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The map label for this gene is yfcE [C]

Identifier: 218884150

GI number: 218884150

Start: 808474

End: 809010

Strand: Reverse

Name: yfcE [C]

Synonym: DKAM_0839

Alternate gene names: 218884150

Gene position: 809010-808474 (Counterclockwise)

Preceding gene: 218884151

Following gene: 218884149

Centisome position: 59.26

GC content: 41.15

Gene sequence:

>537_bases
GTGGTGTCCTCGATGATCATTGGTGTATTAAGCGATACACATGACAATATTCATGCAACCAGGAGAATTCTTAGAAGGTT
ATCTGACCATAATGTAGAGCTCATAATCCACCTCGGAGACATTGTAAGTCCGTTCACCCTTAAAGCTATAAGGGAGGAGA
TCGGTGGTGTTAAACTTATTATTGTTAAGGGGAATAACGATGGTGACATATACTTATTGAATAAGCTGTCCTCGGGCTAT
GGATGGATATTCTATAGCGAGCCAACCATACTGGAGATAGGGGGTAGGAGAATATTCATAATGCATGGATACGGAGACGC
ATCTACAACGGAGAAAATAGTGGACGCCCTCGCTAAATCCATCGACACCGATATATTGCTCTACGGGCACACCCATATAG
CTAGGAGTGAACACATAAATGGTAAACTAGTATTAAACCCGGGTGAGGCATGTGGTTACATAACTGGGAGACATACCTAC
GGGATCATAGATACCATTACTTTAAATACTGAAATATTCGAAGAAGATATAAAGTAA

Upstream 100 bases:

>100_bases
AAACTCAAGAAAGCTGGTAAAGGGAAAGAGGGATTCAAGATTAGTGTCGAGTAGTTTCACTTGTGGAACCATGAATAGTT
TTTCACAGCGCTTTTAAACC

Downstream 100 bases:

>100_bases
AATACAAGGGGGCAATGAAATGAGTGAGAGGCTCAAGAGCGCGAGTCGCCCCGAAAGCGAGGAGGGTAGACAATACCATT
TACAAGTGAAGCCGGGGGAT

Product: phosphodiesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 178; Mature: 178

Protein sequence:

>178_residues
MVSSMIIGVLSDTHDNIHATRRILRRLSDHNVELIIHLGDIVSPFTLKAIREEIGGVKLIIVKGNNDGDIYLLNKLSSGY
GWIFYSEPTILEIGGRRIFIMHGYGDASTTEKIVDALAKSIDTDILLYGHTHIARSEHINGKLVLNPGEACGYITGRHTY
GIIDTITLNTEIFEEDIK

Sequences:

>Translated_178_residues
MVSSMIIGVLSDTHDNIHATRRILRRLSDHNVELIIHLGDIVSPFTLKAIREEIGGVKLIIVKGNNDGDIYLLNKLSSGY
GWIFYSEPTILEIGGRRIFIMHGYGDASTTEKIVDALAKSIDTDILLYGHTHIARSEHINGKLVLNPGEACGYITGRHTY
GIIDTITLNTEIFEEDIK
>Mature_178_residues
MVSSMIIGVLSDTHDNIHATRRILRRLSDHNVELIIHLGDIVSPFTLKAIREEIGGVKLIIVKGNNDGDIYLLNKLSSGY
GWIFYSEPTILEIGGRRIFIMHGYGDASTTEKIVDALAKSIDTDILLYGHTHIARSEHINGKLVLNPGEACGYITGRHTY
GIIDTITLNTEIFEEDIK

Specific function: Shows phosphodiesterase activity, hydrolyzing phosphodiesters bonds in the artificial chromogenic substrates bis-p-nitrophenyl phosphate (bis-pNPP), and less efficiently thymidine 5'-monophosphate p-nitrophenyl ester (pNP-TMP) and p- nitrophenylphosphoryl

COG id: COG0622

COG function: function code R; Predicted phosphoesterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallophosphoesterase superfamily. YfcE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR020935
- InterPro:   IPR000979 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: NA

Molecular weight: Translated: 19785; Mature: 19785

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSSMIIGVLSDTHDNIHATRRILRRLSDHNVELIIHLGDIVSPFTLKAIREEIGGVKLI
CCCCEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHCCEEEE
IVKGNNDGDIYLLNKLSSGYGWIFYSEPTILEIGGRRIFIMHGYGDASTTEKIVDALAKS
EEEECCCCCEEEEEECCCCEEEEEECCCEEEEECCEEEEEEECCCCCCHHHHHHHHHHHH
IDTDILLYGHTHIARSEHINGKLVLNPGEACGYITGRHTYGIIDTITLNTEIFEEDIK
CCCEEEEEECCEECCCCCCCCEEEECCCCCCCEEECCCEEEEEEEEEECHHHHHHHCC
>Mature Secondary Structure
MVSSMIIGVLSDTHDNIHATRRILRRLSDHNVELIIHLGDIVSPFTLKAIREEIGGVKLI
CCCCEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHCCEEEE
IVKGNNDGDIYLLNKLSSGYGWIFYSEPTILEIGGRRIFIMHGYGDASTTEKIVDALAKS
EEEECCCCCEEEEEECCCCEEEEEECCCEEEEECCEEEEEEECCCCCCHHHHHHHHHHHH
IDTDILLYGHTHIARSEHINGKLVLNPGEACGYITGRHTYGIIDTITLNTEIFEEDIK
CCCEEEEEECCEECCCCCCCCEEEECCCCCCCEEECCCEEEEEEEEEECHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]