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The map label for this gene is nifS1 [H]

Identifier: 220904347

GI number: 220904347

Start: 1261595

End: 1262749

Strand: Reverse

Name: nifS1 [H]

Synonym: Ddes_1075

Alternate gene names: 220904347

Gene position: 1262749-1261595 (Counterclockwise)

Preceding gene: 220904348

Following gene: 220904346

Centisome position: 43.95

GC content: 57.49

Gene sequence:

>1155_bases
ATGAGAACCATTTATCTCGACAACAATGCCACCACTGCTGTAGCTCCCGAAGTGCGGGACGCCATACTGCCCTATCTTTC
CGAACTGTATGGCAACCCTTCAAGCATGCACACCTTCGGCGGACAGGTGGCCGAGGCGATGGAAAATGCCAGGCAGAGCC
TGGCCAGCCTTCTTGGCGCCAGCCCCGATGAAATCCTGTTCACCTCGTGCGGCTCGGAAAGCGACAGCTCCGCTATCTGG
ACGGCCCTGCAGACCCAGCCCGATAGACGTCACATCATCACCACCCGCGTTGAACATCCTGCCGTGCTTAACGTTATACA
GCACTGGGAGCGACACGGATACCGCGTCACCTACCTTGCCGTGGACAACAAGGGCAGACTCGACATAGACGAATACGAAC
AGGCCCTGTCCGGCGACACGGCACTTGTATCCATCATGTTTGCCAACAATGAGGTCGGCAACATTTATCCCATACAGCGC
ATGGCCGAAATGGCGCATGAAAAAGGCATACTCTTTCATACCGATGCCGTACAGGCTGTGGGCAAAACCCCCATAGACCT
TGAGCATCTGCCTGCCGACCTGCTCTCGCTCTCCGGCCACAAGCTGCATGCGCCCAAGGGCATAGGCGCCCTGTACATCC
GCAAGGGAGTACGCTTCCGCCCCTTCTTGCGTGGCGGGCATCAGGAACGCGGGCGCCGGGCAGGCACAGAAAACGTACCC
TATATCGTGGGCATGGGCGTGGCTGCCCAGCTGGCCCTGGATCATATGCAGGAAGAGCGCGTACAGGTAGCCATGTTGCG
CGACAAGCTTGAAGCCGGTATTCTGGAACGCATCCCGGACTGCAGGGTCAATGGCGATGTGGAAAACCGCCTACCCAACA
CCACAAACATCGCCTTCAAAAATGTGGAAGGAGAGGCTATCCTGCTCATGCTGGACAGGCTGGGCATCTGCGCAAGCTCC
GGCTCGGCCTGTACTTCGGGCAGTCTTGAGCCTTCGCATGTGCTCAGGGCCATGGGTGTGCCCTTTAATTACGCCCACGG
CTCTGTCCGGCTCTCCCTGTCACGCTACACCACCGAAGATGACGTAAACTTTGTTATCGAGCACTTCCCCCAGGTTATCA
AAACTCTCAGGGCCATATCGCCCTTCAAAGACTAA

Upstream 100 bases:

>100_bases
TCGCAGTGTCGCGCCAGCGATGTAACCATTCGCGACCTCGTGCAGCGCCTCTTGCGTGAACATGTTGAACCAGATATTGT
AGTTGAAGAGGCATAGGGAC

Downstream 100 bases:

>100_bases
CAGTTATCTGATAACAACAAGACAGCACAAACGGGTTACTCCCCGCCGGGCACTGGAAGAATTCCAGACAAGCCCCGGCG
GGGCTTCGTCAGACTCCAAG

Product: cysteine desulfurase NifS

Products: NA

Alternate protein names: Nitrogenase metalloclusters biosynthesis protein nifS1 [H]

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MRTIYLDNNATTAVAPEVRDAILPYLSELYGNPSSMHTFGGQVAEAMENARQSLASLLGASPDEILFTSCGSESDSSAIW
TALQTQPDRRHIITTRVEHPAVLNVIQHWERHGYRVTYLAVDNKGRLDIDEYEQALSGDTALVSIMFANNEVGNIYPIQR
MAEMAHEKGILFHTDAVQAVGKTPIDLEHLPADLLSLSGHKLHAPKGIGALYIRKGVRFRPFLRGGHQERGRRAGTENVP
YIVGMGVAAQLALDHMQEERVQVAMLRDKLEAGILERIPDCRVNGDVENRLPNTTNIAFKNVEGEAILLMLDRLGICASS
GSACTSGSLEPSHVLRAMGVPFNYAHGSVRLSLSRYTTEDDVNFVIEHFPQVIKTLRAISPFKD

Sequences:

>Translated_384_residues
MRTIYLDNNATTAVAPEVRDAILPYLSELYGNPSSMHTFGGQVAEAMENARQSLASLLGASPDEILFTSCGSESDSSAIW
TALQTQPDRRHIITTRVEHPAVLNVIQHWERHGYRVTYLAVDNKGRLDIDEYEQALSGDTALVSIMFANNEVGNIYPIQR
MAEMAHEKGILFHTDAVQAVGKTPIDLEHLPADLLSLSGHKLHAPKGIGALYIRKGVRFRPFLRGGHQERGRRAGTENVP
YIVGMGVAAQLALDHMQEERVQVAMLRDKLEAGILERIPDCRVNGDVENRLPNTTNIAFKNVEGEAILLMLDRLGICASS
GSACTSGSLEPSHVLRAMGVPFNYAHGSVRLSLSRYTTEDDVNFVIEHFPQVIKTLRAISPFKD
>Mature_384_residues
MRTIYLDNNATTAVAPEVRDAILPYLSELYGNPSSMHTFGGQVAEAMENARQSLASLLGASPDEILFTSCGSESDSSAIW
TALQTQPDRRHIITTRVEHPAVLNVIQHWERHGYRVTYLAVDNKGRLDIDEYEQALSGDTALVSIMFANNEVGNIYPIQR
MAEMAHEKGILFHTDAVQAVGKTPIDLEHLPADLLSLSGHKLHAPKGIGALYIRKGVRFRPFLRGGHQERGRRAGTENVP
YIVGMGVAAQLALDHMQEERVQVAMLRDKLEAGILERIPDCRVNGDVENRLPNTTNIAFKNVEGEAILLMLDRLGICASS
GSACTSGSLEPSHVLRAMGVPFNYAHGSVRLSLSRYTTEDDVNFVIEHFPQVIKTLRAISPFKD

Specific function: Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Seems to participate in the biosynthesis of the nitrogenase metalloclusters by providing the inorganic sulfur required for the Fe-S core formation [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=386, Percent_Identity=44.300518134715, Blast_Score=337, Evalue=2e-92,
Organism=Homo sapiens, GI156713448, Length=413, Percent_Identity=34.6246973365617, Blast_Score=219, Evalue=3e-57,
Organism=Escherichia coli, GI48994898, Length=385, Percent_Identity=46.7532467532467, Blast_Score=329, Evalue=2e-91,
Organism=Escherichia coli, GI1787970, Length=403, Percent_Identity=26.0545905707196, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1789175, Length=400, Percent_Identity=24, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI25143064, Length=384, Percent_Identity=42.96875, Blast_Score=321, Evalue=3e-88,
Organism=Caenorhabditis elegans, GI17533177, Length=318, Percent_Identity=33.0188679245283, Blast_Score=169, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6319831, Length=385, Percent_Identity=42.5974025974026, Blast_Score=311, Evalue=1e-85,
Organism=Drosophila melanogaster, GI20129463, Length=385, Percent_Identity=42.5974025974026, Blast_Score=318, Evalue=3e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 42334; Mature: 42334

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTIYLDNNATTAVAPEVRDAILPYLSELYGNPSSMHTFGGQVAEAMENARQSLASLLGA
CEEEEECCCCCEEECHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
SPDEILFTSCGSESDSSAIWTALQTQPDRRHIITTRVEHPAVLNVIQHWERHGYRVTYLA
CCCCEEEEECCCCCCCCHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEE
VDNKGRLDIDEYEQALSGDTALVSIMFANNEVGNIYPIQRMAEMAHEKGILFHTDAVQAV
ECCCCCCCHHHHHHHHCCCCEEEEEEEECCCCCCEECHHHHHHHHHHCCCEEECHHHHHH
GKTPIDLEHLPADLLSLSGHKLHAPKGIGALYIRKGVRFRPFLRGGHQERGRRAGTENVP
CCCCCCHHHCCHHHHHCCCCEEECCCCCCCCEECCCCCCCHHHCCCCHHHHCCCCCCCCC
YIVGMGVAAQLALDHMQEERVQVAMLRDKLEAGILERIPDCRVNGDVENRLPNTTNIAFK
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCEEEE
NVEGEAILLMLDRLGICASSGSACTSGSLEPSHVLRAMGVPFNYAHGSVRLSLSRYTTED
CCCCCEEEEEEHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCEEECCCEEEEEEECCCCCC
DVNFVIEHFPQVIKTLRAISPFKD
HHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRTIYLDNNATTAVAPEVRDAILPYLSELYGNPSSMHTFGGQVAEAMENARQSLASLLGA
CEEEEECCCCCEEECHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
SPDEILFTSCGSESDSSAIWTALQTQPDRRHIITTRVEHPAVLNVIQHWERHGYRVTYLA
CCCCEEEEECCCCCCCCHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEE
VDNKGRLDIDEYEQALSGDTALVSIMFANNEVGNIYPIQRMAEMAHEKGILFHTDAVQAV
ECCCCCCCHHHHHHHHCCCCEEEEEEEECCCCCCEECHHHHHHHHHHCCCEEECHHHHHH
GKTPIDLEHLPADLLSLSGHKLHAPKGIGALYIRKGVRFRPFLRGGHQERGRRAGTENVP
CCCCCCHHHCCHHHHHCCCCEEECCCCCCCCEECCCCCCCHHHCCCCHHHHCCCCCCCCC
YIVGMGVAAQLALDHMQEERVQVAMLRDKLEAGILERIPDCRVNGDVENRLPNTTNIAFK
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCEEEE
NVEGEAILLMLDRLGICASSGSACTSGSLEPSHVLRAMGVPFNYAHGSVRLSLSRYTTED
CCCCCEEEEEEHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCEEECCCEEEEEEECCCCCC
DVNFVIEHFPQVIKTLRAISPFKD
HHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7568132 [H]