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The map label for this gene is ytcB [H]

Identifier: 220905310

GI number: 220905310

Start: 2461306

End: 2462220

Strand: Reverse

Name: ytcB [H]

Synonym: Ddes_2048

Alternate gene names: 220905310

Gene position: 2462220-2461306 (Counterclockwise)

Preceding gene: 220905311

Following gene: 220905309

Centisome position: 85.69

GC content: 56.39

Gene sequence:

>915_bases
GTGAAAATAACCGTTTTTGGCGGATCCGGCTTTCTGGGTTCGCATATTTGCGACAAGCTTTCTGATGCCGGGCATGCTGT
CACCATTGTTGATCTGCATCCCTCTCCCTGGCTGCGACCTGATCAGGCCATGCTCACGGGCAATATTCTTGAAGAAGAAA
CCGTGCGCAGGGCTGTGGAAGGCGCAGACATGGTGTTCAACTACGCGGGCATAGCCGACATCGGCGAAGCCAATAACCGC
CCGGTGGACACAGCGCGCATCAATGTGCTCGGCAACGTCATGATTCTCGAAGCCTGCCGTCAGGCCGGGGTACAGCGCTA
TGTCTTTGCCAGTTCCCTGTACGTGTACGGCAAGTCGGGCGGTTTTTATCGTTGCAGCAAGCAGGCGTGCGAGCTGTATA
TTGAAAATTATCAGGCCATGCACAACCTGCCTTACACCATACTGCGGTACGGTTCGCTGTACGGTCCCCGGTCAGACCGG
CGTAACGCCATCAACCGCTTTGTGTACGAAGCTCTTTCCACAGGCGGTATTACCTATTATGGCGCGCCTACGGCCCTGCG
CGAATATGTGCATGTGGATGATGCCAGCGCCGCCACCCTGGCCGTGCTGGCTCCGGAATTTGAAAACCAGAACATCATCA
TTTCCGGCAACCAGCCCATGCGGGTCGGCGACCTGTTCAAGATGATCGGTGAAATGCTGGGCAAGGAACTGGTCATCAAT
TACCAGAACGACCCCAACAGCGGGCATTATCAGGTAACGCCCTATGCCTTCATGCCCAAGGTGGGGCGCAAGCTGGTGCC
GCCGCTTACCGTGGACCTCGGGCAGGGCATCCTGCGCGTTATGGAAGAAGAGCATAAGGAAATGCATCCGGATCTGGAGT
CAGAGGGCGGCTATCTCGTGGCTACAGACGACTAG

Upstream 100 bases:

>100_bases
TCGGGCGGCAGATCGCGGCAGGCAGCCGTTTTATTGCCTACGGCATTGATTCCGTCTTTCTCTGGCATGCTGCTGAGCGG
CCGGATCAGGGAGAATAACT

Downstream 100 bases:

>100_bases
GCGCTGTTTGCGCACGGCTTTTTTCTTTCGTTCGTACAGGCTTTCTCGCCAAAACGCCTGTCCGGTGAGCCATGCTGAAA
ATATTTCTGAAATTTTGAGG

Product: NAD-dependent epimerase/dehydratase

Products: UDPgalactose

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MKITVFGGSGFLGSHICDKLSDAGHAVTIVDLHPSPWLRPDQAMLTGNILEEETVRRAVEGADMVFNYAGIADIGEANNR
PVDTARINVLGNVMILEACRQAGVQRYVFASSLYVYGKSGGFYRCSKQACELYIENYQAMHNLPYTILRYGSLYGPRSDR
RNAINRFVYEALSTGGITYYGAPTALREYVHVDDASAATLAVLAPEFENQNIIISGNQPMRVGDLFKMIGEMLGKELVIN
YQNDPNSGHYQVTPYAFMPKVGRKLVPPLTVDLGQGILRVMEEEHKEMHPDLESEGGYLVATDD

Sequences:

>Translated_304_residues
MKITVFGGSGFLGSHICDKLSDAGHAVTIVDLHPSPWLRPDQAMLTGNILEEETVRRAVEGADMVFNYAGIADIGEANNR
PVDTARINVLGNVMILEACRQAGVQRYVFASSLYVYGKSGGFYRCSKQACELYIENYQAMHNLPYTILRYGSLYGPRSDR
RNAINRFVYEALSTGGITYYGAPTALREYVHVDDASAATLAVLAPEFENQNIIISGNQPMRVGDLFKMIGEMLGKELVIN
YQNDPNSGHYQVTPYAFMPKVGRKLVPPLTVDLGQGILRVMEEEHKEMHPDLESEGGYLVATDD
>Mature_304_residues
MKITVFGGSGFLGSHICDKLSDAGHAVTIVDLHPSPWLRPDQAMLTGNILEEETVRRAVEGADMVFNYAGIADIGEANNR
PVDTARINVLGNVMILEACRQAGVQRYVFASSLYVYGKSGGFYRCSKQACELYIENYQAMHNLPYTILRYGSLYGPRSDR
RNAINRFVYEALSTGGITYYGAPTALREYVHVDDASAATLAVLAPEFENQNIIISGNQPMRVGDLFKMIGEMLGKELVIN
YQNDPNSGHYQVTPYAFMPKVGRKLVPPLTVDLGQGILRVMEEEHKEMHPDLESEGGYLVATDD

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=276, Percent_Identity=21.7391304347826, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI1786974, Length=137, Percent_Identity=29.9270072992701, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI115532424, Length=267, Percent_Identity=26.2172284644195, Blast_Score=78, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17568069, Length=233, Percent_Identity=24.0343347639485, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2

Molecular weight: Translated: 33626; Mature: 33626

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITVFGGSGFLGSHICDKLSDAGHAVTIVDLHPSPWLRPDQAMLTGNILEEETVRRAVE
CEEEEECCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHHC
GADMVFNYAGIADIGEANNRPVDTARINVLGNVMILEACRQAGVQRYVFASSLYVYGKSG
CHHHEEEECCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCHHEEEEEEEEEEECCC
GFYRCSKQACELYIENYQAMHNLPYTILRYGSLYGPRSDRRNAINRFVYEALSTGGITYY
CEEEHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEEE
GAPTALREYVHVDDASAATLAVLAPEFENQNIIISGNQPMRVGDLFKMIGEMLGKELVIN
CCHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCEEEEE
YQNDPNSGHYQVTPYAFMPKVGRKLVPPLTVDLGQGILRVMEEEHKEMHPDLESEGGYLV
ECCCCCCCCEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
ATDD
EECC
>Mature Secondary Structure
MKITVFGGSGFLGSHICDKLSDAGHAVTIVDLHPSPWLRPDQAMLTGNILEEETVRRAVE
CEEEEECCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHHC
GADMVFNYAGIADIGEANNRPVDTARINVLGNVMILEACRQAGVQRYVFASSLYVYGKSG
CHHHEEEECCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCHHEEEEEEEEEEECCC
GFYRCSKQACELYIENYQAMHNLPYTILRYGSLYGPRSDRRNAINRFVYEALSTGGITYY
CEEEHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEEE
GAPTALREYVHVDDASAATLAVLAPEFENQNIIISGNQPMRVGDLFKMIGEMLGKELVIN
CCHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCEEEEE
YQNDPNSGHYQVTPYAFMPKVGRKLVPPLTVDLGQGILRVMEEEHKEMHPDLESEGGYLV
ECCCCCCCCEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
ATDD
EECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDP-glucose

Specific reaction: UDP-glucose = UDP-galactose

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]