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The map label for this gene is lpdA [H]

Identifier: 220904684

GI number: 220904684

Start: 1694056

End: 1695441

Strand: Direct

Name: lpdA [H]

Synonym: Ddes_1416

Alternate gene names: 220904684

Gene position: 1694056-1695441 (Clockwise)

Preceding gene: 220904683

Following gene: 220904685

Centisome position: 58.96

GC content: 59.24

Gene sequence:

>1386_bases
GTGCAGTCTTTTGACGTGATCATCATAGGTGGAGGCCCGGGCGGAACCACGGCAGCCCGCATTCTGGCACAGGGCGGGAA
AAAAGTTGCGCTGATAGAAGACAAGCACTGGGGCGGCACATGCCTTAACTGCGGCTGCATCCCCACCAAGATGCTGCTTG
GCGCAGTGGCCCCCGGCAGCCTGCTGCGCGCGCAGGAGCGTCTGCGGGTTGTCAAAGGCAACGTGAAGGTAGACTTTACC
GCCCTGCAAGGCCGGGTCAGCCGATTCACCAAAGGGACAAGCCAAACACTTGCCAAAGCCCTTGCAGGATCGGGCGTTAC
ACTGTTTGAAGGACTCGGAACCTGCCTGGAACGCAAAGAAGGGCAGCCCCGTGTGCAGGTTGTCTGCGCCGAAAACCGGA
CAGACATGACCGCTCCCGATGTCATCCTGGCTTGCGGCACTGCATCAGCCGCTTTTCCCGGCCTTGCGCCCGACCACCAG
TGCGTACTGGACAGCACAGACCTGTTGCAGTCTGCTGCCATTCCCGAAAGCCTGATCATTGTCGGGGCCGGAGCCATTGG
CCTTGAAATGGCGGACTTTTTTTCAGCCATGGGCAGCAAGGTCACAGTAGTGGAGGCGGCTTCGCATGTTGCCCCCACTG
AAGACCCGGATATTGCCAAAGAGATGCTCCGTGCCCTGACCAAGAACGGCATAACCTGCCATGAGGGAACCAGGGCTAAA
GACCTGCGTACCGTTGACGGTAAAGCCCAGCTCAGCCTTGAGGACGGAACAGTCATCACGGCGGCCAAAGCCTTGGTAGC
CGTTGGCCGCAGCCCAAATACCGAAGGGCTTGGCGTGGAAAATCTGGGCGGAACAAAAACCCCCAGAGGATACGCCCAGG
TGGATGAAAACCTGCAATGCGCGTCCGGCCTGTATGCTGTGGGCGACATCAACGGCCTTACCCTGCTGGCCCACGCCGCC
GAACATCAGGCAGCCTATGTGGCCCGCCGTATTCTCGGCCAGGCAGACGGTCCCTATGAATCCGGACCTGTACCTTCCTG
TGTATATGGCGGCACGGAAATCATGCGTGTGGGGCATACTGCAACCGCCTTGCTGCAACAGGGCAAGTCCGTTGAAGTTT
CACAGGCCCCTCTTTCGATGAATCCCATCGCGCAGGCAAGCGGCGGTACTGCCGGCTTTGTAAAGGTTGTCTGGCATGAG
GGCCGCATGAAAGGCATCGCAGCCGTTGGGGCTGGCGTATCGCATCTGGTGACCGTGGCCCAGATGCTGCTCAAGGAAGG
CTACAGCGGGCACAGATTACACGAAATCATGTTTGCCCATCCCACGCTGGATGAAATTGTGCCCATGGCCATCAATGCGC
CCAGAAAGGCCGTTACGGCTGGATAA

Upstream 100 bases:

>100_bases
GCGTGGTCTGGACGCCCTGCCCGAGCAGAACATGCTCGTCTACCGTTCCGCCTCACGGCAGTTTCTTTTCAAAGCTGATA
TCGCGAAATAGGGGGCAGAC

Downstream 100 bases:

>100_bases
TCGCCTGCGGGCAAGGAGATATCATGAATTTTCCCACTGACAGAATTTACCACGCCGACCACCTCTGGGCTCAGGAACAG
GCGGACGGCTCCTGGCTGAT

Product: FAD-dependent pyridine nucleotide-disulfide oxidoreductase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MQSFDVIIIGGGPGGTTAARILAQGGKKVALIEDKHWGGTCLNCGCIPTKMLLGAVAPGSLLRAQERLRVVKGNVKVDFT
ALQGRVSRFTKGTSQTLAKALAGSGVTLFEGLGTCLERKEGQPRVQVVCAENRTDMTAPDVILACGTASAAFPGLAPDHQ
CVLDSTDLLQSAAIPESLIIVGAGAIGLEMADFFSAMGSKVTVVEAASHVAPTEDPDIAKEMLRALTKNGITCHEGTRAK
DLRTVDGKAQLSLEDGTVITAAKALVAVGRSPNTEGLGVENLGGTKTPRGYAQVDENLQCASGLYAVGDINGLTLLAHAA
EHQAAYVARRILGQADGPYESGPVPSCVYGGTEIMRVGHTATALLQQGKSVEVSQAPLSMNPIAQASGGTAGFVKVVWHE
GRMKGIAAVGAGVSHLVTVAQMLLKEGYSGHRLHEIMFAHPTLDEIVPMAINAPRKAVTAG

Sequences:

>Translated_461_residues
MQSFDVIIIGGGPGGTTAARILAQGGKKVALIEDKHWGGTCLNCGCIPTKMLLGAVAPGSLLRAQERLRVVKGNVKVDFT
ALQGRVSRFTKGTSQTLAKALAGSGVTLFEGLGTCLERKEGQPRVQVVCAENRTDMTAPDVILACGTASAAFPGLAPDHQ
CVLDSTDLLQSAAIPESLIIVGAGAIGLEMADFFSAMGSKVTVVEAASHVAPTEDPDIAKEMLRALTKNGITCHEGTRAK
DLRTVDGKAQLSLEDGTVITAAKALVAVGRSPNTEGLGVENLGGTKTPRGYAQVDENLQCASGLYAVGDINGLTLLAHAA
EHQAAYVARRILGQADGPYESGPVPSCVYGGTEIMRVGHTATALLQQGKSVEVSQAPLSMNPIAQASGGTAGFVKVVWHE
GRMKGIAAVGAGVSHLVTVAQMLLKEGYSGHRLHEIMFAHPTLDEIVPMAINAPRKAVTAG
>Mature_461_residues
MQSFDVIIIGGGPGGTTAARILAQGGKKVALIEDKHWGGTCLNCGCIPTKMLLGAVAPGSLLRAQERLRVVKGNVKVDFT
ALQGRVSRFTKGTSQTLAKALAGSGVTLFEGLGTCLERKEGQPRVQVVCAENRTDMTAPDVILACGTASAAFPGLAPDHQ
CVLDSTDLLQSAAIPESLIIVGAGAIGLEMADFFSAMGSKVTVVEAASHVAPTEDPDIAKEMLRALTKNGITCHEGTRAK
DLRTVDGKAQLSLEDGTVITAAKALVAVGRSPNTEGLGVENLGGTKTPRGYAQVDENLQCASGLYAVGDINGLTLLAHAA
EHQAAYVARRILGQADGPYESGPVPSCVYGGTEIMRVGHTATALLQQGKSVEVSQAPLSMNPIAQASGGTAGFVKVVWHE
GRMKGIAAVGAGVSHLVTVAQMLLKEGYSGHRLHEIMFAHPTLDEIVPMAINAPRKAVTAG

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=455, Percent_Identity=30.5494505494506, Blast_Score=182, Evalue=6e-46,
Organism=Homo sapiens, GI50301238, Length=459, Percent_Identity=24.6187363834423, Blast_Score=109, Evalue=7e-24,
Organism=Escherichia coli, GI1786307, Length=468, Percent_Identity=28.4188034188034, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI87081717, Length=455, Percent_Identity=27.9120879120879, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI87082354, Length=465, Percent_Identity=25.3763440860215, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1789915, Length=446, Percent_Identity=25.1121076233184, Blast_Score=106, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI32565766, Length=468, Percent_Identity=29.9145299145299, Blast_Score=178, Evalue=4e-45,
Organism=Caenorhabditis elegans, GI17557007, Length=476, Percent_Identity=25.2100840336134, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71983419, Length=392, Percent_Identity=26.0204081632653, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71983429, Length=392, Percent_Identity=26.0204081632653, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71982272, Length=398, Percent_Identity=24.1206030150754, Blast_Score=65, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6321091, Length=464, Percent_Identity=30.8189655172414, Blast_Score=177, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6325240, Length=480, Percent_Identity=26.25, Blast_Score=126, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6325166, Length=468, Percent_Identity=24.1452991452991, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21358499, Length=454, Percent_Identity=31.057268722467, Blast_Score=192, Evalue=3e-49,
Organism=Drosophila melanogaster, GI24640549, Length=475, Percent_Identity=25.0526315789474, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24640553, Length=475, Percent_Identity=25.0526315789474, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24640551, Length=475, Percent_Identity=25.0526315789474, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI17737741, Length=392, Percent_Identity=25.2551020408163, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR002218
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999 [H]

Pfam domain/function: PF01134 GIDA; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 47668; Mature: 47668

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: PS00626 RCC1_2 ; PS00076 PYRIDINE_REDOX_1 ; PS00599 AA_TRANSFER_CLASS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSFDVIIIGGGPGGTTAARILAQGGKKVALIEDKHWGGTCLNCGCIPTKMLLGAVAPGS
CCCEEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCCCCCEEECCCCHHHHHHHHCCCHH
LLRAQERLRVVKGNVKVDFTALQGRVSRFTKGTSQTLAKALAGSGVTLFEGLGTCLERKE
HHHHHHHHHEEECCCEEEEHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHCC
GQPRVQVVCAENRTDMTAPDVILACGTASAAFPGLAPDHQCVLDSTDLLQSAAIPESLII
CCCEEEEEEECCCCCCCCCCEEEEECCCHHCCCCCCCCCEEEECHHHHHHHHCCCCEEEE
VGAGAIGLEMADFFSAMGSKVTVVEAASHVAPTEDPDIAKEMLRALTKNGITCHEGTRAK
EECCHHHHHHHHHHHHCCCEEEEEEHHHHCCCCCCHHHHHHHHHHHHHCCCEECCCCCCC
DLRTVDGKAQLSLEDGTVITAAKALVAVGRSPNTEGLGVENLGGTKTPRGYAQVDENLQC
CEEECCCCEEEEECCCCEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCHH
ASGLYAVGDINGLTLLAHAAEHQAAYVARRILGQADGPYESGPVPSCVYGGTEIMRVGHT
HCCEEEEECCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCHHHEEECHH
ATALLQQGKSVEVSQAPLSMNPIAQASGGTAGFVKVVWHEGRMKGIAAVGAGVSHLVTVA
HHHHHHCCCCCEEECCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHCCHHHHHHHH
QMLLKEGYSGHRLHEIMFAHPTLDEIVPMAINAPRKAVTAG
HHHHHCCCCCCEEEEEEECCCCHHHHHHHHCCCCCHHCCCC
>Mature Secondary Structure
MQSFDVIIIGGGPGGTTAARILAQGGKKVALIEDKHWGGTCLNCGCIPTKMLLGAVAPGS
CCCEEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCCCCCEEECCCCHHHHHHHHCCCHH
LLRAQERLRVVKGNVKVDFTALQGRVSRFTKGTSQTLAKALAGSGVTLFEGLGTCLERKE
HHHHHHHHHEEECCCEEEEHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHCC
GQPRVQVVCAENRTDMTAPDVILACGTASAAFPGLAPDHQCVLDSTDLLQSAAIPESLII
CCCEEEEEEECCCCCCCCCCEEEEECCCHHCCCCCCCCCEEEECHHHHHHHHCCCCEEEE
VGAGAIGLEMADFFSAMGSKVTVVEAASHVAPTEDPDIAKEMLRALTKNGITCHEGTRAK
EECCHHHHHHHHHHHHCCCEEEEEEHHHHCCCCCCHHHHHHHHHHHHHCCCEECCCCCCC
DLRTVDGKAQLSLEDGTVITAAKALVAVGRSPNTEGLGVENLGGTKTPRGYAQVDENLQC
CEEECCCCEEEEECCCCEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCHH
ASGLYAVGDINGLTLLAHAAEHQAAYVARRILGQADGPYESGPVPSCVYGGTEIMRVGHT
HCCEEEEECCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCHHHEEECHH
ATALLQQGKSVEVSQAPLSMNPIAQASGGTAGFVKVVWHEGRMKGIAAVGAGVSHLVTVA
HHHHHHCCCCCEEECCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHCCHHHHHHHH
QMLLKEGYSGHRLHEIMFAHPTLDEIVPMAINAPRKAVTAG
HHHHHCCCCCCEEEEEEECCCCHHHHHHHHCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935 [H]