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The map label for this gene is asnB [H]

Identifier: 220905317

GI number: 220905317

Start: 2469892

End: 2471832

Strand: Reverse

Name: asnB [H]

Synonym: Ddes_2055

Alternate gene names: 220905317

Gene position: 2471832-2469892 (Counterclockwise)

Preceding gene: 220905318

Following gene: 220905316

Centisome position: 86.02

GC content: 62.49

Gene sequence:

>1941_bases
ATGTGTGGAATAGCCGGTATCTGCCGATTGGACGCTTCGCCCCTTGATCCCCAGGCCCGCCACTGGGTGCAGGCCATGAC
CGACCGCATCGCCCACCGGGGACCGGACGGCGAAGGGCAGTGGCTGAACGGCCCGGTCTGCCTTGGGCACAGGCGGCTCT
CCATTATTGACCTCGGCACGGGCGGCCAGCCCATGCACAGTGTTGATGGGCGTTATGCCATCGTCTTCAATGGTGAAATA
TACAATTTTGCGGAACTGAAAGAAGAGCTTTCCAGCGCCGGGGCGTGCTTTCAGACCAGTTCGGATACAGAGGTCATCCT
TGAGGGCTACCGCCAGTGGGGCGTGGACTGCCTTGCCCGTTTTAACGGCATGTTTGCCTTTGCCCTGTGGGATGTTGTGG
AGCAGCGCCTTTTTTGCGCGCGTGATCCTTTCGGCAAGAAGCCCTTTTTTTATACGGTGCAGCAGGGCAGGCTGCATTTC
GCCTCGGAGCTGACGGCCCTTGAGCAACTGCGCGGCCAGGGCGCCGGGCTGAACCTGACCCTGCGGCCCGAAGCCGTCAT
GCGCTATCTGGCCTATGAATATGTGCCGACCCCGCAGACGGTGTATGGTGAGGTGGAAAGCCTGCCCCCGGCGCACTGGC
TTGTGCTTGAAGGCGGAAGCCTGCGCACCGGCCGTTACTGGGACATGCCCGTGCCGGACGAAAACGACCGCCGCAGCACC
GATGAACTTTGCGAAGAGCTGCGGGTTCTGCTGGCCCGGGCCGTGCGCCGCCGTATGGTCAGCGATGTGCCGCTGGGCGT
TTTTCTTTCGGGCGGCATTGACTCGTCCATTGTGGCAGGGCTGATGGCCCGGCAGTCGTCCTCGCCGGTAAAGACCTTTT
CCATAGGCTTTACCGAGGCCAGCTACGACGAGTCCCGCTATGCCCGTATTGTTGCCGGGACCTTCGGCACGGACCACCAC
GAGCGCGTACTTTCGGCAGAAGAATGCGCCGACAATCTGCCCGGCATCATCAGCAAAATGGACGTACCCATGGCGGACGC
TTCGGTTGCGCCCACCTGGCTGCTTTCGGGCGTGACCCGCGAAAAGGTCACTGTGGCGCTGGGGGGCGATGGCGCGGACG
AACTGTGGGCCGGGTATGAGCACTATATCGGCTTTAAGGTGGCGGAGTGGTATAACGCTTTGCCCGCCGTGCTGCGGCGC
AAGGTTGTGGAGCCTCTGGCCCGCCGCCTGCCGTCGTCCGCCGGATATATCAATCCGCGCCTTGCGGTAGCCACGTTTTT
GCGCGCCGCGCAGACTCCGGCCTGGCAGCGGGTGCAGACCATGCTGACGGCCTTTACGCCCGACATGCAGGCGGAGATAT
TGTCCGGCTCCTTCAGGGCGCAGGCTCCGGGATTTCTTGCGCCGGAACGCCTGTTTGCGCCCACGCGCGAACAGTATGAG
CACTGGCCCCATAACACGGCCGCGCCGCTGGCGAGAGCCTTTCATGTGTACGTGCGCCAGTTCATGCTGGATGACATTCT
GGTCAAGGTAGACCGTTGCTCCATGCTGCACAGCCTTGAGGTGCGCGCACCGTTTCTGGACAGGGATGTGGCCGAATTCG
CCGCCCGTCTGCCTGTGGACCGCAAACTGCACGGCTTCAAGCGCAAGTGGCTGCTCAAGCGGGCGTTTGCCGACCTGTTG
CCCAGAGAGATACTGTATCGCAACAAGCGGGGTTTTCAGATTCCCGTTGCTGAATGGCTGCGCGGACGCATGCGGCCGCT
TGTGGAAGACCTGCTCAGCGCAGACAGCCTCAGGGTGCAGGGTATTTTTGACCCTGCCGCCGTGCGCGCCCTTGTGGACG
AACATGTTTCGGGCAGGGCGGACCTGCGCAAGCCGCTCTGGACCCTGCTGGTCTTCCAGCTCTGGTGGCAGGCGCGCAAC
GGAAGCGGAACAGGTATATAG

Upstream 100 bases:

>100_bases
GCAACGGCCGGTTGCTGGCAGCCCATATGTTTGATCCTGAAGCCAACAATGATCGTTTGGCCCGCATATTTACCAATAAT
TACAAATGCTGGAAAGAATC

Downstream 100 bases:

>100_bases
ACAGCCGCCATTGGAAATGCTTTCATCATCGTTGTCGGGCCGCCTGCCCGGCGGCAATAGAAGGGCTGCCGGGGAGTGCC
CCCCGCCGGGGCCGCGCGGC

Product: asparagine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 646; Mature: 646

Protein sequence:

>646_residues
MCGIAGICRLDASPLDPQARHWVQAMTDRIAHRGPDGEGQWLNGPVCLGHRRLSIIDLGTGGQPMHSVDGRYAIVFNGEI
YNFAELKEELSSAGACFQTSSDTEVILEGYRQWGVDCLARFNGMFAFALWDVVEQRLFCARDPFGKKPFFYTVQQGRLHF
ASELTALEQLRGQGAGLNLTLRPEAVMRYLAYEYVPTPQTVYGEVESLPPAHWLVLEGGSLRTGRYWDMPVPDENDRRST
DELCEELRVLLARAVRRRMVSDVPLGVFLSGGIDSSIVAGLMARQSSSPVKTFSIGFTEASYDESRYARIVAGTFGTDHH
ERVLSAEECADNLPGIISKMDVPMADASVAPTWLLSGVTREKVTVALGGDGADELWAGYEHYIGFKVAEWYNALPAVLRR
KVVEPLARRLPSSAGYINPRLAVATFLRAAQTPAWQRVQTMLTAFTPDMQAEILSGSFRAQAPGFLAPERLFAPTREQYE
HWPHNTAAPLARAFHVYVRQFMLDDILVKVDRCSMLHSLEVRAPFLDRDVAEFAARLPVDRKLHGFKRKWLLKRAFADLL
PREILYRNKRGFQIPVAEWLRGRMRPLVEDLLSADSLRVQGIFDPAAVRALVDEHVSGRADLRKPLWTLLVFQLWWQARN
GSGTGI

Sequences:

>Translated_646_residues
MCGIAGICRLDASPLDPQARHWVQAMTDRIAHRGPDGEGQWLNGPVCLGHRRLSIIDLGTGGQPMHSVDGRYAIVFNGEI
YNFAELKEELSSAGACFQTSSDTEVILEGYRQWGVDCLARFNGMFAFALWDVVEQRLFCARDPFGKKPFFYTVQQGRLHF
ASELTALEQLRGQGAGLNLTLRPEAVMRYLAYEYVPTPQTVYGEVESLPPAHWLVLEGGSLRTGRYWDMPVPDENDRRST
DELCEELRVLLARAVRRRMVSDVPLGVFLSGGIDSSIVAGLMARQSSSPVKTFSIGFTEASYDESRYARIVAGTFGTDHH
ERVLSAEECADNLPGIISKMDVPMADASVAPTWLLSGVTREKVTVALGGDGADELWAGYEHYIGFKVAEWYNALPAVLRR
KVVEPLARRLPSSAGYINPRLAVATFLRAAQTPAWQRVQTMLTAFTPDMQAEILSGSFRAQAPGFLAPERLFAPTREQYE
HWPHNTAAPLARAFHVYVRQFMLDDILVKVDRCSMLHSLEVRAPFLDRDVAEFAARLPVDRKLHGFKRKWLLKRAFADLL
PREILYRNKRGFQIPVAEWLRGRMRPLVEDLLSADSLRVQGIFDPAAVRALVDEHVSGRADLRKPLWTLLVFQLWWQARN
GSGTGI
>Mature_646_residues
MCGIAGICRLDASPLDPQARHWVQAMTDRIAHRGPDGEGQWLNGPVCLGHRRLSIIDLGTGGQPMHSVDGRYAIVFNGEI
YNFAELKEELSSAGACFQTSSDTEVILEGYRQWGVDCLARFNGMFAFALWDVVEQRLFCARDPFGKKPFFYTVQQGRLHF
ASELTALEQLRGQGAGLNLTLRPEAVMRYLAYEYVPTPQTVYGEVESLPPAHWLVLEGGSLRTGRYWDMPVPDENDRRST
DELCEELRVLLARAVRRRMVSDVPLGVFLSGGIDSSIVAGLMARQSSSPVKTFSIGFTEASYDESRYARIVAGTFGTDHH
ERVLSAEECADNLPGIISKMDVPMADASVAPTWLLSGVTREKVTVALGGDGADELWAGYEHYIGFKVAEWYNALPAVLRR
KVVEPLARRLPSSAGYINPRLAVATFLRAAQTPAWQRVQTMLTAFTPDMQAEILSGSFRAQAPGFLAPERLFAPTREQYE
HWPHNTAAPLARAFHVYVRQFMLDDILVKVDRCSMLHSLEVRAPFLDRDVAEFAARLPVDRKLHGFKRKWLLKRAFADLL
PREILYRNKRGFQIPVAEWLRGRMRPLVEDLLSADSLRVQGIFDPAAVRALVDEHVSGRADLRKPLWTLLVFQLWWQARN
GSGTGI

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=414, Percent_Identity=29.2270531400966, Blast_Score=143, Evalue=6e-34,
Organism=Homo sapiens, GI168229252, Length=414, Percent_Identity=29.2270531400966, Blast_Score=143, Evalue=6e-34,
Organism=Homo sapiens, GI168229250, Length=414, Percent_Identity=29.2270531400966, Blast_Score=143, Evalue=6e-34,
Organism=Homo sapiens, GI296010848, Length=386, Percent_Identity=29.2746113989637, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI296010852, Length=314, Percent_Identity=28.343949044586, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI296010850, Length=314, Percent_Identity=28.343949044586, Blast_Score=108, Evalue=1e-23,
Organism=Escherichia coli, GI1786889, Length=402, Percent_Identity=30.3482587064677, Blast_Score=159, Evalue=7e-40,
Organism=Caenorhabditis elegans, GI71993933, Length=502, Percent_Identity=29.0836653386454, Blast_Score=154, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI25147557, Length=502, Percent_Identity=29.0836653386454, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI25147560, Length=357, Percent_Identity=31.9327731092437, Blast_Score=129, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6321563, Length=425, Percent_Identity=31.0588235294118, Blast_Score=166, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6325403, Length=422, Percent_Identity=29.6208530805687, Blast_Score=160, Evalue=6e-40,
Organism=Drosophila melanogaster, GI45553209, Length=424, Percent_Identity=31.3679245283019, Blast_Score=168, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 72432; Mature: 72432

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIAGICRLDASPLDPQARHWVQAMTDRIAHRGPDGEGQWLNGPVCLGHRRLSIIDLGT
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCEEEEEEECC
GGQPMHSVDGRYAIVFNGEIYNFAELKEELSSAGACFQTSSDTEVILEGYRQWGVDCLAR
CCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHH
FNGMFAFALWDVVEQRLFCARDPFGKKPFFYTVQQGRLHFASELTALEQLRGQGAGLNLT
HCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCEEE
LRPEAVMRYLAYEYVPTPQTVYGEVESLPPAHWLVLEGGSLRTGRYWDMPVPDENDRRST
ECHHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCEECCCCCCCCCCCCCH
DELCEELRVLLARAVRRRMVSDVPLGVFLSGGIDSSIVAGLMARQSSSPVKTFSIGFTEA
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCCCCCEEEEEECCCC
SYDESRYARIVAGTFGTDHHERVLSAEECADNLPGIISKMDVPMADASVAPTWLLSGVTR
CCCHHHHEEEEEECCCCCHHHHHHCHHHHHCCCCHHHHHCCCCCCCCCCCHHHHHCCCCC
EKVTVALGGDGADELWAGYEHYIGFKVAEWYNALPAVLRRKVVEPLARRLPSSAGYINPR
CEEEEEECCCCHHHHHHCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
LAVATFLRAAQTPAWQRVQTMLTAFTPDMQAEILSGSFRAQAPGFLAPERLFAPTREQYE
HHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCHHHCCCCHHHHH
HWPHNTAAPLARAFHVYVRQFMLDDILVKVDRCSMLHSLEVRAPFLDRDVAEFAARLPVD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCC
RKLHGFKRKWLLKRAFADLLPREILYRNKRGFQIPVAEWLRGRMRPLVEDLLSADSLRVQ
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEE
GIFDPAAVRALVDEHVSGRADLRKPLWTLLVFQLWWQARNGSGTGI
ECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MCGIAGICRLDASPLDPQARHWVQAMTDRIAHRGPDGEGQWLNGPVCLGHRRLSIIDLGT
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCEEEEEEECC
GGQPMHSVDGRYAIVFNGEIYNFAELKEELSSAGACFQTSSDTEVILEGYRQWGVDCLAR
CCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHH
FNGMFAFALWDVVEQRLFCARDPFGKKPFFYTVQQGRLHFASELTALEQLRGQGAGLNLT
HCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCEEE
LRPEAVMRYLAYEYVPTPQTVYGEVESLPPAHWLVLEGGSLRTGRYWDMPVPDENDRRST
ECHHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCEECCCCCCCCCCCCCH
DELCEELRVLLARAVRRRMVSDVPLGVFLSGGIDSSIVAGLMARQSSSPVKTFSIGFTEA
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCCCCCEEEEEECCCC
SYDESRYARIVAGTFGTDHHERVLSAEECADNLPGIISKMDVPMADASVAPTWLLSGVTR
CCCHHHHEEEEEECCCCCHHHHHHCHHHHHCCCCHHHHHCCCCCCCCCCCHHHHHCCCCC
EKVTVALGGDGADELWAGYEHYIGFKVAEWYNALPAVLRRKVVEPLARRLPSSAGYINPR
CEEEEEECCCCHHHHHHCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
LAVATFLRAAQTPAWQRVQTMLTAFTPDMQAEILSGSFRAQAPGFLAPERLFAPTREQYE
HHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCHHHCCCCHHHHH
HWPHNTAAPLARAFHVYVRQFMLDDILVKVDRCSMLHSLEVRAPFLDRDVAEFAARLPVD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCC
RKLHGFKRKWLLKRAFADLLPREILYRNKRGFQIPVAEWLRGRMRPLVEDLLSADSLRVQ
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEE
GIFDPAAVRALVDEHVSGRADLRKPLWTLLVFQLWWQARNGSGTGI
ECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]