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The map label for this gene is trpB

Identifier: 218884056

GI number: 218884056

Start: 714981

End: 716267

Strand: Direct

Name: trpB

Synonym: DKAM_0745

Alternate gene names: 218884056

Gene position: 714981-716267 (Clockwise)

Preceding gene: 218884053

Following gene: 218884057

Centisome position: 52.37

GC content: 47.16

Gene sequence:

>1287_bases
ATGGAATTAAGAAGTACCCAAACCCCCGATATACTCCCATCCTACTGGTATAACATAGTACCCGATCTACCTGAACCACT
ACCTCCCATGATTCATCCTAGCGGGGAGCCAGTTAAAAGAAGCGAGCTGGAAGTATTGTTCCCCAGGAGTCTCGTGGACC
AGGAGATCAGTACACAGAGCCGAATACCTATCCCCGAGGCTGTCAGAGAGACACTACTTATGCTGGGACGGCCCACGCCA
TTGATAAGGGCGAGAAGACTGGAAGAAGCTTTGAAGACTAATGCAAGAATATTCTTTAAATACGAGGGTGTACTCCCCAC
GGGATCCCATAAAATCAATACAGCTGTTGCGCAGGCATACTATAATGCAGTTGAAAGCGTTGAAAGGGTGTCCACTGAGA
CCGGCGCTGGTCAGTGGGGCTCAGCTCTCTCATTAGCTGGCAACATATACGGGTTAAAGGTAAGGGTATTCATGGTAAGG
GCTAGTTATCTTCAGAAACCTTATAGAAGGATCCTGATGGAGGCCTATGGTGCCGAAGTATATCCTAGTCCAAGCACTAA
GACCAATACCGGTAGAAAGCTCTTGGCAGAGAACCCTGATCACCCTGGCAGCCTAGGTATAGCTATAAGTGAAGCAATAG
AAGATGTGGTGTTGAGTGGGGGTAGAGCTAAGTATAGCCTTGGAAGCGTCTTAAACCATGTACTACTGCATCAATCTGTG
ATAGGGTTAGAAGCCATTAAGCAATTAGAGTTACTAGGAGTTGAGAGAGTAGACTATGTTATTGGATGCGTTGGAGGAGG
CTCCAACTTCGCTGGGCTCTCATACCCATTCATAGGCTACAACTATAATAATGGCGAGCCACCGGTGTTCATCGCTGTTG
AACCAGAAACAGTTCCCTCTATGACCAGGGGTGAGTATAGATACGACTACGGCGATACAGCCGGATTAACACCATTATTA
AAAATGTATACCCTGGGACACGACTACATCCCACCGCCAATATATGCAGGTGGATTAAGATACCATGGCGTTGCTCCCTC
GCTAAGCATACTTGTGAAAAACAATATCGTAAAACCAGTTGCATACCCTCAGGACGAGGTATTGGAGGCTGGACTCGTAT
TCGCCAGAACCCAGGGTATTCTTCCAGCCCCTGAAAGCTCTCATGCAGTGAAAGCAGTTATAGATGTAGCGTTGAAATAC
AGGGGTGCCAGCGAGAAACCAACAATATTATTCAATCTAAGCGGACACGGGCTACTGGATCTCTCAGCGTACACGAGGAA
TCATTAG

Upstream 100 bases:

>100_bases
TCTATATTTTATACATAATCTTCTCATTAATTATTATACAAAAGCAAAGATTTATATGTCAAACCCCCTAAATATTACCT
CCTGGTGATACTATGTTGGA

Downstream 100 bases:

>100_bases
TGGTATTGGATTAGAATTATGGCGAGCTCTATTTAAAGTAAGATATTTTGTATTTTTATTTAATCTCCAATCCAGTAAAT
CATGGGAAATATAATGTTTA

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MELRSTQTPDILPSYWYNIVPDLPEPLPPMIHPSGEPVKRSELEVLFPRSLVDQEISTQSRIPIPEAVRETLLMLGRPTP
LIRARRLEEALKTNARIFFKYEGVLPTGSHKINTAVAQAYYNAVESVERVSTETGAGQWGSALSLAGNIYGLKVRVFMVR
ASYLQKPYRRILMEAYGAEVYPSPSTKTNTGRKLLAENPDHPGSLGIAISEAIEDVVLSGGRAKYSLGSVLNHVLLHQSV
IGLEAIKQLELLGVERVDYVIGCVGGGSNFAGLSYPFIGYNYNNGEPPVFIAVEPETVPSMTRGEYRYDYGDTAGLTPLL
KMYTLGHDYIPPPIYAGGLRYHGVAPSLSILVKNNIVKPVAYPQDEVLEAGLVFARTQGILPAPESSHAVKAVIDVALKY
RGASEKPTILFNLSGHGLLDLSAYTRNH

Sequences:

>Translated_428_residues
MELRSTQTPDILPSYWYNIVPDLPEPLPPMIHPSGEPVKRSELEVLFPRSLVDQEISTQSRIPIPEAVRETLLMLGRPTP
LIRARRLEEALKTNARIFFKYEGVLPTGSHKINTAVAQAYYNAVESVERVSTETGAGQWGSALSLAGNIYGLKVRVFMVR
ASYLQKPYRRILMEAYGAEVYPSPSTKTNTGRKLLAENPDHPGSLGIAISEAIEDVVLSGGRAKYSLGSVLNHVLLHQSV
IGLEAIKQLELLGVERVDYVIGCVGGGSNFAGLSYPFIGYNYNNGEPPVFIAVEPETVPSMTRGEYRYDYGDTAGLTPLL
KMYTLGHDYIPPPIYAGGLRYHGVAPSLSILVKNNIVKPVAYPQDEVLEAGLVFARTQGILPAPESSHAVKAVIDVALKY
RGASEKPTILFNLSGHGLLDLSAYTRNH
>Mature_428_residues
MELRSTQTPDILPSYWYNIVPDLPEPLPPMIHPSGEPVKRSELEVLFPRSLVDQEISTQSRIPIPEAVRETLLMLGRPTP
LIRARRLEEALKTNARIFFKYEGVLPTGSHKINTAVAQAYYNAVESVERVSTETGAGQWGSALSLAGNIYGLKVRVFMVR
ASYLQKPYRRILMEAYGAEVYPSPSTKTNTGRKLLAENPDHPGSLGIAISEAIEDVVLSGGRAKYSLGSVLNHVLLHQSV
IGLEAIKQLELLGVERVDYVIGCVGGGSNFAGLSYPFIGYNYNNGEPPVFIAVEPETVPSMTRGEYRYDYGDTAGLTPLL
KMYTLGHDYIPPPIYAGGLRYHGVAPSLSILVKNNIVKPVAYPQDEVLEAGLVFARTQGILPAPESSHAVKAVIDVALKY
RGASEKPTILFNLSGHGLLDLSAYTRNH

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine

COG id: COG1350

COG function: function code R; Predicted alternative tryptophan synthase beta-subunit (paralog of TrpB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family

Homologues:

Organism=Escherichia coli, GI1787515, Length=402, Percent_Identity=29.8507462686567, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6321412, Length=362, Percent_Identity=29.8342541436464, Blast_Score=104, Evalue=2e-23,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPB_DESK1 (B8D4P0)

Other databases:

- EMBL:   CP001140
- RefSeq:   YP_002428438.1
- GeneID:   7170912
- GenomeReviews:   CP001140_GR
- KEGG:   dka:DKAM_0745
- HOGENOM:   HBG303148
- OMA:   YTLGHDF
- ProtClustDB:   PRK12391
- HAMAP:   MF_00133
- InterPro:   IPR001926
- InterPro:   IPR006316
- InterPro:   IPR006653
- InterPro:   IPR023026
- TIGRFAMs:   TIGR01415

Pfam domain/function: PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =4.2.1.20

Molecular weight: Translated: 46870; Mature: 46870

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRSTQTPDILPSYWYNIVPDLPEPLPPMIHPSGEPVKRSELEVLFPRSLVDQEISTQS
CCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHCCCC
RIPIPEAVRETLLMLGRPTPLIRARRLEEALKTNARIFFKYEGVLPTGSHKINTAVAQAY
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHH
YNAVESVERVSTETGAGQWGSALSLAGNIYGLKVRVFMVRASYLQKPYRRILMEAYGAEV
HHHHHHHHHHHHCCCCCCCCCHHHHCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHCCCC
YPSPSTKTNTGRKLLAENPDHPGSLGIAISEAIEDVVLSGGRAKYSLGSVLNHVLLHQSV
CCCCCCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
IGLEAIKQLELLGVERVDYVIGCVGGGSNFAGLSYPFIGYNYNNGEPPVFIAVEPETVPS
HHHHHHHHHHHHCHHHEEEEEEEECCCCCCCCCCCCEEEEECCCCCCCEEEEECCCCCCC
MTRGEYRYDYGDTAGLTPLLKMYTLGHDYIPPPIYAGGLRYHGVAPSLSILVKNNIVKPV
CCCCCEEECCCCCCCHHHHHHHHHCCCCCCCCCCEECCEEECCCCCCEEEEEECCCCCCC
AYPQDEVLEAGLVFARTQGILPAPESSHAVKAVIDVALKYRGASEKPTILFNLSGHGLLD
CCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEE
LSAYTRNH
ECHHHCCC
>Mature Secondary Structure
MELRSTQTPDILPSYWYNIVPDLPEPLPPMIHPSGEPVKRSELEVLFPRSLVDQEISTQS
CCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHCCCC
RIPIPEAVRETLLMLGRPTPLIRARRLEEALKTNARIFFKYEGVLPTGSHKINTAVAQAY
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHH
YNAVESVERVSTETGAGQWGSALSLAGNIYGLKVRVFMVRASYLQKPYRRILMEAYGAEV
HHHHHHHHHHHHCCCCCCCCCHHHHCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHCCCC
YPSPSTKTNTGRKLLAENPDHPGSLGIAISEAIEDVVLSGGRAKYSLGSVLNHVLLHQSV
CCCCCCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
IGLEAIKQLELLGVERVDYVIGCVGGGSNFAGLSYPFIGYNYNNGEPPVFIAVEPETVPS
HHHHHHHHHHHHCHHHEEEEEEEECCCCCCCCCCCCEEEEECCCCCCCEEEEECCCCCCC
MTRGEYRYDYGDTAGLTPLLKMYTLGHDYIPPPIYAGGLRYHGVAPSLSILVKNNIVKPV
CCCCCEEECCCCCCCHHHHHHHHHCCCCCCCCCCEECCEEECCCCCCEEEEEECCCCCCC
AYPQDEVLEAGLVFARTQGILPAPESSHAVKAVIDVALKYRGASEKPTILFNLSGHGLLD
CCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEE
LSAYTRNH
ECHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA