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The map label for this gene is glyA [H]

Identifier: 218710337

GI number: 218710337

Start: 2539700

End: 2540950

Strand: Reverse

Name: glyA [H]

Synonym: VS_2373

Alternate gene names: 218710337

Gene position: 2540950-2539700 (Counterclockwise)

Preceding gene: 218710341

Following gene: 218710330

Centisome position: 77.01

GC content: 46.28

Gene sequence:

>1251_bases
ATGCTTAAGCGTGACATGAACATTGCTGATTACGATGCGGACCTATTCGCAGCTATCCAAGAAGAAACTCTTCGTCAGGA
AGAGCACATCGAACTTATCGCTTCAGAAAACTACACAAGCCCACGTGTAATGGAAGCTCAAGGTTCTCAACTTACAAACA
AATACGCTGAAGGCTACCCTGGTAAGCGTTACTACGGTGGTTGTGAGTTCGTAGATAAAGTTGAAACTCTAGCAATCGAA
CGTGCATGTGAACTGTTTGGCGCTCAATACGCGAACGTACAACCTCACTCAGGTTCTCAAGCAAACAACGCTGTATACAT
GGCACTACTGAATGCTGGCGATACTGTTCTAGGTATGAGCCTAGCGCACGGTGGTCACCTAACTCACGGTTCTCCAGTAA
ACTTCTCTGGTAAGCTTTACAACATCATCCCTTACGGTATCGATGAAGCAGGTCAAATCGATTACGAAGAGATGGAAGCG
CTAGCTATCGAGCACAAACCTAAGATGATCATCGGTGGTTTCTCTGCTTACTCTCAAATTTGTGATTGGGCGCGCATGCG
TGAAATCGCTGACAAAGTAGGTGCTTACTTCTTCGTAGATATGGCTCACGTTGCGGGTCTAATCGCTGCAGGCGTTTACC
CGAACCCAGTTCCACACGCTCACGTTGTTACAACAACAACGCACAAAACGCTTGCTGGTCCTCGTGGTGGTCTTATCCTT
TCTAACGAAGGTGAAGATCTTTACAAGAAGCTAAACTCAGCAGTATTCCCAGGCGGCCAAGGCGGTCCTCTAATGCACGT
TATCGCTGGTAAAGCAGTAGCGTTCAAAGAAGCTCTAGAACCAGAGTTCAAAGAATACCAAGCTCGCGTTGTTGCTAACG
CAAAAGCAATGGTTGCTGAATTCCTAGCACGCGGTTACAACATCGTTTCAGGTTCTACAGAGAACCACCTGTTCCTAGTT
GACCTAATCGACAAAGACATCACAGGTAAAGAGGCTGATGCAGCACTAGGTTCAGCTAACATCACAGTTAACAAGAACTC
AGTTCCAAACGATCCACGTAGCCCATTCGTTACTTCTGGTATCCGTATCGGTTCTCCTTCTATCACTCGTCGCGGTTTCT
CAGAAGCAGACGCGAAAGAGCTAGCAGGTTGGATGTGTGACATCCTAGATAACATGGGTGATGAGTCTGTTATCGAAGCA
ACTAAAGCGAAAGTACTAAATATCTGTAAGCGTCTACCTGTTTACGCATAA

Upstream 100 bases:

>100_bases
TGTAGAATGCGTCACATCAAAACGGTGACGGAAACGTTTGCTTTCGGTCGTTGTGTGGTTTTTTTGAAACTTTCAGTACA
ATCTGAGTCAGGAGATACAG

Downstream 100 bases:

>100_bases
TTTTTCTGCGTAGTAATTTGAACTTCGTTGTCGAGCATGCTCACTTACATCCGTAAGCTCCGCGTGCTCTCCTAGAATTT
CAAACGACTACTTTGAAAAG

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT 1; Serine methylase 1 [H]

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MLKRDMNIADYDADLFAAIQEETLRQEEHIELIASENYTSPRVMEAQGSQLTNKYAEGYPGKRYYGGCEFVDKVETLAIE
RACELFGAQYANVQPHSGSQANNAVYMALLNAGDTVLGMSLAHGGHLTHGSPVNFSGKLYNIIPYGIDEAGQIDYEEMEA
LAIEHKPKMIIGGFSAYSQICDWARMREIADKVGAYFFVDMAHVAGLIAAGVYPNPVPHAHVVTTTTHKTLAGPRGGLIL
SNEGEDLYKKLNSAVFPGGQGGPLMHVIAGKAVAFKEALEPEFKEYQARVVANAKAMVAEFLARGYNIVSGSTENHLFLV
DLIDKDITGKEADAALGSANITVNKNSVPNDPRSPFVTSGIRIGSPSITRRGFSEADAKELAGWMCDILDNMGDESVIEA
TKAKVLNICKRLPVYA

Sequences:

>Translated_416_residues
MLKRDMNIADYDADLFAAIQEETLRQEEHIELIASENYTSPRVMEAQGSQLTNKYAEGYPGKRYYGGCEFVDKVETLAIE
RACELFGAQYANVQPHSGSQANNAVYMALLNAGDTVLGMSLAHGGHLTHGSPVNFSGKLYNIIPYGIDEAGQIDYEEMEA
LAIEHKPKMIIGGFSAYSQICDWARMREIADKVGAYFFVDMAHVAGLIAAGVYPNPVPHAHVVTTTTHKTLAGPRGGLIL
SNEGEDLYKKLNSAVFPGGQGGPLMHVIAGKAVAFKEALEPEFKEYQARVVANAKAMVAEFLARGYNIVSGSTENHLFLV
DLIDKDITGKEADAALGSANITVNKNSVPNDPRSPFVTSGIRIGSPSITRRGFSEADAKELAGWMCDILDNMGDESVIEA
TKAKVLNICKRLPVYA
>Mature_416_residues
MLKRDMNIADYDADLFAAIQEETLRQEEHIELIASENYTSPRVMEAQGSQLTNKYAEGYPGKRYYGGCEFVDKVETLAIE
RACELFGAQYANVQPHSGSQANNAVYMALLNAGDTVLGMSLAHGGHLTHGSPVNFSGKLYNIIPYGIDEAGQIDYEEMEA
LAIEHKPKMIIGGFSAYSQICDWARMREIADKVGAYFFVDMAHVAGLIAAGVYPNPVPHAHVVTTTTHKTLAGPRGGLIL
SNEGEDLYKKLNSAVFPGGQGGPLMHVIAGKAVAFKEALEPEFKEYQARVVANAKAMVAEFLARGYNIVSGSTENHLFLV
DLIDKDITGKEADAALGSANITVNKNSVPNDPRSPFVTSGIRIGSPSITRRGFSEADAKELAGWMCDILDNMGDESVIEA
TKAKVLNICKRLPVYA

Specific function: Interconversion of serine and glycine [H]

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family [H]

Homologues:

Organism=Homo sapiens, GI261862352, Length=402, Percent_Identity=45.5223880597015, Blast_Score=344, Evalue=1e-94,
Organism=Homo sapiens, GI261862350, Length=402, Percent_Identity=45.5223880597015, Blast_Score=344, Evalue=1e-94,
Organism=Homo sapiens, GI261862348, Length=402, Percent_Identity=45.5223880597015, Blast_Score=344, Evalue=1e-94,
Organism=Homo sapiens, GI19923315, Length=402, Percent_Identity=45.5223880597015, Blast_Score=343, Evalue=1e-94,
Organism=Homo sapiens, GI261862346, Length=401, Percent_Identity=44.6384039900249, Blast_Score=332, Evalue=4e-91,
Organism=Homo sapiens, GI22547186, Length=401, Percent_Identity=46.1346633416459, Blast_Score=318, Evalue=7e-87,
Organism=Homo sapiens, GI22547189, Length=388, Percent_Identity=43.5567010309278, Blast_Score=286, Evalue=3e-77,
Organism=Escherichia coli, GI1788902, Length=417, Percent_Identity=81.0551558752998, Blast_Score=690, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25144732, Length=401, Percent_Identity=44.6384039900249, Blast_Score=337, Evalue=5e-93,
Organism=Caenorhabditis elegans, GI25144729, Length=401, Percent_Identity=44.6384039900249, Blast_Score=337, Evalue=7e-93,
Organism=Saccharomyces cerevisiae, GI6319739, Length=401, Percent_Identity=44.8877805486284, Blast_Score=360, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6323087, Length=402, Percent_Identity=44.2786069651741, Blast_Score=337, Evalue=3e-93,
Organism=Drosophila melanogaster, GI24640005, Length=400, Percent_Identity=45.25, Blast_Score=348, Evalue=3e-96,
Organism=Drosophila melanogaster, GI221329721, Length=400, Percent_Identity=45.25, Blast_Score=347, Evalue=6e-96,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798 [H]

Pfam domain/function: PF00464 SHMT [H]

EC number: =2.1.2.1 [H]

Molecular weight: Translated: 45087; Mature: 45087

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKRDMNIADYDADLFAAIQEETLRQEEHIELIASENYTSPRVMEAQGSQLTNKYAEGYP
CCCCCCCCCCCCHHHHHHHHHHHHCHHHHEEEEECCCCCCCEEECCCCHHHHHHHHCCCC
GKRYYGGCEFVDKVETLAIERACELFGAQYANVQPHSGSQANNAVYMALLNAGDTVLGMS
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEE
LAHGGHLTHGSPVNFSGKLYNIIPYGIDEAGQIDYEEMEALAIEHKPKMIIGGFSAYSQI
ECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCEEEECHHHHHHH
CDWARMREIADKVGAYFFVDMAHVAGLIAAGVYPNPVPHAHVVTTTTHKTLAGPRGGLIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHCCCCCCEEE
SNEGEDLYKKLNSAVFPGGQGGPLMHVIAGKAVAFKEALEPEFKEYQARVVANAKAMVAE
ECCCHHHHHHHHHCCCCCCCCCCEEEEHHCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
FLARGYNIVSGSTENHLFLVDLIDKDITGKEADAALGSANITVNKNSVPNDPRSPFVTSG
HHHCCCEEECCCCCCCEEEEEECCCCCCCCCHHHHCCCCEEEECCCCCCCCCCCCCEECC
IRIGSPSITRRGFSEADAKELAGWMCDILDNMGDESVIEATKAKVLNICKRLPVYA
EEECCCCHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLKRDMNIADYDADLFAAIQEETLRQEEHIELIASENYTSPRVMEAQGSQLTNKYAEGYP
CCCCCCCCCCCCHHHHHHHHHHHHCHHHHEEEEECCCCCCCEEECCCCHHHHHHHHCCCC
GKRYYGGCEFVDKVETLAIERACELFGAQYANVQPHSGSQANNAVYMALLNAGDTVLGMS
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEE
LAHGGHLTHGSPVNFSGKLYNIIPYGIDEAGQIDYEEMEALAIEHKPKMIIGGFSAYSQI
ECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCEEEECHHHHHHH
CDWARMREIADKVGAYFFVDMAHVAGLIAAGVYPNPVPHAHVVTTTTHKTLAGPRGGLIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHCCCCCCEEE
SNEGEDLYKKLNSAVFPGGQGGPLMHVIAGKAVAFKEALEPEFKEYQARVVANAKAMVAE
ECCCHHHHHHHHHCCCCCCCCCCEEEEHHCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
FLARGYNIVSGSTENHLFLVDLIDKDITGKEADAALGSANITVNKNSVPNDPRSPFVTSG
HHHCCCEEECCCCCCCEEEEEECCCCCCCCCHHHHCCCCEEEECCCCCCCCCCCCCEECC
IRIGSPSITRRGFSEADAKELAGWMCDILDNMGDESVIEATKAKVLNICKRLPVYA
EEECCCCHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA