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The map label for this gene is gcvH [H]

Identifier: 220904685

GI number: 220904685

Start: 1695465

End: 1695845

Strand: Direct

Name: gcvH [H]

Synonym: Ddes_1417

Alternate gene names: 220904685

Gene position: 1695465-1695845 (Clockwise)

Preceding gene: 220904684

Following gene: 220904687

Centisome position: 59.0

GC content: 57.74

Gene sequence:

>381_bases
ATGAATTTTCCCACTGACAGAATTTACCACGCCGACCACCTCTGGGCTCAGGAACAGGCGGACGGCTCCTGGCTGATCGG
CATTACCGACTATGCTCAGGATCAGCTCGGCAGCGTCATCTTTGTGGACATGCCGGAACAGGGAACCCAATTTGAACAGG
GCCAATCCTGCGCGTCCATTGAGTCAGTAAAAGTGACCAGCGAGGCGATCATGCCCATTTCCGGCACAGTGCGCGCCATC
AACGATGCCCTTGCTGAGACGCCTGAGCTGCTTAATACCGCCCCCTACGGCGAAGGATGGCTCATACGCGTCACGGCCTT
GCCCTACGATGCGGCAGCCTGCATGAACAGTGACGCTTATGCGGCCGTGATCGGCGCATAA

Upstream 100 bases:

>100_bases
TGTTTGCCCATCCCACGCTGGATGAAATTGTGCCCATGGCCATCAATGCGCCCAGAAAGGCCGTTACGGCTGGATAATCG
CCTGCGGGCAAGGAGATATC

Downstream 100 bases:

>100_bases
GAACCCCCTGTTCCGACGGTCCGGAAGGTCCTCGCCAGGCTGCAAAATCCCCGACAGTTCATCTGCCGGGGATTTTTACA
TGTAAAAACAGTCTTTCCTT

Product: glycine cleavage system H protein

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 126; Mature: 126

Protein sequence:

>126_residues
MNFPTDRIYHADHLWAQEQADGSWLIGITDYAQDQLGSVIFVDMPEQGTQFEQGQSCASIESVKVTSEAIMPISGTVRAI
NDALAETPELLNTAPYGEGWLIRVTALPYDAAACMNSDAYAAVIGA

Sequences:

>Translated_126_residues
MNFPTDRIYHADHLWAQEQADGSWLIGITDYAQDQLGSVIFVDMPEQGTQFEQGQSCASIESVKVTSEAIMPISGTVRAI
NDALAETPELLNTAPYGEGWLIRVTALPYDAAACMNSDAYAAVIGA
>Mature_126_residues
MNFPTDRIYHADHLWAQEQADGSWLIGITDYAQDQLGSVIFVDMPEQGTQFEQGQSCASIESVKVTSEAIMPISGTVRAI
NDALAETPELLNTAPYGEGWLIRVTALPYDAAACMNSDAYAAVIGA

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=121, Percent_Identity=37.1900826446281, Blast_Score=87, Evalue=2e-18,
Organism=Homo sapiens, GI89057342, Length=119, Percent_Identity=36.9747899159664, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1789271, Length=105, Percent_Identity=43.8095238095238, Blast_Score=112, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI17507493, Length=116, Percent_Identity=37.9310344827586, Blast_Score=92, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17551294, Length=99, Percent_Identity=39.3939393939394, Blast_Score=86, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6319272, Length=96, Percent_Identity=44.7916666666667, Blast_Score=92, Evalue=2e-20,
Organism=Drosophila melanogaster, GI17865652, Length=122, Percent_Identity=36.8852459016393, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 13598; Mature: 13598

Theoretical pI: Translated: 3.77; Mature: 3.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFPTDRIYHADHLWAQEQADGSWLIGITDYAQDQLGSVIFVDMPEQGTQFEQGQSCASI
CCCCCCCEEECCCCCCCCCCCCCEEEEECCCCHHHCCCEEEEECCCCCCCCCCCCCCCCC
ESVKVTSEAIMPISGTVRAINDALAETPELLNTAPYGEGWLIRVTALPYDAAACMNSDAY
EEEEECCCEECCCCCHHHHHHHHHHCCHHHHHCCCCCCCEEEEEEECCCCHHHHCCCCCE
AAVIGA
EEEECC
>Mature Secondary Structure
MNFPTDRIYHADHLWAQEQADGSWLIGITDYAQDQLGSVIFVDMPEQGTQFEQGQSCASI
CCCCCCCEEECCCCCCCCCCCCCEEEEECCCCHHHCCCEEEEECCCCCCCCCCCCCCCCC
ESVKVTSEAIMPISGTVRAINDALAETPELLNTAPYGEGWLIRVTALPYDAAACMNSDAY
EEEEECCCEECCCCCHHHHHHHHHHCCHHHHHCCCCCCCEEEEEEECCCCHHHHCCCCCE
AAVIGA
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]