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The map label for this gene is gdhA [H]

Identifier: 220904422

GI number: 220904422

Start: 1357640

End: 1358989

Strand: Reverse

Name: gdhA [H]

Synonym: Ddes_1152

Alternate gene names: 220904422

Gene position: 1358989-1357640 (Counterclockwise)

Preceding gene: 220904429

Following gene: 220904414

Centisome position: 47.29

GC content: 53.63

Gene sequence:

>1350_bases
ATGTCTTATGTACAGCGTGTAGTGAGCAACCTTCAAGATAAATACGCCTACGAACCTATCTTCCTGCAAGCTGTACAAGA
GGTGCTACAAAGCCTACAGCCCGTGCTGGATAAAAACCCCCAGTACGAACGCTACAAAATACTTGAGCGCATTGTAGAGC
CTGAGCGTACCATATCATTTCGCGTTCAATGGATTGACGATAAAGGGCAGATTCAGGTTAACAAAGGTTTTCGCGTTCAG
TATAACTCCGCCATTGGTCCCTACAAGGGCGGGCTGCGCTTTCACCCGAGTGTTAACCAGGGTATTCTGAAATTTCTTGG
CTTTGAGCAGATATTCAAAAACTCCCTCACAGGTCTGGCCATAGGCGGCGGCAAAGGCGGCGCGGATTTTGACCCCAAGG
GCAAATCCGAAACGGAAGTGATGCGCTTCTGCCAGGCATTTATGAGCGAGCTTTACCGCCACATCGGAGCCACCATTGAC
GTTCCCGCCGGTGATATCGGAGTTGGCGGACGCGAGGTAGGCTATCTTTTCGGCCAGTACAAACGCCTTACCCAGAGCTA
CGAAGGGGTGTTGACAGGAAAAAACCTTCTGTTCGGCGGCTCCCTGGCCCGCACGGAAGCCACCGGCTATGGTGCTGTAT
ACTTTGCCCAGAGCATGCTTGAGGGTAAGGGGCAAAGCCTCGAAGGTAAGAAGTGCGCTGTGTCTGGCGCAGGCAACGTA
GCTATCTACTGCTGTGAAAAACTTTATCAGGTAGGGGCCACCCCGCTTACTGTTTCTGATTCACGCGGCATGATCTATGA
CCCTGAAGGCATCAACATGGATGTGCTCAAACAAATCAAAGAAGTTGAGCGCGAATCCCTGACCCGCTACGTAGAACTGG
TTTCTTCCGCGGTGTACACTCCGGTTAATGAATACCCTGACGGCCGCAATGCCGTGTGGTCTGTTCCCTGCTATGCCGCA
TTTCCCTGCGCGACCCAGAATGAGCTGAACCTTGAAGACGCCAGAGTTCTGCTTGCCAACGGCTGCCAGTGCGTTGCCGA
AGGGGCCAATATGCCCTCTACCCTTGATGCGGTGCATGCCTTCCTTAAAGCCGGCATCTGCTATGGCCCCGCCAAGGCCG
CCAATGCCGGCGGCGTTGCCACCAGCCAGCTTGAAATGGCCCAGAACGCCAGCATGCAAAGCTGGTCTTTTGAAGCGGTG
GACAACAAGCTTCACGAGATCATGCGTAATATTTATATGAGCGCCGCTGAAACGGCCAAAGAATTCGGAGAACCCGGCAA
CCTCGTGATGGGTGCCAATATCGCCGGCTTCCGCAAGGTTGCCAACGCCATGGTGGCCCAGGGAGTATAA

Upstream 100 bases:

>100_bases
CCTTGTCAGACGCCGCTATTTTGGATTACTTAGTAATCCGCCCCAAAAACAGGAATGCCTTTTACAGCGCTTCCTGATCA
TCGCCACAAGGAGAGCGACA

Downstream 100 bases:

>100_bases
CGGCAAAGTCTTCTGACCTGCCTTCTGCAAGCCGGAGACCCAGCAGGGTTTCCGGCTTTTTTGCGCTTATGCCCTCTCTT
GCCGGCATGCTTGCCCGAAA

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: NADP-GDH [H]

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MSYVQRVVSNLQDKYAYEPIFLQAVQEVLQSLQPVLDKNPQYERYKILERIVEPERTISFRVQWIDDKGQIQVNKGFRVQ
YNSAIGPYKGGLRFHPSVNQGILKFLGFEQIFKNSLTGLAIGGGKGGADFDPKGKSETEVMRFCQAFMSELYRHIGATID
VPAGDIGVGGREVGYLFGQYKRLTQSYEGVLTGKNLLFGGSLARTEATGYGAVYFAQSMLEGKGQSLEGKKCAVSGAGNV
AIYCCEKLYQVGATPLTVSDSRGMIYDPEGINMDVLKQIKEVERESLTRYVELVSSAVYTPVNEYPDGRNAVWSVPCYAA
FPCATQNELNLEDARVLLANGCQCVAEGANMPSTLDAVHAFLKAGICYGPAKAANAGGVATSQLEMAQNASMQSWSFEAV
DNKLHEIMRNIYMSAAETAKEFGEPGNLVMGANIAGFRKVANAMVAQGV

Sequences:

>Translated_449_residues
MSYVQRVVSNLQDKYAYEPIFLQAVQEVLQSLQPVLDKNPQYERYKILERIVEPERTISFRVQWIDDKGQIQVNKGFRVQ
YNSAIGPYKGGLRFHPSVNQGILKFLGFEQIFKNSLTGLAIGGGKGGADFDPKGKSETEVMRFCQAFMSELYRHIGATID
VPAGDIGVGGREVGYLFGQYKRLTQSYEGVLTGKNLLFGGSLARTEATGYGAVYFAQSMLEGKGQSLEGKKCAVSGAGNV
AIYCCEKLYQVGATPLTVSDSRGMIYDPEGINMDVLKQIKEVERESLTRYVELVSSAVYTPVNEYPDGRNAVWSVPCYAA
FPCATQNELNLEDARVLLANGCQCVAEGANMPSTLDAVHAFLKAGICYGPAKAANAGGVATSQLEMAQNASMQSWSFEAV
DNKLHEIMRNIYMSAAETAKEFGEPGNLVMGANIAGFRKVANAMVAQGV
>Mature_448_residues
SYVQRVVSNLQDKYAYEPIFLQAVQEVLQSLQPVLDKNPQYERYKILERIVEPERTISFRVQWIDDKGQIQVNKGFRVQY
NSAIGPYKGGLRFHPSVNQGILKFLGFEQIFKNSLTGLAIGGGKGGADFDPKGKSETEVMRFCQAFMSELYRHIGATIDV
PAGDIGVGGREVGYLFGQYKRLTQSYEGVLTGKNLLFGGSLARTEATGYGAVYFAQSMLEGKGQSLEGKKCAVSGAGNVA
IYCCEKLYQVGATPLTVSDSRGMIYDPEGINMDVLKQIKEVERESLTRYVELVSSAVYTPVNEYPDGRNAVWSVPCYAAF
PCATQNELNLEDARVLLANGCQCVAEGANMPSTLDAVHAFLKAGICYGPAKAANAGGVATSQLEMAQNASMQSWSFEAVD
NKLHEIMRNIYMSAAETAKEFGEPGNLVMGANIAGFRKVANAMVAQGV

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=385, Percent_Identity=27.2727272727273, Blast_Score=135, Evalue=8e-32,
Organism=Homo sapiens, GI4885281, Length=424, Percent_Identity=26.8867924528302, Blast_Score=132, Evalue=9e-31,
Organism=Escherichia coli, GI1788059, Length=446, Percent_Identity=54.932735426009, Blast_Score=492, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI17544676, Length=358, Percent_Identity=25.9776536312849, Blast_Score=117, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6324951, Length=452, Percent_Identity=50.2212389380531, Blast_Score=390, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6319256, Length=454, Percent_Identity=48.6784140969163, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI45553475, Length=388, Percent_Identity=27.5773195876289, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI45549226, Length=388, Percent_Identity=27.5773195876289, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24649283, Length=367, Percent_Identity=26.9754768392371, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24649460, Length=299, Percent_Identity=29.4314381270903, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.4 [H]

Molecular weight: Translated: 48945; Mature: 48814

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYVQRVVSNLQDKYAYEPIFLQAVQEVLQSLQPVLDKNPQYERYKILERIVEPERTISF
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEE
RVQWIDDKGQIQVNKGFRVQYNSAIGPYKGGLRFHPSVNQGILKFLGFEQIFKNSLTGLA
EEEEECCCCCEEECCCEEEEECCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCEEE
IGGGKGGADFDPKGKSETEVMRFCQAFMSELYRHIGATIDVPAGDIGVGGREVGYLFGQY
EECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCHHHHHHHHHH
KRLTQSYEGVLTGKNLLFGGSLARTEATGYGAVYFAQSMLEGKGQSLEGKKCAVSGAGNV
HHHHHHHCCEEECCCEEECCCHHHCCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCC
AIYCCEKLYQVGATPLTVSDSRGMIYDPEGINMDVLKQIKEVERESLTRYVELVSSAVYT
HHHHHHHHHHCCCCCEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PVNEYPDGRNAVWSVPCYAAFPCATQNELNLEDARVLLANGCQCVAEGANMPSTLDAVHA
CHHCCCCCCCCEEECCCCEECCCCCCCCCCHHHHHHHHHCCCHHHHCCCCCCHHHHHHHH
FLKAGICYGPAKAANAGGVATSQLEMAQNASMQSWSFEAVDNKLHEIMRNIYMSAAETAK
HHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EFGEPGNLVMGANIAGFRKVANAMVAQGV
HHCCCCCEEEECCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SYVQRVVSNLQDKYAYEPIFLQAVQEVLQSLQPVLDKNPQYERYKILERIVEPERTISF
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEE
RVQWIDDKGQIQVNKGFRVQYNSAIGPYKGGLRFHPSVNQGILKFLGFEQIFKNSLTGLA
EEEEECCCCCEEECCCEEEEECCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCEEE
IGGGKGGADFDPKGKSETEVMRFCQAFMSELYRHIGATIDVPAGDIGVGGREVGYLFGQY
EECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCHHHHHHHHHH
KRLTQSYEGVLTGKNLLFGGSLARTEATGYGAVYFAQSMLEGKGQSLEGKKCAVSGAGNV
HHHHHHHCCEEECCCEEECCCHHHCCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCC
AIYCCEKLYQVGATPLTVSDSRGMIYDPEGINMDVLKQIKEVERESLTRYVELVSSAVYT
HHHHHHHHHHCCCCCEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PVNEYPDGRNAVWSVPCYAAFPCATQNELNLEDARVLLANGCQCVAEGANMPSTLDAVHA
CHHCCCCCCCCEEECCCCEECCCCCCCCCCHHHHHHHHHCCCHHHHCCCCCCHHHHHHHH
FLKAGICYGPAKAANAGGVATSQLEMAQNASMQSWSFEAVDNKLHEIMRNIYMSAAETAK
HHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EFGEPGNLVMGANIAGFRKVANAMVAQGV
HHCCCCCEEEECCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]