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The map label for this gene is nifU [H]

Identifier: 220904348

GI number: 220904348

Start: 1262754

End: 1263590

Strand: Reverse

Name: nifU [H]

Synonym: Ddes_1076

Alternate gene names: 220904348

Gene position: 1263590-1262754 (Counterclockwise)

Preceding gene: 220904349

Following gene: 220904347

Centisome position: 43.97

GC content: 56.87

Gene sequence:

>837_bases
ATGTGGAATTACACTGAAGCCGTTCATGACCATTTTCTGCGCCCGCACAACGTGGGACCCCTGGAAGGAGCCAATGCCAT
TGGCGAGGTGGGCAGCCTGGCCTGTGGCGACGCTCTCAAACTCTATCTCAAGATCAGCGACAAGGGCATTATCGAAAATG
CCACCTTTGAAACATTTGGCTGCGCCAGCGCCATTGCCTCCAGCTCAGTGCTCACGGATATGATCAAGGGTATGTCTGTT
GAAGACGCTCTCAAGGTCAGCAACAAGGACATAGCCGATGCCCTGGGCGGTCTGCCCAAGCAGAAAATGCACTGCTCCGT
CATGGGACAGGAAGCGCTGGAGGCCGCCATCCGCCAGTGGAAGGGCGAGCCGCCCGTGCCCCATGCGCATGAAGAAGGCA
AGCTGGTCTGTAAATGTTTTGGTGTCACCGATGCACAGATCATCCGCGCCATTCGCGAAAACGGCCTCAAGACCGTAGAA
GAAATCACCAACTACACCAAGGCCGGCGGAGCCTGCGGCGAATGCCTGGATGAAATCGGCGAAATCCTTGCTGCCGAACT
CAAGCAAAAGCCACTGGTGGAACTGAAGCCCAAGCCGCGCATGACCAACGTGCAACGCATGCAGCTGGTGCTCAAGACCA
TAGAAGAAGAAATCCGCCCCGTGCTTGCTGCCGATGGTGGCGATATCGAGCTGGTGGATGTGGACGGCTTGCGGGTAGTA
GTGTCCCTGCGCGGCCGTTGCTCGCAGTGTCGCGCCAGCGATGTAACCATTCGCGACCTCGTGCAGCGCCTCTTGCGTGA
ACATGTTGAACCAGATATTGTAGTTGAAGAGGCATAG

Upstream 100 bases:

>100_bases
TTCTGGAGCGAACTTGACGGACACATACGCAGCTTTCTGCAGCAAAGAACCCTGCAGGAAATCATAGAATCAGAGAAGCA
CTCCGCTTCGGGAGGCAATT

Downstream 100 bases:

>100_bases
GGACATGAGAACCATTTATCTCGACAACAATGCCACCACTGCTGTAGCTCCCGAAGTGCGGGACGCCATACTGCCCTATC
TTTCCGAACTGTATGGCAAC

Product: Fe-S cluster assembly protein NifU

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MWNYTEAVHDHFLRPHNVGPLEGANAIGEVGSLACGDALKLYLKISDKGIIENATFETFGCASAIASSSVLTDMIKGMSV
EDALKVSNKDIADALGGLPKQKMHCSVMGQEALEAAIRQWKGEPPVPHAHEEGKLVCKCFGVTDAQIIRAIRENGLKTVE
EITNYTKAGGACGECLDEIGEILAAELKQKPLVELKPKPRMTNVQRMQLVLKTIEEEIRPVLAADGGDIELVDVDGLRVV
VSLRGRCSQCRASDVTIRDLVQRLLREHVEPDIVVEEA

Sequences:

>Translated_278_residues
MWNYTEAVHDHFLRPHNVGPLEGANAIGEVGSLACGDALKLYLKISDKGIIENATFETFGCASAIASSSVLTDMIKGMSV
EDALKVSNKDIADALGGLPKQKMHCSVMGQEALEAAIRQWKGEPPVPHAHEEGKLVCKCFGVTDAQIIRAIRENGLKTVE
EITNYTKAGGACGECLDEIGEILAAELKQKPLVELKPKPRMTNVQRMQLVLKTIEEEIRPVLAADGGDIELVDVDGLRVV
VSLRGRCSQCRASDVTIRDLVQRLLREHVEPDIVVEEA
>Mature_278_residues
MWNYTEAVHDHFLRPHNVGPLEGANAIGEVGSLACGDALKLYLKISDKGIIENATFETFGCASAIASSSVLTDMIKGMSV
EDALKVSNKDIADALGGLPKQKMHCSVMGQEALEAAIRQWKGEPPVPHAHEEGKLVCKCFGVTDAQIIRAIRENGLKTVE
EITNYTKAGGACGECLDEIGEILAAELKQKPLVELKPKPRMTNVQRMQLVLKTIEEEIRPVLAADGGDIELVDVDGLRVV
VSLRGRCSQCRASDVTIRDLVQRLLREHVEPDIVVEEA

Specific function: May be involved in the formation or repair of [Fe-S] clusters present in iron-sulfur proteins (Potential) [H]

COG id: COG0822

COG function: function code C; NifU homolog involved in Fe-S cluster formation

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifU family [H]

Homologues:

Organism=Homo sapiens, GI56699456, Length=121, Percent_Identity=46.2809917355372, Blast_Score=109, Evalue=2e-24,
Organism=Homo sapiens, GI24307953, Length=122, Percent_Identity=45.0819672131148, Blast_Score=107, Evalue=1e-23,
Organism=Escherichia coli, GI1788878, Length=123, Percent_Identity=46.3414634146341, Blast_Score=117, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17543474, Length=121, Percent_Identity=44.6280991735537, Blast_Score=104, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6324800, Length=131, Percent_Identity=46.5648854961832, Blast_Score=118, Evalue=9e-28,
Organism=Saccharomyces cerevisiae, GI6325122, Length=128, Percent_Identity=42.96875, Blast_Score=112, Evalue=7e-26,
Organism=Drosophila melanogaster, GI21355597, Length=121, Percent_Identity=45.4545454545455, Blast_Score=106, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR016217
- InterPro:   IPR010238
- InterPro:   IPR001075
- InterPro:   IPR002871
- ProDom:   PD002830 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01106 NifU; PF01592 NifU_N [H]

EC number: NA

Molecular weight: Translated: 30232; Mature: 30232

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWNYTEAVHDHFLRPHNVGPLEGANAIGEVGSLACGDALKLYLKISDKGIIENATFETFG
CCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCHHEEEEEEECCCCCCCCCCCHHHH
CASAIASSSVLTDMIKGMSVEDALKVSNKDIADALGGLPKQKMHCSVMGQEALEAAIRQW
HHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHC
KGEPPVPHAHEEGKLVCKCFGVTDAQIIRAIRENGLKTVEEITNYTKAGGACGECLDEIG
CCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHH
EILAAELKQKPLVELKPKPRMTNVQRMQLVLKTIEEEIRPVLAADGGDIELVDVDGLRVV
HHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEE
VSLRGRCSQCRASDVTIRDLVQRLLREHVEPDIVVEEA
HHHHCCHHHHCCCCCCHHHHHHHHHHHHCCCCEEEECC
>Mature Secondary Structure
MWNYTEAVHDHFLRPHNVGPLEGANAIGEVGSLACGDALKLYLKISDKGIIENATFETFG
CCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCHHEEEEEEECCCCCCCCCCCHHHH
CASAIASSSVLTDMIKGMSVEDALKVSNKDIADALGGLPKQKMHCSVMGQEALEAAIRQW
HHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHC
KGEPPVPHAHEEGKLVCKCFGVTDAQIIRAIRENGLKTVEEITNYTKAGGACGECLDEIG
CCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHH
EILAAELKQKPLVELKPKPRMTNVQRMQLVLKTIEEEIRPVLAADGGDIELVDVDGLRVV
HHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEE
VSLRGRCSQCRASDVTIRDLVQRLLREHVEPDIVVEEA
HHHHCCHHHHCCCCCCHHHHHHHHHHHHCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7496536 [H]