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The map label for this gene is bsp6IM [H]

Identifier: 218902126

GI number: 218902126

Start: 976114

End: 977166

Strand: Direct

Name: bsp6IM [H]

Synonym: BCAH820_1008

Alternate gene names: 218902126

Gene position: 976114-977166 (Clockwise)

Preceding gene: 218902125

Following gene: 218902127

Centisome position: 18.41

GC content: 32.86

Gene sequence:

>1053_bases
ATGACTGTAAAGTATAAATTAGGGAGTATGTTTGCCGGAATTGGTGGTACATGCTTGGGATTTCAACAAGCCGGTGCTGA
GATTGTTTGGGCTAATGAAGTGGATCGTAATGCTTCCATCACATACAGACATTTTTGGAAAGGTGAATATTTACAAGAGG
CTGATGTTACAGAGGTGGATAAAACTACGATTCCACAGTTAGACATATTAATTGGTGGCTTTCCATGTCAAGCGTTTAGT
ATTGCAGGTTATCGAAAAGGCTTTGAAGATGAGAGAGGAAATATGTTTTTTCAAATTCTTGACGTAATTGATGCACAAGA
AAAAATGTATGGTACTAAGCCAAAAGCAATTATGCTAGAAAATGTTAAAAATTTAAAAGGTCACGATAAAGGAAATACTT
TTAGGGTTATTAAAGAGTGTCTAGAGGAAAAAGGATATACAATAAAGGATAAGGTTTTAAATTCAATGGAATATGGAAAT
GTTCCGCAAAATAGAGAGAGAATTTACATTGTAGGATTTTTAGAAAATGAATTGACAGCCAAATTCGATTTTCCAGCACC
AATTCCATTAACTAATACACTTAATACAGTTATTAATCGAAATAGTAGTATTAACGATGAGAAATATAAAGCATTTTATT
ACCTTCCAGAAAAGGTTAGTTTTTATAACGAACTTCAAGAAGGAATGACTAGCCATGACACAGTTTATCAATGGCGTAGA
GTATATGTGAGAGAAAATAAAAATAATGTTTGTCCAACACTAACAGCTAATATGGGAACAGGTGGACACAATGTGCCTTT
AATTTTAGATAATGAAAATAATATTAGAAAATTAACACCTAAAGAATGCTTATTACTTCAAGGGTTCCCAGAAGAGTTAA
CTTTGCCAGAAGGAATGGCGAATAGTCATTTGTATAAACAAGCTGGTAATTCAGTTACGGTTCCTGTGATTCGTCGTATT
GCAAACAATATAATTGAAGTATTAAGAGGCAATGAATTAACTGCTGATGTTATAGTACAAGAAGAAAATGATGGACAATT
GACTTTTGTTTAA

Upstream 100 bases:

>100_bases
AACAGAATTATTTCCTAATAATGACTGAAGATTTCTCAATTATTAGGGTGATATAATAAATTAAAAAGAGGATTTTTTTG
GTTAGAAGGAGAGAACTTTA

Downstream 100 bases:

>100_bases
AAAAGAACTTCTTAGAGGTTCTTTTTTATTTTGTTAGATTACATCATATTTAATATAGTAAAATTATAATTAGTCAATAT
TTTGAGTAGGTGGAGGAGAA

Product: cytosine-specific methyltransferase NlaX

Products: NA

Alternate protein names: M.Bsp6I; Cytosine-specific methyltransferase Bsp6I [H]

Number of amino acids: Translated: 350; Mature: 349

Protein sequence:

>350_residues
MTVKYKLGSMFAGIGGTCLGFQQAGAEIVWANEVDRNASITYRHFWKGEYLQEADVTEVDKTTIPQLDILIGGFPCQAFS
IAGYRKGFEDERGNMFFQILDVIDAQEKMYGTKPKAIMLENVKNLKGHDKGNTFRVIKECLEEKGYTIKDKVLNSMEYGN
VPQNRERIYIVGFLENELTAKFDFPAPIPLTNTLNTVINRNSSINDEKYKAFYYLPEKVSFYNELQEGMTSHDTVYQWRR
VYVRENKNNVCPTLTANMGTGGHNVPLILDNENNIRKLTPKECLLLQGFPEELTLPEGMANSHLYKQAGNSVTVPVIRRI
ANNIIEVLRGNELTADVIVQEENDGQLTFV

Sequences:

>Translated_350_residues
MTVKYKLGSMFAGIGGTCLGFQQAGAEIVWANEVDRNASITYRHFWKGEYLQEADVTEVDKTTIPQLDILIGGFPCQAFS
IAGYRKGFEDERGNMFFQILDVIDAQEKMYGTKPKAIMLENVKNLKGHDKGNTFRVIKECLEEKGYTIKDKVLNSMEYGN
VPQNRERIYIVGFLENELTAKFDFPAPIPLTNTLNTVINRNSSINDEKYKAFYYLPEKVSFYNELQEGMTSHDTVYQWRR
VYVRENKNNVCPTLTANMGTGGHNVPLILDNENNIRKLTPKECLLLQGFPEELTLPEGMANSHLYKQAGNSVTVPVIRRI
ANNIIEVLRGNELTADVIVQEENDGQLTFV
>Mature_349_residues
TVKYKLGSMFAGIGGTCLGFQQAGAEIVWANEVDRNASITYRHFWKGEYLQEADVTEVDKTTIPQLDILIGGFPCQAFSI
AGYRKGFEDERGNMFFQILDVIDAQEKMYGTKPKAIMLENVKNLKGHDKGNTFRVIKECLEEKGYTIKDKVLNSMEYGNV
PQNRERIYIVGFLENELTAKFDFPAPIPLTNTLNTVINRNSSINDEKYKAFYYLPEKVSFYNELQEGMTSHDTVYQWRRV
YVRENKNNVCPTLTANMGTGGHNVPLILDNENNIRKLTPKECLLLQGFPEELTLPEGMANSHLYKQAGNSVTVPVIRRIA
NNIIEVLRGNELTADVIVQEENDGQLTFV

Specific function: This methylase recognizes the double-stranded sequence GCNGC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the Bsp6I endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788271, Length=371, Percent_Identity=30.4582210242588, Blast_Score=141, Evalue=6e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 39721; Mature: 39590

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS00094 C5_MTASE_1 ; PS00095 C5_MTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVKYKLGSMFAGIGGTCLGFQQAGAEIVWANEVDRNASITYRHFWKGEYLQEADVTEVD
CEEEEEHHHHHHCCCCCEECHHHCCCEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCC
KTTIPQLDILIGGFPCQAFSIAGYRKGFEDERGNMFFQILDVIDAQEKMYGTKPKAIMLE
CCCCCEEEEEEECCCCHHEEHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEH
NVKNLKGHDKGNTFRVIKECLEEKGYTIKDKVLNSMEYGNVPQNRERIYIVGFLENELTA
HHHHCCCCCCCCHHHHHHHHHHHCCCEEHHHHHHHHHCCCCCCCCCEEEEEEEECCCCEE
KFDFPAPIPLTNTLNTVINRNSSINDEKYKAFYYLPEKVSFYNELQEGMTSHDTVYQWRR
EECCCCCCCCHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHCCCCHHHHHHHHH
VYVRENKNNVCPTLTANMGTGGHNVPLILDNENNIRKLTPKECLLLQGFPEELTLPEGMA
EEEECCCCCCCCEEEECCCCCCCCCCEEEECCCCCEEECHHHEEEECCCCHHCCCCCCCC
NSHLYKQAGNSVTVPVIRRIANNIIEVLRGNELTADVIVQEENDGQLTFV
CCHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCEEEC
>Mature Secondary Structure 
TVKYKLGSMFAGIGGTCLGFQQAGAEIVWANEVDRNASITYRHFWKGEYLQEADVTEVD
EEEEEHHHHHHCCCCCEECHHHCCCEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCC
KTTIPQLDILIGGFPCQAFSIAGYRKGFEDERGNMFFQILDVIDAQEKMYGTKPKAIMLE
CCCCCEEEEEEECCCCHHEEHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEH
NVKNLKGHDKGNTFRVIKECLEEKGYTIKDKVLNSMEYGNVPQNRERIYIVGFLENELTA
HHHHCCCCCCCCHHHHHHHHHHHCCCEEHHHHHHHHHCCCCCCCCCEEEEEEEECCCCEE
KFDFPAPIPLTNTLNTVINRNSSINDEKYKAFYYLPEKVSFYNELQEGMTSHDTVYQWRR
EECCCCCCCCHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHCCCCHHHHHHHHH
VYVRENKNNVCPTLTANMGTGGHNVPLILDNENNIRKLTPKECLLLQGFPEELTLPEGMA
EEEECCCCCCCCEEEECCCCCCCCCCEEEECCCCCEEECHHHEEEECCCCHHCCCCCCCC
NSHLYKQAGNSVTVPVIRRIANNIIEVLRGNELTADVIVQEENDGQLTFV
CCHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7607501 [H]