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The map label for this gene is greA

Identifier: 220904317

GI number: 220904317

Start: 1224388

End: 1224876

Strand: Reverse

Name: greA

Synonym: Ddes_1044

Alternate gene names: 220904317

Gene position: 1224876-1224388 (Counterclockwise)

Preceding gene: 220904319

Following gene: 220904315

Centisome position: 42.63

GC content: 56.03

Gene sequence:

>489_bases
ATGACGAGTATTCCCATTTCCGTACAAGGCTACAAGGCGCTGGAAGAAGAATTGGCCCGCCTGAAAAGCGAACGGCCGGA
GATCATTCAAGCCATCAAGGAAGCCAGAGAAGAAGGCGACCTGCGCGAAAATGCCGGCTATGACGCCGCGCGCGAACGCC
AGGGCATGGCTGAGGCGCGTATAAAGTACATCGAGTCGCGCATGGCCCTGTATCAGGTCATTGACCTTGATACCCTCAGC
GGCGACAAGGTCATCTTTGGCGCAACTGTGGACCTGGAAGATGTAGATAGCGGCGAAGCCAAAACCTACACCATTCTTGG
CCCGGATGAGGCCGACCCCAGCAAGGGATCCATTTCTTTTCTTTCACCCGTGGGGCAGGCCCTGCTCGGGCGCGAGGAAG
GGGACGAAGTTTCCGTTGAAATTCCTCGTGGCCGTGTCACTTACGAAATTATCGGCGTCAGCTTCAAGGGCAGCAAAAAC
CTCGGCTAA

Upstream 100 bases:

>100_bases
CTGCCGGAATACCAGATTTTTTTTCATAAAAACCACTTTTTGGTAAACGCATGCTCCGCTGCCTGGGCAGCGGCGTTTTT
TTTGCGTTGCGAGGTTCGAC

Downstream 100 bases:

>100_bases
GGCAATATTGCCCCAACATTGCATGGCGGCGGCTGCGCGGGCGGCACCCGGCACAATTGGTGAGCGAAGTTTGCCTGCGC
GACCAGACGCCCGTGTGGGT

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA

Number of amino acids: Translated: 162; Mature: 161

Protein sequence:

>162_residues
MTSIPISVQGYKALEEELARLKSERPEIIQAIKEAREEGDLRENAGYDAARERQGMAEARIKYIESRMALYQVIDLDTLS
GDKVIFGATVDLEDVDSGEAKTYTILGPDEADPSKGSISFLSPVGQALLGREEGDEVSVEIPRGRVTYEIIGVSFKGSKN
LG

Sequences:

>Translated_162_residues
MTSIPISVQGYKALEEELARLKSERPEIIQAIKEAREEGDLRENAGYDAARERQGMAEARIKYIESRMALYQVIDLDTLS
GDKVIFGATVDLEDVDSGEAKTYTILGPDEADPSKGSISFLSPVGQALLGREEGDEVSVEIPRGRVTYEIIGVSFKGSKN
LG
>Mature_161_residues
TSIPISVQGYKALEEELARLKSERPEIIQAIKEAREEGDLRENAGYDAARERQGMAEARIKYIESRMALYQVIDLDTLSG
DKVIFGATVDLEDVDSGEAKTYTILGPDEADPSKGSISFLSPVGQALLGREEGDEVSVEIPRGRVTYEIIGVSFKGSKNL
G

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family

Homologues:

Organism=Escherichia coli, GI87082225, Length=157, Percent_Identity=50.3184713375796, Blast_Score=145, Evalue=1e-36,
Organism=Escherichia coli, GI87082261, Length=156, Percent_Identity=31.4102564102564, Blast_Score=81, Evalue=3e-17,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): GREA_DESDA (B8IZM4)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002479629.1
- GeneID:   7284725
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_1044
- HOGENOM:   HBG753387
- ProtClustDB:   PRK00226
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01462

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 17697; Mature: 17566

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSIPISVQGYKALEEELARLKSERPEIIQAIKEAREEGDLRENAGYDAARERQGMAEAR
CCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCHHHHH
IKYIESRMALYQVIDLDTLSGDKVIFGATVDLEDVDSGEAKTYTILGPDEADPSKGSISF
HHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCCHH
LSPVGQALLGREEGDEVSVEIPRGRVTYEIIGVSFKGSKNLG
HHHHHHHHHCCCCCCEEEEEECCCEEEEEEEEEEECCCCCCC
>Mature Secondary Structure 
TSIPISVQGYKALEEELARLKSERPEIIQAIKEAREEGDLRENAGYDAARERQGMAEAR
CCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCHHHHH
IKYIESRMALYQVIDLDTLSGDKVIFGATVDLEDVDSGEAKTYTILGPDEADPSKGSISF
HHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCCHH
LSPVGQALLGREEGDEVSVEIPRGRVTYEIIGVSFKGSKNLG
HHHHHHHHHCCCCCCEEEEEECCCEEEEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA