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The map label for this gene is kdsB [H]

Identifier: 220905309

GI number: 220905309

Start: 2460418

End: 2461188

Strand: Reverse

Name: kdsB [H]

Synonym: Ddes_2047

Alternate gene names: 220905309

Gene position: 2461188-2460418 (Counterclockwise)

Preceding gene: 220905310

Following gene: 220905308

Centisome position: 85.65

GC content: 56.03

Gene sequence:

>771_bases
ATGAACATTATTGCCATTATTCCCGCGCGTATGGGGTCCAGCCGTTATCCCGGCAAGCCGCTTGCCCTCATCCACAACGT
ACCCATGGTGGGCCATGTGGCCTTTCGCACGGGCATGAGCAAAACGCTTTCTTCCACCTATGTGGCCACCTGCGATGAAA
TTATAGAAAATTACTGCAAGGATGCGGGCCTGCCCTGCGTCATGACCGGCGATCATCATGTGCGCTGTTCTACGCGCACG
GCCGAAGCCCTGCTCAAGATCGAGGCCGCCACCGGCAAAAAGGCCGACATTGTTGTTATGGTGCAGGGCGACGAGCCTAT
GGTGCGGCCCGAGATGATCGACGCCGCCGTGGCCCCCATGCTGGCTGATCCGTCCATCAACGTGACCAACCTCATGGCTG
ATATGGATACCCTTGAAGAATTTGAAGACCCCAATGAAGTCAAGGTGGTGGTGGACAAGTTCAACGATGCCCTCTACTTC
TCGCGCGAGCCGATTCCTTCGCGCAAAAAGGGATCGGACAAGGTGCCCATGCGCAAACAGGTCTGCATCATTCCTTTTCG
CCGCGACTATCTGCTGCGCTTTAACGAAATGGAAGAAAGCCCCCTGGAGATCTACGAATCCGTGGACATGATGCGTATCC
TTGAATACGGCGAAAAGGTGCGTATGGTTCCCACGGCCAGCCGCACCTGGAGCGTGGACACGCCCGAGGACCTCGTGCGC
GTCTGCCGCCTTATGGAAGGTGACGACCTTATGAAGGAATACAGCAAATAA

Upstream 100 bases:

>100_bases
CTTTTTTCTTTCGTTCGTACAGGCTTTCTCGCCAAAACGCCTGTCCGGTGAGCCATGCTGAAAATATTTCTGAAATTTTG
AGGCCATAAGGAGCCGCTAT

Downstream 100 bases:

>100_bases
TCATGGCTGCGCTCGAAAAAACGGGCATGACGCAAGAAGCGGGGCAGGCCGGCCGGGCCGGCCCTGCTGTACCCTCCGAA
TCCGCCGGTACCGCAGCGCC

Product: 3-deoxy-manno-octulosonate cytidylyltransferase

Products: NA

Alternate protein names: CMP-2-keto-3-deoxyoctulosonic acid synthase; CKS; CMP-KDO synthase [H]

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MNIIAIIPARMGSSRYPGKPLALIHNVPMVGHVAFRTGMSKTLSSTYVATCDEIIENYCKDAGLPCVMTGDHHVRCSTRT
AEALLKIEAATGKKADIVVMVQGDEPMVRPEMIDAAVAPMLADPSINVTNLMADMDTLEEFEDPNEVKVVVDKFNDALYF
SREPIPSRKKGSDKVPMRKQVCIIPFRRDYLLRFNEMEESPLEIYESVDMMRILEYGEKVRMVPTASRTWSVDTPEDLVR
VCRLMEGDDLMKEYSK

Sequences:

>Translated_256_residues
MNIIAIIPARMGSSRYPGKPLALIHNVPMVGHVAFRTGMSKTLSSTYVATCDEIIENYCKDAGLPCVMTGDHHVRCSTRT
AEALLKIEAATGKKADIVVMVQGDEPMVRPEMIDAAVAPMLADPSINVTNLMADMDTLEEFEDPNEVKVVVDKFNDALYF
SREPIPSRKKGSDKVPMRKQVCIIPFRRDYLLRFNEMEESPLEIYESVDMMRILEYGEKVRMVPTASRTWSVDTPEDLVR
VCRLMEGDDLMKEYSK
>Mature_256_residues
MNIIAIIPARMGSSRYPGKPLALIHNVPMVGHVAFRTGMSKTLSSTYVATCDEIIENYCKDAGLPCVMTGDHHVRCSTRT
AEALLKIEAATGKKADIVVMVQGDEPMVRPEMIDAAVAPMLADPSINVTNLMADMDTLEEFEDPNEVKVVVDKFNDALYF
SREPIPSRKKGSDKVPMRKQVCIIPFRRDYLLRFNEMEESPLEIYESVDMMRILEYGEKVRMVPTASRTWSVDTPEDLVR
VCRLMEGDDLMKEYSK

Specific function: Activates KDO (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in Gram-negative bacteria [H]

COG id: COG1212

COG function: function code M; CMP-2-keto-3-deoxyoctulosonic acid synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsB family [H]

Homologues:

Organism=Escherichia coli, GI1787147, Length=248, Percent_Identity=36.6935483870968, Blast_Score=123, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003329
- InterPro:   IPR004528 [H]

Pfam domain/function: PF02348 CTP_transf_3 [H]

EC number: =2.7.7.38 [H]

Molecular weight: Translated: 28859; Mature: 28859

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
7.0 %Met     (Translated Protein)
9.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
7.0 %Met     (Mature Protein)
9.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIIAIIPARMGSSRYPGKPLALIHNVPMVGHVAFRTGMSKTLSSTYVATCDEIIENYCK
CCEEEEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DAGLPCVMTGDHHVRCSTRTAEALLKIEAATGKKADIVVMVQGDEPMVRPEMIDAAVAPM
HCCCCEEEECCCEEEECCHHHHHHEEEECCCCCCCEEEEEEECCCCCCCHHHHHHHHHHH
LADPSINVTNLMADMDTLEEFEDPNEVKVVVDKFNDALYFSREPIPSRKKGSDKVPMRKQ
HCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCC
VCIIPFRRDYLLRFNEMEESPLEIYESVDMMRILEYGEKVRMVPTASRTWSVDTPEDLVR
EEEEEECHHHHHEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCEECCCCHHHHHH
VCRLMEGDDLMKEYSK
HHHHHCCHHHHHHHCC
>Mature Secondary Structure
MNIIAIIPARMGSSRYPGKPLALIHNVPMVGHVAFRTGMSKTLSSTYVATCDEIIENYCK
CCEEEEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DAGLPCVMTGDHHVRCSTRTAEALLKIEAATGKKADIVVMVQGDEPMVRPEMIDAAVAPM
HCCCCEEEECCCEEEECCHHHHHHEEEECCCCCCCEEEEEEECCCCCCCHHHHHHHHHHH
LADPSINVTNLMADMDTLEEFEDPNEVKVVVDKFNDALYFSREPIPSRKKGSDKVPMRKQ
HCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCC
VCIIPFRRDYLLRFNEMEESPLEIYESVDMMRILEYGEKVRMVPTASRTWSVDTPEDLVR
EEEEEECHHHHHEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCEECCCCHHHHHH
VCRLMEGDDLMKEYSK
HHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA