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The map label for this gene is rpoD [H]

Identifier: 220904357

GI number: 220904357

Start: 1270700

End: 1272469

Strand: Reverse

Name: rpoD [H]

Synonym: Ddes_1085

Alternate gene names: 220904357

Gene position: 1272469-1270700 (Counterclockwise)

Preceding gene: 220904358

Following gene: 220904356

Centisome position: 44.28

GC content: 51.3

Gene sequence:

>1770_bases
ATGAGCAGTCTTAAAGATATCCAGCAGATTCAATCACTTATTGCCAAAGGCAAGGGTGCGGGCTTTTTGACCTTTGAAGA
GGTCAACAAAGCGCTGCCCGTTGAAATGAGCACGCCCGAGCAGTTTGAAGAGATCATAGGCATCTTTGAACAGCTGGAAA
TCGTTATTGTGGACTCAGAGAAGGACGGAAAAAAAATTTCGGCCACTGCCTCTGAATCTGACGAGGAAATAACCGACAGT
TCCCTTGATCTGTCTGAGGACGAAGATACTGCGGACTATTCTTCCCGCAGCACAGACCCCGTGCGCATGTACTTGCGCGA
AATGGGGGCTGTTCCCCTGCTTGACCGCGATGGTGAAGTTGTTATCGCCAAAAAGATTGAAATGGGCGAACAAGACGTGC
TTTATGCCCTTGTTGAAGTTCCTGTGGCAGTTGAAGAGCTGATCAATGTGGGCGAAGACCTGCGCCAGAACCGCATCAAG
CTCAAGGATGTGGTAAAAACCATTGAAGAAGACGACCCCAGCGAAGATGAAATGAACCAGCGTTCGCGCGTCATCCTGCT
GCTGGACGAAATCAAGCAGACCTTCAAGAAAAAACGCAAGATCTATAAAAAACTTGATGTCTGCACCTGCATGGATCGCC
GCGTCACCGCCGTGCAGAAAGAAATCATCTGCTTCAAGGAAGAAATTGTTACCCGCCTGCGTGATATCAAGCTGGAAAAA
ACCCTCATTGACCGCATTATTGAAACGGTTGAGGACTACGTGCGCCAGATGCACAACTGCCAGCGTGACCTTTCTGCCTA
CATTCTTGCCACCGGCCGTACCCAGGCGGAAATTCAGGCCATGTTTGACGGCCTTGAGCAGCGCGAAATCAATCCGATGG
ATGCCGCCCGCGACCTCAAGCTCAGTGTGGACGAACTCTTTTCCTTCAAGGAAATGATCATCGGCAAGATTGAAATCCTC
AACCGCCTGCAGGAAAAATGCTGCCATAACGTCACGGACCTTGAAGAGGTCCTGTGGCGCATCAAACGCGGCAATACGGC
GGCCATGCGCGCCAAGCAGGAACTTATCCGCTCCAACCTGCGCCTTGTGGTTTCCATTGCAAAAAAATATACCAATCGTG
GTCTCCAGTTCCTCGACCTTATTCAGGAAGGCAATATCGGCCTCATGAAAGCTGTGGATAAGTTTGAGTATCAGCGCGGC
TATAAATTCTCTACGTATGCCACATGGTGGATCCGGCAGGCCATTACCCGCGCCATTGCCGACCAGGCGCGCACCATCCG
TATCCCCGTGCATATGATTGAAACCATCAACAAGCTCATACGCACATCGCGCTACCTTGTGCAGGAGCTGGGCCGCGACC
CCACGCCCGAAGAAATCGCCGAACGTATGGAATACCCAGTGGAAAAGGTCAAAAAGGTACTCAAAATCGCCAAAGAACCC
ATTTCTCTGGAAACTCCTATCGGCGATGAAGAAGATTCAAGCCTGGGCGATTTTATTGAAGATAAAAAAGCTGTGGCCCC
GGCAGAAGAAGTTATCAACACCAAGCTGTCGGAACAGCTGGCCGCAGTGCTGGCAGACCTCACCCCTCGTGAGGAGCAGG
TTTTGCGCAAACGCTTCGGCATTGCTGAAAAGAGTGATCACACCCTTGAAGAAGTGGGCAAGCTCTTTAACGTGACCCGC
GAACGTATCCGGCAGATCGAGGCCAAGGCTTTACGCAAACTGCGTCATCCCGTGCGCAGCCAGCCCCTGCGCTCTTATTA
CGAAAACTGA

Upstream 100 bases:

>100_bases
TCTTCTTTATCCGCAGCCTTGAGGGCCAACACCGGCACGGGTGACTTTGAGGCTGATATGGACTATCTTCGCGCACTTAA
GGAAACCTTGGAGAAAGGGC

Downstream 100 bases:

>100_bases
TTTCCCGGCAGCGGCCTGGCATGCTGCCAGGCCGCTGCCGCATGATACAGGCCAGGGCATACGCTCTGCGCGGCATACCA
AAATCCGCAAGGTTTTCCGG

Product: RpoD subfamily RNA polymerase sigma-70 subunit

Products: NA

Alternate protein names: Sigma-80 [H]

Number of amino acids: Translated: 589; Mature: 588

Protein sequence:

>589_residues
MSSLKDIQQIQSLIAKGKGAGFLTFEEVNKALPVEMSTPEQFEEIIGIFEQLEIVIVDSEKDGKKISATASESDEEITDS
SLDLSEDEDTADYSSRSTDPVRMYLREMGAVPLLDRDGEVVIAKKIEMGEQDVLYALVEVPVAVEELINVGEDLRQNRIK
LKDVVKTIEEDDPSEDEMNQRSRVILLLDEIKQTFKKKRKIYKKLDVCTCMDRRVTAVQKEIICFKEEIVTRLRDIKLEK
TLIDRIIETVEDYVRQMHNCQRDLSAYILATGRTQAEIQAMFDGLEQREINPMDAARDLKLSVDELFSFKEMIIGKIEIL
NRLQEKCCHNVTDLEEVLWRIKRGNTAAMRAKQELIRSNLRLVVSIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYQRG
YKFSTYATWWIRQAITRAIADQARTIRIPVHMIETINKLIRTSRYLVQELGRDPTPEEIAERMEYPVEKVKKVLKIAKEP
ISLETPIGDEEDSSLGDFIEDKKAVAPAEEVINTKLSEQLAAVLADLTPREEQVLRKRFGIAEKSDHTLEEVGKLFNVTR
ERIRQIEAKALRKLRHPVRSQPLRSYYEN

Sequences:

>Translated_589_residues
MSSLKDIQQIQSLIAKGKGAGFLTFEEVNKALPVEMSTPEQFEEIIGIFEQLEIVIVDSEKDGKKISATASESDEEITDS
SLDLSEDEDTADYSSRSTDPVRMYLREMGAVPLLDRDGEVVIAKKIEMGEQDVLYALVEVPVAVEELINVGEDLRQNRIK
LKDVVKTIEEDDPSEDEMNQRSRVILLLDEIKQTFKKKRKIYKKLDVCTCMDRRVTAVQKEIICFKEEIVTRLRDIKLEK
TLIDRIIETVEDYVRQMHNCQRDLSAYILATGRTQAEIQAMFDGLEQREINPMDAARDLKLSVDELFSFKEMIIGKIEIL
NRLQEKCCHNVTDLEEVLWRIKRGNTAAMRAKQELIRSNLRLVVSIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYQRG
YKFSTYATWWIRQAITRAIADQARTIRIPVHMIETINKLIRTSRYLVQELGRDPTPEEIAERMEYPVEKVKKVLKIAKEP
ISLETPIGDEEDSSLGDFIEDKKAVAPAEEVINTKLSEQLAAVLADLTPREEQVLRKRFGIAEKSDHTLEEVGKLFNVTR
ERIRQIEAKALRKLRHPVRSQPLRSYYEN
>Mature_588_residues
SSLKDIQQIQSLIAKGKGAGFLTFEEVNKALPVEMSTPEQFEEIIGIFEQLEIVIVDSEKDGKKISATASESDEEITDSS
LDLSEDEDTADYSSRSTDPVRMYLREMGAVPLLDRDGEVVIAKKIEMGEQDVLYALVEVPVAVEELINVGEDLRQNRIKL
KDVVKTIEEDDPSEDEMNQRSRVILLLDEIKQTFKKKRKIYKKLDVCTCMDRRVTAVQKEIICFKEEIVTRLRDIKLEKT
LIDRIIETVEDYVRQMHNCQRDLSAYILATGRTQAEIQAMFDGLEQREINPMDAARDLKLSVDELFSFKEMIIGKIEILN
RLQEKCCHNVTDLEEVLWRIKRGNTAAMRAKQELIRSNLRLVVSIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYQRGY
KFSTYATWWIRQAITRAIADQARTIRIPVHMIETINKLIRTSRYLVQELGRDPTPEEIAERMEYPVEKVKKVLKIAKEPI
SLETPIGDEEDSSLGDFIEDKKAVAPAEEVINTKLSEQLAAVLADLTPREEQVLRKRFGIAEKSDHTLEEVGKLFNVTRE
RIRQIEAKALRKLRHPVRSQPLRSYYEN

Specific function: Also required for extracellular A-signal production. The A-signal is the first in a series of extracellular signals that are required for myxspore differentiation in M.xanthus. It is thought to act as a cell-density signal during early development to ensu

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=286, Percent_Identity=64.3356643356643, Blast_Score=372, Evalue=1e-104,
Organism=Escherichia coli, GI1789098, Length=255, Percent_Identity=43.5294117647059, Blast_Score=225, Evalue=7e-60,
Organism=Escherichia coli, GI1789871, Length=249, Percent_Identity=28.5140562248996, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1788231, Length=229, Percent_Identity=26.2008733624454, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 67843; Mature: 67712

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLKDIQQIQSLIAKGKGAGFLTFEEVNKALPVEMSTPEQFEEIIGIFEQLEIVIVDSE
CCHHHHHHHHHHHHHCCCCCCEEEHHHHHCCCCCCCCCHHHHHHHHHHHHHCEEEEEECC
KDGKKISATASESDEEITDSSLDLSEDEDTADYSSRSTDPVRMYLREMGAVPLLDRDGEV
CCCCEEECCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEECCCCCE
VIAKKIEMGEQDVLYALVEVPVAVEELINVGEDLRQNRIKLKDVVKTIEEDDPSEDEMNQ
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHC
RSRVILLLDEIKQTFKKKRKIYKKLDVCTCMDRRVTAVQKEIICFKEEIVTRLRDIKLEK
HHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLIDRIIETVEDYVRQMHNCQRDLSAYILATGRTQAEIQAMFDGLEQREINPMDAARDLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHCCCCHHHHHHHHH
LSVDELFSFKEMIIGKIEILNRLQEKCCHNVTDLEEVLWRIKRGNTAAMRAKQELIRSNL
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHH
RLVVSIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYQRGYKFSTYATWWIRQAITRAIA
HHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
DQARTIRIPVHMIETINKLIRTSRYLVQELGRDPTPEEIAERMEYPVEKVKKVLKIAKEP
HCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHCCC
ISLETPIGDEEDSSLGDFIEDKKAVAPAEEVINTKLSEQLAAVLADLTPREEQVLRKRFG
CCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHC
IAEKSDHTLEEVGKLFNVTRERIRQIEAKALRKLRHPVRSQPLRSYYEN
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCC
>Mature Secondary Structure 
SSLKDIQQIQSLIAKGKGAGFLTFEEVNKALPVEMSTPEQFEEIIGIFEQLEIVIVDSE
CHHHHHHHHHHHHHCCCCCCEEEHHHHHCCCCCCCCCHHHHHHHHHHHHHCEEEEEECC
KDGKKISATASESDEEITDSSLDLSEDEDTADYSSRSTDPVRMYLREMGAVPLLDRDGEV
CCCCEEECCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEECCCCCE
VIAKKIEMGEQDVLYALVEVPVAVEELINVGEDLRQNRIKLKDVVKTIEEDDPSEDEMNQ
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHC
RSRVILLLDEIKQTFKKKRKIYKKLDVCTCMDRRVTAVQKEIICFKEEIVTRLRDIKLEK
HHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLIDRIIETVEDYVRQMHNCQRDLSAYILATGRTQAEIQAMFDGLEQREINPMDAARDLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHCCCCHHHHHHHHH
LSVDELFSFKEMIIGKIEILNRLQEKCCHNVTDLEEVLWRIKRGNTAAMRAKQELIRSNL
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHH
RLVVSIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYQRGYKFSTYATWWIRQAITRAIA
HHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
DQARTIRIPVHMIETINKLIRTSRYLVQELGRDPTPEEIAERMEYPVEKVKKVLKIAKEP
HCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHCCC
ISLETPIGDEEDSSLGDFIEDKKAVAPAEEVINTKLSEQLAAVLADLTPREEQVLRKRFG
CCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHC
IAEKSDHTLEEVGKLFNVTRERIRQIEAKALRKLRHPVRSQPLRSYYEN
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2104614 [H]