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The map label for this gene is pyrB [H]

Identifier: 218884053

GI number: 218884053

Start: 712864

End: 713787

Strand: Direct

Name: pyrB [H]

Synonym: DKAM_0742

Alternate gene names: 218884053

Gene position: 712864-713787 (Clockwise)

Preceding gene: 218884052

Following gene: 218884056

Centisome position: 52.22

GC content: 46.54

Gene sequence:

>924_bases
ATGTACAAGGGAATGGATGTTATATCAGCCCTCCAGTTCTCCAGGAGCGATGTGGAGAAATTAATCACCATAGCAGAGGA
GTTAAGGAGGCGTGTTGAAAACGGTGAAAAACTAGAACTGGCCAAGGGAAGAATACTTTTCACAGCGTTCTTCGAGCCCA
GCACTAGAACCCGGCTGAGCTTCCAGTTCGCCATGGCTAAGCTAGGGGGCAACGTAATTGATCTAGGACCCGAGGAGGTC
ACGAGTAGAGCCAAGGGAGAGAGCCCTGAGGACACCCTCAGGACAGTTGATAGCTACGATCCCGACATAATAGTTGTGAG
ACATAGGGAACCCGGGTTCGCGGCTAAGGCTGCTGAAATATGCAGAGCCCCAGTGGTGAACGCCGGGGATGGATACAATG
AACACCCAACGCAAGCCCTACTTGATGTCTACACTATATGGAGGAGCTTGGGTAGAATAGATGGGGTAACAATAGGGATA
ATGGGGGATTTAAAGTATGGTAGAACCATCTCTAGCCTATCATATATATTATCCAACTTTAGGGATGTAACAATATACTT
TATCTCACCGCAGGCACTAAGACCCCGTGAAGAAGTATTAAAGATACTTGACCAGAGGAGGACAAGGTACGAACTCCTCG
AAAAACCAGACGATGCAATGGAGAAACTGAATATCCTGTATGTGACAAGACTACAGAAGGAGAGAATGAATCCAGAGGAG
TATGAGAGGCTTAAGGGGAGCTATATTATTACATACGAGTACCTCTCACGTTTCAAGAAGATACCCATGATAATGCATCC
ACTCCCAAGAGTGTGGGAACTCACCACCGATGTAGACGTGCTACCCCAAGCGATATACTTTGAACAAGCCAAGAACGGTC
TCTACATGAGGATGGCATTGATAAAAATAATCCTCGGAGTCTAA

Upstream 100 bases:

>100_bases
TATACCGTGATGGCGTGATCACAGATAAACCTGTTGGAAAATGGATTAGACCAGTTAAATTATAACGGGGGCTCACCATA
AAGTAGTTGTGGTGTACCCC

Downstream 100 bases:

>100_bases
TCGATGCAAATTTAAATCATGGATTTAAAGATAAATAGAGTTTTTAAGCCTCGGTATGCTGTTTCCGCTTTCATATAGGT
TTGCTATTAAATTCTTTTGT

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MYKGMDVISALQFSRSDVEKLITIAEELRRRVENGEKLELAKGRILFTAFFEPSTRTRLSFQFAMAKLGGNVIDLGPEEV
TSRAKGESPEDTLRTVDSYDPDIIVVRHREPGFAAKAAEICRAPVVNAGDGYNEHPTQALLDVYTIWRSLGRIDGVTIGI
MGDLKYGRTISSLSYILSNFRDVTIYFISPQALRPREEVLKILDQRRTRYELLEKPDDAMEKLNILYVTRLQKERMNPEE
YERLKGSYIITYEYLSRFKKIPMIMHPLPRVWELTTDVDVLPQAIYFEQAKNGLYMRMALIKIILGV

Sequences:

>Translated_307_residues
MYKGMDVISALQFSRSDVEKLITIAEELRRRVENGEKLELAKGRILFTAFFEPSTRTRLSFQFAMAKLGGNVIDLGPEEV
TSRAKGESPEDTLRTVDSYDPDIIVVRHREPGFAAKAAEICRAPVVNAGDGYNEHPTQALLDVYTIWRSLGRIDGVTIGI
MGDLKYGRTISSLSYILSNFRDVTIYFISPQALRPREEVLKILDQRRTRYELLEKPDDAMEKLNILYVTRLQKERMNPEE
YERLKGSYIITYEYLSRFKKIPMIMHPLPRVWELTTDVDVLPQAIYFEQAKNGLYMRMALIKIILGV
>Mature_307_residues
MYKGMDVISALQFSRSDVEKLITIAEELRRRVENGEKLELAKGRILFTAFFEPSTRTRLSFQFAMAKLGGNVIDLGPEEV
TSRAKGESPEDTLRTVDSYDPDIIVVRHREPGFAAKAAEICRAPVVNAGDGYNEHPTQALLDVYTIWRSLGRIDGVTIGI
MGDLKYGRTISSLSYILSNFRDVTIYFISPQALRPREEVLKILDQRRTRYELLEKPDDAMEKLNILYVTRLQKERMNPEE
YERLKGSYIITYEYLSRFKKIPMIMHPLPRVWELTTDVDVLPQAIYFEQAKNGLYMRMALIKIILGV

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=304, Percent_Identity=43.0921052631579, Blast_Score=263, Evalue=2e-70,
Organism=Homo sapiens, GI38788445, Length=314, Percent_Identity=25.796178343949, Blast_Score=76, Evalue=3e-14,
Organism=Escherichia coli, GI2367364, Length=302, Percent_Identity=40.7284768211921, Blast_Score=238, Evalue=5e-64,
Organism=Caenorhabditis elegans, GI193204318, Length=323, Percent_Identity=38.0804953560372, Blast_Score=228, Evalue=4e-60,
Organism=Saccharomyces cerevisiae, GI6322331, Length=311, Percent_Identity=44.0514469453376, Blast_Score=239, Evalue=4e-64,
Organism=Drosophila melanogaster, GI24642586, Length=306, Percent_Identity=39.2156862745098, Blast_Score=235, Evalue=2e-62,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 35324; Mature: 35324

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKGMDVISALQFSRSDVEKLITIAEELRRRVENGEKLELAKGRILFTAFFEPSTRTRLS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCEEEEEEEECCCCCHHHH
FQFAMAKLGGNVIDLGPEEVTSRAKGESPEDTLRTVDSYDPDIIVVRHREPGFAAKAAEI
HHHHHHHHCCCEEECCHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHH
CRAPVVNAGDGYNEHPTQALLDVYTIWRSLGRIDGVTIGIMGDLKYGRTISSLSYILSNF
HCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHCC
RDVTIYFISPQALRPREEVLKILDQRRTRYELLEKPDDAMEKLNILYVTRLQKERMNPEE
CEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEEHHHHHCCCHHH
YERLKGSYIITYEYLSRFKKIPMIMHPLPRVWELTTDVDVLPQAIYFEQAKNGLYMRMAL
HHHHCCCEEEEHHHHHHHHHCCHHHCCCCHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHH
IKIILGV
HHHHHCC
>Mature Secondary Structure
MYKGMDVISALQFSRSDVEKLITIAEELRRRVENGEKLELAKGRILFTAFFEPSTRTRLS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCEEEEEEEECCCCCHHHH
FQFAMAKLGGNVIDLGPEEVTSRAKGESPEDTLRTVDSYDPDIIVVRHREPGFAAKAAEI
HHHHHHHHCCCEEECCHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHH
CRAPVVNAGDGYNEHPTQALLDVYTIWRSLGRIDGVTIGIMGDLKYGRTISSLSYILSNF
HCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHCC
RDVTIYFISPQALRPREEVLKILDQRRTRYELLEKPDDAMEKLNILYVTRLQKERMNPEE
CEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEEHHHHHCCCHHH
YERLKGSYIITYEYLSRFKKIPMIMHPLPRVWELTTDVDVLPQAIYFEQAKNGLYMRMAL
HHHHCCCEEEEHHHHHHHHHCCHHHCCCCHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHH
IKIILGV
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11792869 [H]