| Definition | Desulfovibrio desulfuricans subsp. desulfuricans str. ATCC 27774 chromosome, complete genome. |
|---|---|
| Accession | NC_011883 |
| Length | 2,873,437 |
Click here to switch to the map view.
The map label for this gene is ligA
Identifier: 220904693
GI number: 220904693
Start: 1704347
End: 1706485
Strand: Reverse
Name: ligA
Synonym: Ddes_1425
Alternate gene names: 220904693
Gene position: 1706485-1704347 (Counterclockwise)
Preceding gene: 220904694
Following gene: 220904692
Centisome position: 59.39
GC content: 60.26
Gene sequence:
>2139_bases ATGGTACAGAAGAACGAACATCAGGGCGGGCAGTCGCAGCACAGCCTTTTTGCCGCCGGTCCCACGGGCGAAGAACGCCG GCGCGTCCAGTGGCTTACAGCCGAGCTTGAGCGTCATAATCACCTCTACCATACGCTGGACAGGCCCGAAATCAGTGATG ACCAGTTTGACGCCCTGTTTCGCGAATTGCAGGATCTGGAAACCCGCTGGCCTGAACTTCGCTCGCTCCATTCACCAACC TTGCGCGTGGGGGGCGGGCTGCTGGAAGGTCTTGCCAAAAAGGCCCACAGTCTTCAGATGTATGGTCTGGACAACGTATT TTCAGCCGGGCAGTGGCAGGAATTCGCGGAGCGTATGGCTCGCGCCTGGGGGGGAGATGTGAACGGTCCCCTGCCGGAAA GCTTCTGGTGTGACCCCAAGCTGGATGGCCTGGCTCTGGAGATCATCTATGCAGACGGCGTGTTGCAAGAAGCCCTTACC CGGGGAGACGGCGAGGTGGGTGAAGTTGTCACCGACGCTGTGCGTACCATCCGCACCGTACCCCTGCGACTTGCGGGGCC GGGGCCTTTTCCCGCCAGGCTTGAGGTGCGCGGCGAGGTTGTCATGTATAAAAATGATTTTGCCGTCCTTAATGAAAAAC AGGAAGCCCTTGGTCTGAAAACCTTTGCCAATCCCAGAAATGCTGCGGCAGGAACACTGCGCCAGCTGGACACTGCCATT ATCGGTTCACGCCCGTTGCGGTTTCTGGCTTACAGCCTTGGCCAGGCGCTTTGGACGCCCGCGCCCGTCTGTCTGCTGCA GTCTGAAGTCATGAGTCGGCTCAGGGAATACGGTTTTCTTACTCCGCCGGACGGCAGGCTGTGCAGCAGCGTGGCCGAGG TGGAGGAGTATGCCCAGTGGGTCAGGGAGCACCGCGCCGCATTCCCGATGGAAATTGACGGGGCTGTGGCCAAGCTGGAC AATCTTGAGGCCCAGCAGGCGCTTGGTTTTACCGCCCGGGCACCGCGTTTTGCCGTGGCCTTCAAGTTTCCGGCCGAACT GGCACAGACCCTGCTGAAAGACATTGAGATACAGGTGGGCCGCACGGGGGTGCTTACCCCTGTGGCCATGCTTGAGCCTG TCTCTGTGGGCGGCGTGATGGTCTCGCGTGCCACCCTGCATAATGAGGATGAAATACGCAATCGCGATGTGCGGGTGGGT GATACGGTCATGGTGCGCCGCGCAGGCGATGTCATTCCCGAGGTGGTAGGGCCGGTGCTGGAAAAGCGCCCGGAAAACGC CCGGGAATATGTTTTTCCCCACACTTGTCCGGCATGCGGCCAGCCTGCGTACCGGGAAGAGGGGGAAGCTGCCTGGCGGT GCGAAAACATGGCCTGTTCCGCTATCCGCCTGCGGGCCATCACGCATTTTGTGTCCAAGGCCGGTCTGGATATCGCGGGC GTGGGGCAGAAATGGATAGAACAGCTGGTCACCAGTGGCCGGGTGCAGTCTCCTGCTGATCTTTTTACTCTTACGGTGCA GGAACTGCTGGGCTTTGAGCGCATGGGAGAAGTGCTGGCCCACAAGTTTGTGGACGCGCTTGCCCGCGCCGTACATTCTG CCACCCTGCCGCGCCTCATCAGCGCTCTGGGTATAAGGCACGTGGGTGAACAGACCGCACGCACCCTGGCTTTGCATTTT GAAACGCTGGATGAACTGGAAAACGCCGGCGCGGAAACGCTGCTGAGTCTGCCGGATGTGGGACCGGAGGTGGCGTCATC CATCCATAACTTTTTCAACAGCCCCGCCAACCGAGAACAGCTTGAGCGCTTCAGGGCGCTTGGCCTGTGGCCGCGCGGCG GCCGGTCTGGCGGCGGTTCTTCCGGCAGCACAGGCGAGGGCGGTTTGGCGTCCGGGCCGCTGGCGGGAAAAAATATTCTT TTTACCGGAACCCTGAGCATGCCGCGCAGTGAAGCCGAAAAGCTGGCTGAAACCGCCGGTGCCACGCCACTTGGCGGGGT GAGCAAAAAACTTGACTACCTTGTGGCGGGGGAAAAGGCGGGCAGCAAGCTTGAAAAAGCACAGGCGCTGGGCGTAACGG TACTCACCGAAGAAGAATTTATGACCATGTTGCGCGAAGCCAAAGCGGCTTCGGAATAA
Upstream 100 bases:
>100_bases CAACTTGGCTGCGCGTTTACCACATCCCCAATGCTGTCGGTTTGCCAGCTTTTGCAACGATACATCCCCAATTTGACACG TTCCAGTATAAAGGTCTTTC
Downstream 100 bases:
>100_bases TTTGAATACAAAGCGAGCAAAGCATGAGTACAGCCATCATCGTGGTAGGGGCCAACGGACGCATGGGCAAAACCATCAGC CATCTGGCGGCGACGGAGTC
Product: DNA ligase, NAD-dependent
Products: NA
Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]
Number of amino acids: Translated: 712; Mature: 712
Protein sequence:
>712_residues MVQKNEHQGGQSQHSLFAAGPTGEERRRVQWLTAELERHNHLYHTLDRPEISDDQFDALFRELQDLETRWPELRSLHSPT LRVGGGLLEGLAKKAHSLQMYGLDNVFSAGQWQEFAERMARAWGGDVNGPLPESFWCDPKLDGLALEIIYADGVLQEALT RGDGEVGEVVTDAVRTIRTVPLRLAGPGPFPARLEVRGEVVMYKNDFAVLNEKQEALGLKTFANPRNAAAGTLRQLDTAI IGSRPLRFLAYSLGQALWTPAPVCLLQSEVMSRLREYGFLTPPDGRLCSSVAEVEEYAQWVREHRAAFPMEIDGAVAKLD NLEAQQALGFTARAPRFAVAFKFPAELAQTLLKDIEIQVGRTGVLTPVAMLEPVSVGGVMVSRATLHNEDEIRNRDVRVG DTVMVRRAGDVIPEVVGPVLEKRPENAREYVFPHTCPACGQPAYREEGEAAWRCENMACSAIRLRAITHFVSKAGLDIAG VGQKWIEQLVTSGRVQSPADLFTLTVQELLGFERMGEVLAHKFVDALARAVHSATLPRLISALGIRHVGEQTARTLALHF ETLDELENAGAETLLSLPDVGPEVASSIHNFFNSPANREQLERFRALGLWPRGGRSGGGSSGSTGEGGLASGPLAGKNIL FTGTLSMPRSEAEKLAETAGATPLGGVSKKLDYLVAGEKAGSKLEKAQALGVTVLTEEEFMTMLREAKAASE
Sequences:
>Translated_712_residues MVQKNEHQGGQSQHSLFAAGPTGEERRRVQWLTAELERHNHLYHTLDRPEISDDQFDALFRELQDLETRWPELRSLHSPT LRVGGGLLEGLAKKAHSLQMYGLDNVFSAGQWQEFAERMARAWGGDVNGPLPESFWCDPKLDGLALEIIYADGVLQEALT RGDGEVGEVVTDAVRTIRTVPLRLAGPGPFPARLEVRGEVVMYKNDFAVLNEKQEALGLKTFANPRNAAAGTLRQLDTAI IGSRPLRFLAYSLGQALWTPAPVCLLQSEVMSRLREYGFLTPPDGRLCSSVAEVEEYAQWVREHRAAFPMEIDGAVAKLD NLEAQQALGFTARAPRFAVAFKFPAELAQTLLKDIEIQVGRTGVLTPVAMLEPVSVGGVMVSRATLHNEDEIRNRDVRVG DTVMVRRAGDVIPEVVGPVLEKRPENAREYVFPHTCPACGQPAYREEGEAAWRCENMACSAIRLRAITHFVSKAGLDIAG VGQKWIEQLVTSGRVQSPADLFTLTVQELLGFERMGEVLAHKFVDALARAVHSATLPRLISALGIRHVGEQTARTLALHF ETLDELENAGAETLLSLPDVGPEVASSIHNFFNSPANREQLERFRALGLWPRGGRSGGGSSGSTGEGGLASGPLAGKNIL FTGTLSMPRSEAEKLAETAGATPLGGVSKKLDYLVAGEKAGSKLEKAQALGVTVLTEEEFMTMLREAKAASE >Mature_712_residues MVQKNEHQGGQSQHSLFAAGPTGEERRRVQWLTAELERHNHLYHTLDRPEISDDQFDALFRELQDLETRWPELRSLHSPT LRVGGGLLEGLAKKAHSLQMYGLDNVFSAGQWQEFAERMARAWGGDVNGPLPESFWCDPKLDGLALEIIYADGVLQEALT RGDGEVGEVVTDAVRTIRTVPLRLAGPGPFPARLEVRGEVVMYKNDFAVLNEKQEALGLKTFANPRNAAAGTLRQLDTAI IGSRPLRFLAYSLGQALWTPAPVCLLQSEVMSRLREYGFLTPPDGRLCSSVAEVEEYAQWVREHRAAFPMEIDGAVAKLD NLEAQQALGFTARAPRFAVAFKFPAELAQTLLKDIEIQVGRTGVLTPVAMLEPVSVGGVMVSRATLHNEDEIRNRDVRVG DTVMVRRAGDVIPEVVGPVLEKRPENAREYVFPHTCPACGQPAYREEGEAAWRCENMACSAIRLRAITHFVSKAGLDIAG VGQKWIEQLVTSGRVQSPADLFTLTVQELLGFERMGEVLAHKFVDALARAVHSATLPRLISALGIRHVGEQTARTLALHF ETLDELENAGAETLLSLPDVGPEVASSIHNFFNSPANREQLERFRALGLWPRGGRSGGGSSGSTGEGGLASGPLAGKNIL FTGTLSMPRSEAEKLAETAGATPLGGVSKKLDYLVAGEKAGSKLEKAQALGVTVLTEEEFMTMLREAKAASE
Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam
COG id: COG0272
COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 BRCT domain
Homologues:
Organism=Escherichia coli, GI1788750, Length=676, Percent_Identity=46.4497041420118, Blast_Score=548, Evalue=1e-157, Organism=Escherichia coli, GI87082305, Length=286, Percent_Identity=28.6713286713287, Blast_Score=84, Evalue=3e-17,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): DNLJ_DESDA (B8J0Q0)
Other databases:
- EMBL: CP001358 - RefSeq: YP_002480005.1 - ProteinModelPortal: B8J0Q0 - GeneID: 7285121 - GenomeReviews: CP001358_GR - KEGG: dds:Ddes_1425 - HOGENOM: HBG620317 - ProtClustDB: CLSK705331 - GO: GO:0005622 - HAMAP: MF_01588 - InterPro: IPR001357 - InterPro: IPR018239 - InterPro: IPR004150 - InterPro: IPR001679 - InterPro: IPR013839 - InterPro: IPR013840 - InterPro: IPR003583 - InterPro: IPR012340 - InterPro: IPR016027 - InterPro: IPR010994 - InterPro: IPR004149 - Gene3D: G3DSA:2.40.50.140 - PIRSF: PIRSF001604 - SMART: SM00292 - SMART: SM00278 - SMART: SM00532 - TIGRFAMs: TIGR00575
Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like
EC number: =6.5.1.2
Molecular weight: Translated: 77625; Mature: 77625
Theoretical pI: Translated: 5.57; Mature: 5.57
Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2
Important sites: ACT_SITE 140-140 BINDING 138-138 BINDING 161-161 BINDING 199-199 BINDING 318-318 BINDING 342-342
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.0 %Cys (Translated Protein) 2.0 %Met (Translated Protein) 2.9 %Cys+Met (Translated Protein) 1.0 %Cys (Mature Protein) 2.0 %Met (Mature Protein) 2.9 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MVQKNEHQGGQSQHSLFAAGPTGEERRRVQWLTAELERHNHLYHTLDRPEISDDQFDALF CCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCHHHHHHH RELQDLETRWPELRSLHSPTLRVGGGLLEGLAKKAHSLQMYGLDNVFSAGQWQEFAERMA HHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHEEEECCHHHHCCCCHHHHHHHHH RAWGGDVNGPLPESFWCDPKLDGLALEIIYADGVLQEALTRGDGEVGEVVTDAVRTIRTV HHCCCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHC PLRLAGPGPFPARLEVRGEVVMYKNDFAVLNEKQEALGLKTFANPRNAAAGTLRQLDTAI CEEECCCCCCCCEEEECCCEEEEECCHHHHCCHHHHHCHHHCCCCCCHHHHHHHHHHHHH IGSRPLRFLAYSLGQALWTPAPVCLLQSEVMSRLREYGFLTPPDGRLCSSVAEVEEYAQW HCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH VREHRAAFPMEIDGAVAKLDNLEAQQALGFTARAPRFAVAFKFPAELAQTLLKDIEIQVG HHHHHCCCCEECCHHHHHHHCHHHHHHCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHCC RTGVLTPVAMLEPVSVGGVMVSRATLHNEDEIRNRDVRVGDTVMVRRAGDVIPEVVGPVL CCCCCCHHHHHCCCCCCCCHHHHHHHCCCHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHH EKRPENAREYVFPHTCPACGQPAYREEGEAAWRCENMACSAIRLRAITHFVSKAGLDIAG HCCCCCHHHCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEC VGQKWIEQLVTSGRVQSPADLFTLTVQELLGFERMGEVLAHKFVDALARAVHSATLPRLI CCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH SALGIRHVGEQTARTLALHFETLDELENAGAETLLSLPDVGPEVASSIHNFFNSPANREQ HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHCCCCCHHH LERFRALGLWPRGGRSGGGSSGSTGEGGLASGPLAGKNILFTGTLSMPRSEAEKLAETAG HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCC ATPLGGVSKKLDYLVAGEKAGSKLEKAQALGVTVLTEEEFMTMLREAKAASE CCCCCCHHHHHHHHEECCHHCHHHHHHHHHCEEEEEHHHHHHHHHHHHHCCC >Mature Secondary Structure MVQKNEHQGGQSQHSLFAAGPTGEERRRVQWLTAELERHNHLYHTLDRPEISDDQFDALF CCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCHHHHHHH RELQDLETRWPELRSLHSPTLRVGGGLLEGLAKKAHSLQMYGLDNVFSAGQWQEFAERMA HHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHEEEECCHHHHCCCCHHHHHHHHH RAWGGDVNGPLPESFWCDPKLDGLALEIIYADGVLQEALTRGDGEVGEVVTDAVRTIRTV HHCCCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHC PLRLAGPGPFPARLEVRGEVVMYKNDFAVLNEKQEALGLKTFANPRNAAAGTLRQLDTAI CEEECCCCCCCCEEEECCCEEEEECCHHHHCCHHHHHCHHHCCCCCCHHHHHHHHHHHHH IGSRPLRFLAYSLGQALWTPAPVCLLQSEVMSRLREYGFLTPPDGRLCSSVAEVEEYAQW HCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH VREHRAAFPMEIDGAVAKLDNLEAQQALGFTARAPRFAVAFKFPAELAQTLLKDIEIQVG HHHHHCCCCEECCHHHHHHHCHHHHHHCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHCC RTGVLTPVAMLEPVSVGGVMVSRATLHNEDEIRNRDVRVGDTVMVRRAGDVIPEVVGPVL CCCCCCHHHHHCCCCCCCCHHHHHHHCCCHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHH EKRPENAREYVFPHTCPACGQPAYREEGEAAWRCENMACSAIRLRAITHFVSKAGLDIAG HCCCCCHHHCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEC VGQKWIEQLVTSGRVQSPADLFTLTVQELLGFERMGEVLAHKFVDALARAVHSATLPRLI CCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH SALGIRHVGEQTARTLALHFETLDELENAGAETLLSLPDVGPEVASSIHNFFNSPANREQ HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHCCCCCHHH LERFRALGLWPRGGRSGGGSSGSTGEGGLASGPLAGKNILFTGTLSMPRSEAEKLAETAG HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCC ATPLGGVSKKLDYLVAGEKAGSKLEKAQALGVTVLTEEEFMTMLREAKAASE CCCCCCHHHHHHHHEECCHHCHHHHHHHHHCEEEEEHHHHHHHHHHHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA