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The map label for this gene is gph [C]

Identifier: 220904352

GI number: 220904352

Start: 1265913

End: 1266569

Strand: Reverse

Name: gph [C]

Synonym: Ddes_1080

Alternate gene names: 220904352

Gene position: 1266569-1265913 (Counterclockwise)

Preceding gene: 220904354

Following gene: 220904351

Centisome position: 44.08

GC content: 60.73

Gene sequence:

>657_bases
ATGAGAGTCTTTTTCTTTGACCTGGACGGTACCCTGCTTGATACGCTTGGCGATATCGGCAATGCCTGCAATGCCGTACT
GGCCCGGCACGGTTACCCCACGCACCCTCTGGCGGACTACAGACGCTTTGTGGGACGGGGCTTTGACAAGCTGGTACGCG
ACACCCTGCCTGCCGCAGCAGAGCTTGAGCCGCCGGCCCTGACACAACTGGTGGAAGAAACCCGCCAGCACTACGGCCGC
CATATGTGCGACACCACCCGCCCCTATGAAGGCATCATCCCGGCACTGGAAACCCTGGCCGCCAAGGGATGCCCGCTGGC
CGTGCTTTCCAACAAGCCGGAAGAACATACGGTGGATCTGGTGCAGCGCTACTTTCCTTCCATCCCCTTCGTTCTTGTAC
GCGGCGGCAGAAAAAATGTTCCTCTTAAACCACAGCCGCAGGCCCTGCTGGACATGGCTGAAACAATGCATACTTCTGTG
GCCCGGGTCCTGTACGTGGGCGACAGCGACGTGGATGTGCAGACCGCGCGCAACGCGGGCACAACGTCAGTGGGTGTGGC
CTGGGGTTTTCGCGGCCCTGCGGAACTGCGCGCCGCTGGAGCCGATCATATCATCGACGCCCCGGCCCAACTGATTGAAC
TGACGGTGGAGATATAG

Upstream 100 bases:

>100_bases
TTAAAGCCCACTGTTTTTTTGACCGCGATGCGGGCTTGTGATTCATAGTGCTTTTCCGCTACACTGTTTTTACTGCTGCA
AAGAATACATGGGAGATTTG

Downstream 100 bases:

>100_bases
CCATGGCAAAACGTCTTATTATCGAAATTGACGAAGACAAGTGCAACGGCTGCGGCCAATGTGTACTGGACTGTGCCGAA
GGCGCGCTGGCCATAGTGGA

Product: HAD-superfamily hydrolase

Products: NA

Alternate protein names: PGP; PGPase [H]

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MRVFFFDLDGTLLDTLGDIGNACNAVLARHGYPTHPLADYRRFVGRGFDKLVRDTLPAAAELEPPALTQLVEETRQHYGR
HMCDTTRPYEGIIPALETLAAKGCPLAVLSNKPEEHTVDLVQRYFPSIPFVLVRGGRKNVPLKPQPQALLDMAETMHTSV
ARVLYVGDSDVDVQTARNAGTTSVGVAWGFRGPAELRAAGADHIIDAPAQLIELTVEI

Sequences:

>Translated_218_residues
MRVFFFDLDGTLLDTLGDIGNACNAVLARHGYPTHPLADYRRFVGRGFDKLVRDTLPAAAELEPPALTQLVEETRQHYGR
HMCDTTRPYEGIIPALETLAAKGCPLAVLSNKPEEHTVDLVQRYFPSIPFVLVRGGRKNVPLKPQPQALLDMAETMHTSV
ARVLYVGDSDVDVQTARNAGTTSVGVAWGFRGPAELRAAGADHIIDAPAQLIELTVEI
>Mature_218_residues
MRVFFFDLDGTLLDTLGDIGNACNAVLARHGYPTHPLADYRRFVGRGFDKLVRDTLPAAAELEPPALTQLVEETRQHYGR
HMCDTTRPYEGIIPALETLAAKGCPLAVLSNKPEEHTVDLVQRYFPSIPFVLVRGGRKNVPLKPQPQALLDMAETMHTSV
ARVLYVGDSDVDVQTARNAGTTSVGVAWGFRGPAELRAAGADHIIDAPAQLIELTVEI

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=209, Percent_Identity=29.1866028708134, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833
- InterPro:   IPR000150
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 23826; Mature: 23826

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVFFFDLDGTLLDTLGDIGNACNAVLARHGYPTHPLADYRRFVGRGFDKLVRDTLPAAA
CEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHCCCCC
ELEPPALTQLVEETRQHYGRHMCDTTRPYEGIIPALETLAAKGCPLAVLSNKPEEHTVDL
CCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHH
VQRYFPSIPFVLVRGGRKNVPLKPQPQALLDMAETMHTSVARVLYVGDSDVDVQTARNAG
HHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHCCCC
TTSVGVAWGFRGPAELRAAGADHIIDAPAQLIELTVEI
CCEEEEEECCCCHHHHHHCCCCHHHHCHHHHHHHHCCC
>Mature Secondary Structure
MRVFFFDLDGTLLDTLGDIGNACNAVLARHGYPTHPLADYRRFVGRGFDKLVRDTLPAAA
CEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHCCCCC
ELEPPALTQLVEETRQHYGRHMCDTTRPYEGIIPALETLAAKGCPLAVLSNKPEEHTVDL
CCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHH
VQRYFPSIPFVLVRGGRKNVPLKPQPQALLDMAETMHTSVARVLYVGDSDVDVQTARNAG
HHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHCCCC
TTSVGVAWGFRGPAELRAAGADHIIDAPAQLIELTVEI
CCEEEEEECCCCHHHHHHCCCCHHHHCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA