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The map label for this gene is dnaG [H]

Identifier: 220904358

GI number: 220904358

Start: 1272456

End: 1274198

Strand: Reverse

Name: dnaG [H]

Synonym: Ddes_1086

Alternate gene names: 220904358

Gene position: 1274198-1272456 (Counterclockwise)

Preceding gene: 220904359

Following gene: 220904357

Centisome position: 44.34

GC content: 57.77

Gene sequence:

>1743_bases
ATGCAGTCCAGGGATGCCATACGCGCCATCAAGGAGCGCATGAATATTGTGGACGTTGTGCGCCGCTATGTAGAGCTGAA
GCGCAACGGGCCGCGCTGGGTGGCGCCCTGCCCTTTTCATCAGGAAACAAAACCGTCCTTTTCCGTCAATGAGGATCAGG
GCCTGTTTTACTGCTTTGGCTGCCATGCTTCGGGCGATATCTTCGATTTTTACAGCCGTATCAACGGGCTGGATTTTAAA
GAAACGCTTGAACAACTGGCTGCCGAGGCGGGCATAACCATCGAACGCGGTCCCGGCGGTGCGCGCCGCCAAGGTGAAGA
GCGTGAGCGCCGCTCTTCACGCCAGCAGATGTTGCGCATGTACGAATTGGCTGCAGGCCATTTTGCGGCTACGCTCAACG
GCCCGGACGGTGCAGAATGTCGCACATATATGGAAAAACGCGGCCTCAGCCAGGAAATTGTGCAGCATTTTGGCCTTGGC
TGGGCCAAAAGGGAATGGCAGTCACTGGCAGACTCGCTGCGCCGTGCGGGCTTTGACGCCCGTATGGCAATCGAGGCTGG
CCTTTTGGGGCAATCCGATAATGGGCGGGCGTATGACCGCTTCCGGGGGCGGCTCATTTTTCCCATCAAGAGCCTATCCA
ACCAGATCATTGCCTTTGGCGGACGCATCATTGCCGATGAAGACGAGGCCAAGTACATTAATAGCGCCGACACGCCCATC
TATAAAAAAGGCGAGCACCTCTACGGCCTGGCGCAGGCTCGCAAGGGTATGGTCACCAAAGGGCGCGCCCTGCTGACTGA
GGGCTACATGGATGTGCTTACCCTGCACCAGTTCGGCTACGACAATGCTGTGGGGGTTCTCGGCACAGCCCTGACGCCGG
AACAGATCAAACGGCTCTCGGGTTTTGTTTCGCAACTGACCCTGCTCTTTGACGGCGACCGGGCCGGGCGCAAAGCCGCC
CTGCGTTCCTGCGAAATGCTGCTCACACGCGGCCTGGCCTGTTCGGTCGTGCTTATGCCCGAGGGCGAAGACATCGACAG
CCTGCTACGCGGTTCAGGACCGGAGGCATTTGAAGAACTGCAAGCCCAGGCCCCGGACGGGCTGCGTTTTTGCGTGGACG
TGCTCAAGGCTCTGGCCCCCAGAGAAACCGTAGAATGGGCGCGTAACTTCCTGCGTCAGGTGGAAATTCCCGAACTGGTC
AGCCCGTATGTTTCGCGGCTGGCCGGGCACCTGCAACTTTCAGAAGCCGACCTGCGTGAAGGGCTTATGCAGTCGCGCGG
GGTAAAAAACGACAAGACACGTCCTGGTTCAAACAGTTTGCCGTCGGGGCGTGTCCTGCAAAACATTCGCGACCGCGAAA
TCATGATGTTCGCCGTGCGTTACCCGCACCGGCTGACAAACATGCTTGAACTCGGGGCCGACCTGGCTCTGCAATCGGCA
GTGGCGCGTCAGTTATGGGACAAAATTGAAACCCACGGCGCGGATGAGGTATTTCACCATCTGGACCAACGGGAAAAAAA
TTTCTGGTGCAAGTGCTGCGGCCCCGAGGCCGCCCCGCGCGATAACGGCGACAGGGAACTGGAATCATTGCGCCATTACT
TGAACAATTACTACGCTTCGGCGCAAAAATCTTCTTTATCCGCAGCCTTGAGGGCCAACACCGGCACGGGTGACTTTGAG
GCTGATATGGACTATCTTCGCGCACTTAAGGAAACCTTGGAGAAAGGGCATGAGCAGTCTTAA

Upstream 100 bases:

>100_bases
GCAAGCAGTCTGCCACTTCGTCTGTACGCAGCACGAACGCCCTGCCCGGCAGGTGCATAAACAGTTTTTCCAGTAAAGCG
GAAAGGAAGATTATCAGTCA

Downstream 100 bases:

>100_bases
AGATATCCAGCAGATTCAATCACTTATTGCCAAAGGCAAGGGTGCGGGCTTTTTGACCTTTGAAGAGGTCAACAAAGCGC
TGCCCGTTGAAATGAGCACG

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 580; Mature: 580

Protein sequence:

>580_residues
MQSRDAIRAIKERMNIVDVVRRYVELKRNGPRWVAPCPFHQETKPSFSVNEDQGLFYCFGCHASGDIFDFYSRINGLDFK
ETLEQLAAEAGITIERGPGGARRQGEERERRSSRQQMLRMYELAAGHFAATLNGPDGAECRTYMEKRGLSQEIVQHFGLG
WAKREWQSLADSLRRAGFDARMAIEAGLLGQSDNGRAYDRFRGRLIFPIKSLSNQIIAFGGRIIADEDEAKYINSADTPI
YKKGEHLYGLAQARKGMVTKGRALLTEGYMDVLTLHQFGYDNAVGVLGTALTPEQIKRLSGFVSQLTLLFDGDRAGRKAA
LRSCEMLLTRGLACSVVLMPEGEDIDSLLRGSGPEAFEELQAQAPDGLRFCVDVLKALAPRETVEWARNFLRQVEIPELV
SPYVSRLAGHLQLSEADLREGLMQSRGVKNDKTRPGSNSLPSGRVLQNIRDREIMMFAVRYPHRLTNMLELGADLALQSA
VARQLWDKIETHGADEVFHHLDQREKNFWCKCCGPEAAPRDNGDRELESLRHYLNNYYASAQKSSLSAALRANTGTGDFE
ADMDYLRALKETLEKGHEQS

Sequences:

>Translated_580_residues
MQSRDAIRAIKERMNIVDVVRRYVELKRNGPRWVAPCPFHQETKPSFSVNEDQGLFYCFGCHASGDIFDFYSRINGLDFK
ETLEQLAAEAGITIERGPGGARRQGEERERRSSRQQMLRMYELAAGHFAATLNGPDGAECRTYMEKRGLSQEIVQHFGLG
WAKREWQSLADSLRRAGFDARMAIEAGLLGQSDNGRAYDRFRGRLIFPIKSLSNQIIAFGGRIIADEDEAKYINSADTPI
YKKGEHLYGLAQARKGMVTKGRALLTEGYMDVLTLHQFGYDNAVGVLGTALTPEQIKRLSGFVSQLTLLFDGDRAGRKAA
LRSCEMLLTRGLACSVVLMPEGEDIDSLLRGSGPEAFEELQAQAPDGLRFCVDVLKALAPRETVEWARNFLRQVEIPELV
SPYVSRLAGHLQLSEADLREGLMQSRGVKNDKTRPGSNSLPSGRVLQNIRDREIMMFAVRYPHRLTNMLELGADLALQSA
VARQLWDKIETHGADEVFHHLDQREKNFWCKCCGPEAAPRDNGDRELESLRHYLNNYYASAQKSSLSAALRANTGTGDFE
ADMDYLRALKETLEKGHEQS
>Mature_580_residues
MQSRDAIRAIKERMNIVDVVRRYVELKRNGPRWVAPCPFHQETKPSFSVNEDQGLFYCFGCHASGDIFDFYSRINGLDFK
ETLEQLAAEAGITIERGPGGARRQGEERERRSSRQQMLRMYELAAGHFAATLNGPDGAECRTYMEKRGLSQEIVQHFGLG
WAKREWQSLADSLRRAGFDARMAIEAGLLGQSDNGRAYDRFRGRLIFPIKSLSNQIIAFGGRIIADEDEAKYINSADTPI
YKKGEHLYGLAQARKGMVTKGRALLTEGYMDVLTLHQFGYDNAVGVLGTALTPEQIKRLSGFVSQLTLLFDGDRAGRKAA
LRSCEMLLTRGLACSVVLMPEGEDIDSLLRGSGPEAFEELQAQAPDGLRFCVDVLKALAPRETVEWARNFLRQVEIPELV
SPYVSRLAGHLQLSEADLREGLMQSRGVKNDKTRPGSNSLPSGRVLQNIRDREIMMFAVRYPHRLTNMLELGADLALQSA
VARQLWDKIETHGADEVFHHLDQREKNFWCKCCGPEAAPRDNGDRELESLRHYLNNYYASAQKSSLSAALRANTGTGDFE
ADMDYLRALKETLEKGHEQS

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=367, Percent_Identity=38.9645776566758, Blast_Score=259, Evalue=4e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 65121; Mature: 65121

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSRDAIRAIKERMNIVDVVRRYVELKRNGPRWVAPCPFHQETKPSFSVNEDQGLFYCFG
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCCEEEEEE
CHASGDIFDFYSRINGLDFKETLEQLAAEAGITIERGPGGARRQGEERERRSSRQQMLRM
CCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHH
YELAAGHFAATLNGPDGAECRTYMEKRGLSQEIVQHFGLGWAKREWQSLADSLRRAGFDA
HHHHHCCEEEECCCCCCHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCH
RMAIEAGLLGQSDNGRAYDRFRGRLIFPIKSLSNQIIAFGGRIIADEDEAKYINSADTPI
HHHHHHCCCCCCCCCCHHHHHCCEEEEEHHHHCCHHHHCCCEEEECCHHHHHCCCCCCCC
YKKGEHLYGLAQARKGMVTKGRALLTEGYMDVLTLHQFGYDNAVGVLGTALTPEQIKRLS
CCCCCHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHH
GFVSQLTLLFDGDRAGRKAALRSCEMLLTRGLACSVVLMPEGEDIDSLLRGSGPEAFEEL
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHCCCCHHHHHHH
QAQAPDGLRFCVDVLKALAPRETVEWARNFLRQVEIPELVSPYVSRLAGHLQLSEADLRE
HHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHH
GLMQSRGVKNDKTRPGSNSLPSGRVLQNIRDREIMMFAVRYPHRLTNMLELGADLALQSA
HHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCCCEEEHHHHCCHHHHHHHHHHHHHHHHHH
VARQLWDKIETHGADEVFHHLDQREKNFWCKCCGPEAAPRDNGDRELESLRHYLNNYYAS
HHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHH
AQKSSLSAALRANTGTGDFEADMDYLRALKETLEKGHEQS
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MQSRDAIRAIKERMNIVDVVRRYVELKRNGPRWVAPCPFHQETKPSFSVNEDQGLFYCFG
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCCEEEEEE
CHASGDIFDFYSRINGLDFKETLEQLAAEAGITIERGPGGARRQGEERERRSSRQQMLRM
CCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHH
YELAAGHFAATLNGPDGAECRTYMEKRGLSQEIVQHFGLGWAKREWQSLADSLRRAGFDA
HHHHHCCEEEECCCCCCHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCH
RMAIEAGLLGQSDNGRAYDRFRGRLIFPIKSLSNQIIAFGGRIIADEDEAKYINSADTPI
HHHHHHCCCCCCCCCCHHHHHCCEEEEEHHHHCCHHHHCCCEEEECCHHHHHCCCCCCCC
YKKGEHLYGLAQARKGMVTKGRALLTEGYMDVLTLHQFGYDNAVGVLGTALTPEQIKRLS
CCCCCHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHH
GFVSQLTLLFDGDRAGRKAALRSCEMLLTRGLACSVVLMPEGEDIDSLLRGSGPEAFEEL
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHCCCCHHHHHHH
QAQAPDGLRFCVDVLKALAPRETVEWARNFLRQVEIPELVSPYVSRLAGHLQLSEADLRE
HHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHH
GLMQSRGVKNDKTRPGSNSLPSGRVLQNIRDREIMMFAVRYPHRLTNMLELGADLALQSA
HHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCCCEEEHHHHCCHHHHHHHHHHHHHHHHHH
VARQLWDKIETHGADEVFHHLDQREKNFWCKCCGPEAAPRDNGDRELESLRHYLNNYYAS
HHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHH
AQKSSLSAALRANTGTGDFEADMDYLRALKETLEKGHEQS
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]