Click here to switch to the map view.

The map label for this gene is ydiS [H]

Identifier: 218902127

GI number: 218902127

Start: 977268

End: 978809

Strand: Direct

Name: ydiS [H]

Synonym: BCAH820_1009

Alternate gene names: 218902127

Gene position: 977268-978809 (Clockwise)

Preceding gene: 218902126

Following gene: 218902128

Centisome position: 18.43

GC content: 32.75

Gene sequence:

>1542_bases
ATGAATTATACAATTAAAACCAGTAATCCAGAGACAGTAAACGACATTTTAGAGAAAAAAAGTGGAGTAGAAATAACAAA
TTTAAATAACTTCCCTAATGATTTAAAGTTAAACGAAACGGTTCTTATCGTTCTTGGTGGGGATAAGGTGGAATGGGAAA
AAGGGTTCATAGGATTTGGTCATATTACAACGTTACCTTATGATTTTGAAGGTAGAAATTATAAAGTTAAAATTGATGTG
GATTTTTTATTAGAGAACCCAATGACTAAATATGATTTGCGATTTTATCCTGGCATTTTTAATGTGACGTTTATAGGACC
TATGACTAAAGGGGAGCCTAACCAAGCAATTCAGAGAATAGAATTAAGTAATATAAAAGTTTTATTAAGAGCTATATTAG
ATTTAGATGCACAGCATGAAGAAGCTCTTACTCGCATTTTTGGCGAAGAGACAATGGAAGAGTGTAAACAAAGGATTGAA
TATTTAGAGACGCAATATAGGTCTTATAGTGAAAAACAACAAGTAATGGAACAAAGTGATGTAATAAAAGAGGAAGAAGA
GGTAATTAATTTTCTTAGAGAAGAAGAGTTTGTAACAGGTGGTCATAATTTAATAGTATATGGTGCCCCTGGTACAGGGA
AAAGTTATGAGCTAAGTCAGAGATATAAAAATATAGAAAGAGTGGTTTTCCATCCGGAATATACGTATCATGATTTCGTA
GGTTCATATAAGCCGGTTCCTTTATACAAAAAAATTGATGGGAATATCCTTAGGGATTCAGCGGGAAGAGAGTATTCTAT
TGGAGAACCGATAATTGATTATCAGTTTGTGCCTGGTCCATTCACTAAAGTCTTAATTAAAGCATTTAAACAACCTCATA
TCCTCCATACACTAGTCATTGAGGAAATTAATAGGGCAAACACAGCAGCTGTTTTTGGGGACATATTCCAACTTTTAGAT
AGAGATGCTAATGGTAAGAGTGAATATGAAATAGAAATAAATCAGGATCTGTGTAATTACCTAATTAGACAAGGTGTTTT
ACCAAATCAGGGCTCTTATTCACTGTTTATTCCGTCCAATTTAAATATTGTAGCTACAATGAATAGTGCAGATCAGGGAG
TCTTTCCTTTAGATTCGGCGTTTAAAAGAAGATGGCGTTTTGAATATCTTCCAATTAACATTGATGATGCCTTCCATGCG
AACGAAGAAATCTGCTATGGTGATATGAAAGTGACATGGGGACAATTGGTTAAAAATATAAATCGTGTTCTGACTGTAAA
TGGTGTAAATGAAGACAAGCATATAGGTCCATATTTCCTGACACAAATAGACTTGAAAGATCAAAAAATATTCTCTTCTA
AATTACTAGTATATTTATGGGATGATGTGCTTAGACATAATCGAGCAATTTTATTTAAAAGTGCAAGGACATTTTCAGAA
TTGGTACAGAGTTATGAAAAGGGAGAGGAGATATTCACCTTTAATATAAATGATGTTTTAGATATTCAAGAAAGTGAATT
TGTTAGTGATGTTGAATATTAA

Upstream 100 bases:

>100_bases
AAAGAACTTCTTAGAGGTTCTTTTTTATTTTGTTAGATTACATCATATTTAATATAGTAAAATTATAATTAGTCAATATT
TTGAGTAGGTGGAGGAGAAA

Downstream 100 bases:

>100_bases
GGATCATTGTTTTATCGAGAATGTAAAATACAGTACGAAGAAGTTGAAAGTAAATTTAGGAGTCAATATTTTTAAAGCAG
GCCTATGTGAAGAAGTAAAT

Product: type II restriction-modification system restriction subunit

Products: NA

Alternate protein names: R.BsuM; R.BsuMI; Endonuclease BsuMI component ydiS; Type II restriction enzyme BsuMI component ydiS [H]

Number of amino acids: Translated: 513; Mature: 513

Protein sequence:

>513_residues
MNYTIKTSNPETVNDILEKKSGVEITNLNNFPNDLKLNETVLIVLGGDKVEWEKGFIGFGHITTLPYDFEGRNYKVKIDV
DFLLENPMTKYDLRFYPGIFNVTFIGPMTKGEPNQAIQRIELSNIKVLLRAILDLDAQHEEALTRIFGEETMEECKQRIE
YLETQYRSYSEKQQVMEQSDVIKEEEEVINFLREEEFVTGGHNLIVYGAPGTGKSYELSQRYKNIERVVFHPEYTYHDFV
GSYKPVPLYKKIDGNILRDSAGREYSIGEPIIDYQFVPGPFTKVLIKAFKQPHILHTLVIEEINRANTAAVFGDIFQLLD
RDANGKSEYEIEINQDLCNYLIRQGVLPNQGSYSLFIPSNLNIVATMNSADQGVFPLDSAFKRRWRFEYLPINIDDAFHA
NEEICYGDMKVTWGQLVKNINRVLTVNGVNEDKHIGPYFLTQIDLKDQKIFSSKLLVYLWDDVLRHNRAILFKSARTFSE
LVQSYEKGEEIFTFNINDVLDIQESEFVSDVEY

Sequences:

>Translated_513_residues
MNYTIKTSNPETVNDILEKKSGVEITNLNNFPNDLKLNETVLIVLGGDKVEWEKGFIGFGHITTLPYDFEGRNYKVKIDV
DFLLENPMTKYDLRFYPGIFNVTFIGPMTKGEPNQAIQRIELSNIKVLLRAILDLDAQHEEALTRIFGEETMEECKQRIE
YLETQYRSYSEKQQVMEQSDVIKEEEEVINFLREEEFVTGGHNLIVYGAPGTGKSYELSQRYKNIERVVFHPEYTYHDFV
GSYKPVPLYKKIDGNILRDSAGREYSIGEPIIDYQFVPGPFTKVLIKAFKQPHILHTLVIEEINRANTAAVFGDIFQLLD
RDANGKSEYEIEINQDLCNYLIRQGVLPNQGSYSLFIPSNLNIVATMNSADQGVFPLDSAFKRRWRFEYLPINIDDAFHA
NEEICYGDMKVTWGQLVKNINRVLTVNGVNEDKHIGPYFLTQIDLKDQKIFSSKLLVYLWDDVLRHNRAILFKSARTFSE
LVQSYEKGEEIFTFNINDVLDIQESEFVSDVEY
>Mature_513_residues
MNYTIKTSNPETVNDILEKKSGVEITNLNNFPNDLKLNETVLIVLGGDKVEWEKGFIGFGHITTLPYDFEGRNYKVKIDV
DFLLENPMTKYDLRFYPGIFNVTFIGPMTKGEPNQAIQRIELSNIKVLLRAILDLDAQHEEALTRIFGEETMEECKQRIE
YLETQYRSYSEKQQVMEQSDVIKEEEEVINFLREEEFVTGGHNLIVYGAPGTGKSYELSQRYKNIERVVFHPEYTYHDFV
GSYKPVPLYKKIDGNILRDSAGREYSIGEPIIDYQFVPGPFTKVLIKAFKQPHILHTLVIEEINRANTAAVFGDIFQLLD
RDANGKSEYEIEINQDLCNYLIRQGVLPNQGSYSLFIPSNLNIVATMNSADQGVFPLDSAFKRRWRFEYLPINIDDAFHA
NEEICYGDMKVTWGQLVKNINRVLTVNGVNEDKHIGPYFLTQIDLKDQKIFSSKLLVYLWDDVLRHNRAILFKSARTFSE
LVQSYEKGEEIFTFNINDVLDIQESEFVSDVEY

Specific function: Recognizes the double-stranded sequence CTCGAG; the cleavage site is unknown [H]

COG id: COG1401

COG function: function code V; GTPase subunit of restriction endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011704 [H]

Pfam domain/function: PF07728 AAA_5 [H]

EC number: =3.1.21.4 [H]

Molecular weight: Translated: 59369; Mature: 59369

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYTIKTSNPETVNDILEKKSGVEITNLNNFPNDLKLNETVLIVLGGDKVEWEKGFIGFG
CCEEEECCCCHHHHHHHHHCCCCEEEECCCCCCCCEECCEEEEEECCCEEEECCCEEEEC
HITTLPYDFEGRNYKVKIDVDFLLENPMTKYDLRFYPGIFNVTFIGPMTKGEPNQAIQRI
CCEECCEEECCCEEEEEEEEEEEECCCCCEECEEECCCEEEEEEECCCCCCCHHHHHHHH
ELSNIKVLLRAILDLDAQHEEALTRIFGEETMEECKQRIEYLETQYRSYSEKQQVMEQSD
HHHHHHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VIKEEEEVINFLREEEFVTGGHNLIVYGAPGTGKSYELSQRYKNIERVVFHPEYTYHDFV
HHHHHHHHHHHHHHHHHEECCCEEEEECCCCCCCCCHHHHHHCCCCEEEECCCCCHHHHC
GSYKPVPLYKKIDGNILRDSAGREYSIGEPIIDYQFVPGPFTKVLIKAFKQPHILHTLVI
CCCCCCCEEECCCCCEEECCCCCCCCCCCCCCCEEECCCHHHHHHHHHHCCCHHHHHHHH
EEINRANTAAVFGDIFQLLDRDANGKSEYEIEINQDLCNYLIRQGVLPNQGSYSLFIPSN
HHHCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHHHCCCCCCCCCEEEEECCC
LNIVATMNSADQGVFPLDSAFKRRWRFEYLPINIDDAFHANEEICYGDMKVTWGQLVKNI
CEEEEEECCCCCCCCCCCHHHHHCCCEEEEEEECCCCCCCCCCEEECCCEECHHHHHHHC
NRVLTVNGVNEDKHIGPYFLTQIDLKDQKIFSSKLLVYLWDDVLRHNRAILFKSARTFSE
CEEEEEECCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHH
LVQSYEKGEEIFTFNINDVLDIQESEFVSDVEY
HHHHHHCCCEEEEEECCCEECCCHHHHHHCCCC
>Mature Secondary Structure
MNYTIKTSNPETVNDILEKKSGVEITNLNNFPNDLKLNETVLIVLGGDKVEWEKGFIGFG
CCEEEECCCCHHHHHHHHHCCCCEEEECCCCCCCCEECCEEEEEECCCEEEECCCEEEEC
HITTLPYDFEGRNYKVKIDVDFLLENPMTKYDLRFYPGIFNVTFIGPMTKGEPNQAIQRI
CCEECCEEECCCEEEEEEEEEEEECCCCCEECEEECCCEEEEEEECCCCCCCHHHHHHHH
ELSNIKVLLRAILDLDAQHEEALTRIFGEETMEECKQRIEYLETQYRSYSEKQQVMEQSD
HHHHHHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VIKEEEEVINFLREEEFVTGGHNLIVYGAPGTGKSYELSQRYKNIERVVFHPEYTYHDFV
HHHHHHHHHHHHHHHHHEECCCEEEEECCCCCCCCCHHHHHHCCCCEEEECCCCCHHHHC
GSYKPVPLYKKIDGNILRDSAGREYSIGEPIIDYQFVPGPFTKVLIKAFKQPHILHTLVI
CCCCCCCEEECCCCCEEECCCCCCCCCCCCCCCEEECCCHHHHHHHHHHCCCHHHHHHHH
EEINRANTAAVFGDIFQLLDRDANGKSEYEIEINQDLCNYLIRQGVLPNQGSYSLFIPSN
HHHCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHHHCCCCCCCCCEEEEECCC
LNIVATMNSADQGVFPLDSAFKRRWRFEYLPINIDDAFHANEEICYGDMKVTWGQLVKNI
CEEEEEECCCCCCCCCCCHHHHHCCCEEEEEEECCCCCCCCCCEEECCCEECHHHHHHHC
NRVLTVNGVNEDKHIGPYFLTQIDLKDQKIFSSKLLVYLWDDVLRHNRAILFKSARTFSE
CEEEEEECCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHH
LVQSYEKGEEIFTFNINDVLDIQESEFVSDVEY
HHHHHHCCCEEEEEECCCEECCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9455482; 9384377 [H]