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The map label for this gene is yciL [C]

Identifier: 220904481

GI number: 220904481

Start: 1424866

End: 1425597

Strand: Reverse

Name: yciL [C]

Synonym: Ddes_1211

Alternate gene names: 220904481

Gene position: 1425597-1424866 (Counterclockwise)

Preceding gene: 220904489

Following gene: 220904480

Centisome position: 49.61

GC content: 54.51

Gene sequence:

>732_bases
ATGCGTTTGAACAAGGCCATTGCGGCATCGGGGCTATGCTCACGCAGAAAGGCGGACGAGCTGATTCTTTCTGGTCGTGT
AACCGTCAACGGCATAGCTGAACCCAATCCCGCCCGTCAGGTCTCTCCGGAAGACGCCCTCGGCGTTGACGGCCGTCCGC
TTACCGGAGCGCAGGACTTTTACTACCTCATGCTCAACAAGCCTGTTCAGGTGGTGTGCACTGTAAATGACCCTGAGGGG
CGGCCTACGGTTATGGAGTACCTGCCGAAAAACATGCGGGCGCTACGCCTCTACCCTGTCGGCCGTCTTGATTATTTTTC
TGAAGGGTTACTATTGTTGACCAATGACGGCCAACTTGCTCAACGACTCACCCACCCCAGCCATCATCAGCCAAAAACAT
ATGAAGTTTTGGTGCGTGGGGCTGTTTCTGACGCATCACTTGCCGCCATGCGGCATGGAATGCGTCTGGCTGAAGGCGAG
GATCTTATGCCGGTGGAGGTTGAAGCTAGTTTTACCAAGGGGCGTACCCTGTTGCGCATGGTTTTGCATCAAGGGTTGAA
CCGCCAGATACGTCGTATGTGTCGCGATCTTGGCCTCACCATTCTTTATTTGAAACGCGTGTCACAAGGCCCGATTGAGC
TGGGCGGCCTTGCAAGTGGTCGTGCACGGCAGCTGACAGCCGCTGAAATCAAGGTACTGAAAGAAAGTGTCTGCTTACAG
GAGGTACATTGA

Upstream 100 bases:

>100_bases
ACGCGTGTCCAGAACCGCAGGTTCTGGAGCGAACCTGAAAAATCCAGGCTCTTGTGTGCCCCATTCGCCACAGCCTGCGG
CATTGTCAGATCCAGACCGC

Downstream 100 bases:

>100_bases
TGAAAAAACATTTTTCTACTTTACTGTTGGTTGCGGCCATATTGACGGCTAACCTGGCCACGGGGGCTATTGCCTCTGGC
AAGAAAATTGATGAACCCAT

Product: pseudouridine synthase

Products: NA

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MRLNKAIAASGLCSRRKADELILSGRVTVNGIAEPNPARQVSPEDALGVDGRPLTGAQDFYYLMLNKPVQVVCTVNDPEG
RPTVMEYLPKNMRALRLYPVGRLDYFSEGLLLLTNDGQLAQRLTHPSHHQPKTYEVLVRGAVSDASLAAMRHGMRLAEGE
DLMPVEVEASFTKGRTLLRMVLHQGLNRQIRRMCRDLGLTILYLKRVSQGPIELGGLASGRARQLTAAEIKVLKESVCLQ
EVH

Sequences:

>Translated_243_residues
MRLNKAIAASGLCSRRKADELILSGRVTVNGIAEPNPARQVSPEDALGVDGRPLTGAQDFYYLMLNKPVQVVCTVNDPEG
RPTVMEYLPKNMRALRLYPVGRLDYFSEGLLLLTNDGQLAQRLTHPSHHQPKTYEVLVRGAVSDASLAAMRHGMRLAEGE
DLMPVEVEASFTKGRTLLRMVLHQGLNRQIRRMCRDLGLTILYLKRVSQGPIELGGLASGRARQLTAAEIKVLKESVCLQ
EVH
>Mature_243_residues
MRLNKAIAASGLCSRRKADELILSGRVTVNGIAEPNPARQVSPEDALGVDGRPLTGAQDFYYLMLNKPVQVVCTVNDPEG
RPTVMEYLPKNMRALRLYPVGRLDYFSEGLLLLTNDGQLAQRLTHPSHHQPKTYEVLVRGAVSDASLAAMRHGMRLAEGE
DLMPVEVEASFTKGRTLLRMVLHQGLNRQIRRMCRDLGLTILYLKRVSQGPIELGGLASGRARQLTAAEIKVLKESVCLQ
EVH

Specific function: Unknown

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=247, Percent_Identity=37.6518218623482, Blast_Score=130, Evalue=9e-32,
Organism=Escherichia coli, GI1790453, Length=233, Percent_Identity=36.0515021459227, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1788510, Length=231, Percent_Identity=37.6623376623377, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI87081838, Length=181, Percent_Identity=34.8066298342541, Blast_Score=98, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 26892; Mature: 26892

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLNKAIAASGLCSRRKADELILSGRVTVNGIAEPNPARQVSPEDALGVDGRPLTGAQDF
CCCCHHHHHHHHHHHHCHHHEEEECCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCE
YYLMLNKPVQVVCTVNDPEGRPTVMEYLPKNMRALRLYPVGRLDYFSEGLLLLTNDGQLA
EEEEECCCEEEEEEEECCCCCHHHHHHCCCCCEEEEEEECCCCHHCCCCEEEEECCCHHH
QRLTHPSHHQPKTYEVLVRGAVSDASLAAMRHGMRLAEGEDLMPVEVEASFTKGRTLLRM
HHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCEECCCCCCCEEEEECCCHHHHHHHHH
VLHQGLNRQIRRMCRDLGLTILYLKRVSQGPIELGGLASGRARQLTAAEIKVLKESVCLQ
HHHHCCCHHHHHHHHHHCCCEEEEHHHCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHH
EVH
HCC
>Mature Secondary Structure
MRLNKAIAASGLCSRRKADELILSGRVTVNGIAEPNPARQVSPEDALGVDGRPLTGAQDF
CCCCHHHHHHHHHHHHCHHHEEEECCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCE
YYLMLNKPVQVVCTVNDPEGRPTVMEYLPKNMRALRLYPVGRLDYFSEGLLLLTNDGQLA
EEEEECCCEEEEEEEECCCCCHHHHHHCCCCCEEEEEEECCCCHHCCCCEEEEECCCHHH
QRLTHPSHHQPKTYEVLVRGAVSDASLAAMRHGMRLAEGEDLMPVEVEASFTKGRTLLRM
HHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCEECCCCCCCEEEEECCCHHHHHHHHH
VLHQGLNRQIRRMCRDLGLTILYLKRVSQGPIELGGLASGRARQLTAAEIKVLKESVCLQ
HHHHCCCHHHHHHHHHHCCCEEEEHHHCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHH
EVH
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]