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The map label for this gene is fabH [C]

Identifier: 218883701

GI number: 218883701

Start: 360817

End: 361875

Strand: Reverse

Name: fabH [C]

Synonym: DKAM_0390

Alternate gene names: 218883701

Gene position: 361875-360817 (Counterclockwise)

Preceding gene: 218883702

Following gene: 218883700

Centisome position: 26.51

GC content: 48.91

Gene sequence:

>1059_bases
ATGATTCCTGAGGAACGGGTAGGGATAATTGGCTGGGGCTCATATATACCTAGGTGGAGACTACCCCTCAGCGAAATAGT
TAGGACGTGGGGATTTAATCCTAAGGAACCAGAAGAATTAAATGTCGCCGAGAAGAGTGTCGCCGGGATAGATGAAGATG
CCATCACAATGGGCTGGGAGGCCGCTGTAAACGCATTGAGACGTGCTAGAATAGACCCGAGGAAAATCGGGGCAGTATGG
TTTGGGACTGAGAGTAAGCCCTATGCTGTAAAACCCTCGGCAACGATTATAGCTGAGGCTCTAGGTATAGCTCCTGAAAC
CATGGCGACAGACCTGGAGTTCGCGTGTAGAGCTGCAAGTGAGGCGCTGAGGATAAGTATTGGAGCTGTGGCATCTGGTT
TGATAAACTATGCGCTAGTAATAGGTAGTGATACAGCACAAGCAAACCCAGGGGACGTGTTAGAATTCACAGCTTCCTCA
GCTGCTACAGCCCTTATAACGGGGCCTAGGGAAGAGTCAGCGGCATACTTCGAGGCAAGTGCCACATTCGTCACCGATAC
ACCTGATTTCTGGCGTAGGGATCATTCGCGCTACCCACTTCATGGTGAGGCATTCACAGGTGAGCCAGCATACTTTAAAC
ATATAGTGGGCGCTGTGAAACTACTAATGGAGAAGACCGGGCTTAAACCAGAGGACTTCGATTACGCTATCTTCCACCAG
CCAAACGGTACATTCCCGCTTAGGGTTGCAAGAATGCTTGGAATACCCAAGGAGAAAGTAATACCTGGATTAGTCACACC
GTTCATAGGTAACAGCTATAATTCGTCAGCCCTCATAGGGCTTTCCCGCGTGCTTGATAAGGCTAAGCCGGGCCAGAGGA
TTCTCTTGGCACCATTCGGCAGTGGTGCTGGTAGTGACGCCTACAGTATCATTGTAACTGATAGGATCATGGAGAAACAG
GAATTAGCACCCACCGTGGATGACTATATAGTGAGGAAGGCATTGATAGATTATGCCCTATATGCTAAATACAGGAGGCT
GATTAACAGGATAAGGTGA

Upstream 100 bases:

>100_bases
CCGTTGGTCGAGGTTGAGCCAGGCCACTACGTCAAGTGCTGGCTATACGCTAAAAAATGATTTTTATCGAGGTAATAAAA
TAGTGTAGAGGGATGCCTTT

Downstream 100 bases:

>100_bases
AAAATGATGAAGATATCTGTACCTCCATCCTGGAGAACAAGACTTGACAGATACAGGCTTAAAGCCAGTAAGTGCAGGGA
CTGTGGTAGAGTGGCCTATC

Product: hypothetical protein

Products: 3-oxoacyl-[acyl-carrier protein]; CO2; [acyl-carrier protein]

Alternate protein names: NA

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MIPEERVGIIGWGSYIPRWRLPLSEIVRTWGFNPKEPEELNVAEKSVAGIDEDAITMGWEAAVNALRRARIDPRKIGAVW
FGTESKPYAVKPSATIIAEALGIAPETMATDLEFACRAASEALRISIGAVASGLINYALVIGSDTAQANPGDVLEFTASS
AATALITGPREESAAYFEASATFVTDTPDFWRRDHSRYPLHGEAFTGEPAYFKHIVGAVKLLMEKTGLKPEDFDYAIFHQ
PNGTFPLRVARMLGIPKEKVIPGLVTPFIGNSYNSSALIGLSRVLDKAKPGQRILLAPFGSGAGSDAYSIIVTDRIMEKQ
ELAPTVDDYIVRKALIDYALYAKYRRLINRIR

Sequences:

>Translated_352_residues
MIPEERVGIIGWGSYIPRWRLPLSEIVRTWGFNPKEPEELNVAEKSVAGIDEDAITMGWEAAVNALRRARIDPRKIGAVW
FGTESKPYAVKPSATIIAEALGIAPETMATDLEFACRAASEALRISIGAVASGLINYALVIGSDTAQANPGDVLEFTASS
AATALITGPREESAAYFEASATFVTDTPDFWRRDHSRYPLHGEAFTGEPAYFKHIVGAVKLLMEKTGLKPEDFDYAIFHQ
PNGTFPLRVARMLGIPKEKVIPGLVTPFIGNSYNSSALIGLSRVLDKAKPGQRILLAPFGSGAGSDAYSIIVTDRIMEKQ
ELAPTVDDYIVRKALIDYALYAKYRRLINRIR
>Mature_352_residues
MIPEERVGIIGWGSYIPRWRLPLSEIVRTWGFNPKEPEELNVAEKSVAGIDEDAITMGWEAAVNALRRARIDPRKIGAVW
FGTESKPYAVKPSATIIAEALGIAPETMATDLEFACRAASEALRISIGAVASGLINYALVIGSDTAQANPGDVLEFTASS
AATALITGPREESAAYFEASATFVTDTPDFWRRDHSRYPLHGEAFTGEPAYFKHIVGAVKLLMEKTGLKPEDFDYAIFHQ
PNGTFPLRVARMLGIPKEKVIPGLVTPFIGNSYNSSALIGLSRVLDKAKPGQRILLAPFGSGAGSDAYSIIVTDRIMEKQ
ELAPTVDDYIVRKALIDYALYAKYRRLINRIR

Specific function: Catalyzes The Condensation Reaction Of Fatty Acid Synthesis By The Addition To An Acyl Acceptor Of Two Carbons From Malonyl-Acp. Catalyzes The First Condensation Reaction Which Initiates Fatty Acid Synthesis And May Therefore Play A Role In Governing The

COG id: COG3425

COG function: function code I; 3-hydroxy-3-methylglutaryl CoA synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0219 family [H]

Homologues:

Organism=Escherichia coli, GI1787333, Length=315, Percent_Identity=27.6190476190476, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013747
- InterPro:   IPR004656
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF08541 ACP_syn_III_C [H]

EC number: 2.3.1.41

Molecular weight: Translated: 38548; Mature: 38548

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: PS00678 WD_REPEATS_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPEERVGIIGWGSYIPRWRLPLSEIVRTWGFNPKEPEELNVAEKSVAGIDEDAITMGWE
CCCCCCEEEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCHHHCCHH
AAVNALRRARIDPRKIGAVWFGTESKPYAVKPSATIIAEALGIAPETMATDLEFACRAAS
HHHHHHHHHCCCHHHEEEEEECCCCCCEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHH
EALRISIGAVASGLINYALVIGSDTAQANPGDVLEFTASSAATALITGPREESAAYFEAS
HHHEEHHHHHHHHHHHEEEEEECCCCCCCCCCEEEEECCCCCEEEEECCCCCCCEEEEEC
ATFVTDTPDFWRRDHSRYPLHGEAFTGEPAYFKHIVGAVKLLMEKTGLKPEDFDYAIFHQ
EEEEECCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEC
PNGTFPLRVARMLGIPKEKVIPGLVTPFIGNSYNSSALIGLSRVLDKAKPGQRILLAPFG
CCCCCHHHHHHHHCCCHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECC
SGAGSDAYSIIVTDRIMEKQELAPTVDDYIVRKALIDYALYAKYRRLINRIR
CCCCCCEEEEEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIPEERVGIIGWGSYIPRWRLPLSEIVRTWGFNPKEPEELNVAEKSVAGIDEDAITMGWE
CCCCCCEEEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCHHHCCHH
AAVNALRRARIDPRKIGAVWFGTESKPYAVKPSATIIAEALGIAPETMATDLEFACRAAS
HHHHHHHHHCCCHHHEEEEEECCCCCCEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHH
EALRISIGAVASGLINYALVIGSDTAQANPGDVLEFTASSAATALITGPREESAAYFEAS
HHHEEHHHHHHHHHHHEEEEEECCCCCCCCCCEEEEECCCCCEEEEECCCCCCCEEEEEC
ATFVTDTPDFWRRDHSRYPLHGEAFTGEPAYFKHIVGAVKLLMEKTGLKPEDFDYAIFHQ
EEEEECCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEC
PNGTFPLRVARMLGIPKEKVIPGLVTPFIGNSYNSSALIGLSRVLDKAKPGQRILLAPFG
CCCCCHHHHHHHHCCCHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECC
SGAGSDAYSIIVTDRIMEKQELAPTVDDYIVRKALIDYALYAKYRRLINRIR
CCCCCCEEEEEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 2-Mercaptoethanol; DTT; EDTA [C]

Metal ions: Phosphate [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.138 {cis-9-hexadecenoyl-ACP}} 0.017 {cis-9-hexadecenoyl-ACP}} 0.012 {cis-3-decenoyl-ACP}} 0.04 {acetyl-ACP}} 0.031 {acetyl-ACP}} [C]

Substrates: acyl-[acyl-carrier protein]; malonyl-[acyl-carrier protein]

Specific reaction: acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] = 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]

General reaction: Acyl group transfer [C]

Inhibitor: Acetyl-CoA; Acyl-CoA; Iodoacetamide; N-Ethyl maleimide acyl -ACP-derivatives protect, malonyl -ACP-decarboxyl ation, [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]