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The map label for this gene is metA

Identifier: 220904350

GI number: 220904350

Start: 1264052

End: 1264996

Strand: Reverse

Name: metA

Synonym: Ddes_1078

Alternate gene names: 220904350

Gene position: 1264996-1264052 (Counterclockwise)

Preceding gene: 220904351

Following gene: 220904349

Centisome position: 44.02

GC content: 55.24

Gene sequence:

>945_bases
ATGCCCATCAAGATCCCGGCGGATTTGCCCGCCTGCGCCGCCCTGGAAAGTGAAAACATCTTCGTAATGACCGAAGACCG
CGCAGTCGCTCAGGACATACGCCCTCTGGAAATAGTCATCGTCAACCTCATGCCCACTAAAATCGCCACTGAAACCCAGT
TATTGCGACTTTTGGGCAACTCCCCTCTTCAGGTCAACATCACCCTGCTGCGCACCGAGGCGCATGAGTCCAAAAACACA
CCGGCTCAGCATCTTGAACGTTTTTACAAAACATTCAACGAGATACGGCACGGCAGCTTTGACGGCATGATTGTCACCGG
AGCGCCGGTGGAACATCTGCCCTTTGAGGATGTGGACTACTGGCACGAACTGCTCGACATCATGAATTATGCGGCGAGCC
ATGTTTACTCCACCCTGTATATCTGCTGGGCGGCCCAGGCTGCGTTGTACCATTTTTACGGCATTCCCAAATTCAGCCTG
CCGCAAAAAATCTCAGGGATTTTCAACCACGACATTCTGCTGCCGGACTGCCGCCTTTTCCGCGGCTTTGACGACACATT
CACCGCGCCGCACTCCCGCAATACCGAAGTGCGCGCAGGGCACATCCTTGAAACGCCACAACTGCGTCTGCTTGCACAGT
CGGAACAGGCCGGAGTAACGCTTGTAGAACGCGTTGACCATTCACAGGTTTTCATGACCGGCCATCTGGAGTACGACAGA
GGCACACTGGACGCGGAATATCGCCGTGATGTGGACAGGGGCCTGAAACCGCGTGTACCGGAACATTATTATCCCCATGA
CGACCCCGCCGCTATGCCGCGCATGAACTGGCGGGCACACGCGCATCTGTTTTACTGCAACTGGCTTAACTATTACGTGT
ATCAGGAAACTCCGTTCAGCCTTACCGCCATCGCTCAAAACCGCGAAGCGCGGGCTGGCGCATAG

Upstream 100 bases:

>100_bases
GGGCGGGTCCCGCCCGCTTGCGGCCGGGACCCGCCAATGGTGCGACAGCGTTAACATTACGCTGTACCCGCATTGCAACT
TTAGTGTCAAGGAAGAGGAT

Downstream 100 bases:

>100_bases
CCTAATCCGGCCAATTGCCGAAACGAGGAGCCTTATGCGATTTTCGACCAGAACCCGTTATGGATTGCGTTTTCTGCTGC
GTCTGGCAGCCCAGCCCCAG

Product: Homoserine O-succinyltransferase

Products: NA

Alternate protein names: Homoserine O-transsuccinylase; HTS

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MPIKIPADLPACAALESENIFVMTEDRAVAQDIRPLEIVIVNLMPTKIATETQLLRLLGNSPLQVNITLLRTEAHESKNT
PAQHLERFYKTFNEIRHGSFDGMIVTGAPVEHLPFEDVDYWHELLDIMNYAASHVYSTLYICWAAQAALYHFYGIPKFSL
PQKISGIFNHDILLPDCRLFRGFDDTFTAPHSRNTEVRAGHILETPQLRLLAQSEQAGVTLVERVDHSQVFMTGHLEYDR
GTLDAEYRRDVDRGLKPRVPEHYYPHDDPAAMPRMNWRAHAHLFYCNWLNYYVYQETPFSLTAIAQNREARAGA

Sequences:

>Translated_314_residues
MPIKIPADLPACAALESENIFVMTEDRAVAQDIRPLEIVIVNLMPTKIATETQLLRLLGNSPLQVNITLLRTEAHESKNT
PAQHLERFYKTFNEIRHGSFDGMIVTGAPVEHLPFEDVDYWHELLDIMNYAASHVYSTLYICWAAQAALYHFYGIPKFSL
PQKISGIFNHDILLPDCRLFRGFDDTFTAPHSRNTEVRAGHILETPQLRLLAQSEQAGVTLVERVDHSQVFMTGHLEYDR
GTLDAEYRRDVDRGLKPRVPEHYYPHDDPAAMPRMNWRAHAHLFYCNWLNYYVYQETPFSLTAIAQNREARAGA
>Mature_313_residues
PIKIPADLPACAALESENIFVMTEDRAVAQDIRPLEIVIVNLMPTKIATETQLLRLLGNSPLQVNITLLRTEAHESKNTP
AQHLERFYKTFNEIRHGSFDGMIVTGAPVEHLPFEDVDYWHELLDIMNYAASHVYSTLYICWAAQAALYHFYGIPKFSLP
QKISGIFNHDILLPDCRLFRGFDDTFTAPHSRNTEVRAGHILETPQLRLLAQSEQAGVTLVERVDHSQVFMTGHLEYDRG
TLDAEYRRDVDRGLKPRVPEHYYPHDDPAAMPRMNWRAHAHLFYCNWLNYYVYQETPFSLTAIAQNREARAGA

Specific function: Methionine biosynthesis; HTS variant; first step. [C]

COG id: COG1897

COG function: function code E; Homoserine trans-succinylase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HTS family

Homologues:

Organism=Escherichia coli, GI1790443, Length=301, Percent_Identity=51.8272425249169, Blast_Score=330, Evalue=7e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): META_DESDA (B8IZQ7)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002479662.1
- ProteinModelPortal:   B8IZQ7
- GeneID:   7284760
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_1078
- HOGENOM:   HBG362506
- GO:   GO:0005737
- HAMAP:   MF_00295
- InterPro:   IPR005697
- PANTHER:   PTHR20919
- PIRSF:   PIRSF000450
- ProDom:   PD037892
- TIGRFAMs:   TIGR01001

Pfam domain/function: PF04204 HTS

EC number: =2.3.1.46

Molecular weight: Translated: 36034; Mature: 35903

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: ACT_SITE 142-142

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIKIPADLPACAALESENIFVMTEDRAVAQDIRPLEIVIVNLMPTKIATETQLLRLLGN
CCEECCCCCCHHHEECCCCEEEEECCCHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHCC
SPLQVNITLLRTEAHESKNTPAQHLERFYKTFNEIRHGSFDGMIVTGAPVEHLPFEDVDY
CCEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHH
WHELLDIMNYAASHVYSTLYICWAAQAALYHFYGIPKFSLPQKISGIFNHDILLPDCRLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCEECCCHHHH
RGFDDTFTAPHSRNTEVRAGHILETPQLRLLAQSEQAGVTLVERVDHSQVFMTGHLEYDR
CCCCCCCCCCCCCCCCCCCCEEECCCCEEEEECCCCCCCHHHHHCCCCEEEEEEEEEECC
GTLDAEYRRDVDRGLKPRVPEHYYPHDDPAAMPRMNWRAHAHLFYCNWLNYYVYQETPFS
CCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEEEEEEEECCCCE
LTAIAQNREARAGA
EEEEECCCCCCCCC
>Mature Secondary Structure 
PIKIPADLPACAALESENIFVMTEDRAVAQDIRPLEIVIVNLMPTKIATETQLLRLLGN
CEECCCCCCHHHEECCCCEEEEECCCHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHCC
SPLQVNITLLRTEAHESKNTPAQHLERFYKTFNEIRHGSFDGMIVTGAPVEHLPFEDVDY
CCEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHH
WHELLDIMNYAASHVYSTLYICWAAQAALYHFYGIPKFSLPQKISGIFNHDILLPDCRLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCEECCCHHHH
RGFDDTFTAPHSRNTEVRAGHILETPQLRLLAQSEQAGVTLVERVDHSQVFMTGHLEYDR
CCCCCCCCCCCCCCCCCCCCEEECCCCEEEEECCCCCCCHHHHHCCCCEEEEEEEEEECC
GTLDAEYRRDVDRGLKPRVPEHYYPHDDPAAMPRMNWRAHAHLFYCNWLNYYVYQETPFS
CCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEEEEEEEECCCCE
LTAIAQNREARAGA
EEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA