Click here to switch to the map view.

The map label for this gene is bphF [H]

Identifier: 220905311

GI number: 220905311

Start: 2462223

End: 2463026

Strand: Reverse

Name: bphF [H]

Synonym: Ddes_2049

Alternate gene names: 220905311

Gene position: 2463026-2462223 (Counterclockwise)

Preceding gene: 220905313

Following gene: 220905310

Centisome position: 85.72

GC content: 60.45

Gene sequence:

>804_bases
ATGCCGTTTGATATGACCGTACATGAAATACGCGCGCTTCTGGCTGCGGACAAAGCCACAGTAGGTACATGGCTGCAGCT
GCCTTCTGCGGATGTGGCGGAGCTTATGGCTCGCGCGGGCTATGACTGGGTGGCTGTGGATATGGAACACGGCTCCTTTG
GCCGCACCGGGCTGCCGGACATCTTCCGGGCCATCGAATGCGGTGGGGCTGCACCCTTTGCCCGCCTGCCCGAAGCCGGG
AAAACCCAGATCAAGTCCGCTCTGGAGGCGGGTGCTCAAGGGCTGATTTTTCCCATGATTGAAAGCCGTGAGCAACTGGA
CCGCGCCATTGACTGGGCCGTTTATCCCGGCCAGGACCATTGGCGTTCCGCCGGAGAAACCGCCGGTGAATACAGGGGAG
TGGGCTTCTGCCGGGCCAATGCATTCGGCAAATGCTTTGACGAGTATCGCGGCTCCCTCGCGCCCGAAATATTTCTTGTG
GCGCAGATAGAGCATATCCGCGCTGTGGAAAACCTGGATGCCATCCTGTCGCATCCGCGCCTGGACGCCATAATGGTCGG
TCCATACGACCTTTCCGGCTCCATGGGGCTTACGGGGCAGTTTGACCATAAGGACTTCAAGGCGGCTATGGAGCGCATCG
CGGCCGCCTGTGCGCGCCACAAGGCCCGCATGGGACTGCATATCGTGCAGCCCGACCCGGTTGAACTCGGGCGGCAGATC
GCGGCAGGCAGCCGTTTTATTGCCTACGGCATTGATTCCGTCTTTCTCTGGCATGCTGCTGAGCGGCCGGATCAGGGAGA
ATAA

Upstream 100 bases:

>100_bases
AATATGGGAGGGCCGGAGCAGACCCCTCTGGGCTGTATTGCGTGCCGCTGCGGCTGGGCGTATACTGGTGGTTCGGATGT
AACCTCAACCCTGCGGCGGC

Downstream 100 bases:

>100_bases
CTGTGAAAATAACCGTTTTTGGCGGATCCGGCTTTCTGGGTTCGCATATTTGCGACAAGCTTTCTGATGCCGGGCATGCT
GTCACCATTGTTGATCTGCA

Product: HpcH/HpaI aldolase

Products: NA

Alternate protein names: HOA [H]

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MPFDMTVHEIRALLAADKATVGTWLQLPSADVAELMARAGYDWVAVDMEHGSFGRTGLPDIFRAIECGGAAPFARLPEAG
KTQIKSALEAGAQGLIFPMIESREQLDRAIDWAVYPGQDHWRSAGETAGEYRGVGFCRANAFGKCFDEYRGSLAPEIFLV
AQIEHIRAVENLDAILSHPRLDAIMVGPYDLSGSMGLTGQFDHKDFKAAMERIAAACARHKARMGLHIVQPDPVELGRQI
AAGSRFIAYGIDSVFLWHAAERPDQGE

Sequences:

>Translated_267_residues
MPFDMTVHEIRALLAADKATVGTWLQLPSADVAELMARAGYDWVAVDMEHGSFGRTGLPDIFRAIECGGAAPFARLPEAG
KTQIKSALEAGAQGLIFPMIESREQLDRAIDWAVYPGQDHWRSAGETAGEYRGVGFCRANAFGKCFDEYRGSLAPEIFLV
AQIEHIRAVENLDAILSHPRLDAIMVGPYDLSGSMGLTGQFDHKDFKAAMERIAAACARHKARMGLHIVQPDPVELGRQI
AAGSRFIAYGIDSVFLWHAAERPDQGE
>Mature_266_residues
PFDMTVHEIRALLAADKATVGTWLQLPSADVAELMARAGYDWVAVDMEHGSFGRTGLPDIFRAIECGGAAPFARLPEAGK
TQIKSALEAGAQGLIFPMIESREQLDRAIDWAVYPGQDHWRSAGETAGEYRGVGFCRANAFGKCFDEYRGSLAPEIFLVA
QIEHIRAVENLDAILSHPRLDAIMVGPYDLSGSMGLTGQFDHKDFKAAMERIAAACARHKARMGLHIVQPDPVELGRQIA
AGSRFIAYGIDSVFLWHAAERPDQGE

Specific function: Catalyzes the reversible retro-aldol cleavage of 4- hydroxy-2-oxovalerate to pyruvate and acetaldehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1789514, Length=253, Percent_Identity=29.2490118577075, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1788578, Length=244, Percent_Identity=29.9180327868852, Blast_Score=104, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.3.39 [H]

Molecular weight: Translated: 29169; Mature: 29038

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFDMTVHEIRALLAADKATVGTWLQLPSADVAELMARAGYDWVAVDMEHGSFGRTGLPD
CCCCCHHHHHHHHHHHCCHHHHHHEECCCHHHHHHHHHCCCCEEEEEECCCCCCCCCHHH
IFRAIECGGAAPFARLPEAGKTQIKSALEAGAQGLIFPMIESREQLDRAIDWAVYPGQDH
HHHHHHCCCCCCHHHCCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCEECCCCHH
WRSAGETAGEYRGVGFCRANAFGKCFDEYRGSLAPEIFLVAQIEHIRAVENLDAILSHPR
HHHHCCCCHHHCCCEEEEHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCC
LDAIMVGPYDLSGSMGLTGQFDHKDFKAAMERIAAACARHKARMGLHIVQPDPVELGRQI
CCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHH
AAGSRFIAYGIDSVFLWHAAERPDQGE
HCCCEEEEECCCHHEEECCCCCCCCCC
>Mature Secondary Structure 
PFDMTVHEIRALLAADKATVGTWLQLPSADVAELMARAGYDWVAVDMEHGSFGRTGLPD
CCCCHHHHHHHHHHHCCHHHHHHEECCCHHHHHHHHHCCCCEEEEEECCCCCCCCCHHH
IFRAIECGGAAPFARLPEAGKTQIKSALEAGAQGLIFPMIESREQLDRAIDWAVYPGQDH
HHHHHHCCCCCCHHHCCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCEECCCCHH
WRSAGETAGEYRGVGFCRANAFGKCFDEYRGSLAPEIFLVAQIEHIRAVENLDAILSHPR
HHHHCCCCHHHCCCEEEEHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCC
LDAIMVGPYDLSGSMGLTGQFDHKDFKAAMERIAAACARHKARMGLHIVQPDPVELGRQI
CCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHH
AAGSRFIAYGIDSVFLWHAAERPDQGE
HCCCEEEEECCCHHEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10049392 [H]