGGTGACAAGCTGGA
GGCTGCCTTTCTTGTGCGCGCCAACAATGCCGCCGATAAGGGGCGTATTGAGAATTTCGACAGGGGACAGGCCAAGGGCA
AGGTGGAACTGGTCTATAACGGCCTGACCCTGCCGCCACCCCCGGGCAGGAGCGGATCGGCCGCTGAGGATGCGTCTGCG
GCCACCCCCGTACGCGACGGTGTGCACGGTTATGCCTGCGCGATGCCGCATCTGGAAGAAGCCCGGCCTTCAGCCGTACC
CGAACCCATGACGCGGCCTGTGCGTTTGCTGGCCCTGGGGCGTTTTGACGTTACCAAGGGTTTTGACGTACTGCTCCGTG
CCTGCGGCATTCTGCGCCGGCAGGGGCTGGACTTCAGGCTGACGCTGGCGGGCGGCGGCGGCAAGGCCATGGGCCTTGGC
TGCATGCAGGCAGAACTGATGCGTCTGTACAAGGAGCTGAAACTGGAAGGGTATGTGACCATGCCCGGTCTGATCTCGCA
TAACGAACTGCCCCGGCTTCTTGGTGAGCATGATATTTTTGCCGCTCCCTGCGTAGTGCACGCCTCGGGCCGCCGCGACG
GCATCCCCAATACGGTTATCGAGGCGCTGGCCTACGGCTTGCCCGTGGTCAGCACCACGGTCAACGCCCTGCCCGAGGTG
GTACGGCACGGCAAAACAGGTCTGGCCGTGCCTCCGGGAGATGCCGAAGCCCTGGCCGAAGCCATTGCCCTGCTTGCCGG
AGACACGGCCCTGGCGGCGCACCTGGCCCGCAACGGCCGGTTGCTGGCAGCCCATATGTTTGATCCTGAAGCCAACAATG
ATCGTTTGGCCCGCATATTTACCAATAATTACAAATGCTGGAAAGAATCATGTGTGGAATAG

Upstream 100 bases:

>100_bases
GCATCATGTGCGGCCTTAGGGCATTTTGTCTTTGAAACAAAGCGCATGCCCTGGAATACAACCTGTGGATTTGCCCCTTG
CAGACGCTCAGGAGCAAGTA

Downstream 100 bases:

>100_bases
CCGGTATCTGCCGATTGGACGCTTCGCCCCTTGATCCCCAGGCCCGCCACTGGGTGCAGGCCATGACCGACCGCATCGCC
CACCGGGGACCGGACGGCGA

Product: group 1 glycosyl transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MTKDTTSGKPTSPPFAPGLPHVACVLLWYPLFTQPFIFREVQGLRQRLPVSVYSLYGPNLRHCSTEMRSAARETITMGTR
ALPAILGELLRQMVAHPLRLGRLFRRTMFRRWRCIETLGENLWAFCAGVYLARRFKEAGIDMIYAPWPRGTATAAWVAGS
LAGLPFATCARGDNLEPADPDLGDKLEAAFLVRANNAADKGRIENFDRGQAKGKVELVYNGLTLPPPPGRSGSAAEDASA
ATPVRDGVHGYACAMPHLEEARPSAVPEPMTRPVRLLALGRFDVTKGFDVLLRACGILRRQGLDFRLTLAGGGGKAMGLG
CMQAELMRLYKELKLEGYVTMPGLISHNELPRLLGEHDIFAAPCVVHASGRRDGIPNTVIEALAYGLPVVSTTVNALPEV
VRHGKTGLAVPPGDAEALAEAIALLAGDTALAAHLARNGRLLAAHMFDPEANNDRLARIFTNNYKCWKESCVE

Sequences:

>Translated_473_residues
MTKDTTSGKPTSPPFAPGLPHVACVLLWYPLFTQPFIFREVQGLRQRLPVSVYSLYGPNLRHCSTEMRSAARETITMGTR
ALPAILGELLRQMVAHPLRLGRLFRRTMFRRWRCIETLGENLWAFCAGVYLARRFKEAGIDMIYAPWPRGTATAAWVAGS
LAGLPFATCARGDNLEPADPDLGDKLEAAFLVRANNAADKGRIENFDRGQAKGKVELVYNGLTLPPPPGRSGSAAEDASA
ATPVRDGVHGYACAMPHLEEARPSAVPEPMTRPVRLLALGRFDVTKGFDVLLRACGILRRQGLDFRLTLAGGGGKAMGLG
CMQAELMRLYKELKLEGYVTMPGLISHNELPRLLGEHDIFAAPCVVHASGRRDGIPNTVIEALAYGLPVVSTTVNALPEV
VRHGKTGLAVPPGDAEALAEAIALLAGDTALAAHLARNGRLLAAHMFDPEANNDRLARIFTNNYKCWKESCVE
>Mature_472_residues
TKDTTSGKPTSPPFAPGLPHVACVLLWYPLFTQPFIFREVQGLRQRLPVSVYSLYGPNLRHCSTEMRSAARETITMGTRA
LPAILGELLRQMVAHPLRLGRLFRRTMFRRWRCIETLGENLWAFCAGVYLARRFKEAGIDMIYAPWPRGTATAAWVAGSL
AGLPFATCARGDNLEPADPDLGDKLEAAFLVRANNAADKGRIENFDRGQAKGKVELVYNGLTLPPPPGRSGSAAEDASAA
TPVRDGVHGYACAMPHLEEARPSAVPEPMTRPVRLLALGRFDVTKGFDVLLRACGILRRQGLDFRLTLAGGGGKAMGLGC
MQAELMRLYKELKLEGYVTMPGLISHNELPRLLGEHDIFAAPCVVHASGRRDGIPNTVIEALAYGLPVVSTTVNALPEVV
RHGKTGLAVPPGDAEALAEAIALLAGDTALAAHLARNGRLLAAHMFDPEANNDRLARIFTNNYKCWKESCVE

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1788357, Length=199, Percent_Identity=34.1708542713568, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296 [H]

Pfam domain/function: PF00534 Glycos_transf_1 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 51364; Mature: 51233

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKDTTSGKPTSPPFAPGLPHVACVLLWYPLFTQPFIFREVQGLRQRLPVSVYSLYGPNL
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEHHCCCCH
RHCSTEMRSAARETITMGTRALPAILGELLRQMVAHPLRLGRLFRRTMFRRWRCIETLGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLWAFCAGVYLARRFKEAGIDMIYAPWPRGTATAAWVAGSLAGLPFATCARGDNLEPADP
HHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHCCCCHHHHCCCCCCCCCCC
DLGDKLEAAFLVRANNAADKGRIENFDRGQAKGKVELVYNGLTLPPPPGRSGSAAEDASA
CCCCHHCEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCC
ATPVRDGVHGYACAMPHLEEARPSAVPEPMTRPVRLLALGRFDVTKGFDVLLRACGILRR
CCCHHCCCCCHHCCCCCHHHCCCCCCCCCCCCCCHHEEECCCCCCCCHHHHHHHHHHHHH
QGLDFRLTLAGGGGKAMGLGCMQAELMRLYKELKLEGYVTMPGLISHNELPRLLGEHDIF
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHCCCCCE
AAPCVVHASGRRDGIPNTVIEALAYGLPVVSTTVNALPEVVRHGKTGLAVPPGDAEALAE
ECCEEEEECCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
AIALLAGDTALAAHLARNGRLLAAHMFDPEANNDRLARIFTNNYKCWKESCVE
HHHHHHCCHHHHHHHHHCCCEEEEEECCCCCCCCCEEEEHHCCCHHHHHHCCC
>Mature Secondary Structure 
TKDTTSGKPTSPPFAPGLPHVACVLLWYPLFTQPFIFREVQGLRQRLPVSVYSLYGPNL
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEHHCCCCH
RHCSTEMRSAARETITMGTRALPAILGELLRQMVAHPLRLGRLFRRTMFRRWRCIETLGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLWAFCAGVYLARRFKEAGIDMIYAPWPRGTATAAWVAGSLAGLPFATCARGDNLEPADP
HHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHCCCCHHHHCCCCCCCCCCC
DLGDKLEAAFLVRANNAADKGRIENFDRGQAKGKVELVYNGLTLPPPPGRSGSAAEDASA
CCCCHHCEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCC
ATPVRDGVHGYACAMPHLEEARPSAVPEPMTRPVRLLALGRFDVTKGFDVLLRACGILRR
CCCHHCCCCCHHCCCCCHHHCCCCCCCCCCCCCCHHEEECCCCCCCCHHHHHHHHHHHHH
QGLDFRLTLAGGGGKAMGLGCMQAELMRLYKELKLEGYVTMPGLISHNELPRLLGEHDIF
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHCCCCCE
AAPCVVHASGRRDGIPNTVIEALAYGLPVVSTTVNALPEVVRHGKTGLAVPPGDAEALAE
ECCEEEEECCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
AIALLAGDTALAAHLARNGRLLAAHMFDPEANNDRLARIFTNNYKCWKESCVE
HHHHHHCCHHHHHHHHHCCCEEEEEECCCCCCCCCEEEEHHCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609 [H]