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The map label for this gene is yfjP [H]

Identifier: 220904429

GI number: 220904429

Start: 1367999

End: 1368706

Strand: Reverse

Name: yfjP [H]

Synonym: Ddes_1159

Alternate gene names: 220904429

Gene position: 1368706-1367999 (Counterclockwise)

Preceding gene: 220904430

Following gene: 220904422

Centisome position: 47.63

GC content: 52.26

Gene sequence:

>708_bases
ATGCCCAATACACATTATTTTGAATATGGCGAAAAAGAAAAATCATGGCTTAAGTCGCGCGATCCGGTGCTGGCCGCCGC
TATGGAAGAAATCGGACACATCCGGCGCGAAGTTACGCCGGACATTTTCAACGCGCTTTTAAATTCCATCGTAGGGCAAC
AGATATCAACAAAGGCACAGGCCACCATCTGGAAGCGCATGCGGGAGCAGTTCTGCCCCATAACACCGGAAAACATAGGA
ACAATAAGCGCGGAAAGCCTGCAAACCTGCGGCATATCCATGCGTAAGGCGGCCTATATCAAGAGTATTACCGAAGCTGT
GCTGGATGGAAGCCTGGATCTTGCCCGCCTGCCCTCCCTGACTGACAAGGAGATTTGCGCTCAACTGGTGCAGTTGAAAG
GCATAGGTGTATGGACTGCGGAAATGATCATGATTTTTTCCATGCAGCGGCCGGATATCCTGAGCTGGGATGATCTGGCA
ATTCAGCGTGGCCTGCGCATGCTGTACAGGCATCGCCAGATTACTCCCGCACTCTTTGCCAGATACAGAAAGCGCTATTC
CCCCCATGCGACCACAGCCAGCCTGTATTTGTGGGCTATAGCCGGGGGAGCGTGCGCCGAACTTAAAGACTGCGCGCCGC
TAAAAAAGAAAACCAAGCCTGCAAAGCGGCAACCGGTCAAAGCACAGGACAAACAGAACACACAGTAA

Upstream 100 bases:

>100_bases
GTCATCGGGCATGACGGCAGCCTTACCGGATATGCGGGTGGCCTTGCCATCAAGCAATACCTGCTTGACCATGAAAAAAT
GTGGCAACCAGAGAGAATCC

Downstream 100 bases:

>100_bases
CCTTGTTTTTTCCGCATGGTGACGTGACTGGCGCGCATACATCCCTTGATGCACCGCAATGCTTCAGCCCTGCCCGAATA
TTGAGCGTGCCCACTCCTGA

Product: HhH-GPD family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 235; Mature: 234

Protein sequence:

>235_residues
MPNTHYFEYGEKEKSWLKSRDPVLAAAMEEIGHIRREVTPDIFNALLNSIVGQQISTKAQATIWKRMREQFCPITPENIG
TISAESLQTCGISMRKAAYIKSITEAVLDGSLDLARLPSLTDKEICAQLVQLKGIGVWTAEMIMIFSMQRPDILSWDDLA
IQRGLRMLYRHRQITPALFARYRKRYSPHATTASLYLWAIAGGACAELKDCAPLKKKTKPAKRQPVKAQDKQNTQ

Sequences:

>Translated_235_residues
MPNTHYFEYGEKEKSWLKSRDPVLAAAMEEIGHIRREVTPDIFNALLNSIVGQQISTKAQATIWKRMREQFCPITPENIG
TISAESLQTCGISMRKAAYIKSITEAVLDGSLDLARLPSLTDKEICAQLVQLKGIGVWTAEMIMIFSMQRPDILSWDDLA
IQRGLRMLYRHRQITPALFARYRKRYSPHATTASLYLWAIAGGACAELKDCAPLKKKTKPAKRQPVKAQDKQNTQ
>Mature_234_residues
PNTHYFEYGEKEKSWLKSRDPVLAAAMEEIGHIRREVTPDIFNALLNSIVGQQISTKAQATIWKRMREQFCPITPENIGT
ISAESLQTCGISMRKAAYIKSITEAVLDGSLDLARLPSLTDKEICAQLVQLKGIGVWTAEMIMIFSMQRPDILSWDDLAI
QRGLRMLYRHRQITPALFARYRKRYSPHATTASLYLWAIAGGACAELKDCAPLKKKTKPAKRQPVKAQDKQNTQ

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, 3-methylguanine, 7-methylguanine, O2- methylthymine, and O2-methylcytosine from the damaged DNA polymer formed by alkylation lesions [H]

COG id: COG0122

COG function: function code L; 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkylbase DNA glycosidase alkA family [H]

Homologues:

Organism=Escherichia coli, GI1788383, Length=174, Percent_Identity=26.4367816091954, Blast_Score=60, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR003265
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00730 HhH-GPD [H]

EC number: =3.2.2.21 [H]

Molecular weight: Translated: 26580; Mature: 26449

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNTHYFEYGEKEKSWLKSRDPVLAAAMEEIGHIRREVTPDIFNALLNSIVGQQISTKAQ
CCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
ATIWKRMREQFCPITPENIGTISAESLQTCGISMRKAAYIKSITEAVLDGSLDLARLPSL
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHCCCC
TDKEICAQLVQLKGIGVWTAEMIMIFSMQRPDILSWDDLAIQRGLRMLYRHRQITPALFA
CCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHH
RYRKRYSPHATTASLYLWAIAGGACAELKDCAPLKKKTKPAKRQPVKAQDKQNTQ
HHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PNTHYFEYGEKEKSWLKSRDPVLAAAMEEIGHIRREVTPDIFNALLNSIVGQQISTKAQ
CCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
ATIWKRMREQFCPITPENIGTISAESLQTCGISMRKAAYIKSITEAVLDGSLDLARLPSL
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHCCCC
TDKEICAQLVQLKGIGVWTAEMIMIFSMQRPDILSWDDLAIQRGLRMLYRHRQITPALFA
CCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHH
RYRKRYSPHATTASLYLWAIAGGACAELKDCAPLKKKTKPAKRQPVKAQDKQNTQ
HHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]