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The map label for this gene is metE [H]

Identifier: 218709138

GI number: 218709138

Start: 1213110

End: 1215458

Strand: Direct

Name: metE [H]

Synonym: VS_1145

Alternate gene names: 218709138

Gene position: 1213110-1215458 (Clockwise)

Preceding gene: 218709136

Following gene: 218709145

Centisome position: 36.77

GC content: 46.74

Gene sequence:

>2349_bases
ATGACGACAACAACGCATATTCTTGGCTACCCACGTATCGGTGAAAAACGCGAACTCAAATTCACTCTCGAGAAGTACTG
GCGCGGTGAGATCGATCAATCTGAGCTCAAGCAGACCGGCAGTGAATTAAGAAATCGTAACTGGAATGTACAAGCTGACG
CGAATCTAAGCTTTGCAACTGCGGGTGACTTCGCATGGTATGACCATGTACTAACTACGACTCTACTTCTAGGCCACGTT
CCCAAGCGCCATGCTGGTGGATCAGAAGATGAAAAAGCCTTCCCAGATTTAGATACCTTGTTCCGCGTTGGTCGTGGTCA
GTCTCAAGTTCAATCTACTTGCTGCGGTGGTTCGCATACGTCGAATGATGTCACTAAAGACAGTTCAGCTGCTTCTGACA
TGACCAAGTGGTTCAACACTAACTATCACTACATTGTTCCTGAGTTCAGCAAAGATGACTCTTTTGAAGTAAGTTGGCCT
CAACTGTTTGATGAAGTGAATGAAGCGATTAAAGCAGGACACAAAGTTAAGCCTGTACTCCTTGGGCCACTCTCTTACTT
ATACCTAGGCAAAGAAGTGGAAGATGGGTTTGACCGCTTATCTCTACTTCCTCGCCTTCTTACCGCGTATCAAGCCATTT
TGGCAAAGCTCTCAAAGCTTGGCGTAGAGTGGGTCCAAATTGATGAGCCAATCCTGGCTTTGGAGCTTGATAATCAGTGG
GTAGATTCATTCAAGCTGGCTTACCAAGTGATTCAAGGCGATGTAAAACTACTACTTACCACTTACTTTGATTCGGTCAC
GGATACATTAGATAAAATCGTAGATCTGCCTATCAACGGCTTACATATCGACTTAGCAGCAGCACCGCAGCAACTCGATG
AAGTGGTTAATAAACTACCGGAAGATTGGGTACTTTCTGCGGGTGCAATCAATGGACGCAATGTGTGGCGAGCTGACTTA
GCCGCGCAGCTTGAGTTACTGCAACCTGTGAAAGACAAACTAGGAGATAAGCTTTGGATCGCAAGTTCATGTTCATTGCT
GCATAGTCCTGTTGATTTGGAGTTAGAAGAAACGCTTAGCGAAGAAGTGAAGAGTTGGTTTGCCTTTGCAAAACAGAAAG
TGACCGAGGTGAGCTTGTTGGGTGCGGCGTTAGACGGTGATCAAAATGCCATTTTGGCGTGTGAGACTTACAGCCAACCA
ATTGTTGCCCGTAAGAGCGCGACTCATGTGAACAAGCCACAAGTTCAGGCTCGTATCAATACCATTACCAAAGCACTGGC
AGAACGCAGTGCACCATACGCAGAGCGCGCAGCGCATCAGTCTGAAGTTCTCGGCTTACCGCTGTTACCAACCACAACCA
TTGGTTCGTTCCCACAGACAGGAGAGATTCGTCTTCAGCGTAGTGCTTACCGAACTGGCAAACTGAATGAAGCGGAATAC
ACAACCGCATTGAAAGGTCATATTGCTGATGCGGTTAAGCGCCAAGAAGCCTTAGATTTGGATGTGCTTGTGCATGGCGA
GGCGGAACGCAATGACATGGTGGAGTACTTTGCAGAAAACCTAGCAGGCTTCCAAACCACTAAGTTTGGTTGGGTACAAA
GTTATGGCTCTCGTTGCGTGAAACCGGCGATTGTGGTTGCGGATATTGAGCGTGAAAAACCGATGACGGTCGAGTGGTCG
ACTTATGCTCAATCTCTGACATCTAAGCAGATGAAAGGCATGTTGACTGGGCCTGTGACAATTCTGTGTTGGACGTTCCC
GCGTGAAGATATCTCGCGTAAAGAGATCACTAACCAGCTGGCATTTGCTTTGCGTGATGAGGTGTCTGATTTACAAGACG
CTGGCATCAACATTATTCAAATCGATGAGCCTGCTATCCGTGAAGGTTTGCCTTTGAAAAAGCGCGACCATGCAGAGTAC
TTAGAATGGGCAGTGGATGCCTTTAAGATTTCAGCGGCGAGTGCTAAACCAGAAACTCAGATTCATACCCACATGTGTTA
CAGCGAGTTCAACGAGATCATTGATTCAGTGGCCGCGCTAGATGCCGATGTGATTACCATTGAGACATCTCGCTCGAACA
TGGAATTGCTGAAAGCGTTTGAAGAGTTTAACTATCCGAATGCGATTGGACCTGGCGTTTATGATATTCACTCGCCAAAC
ATCCCTTCAGAAGAGTGGATTGTAGATTTACTTAAGAAAGCGGCAGAAAAAATACCTGCAGAACGTTTGTGGGCAAACCC
TGATTGCGGATTAAAGACTCGTAACTGGGCAGAGACAGAAGCGGCACTTACTAACTTAGTTTCGGCGACGAAGACACTGC
GTAAAGAGTGGGAATCAGCTGAGGCATAA

Upstream 100 bases:

>100_bases
AGGGAGATAGTTTAGCCATCCAGACGCCTCGGTTGAAAGTTAAAACTACGGACTAAACTGTCTGACCGGAAATTAAAATT
ATCGAATAAGGAACAGACAC

Downstream 100 bases:

>100_bases
TTCAGTCTAAGATTTGATTGACCCTCTACTCCACGATGAACCACAAAAAAGGCCTCGCATTTGCGAGGCCTTCTGGTTTT
ATACTTGTTCGAATCTCGAA

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme [H]

Number of amino acids: Translated: 782; Mature: 781

Protein sequence:

>782_residues
MTTTTHILGYPRIGEKRELKFTLEKYWRGEIDQSELKQTGSELRNRNWNVQADANLSFATAGDFAWYDHVLTTTLLLGHV
PKRHAGGSEDEKAFPDLDTLFRVGRGQSQVQSTCCGGSHTSNDVTKDSSAASDMTKWFNTNYHYIVPEFSKDDSFEVSWP
QLFDEVNEAIKAGHKVKPVLLGPLSYLYLGKEVEDGFDRLSLLPRLLTAYQAILAKLSKLGVEWVQIDEPILALELDNQW
VDSFKLAYQVIQGDVKLLLTTYFDSVTDTLDKIVDLPINGLHIDLAAAPQQLDEVVNKLPEDWVLSAGAINGRNVWRADL
AAQLELLQPVKDKLGDKLWIASSCSLLHSPVDLELEETLSEEVKSWFAFAKQKVTEVSLLGAALDGDQNAILACETYSQP
IVARKSATHVNKPQVQARINTITKALAERSAPYAERAAHQSEVLGLPLLPTTTIGSFPQTGEIRLQRSAYRTGKLNEAEY
TTALKGHIADAVKRQEALDLDVLVHGEAERNDMVEYFAENLAGFQTTKFGWVQSYGSRCVKPAIVVADIEREKPMTVEWS
TYAQSLTSKQMKGMLTGPVTILCWTFPREDISRKEITNQLAFALRDEVSDLQDAGINIIQIDEPAIREGLPLKKRDHAEY
LEWAVDAFKISAASAKPETQIHTHMCYSEFNEIIDSVAALDADVITIETSRSNMELLKAFEEFNYPNAIGPGVYDIHSPN
IPSEEWIVDLLKKAAEKIPAERLWANPDCGLKTRNWAETEAALTNLVSATKTLRKEWESAEA

Sequences:

>Translated_782_residues
MTTTTHILGYPRIGEKRELKFTLEKYWRGEIDQSELKQTGSELRNRNWNVQADANLSFATAGDFAWYDHVLTTTLLLGHV
PKRHAGGSEDEKAFPDLDTLFRVGRGQSQVQSTCCGGSHTSNDVTKDSSAASDMTKWFNTNYHYIVPEFSKDDSFEVSWP
QLFDEVNEAIKAGHKVKPVLLGPLSYLYLGKEVEDGFDRLSLLPRLLTAYQAILAKLSKLGVEWVQIDEPILALELDNQW
VDSFKLAYQVIQGDVKLLLTTYFDSVTDTLDKIVDLPINGLHIDLAAAPQQLDEVVNKLPEDWVLSAGAINGRNVWRADL
AAQLELLQPVKDKLGDKLWIASSCSLLHSPVDLELEETLSEEVKSWFAFAKQKVTEVSLLGAALDGDQNAILACETYSQP
IVARKSATHVNKPQVQARINTITKALAERSAPYAERAAHQSEVLGLPLLPTTTIGSFPQTGEIRLQRSAYRTGKLNEAEY
TTALKGHIADAVKRQEALDLDVLVHGEAERNDMVEYFAENLAGFQTTKFGWVQSYGSRCVKPAIVVADIEREKPMTVEWS
TYAQSLTSKQMKGMLTGPVTILCWTFPREDISRKEITNQLAFALRDEVSDLQDAGINIIQIDEPAIREGLPLKKRDHAEY
LEWAVDAFKISAASAKPETQIHTHMCYSEFNEIIDSVAALDADVITIETSRSNMELLKAFEEFNYPNAIGPGVYDIHSPN
IPSEEWIVDLLKKAAEKIPAERLWANPDCGLKTRNWAETEAALTNLVSATKTLRKEWESAEA
>Mature_781_residues
TTTTHILGYPRIGEKRELKFTLEKYWRGEIDQSELKQTGSELRNRNWNVQADANLSFATAGDFAWYDHVLTTTLLLGHVP
KRHAGGSEDEKAFPDLDTLFRVGRGQSQVQSTCCGGSHTSNDVTKDSSAASDMTKWFNTNYHYIVPEFSKDDSFEVSWPQ
LFDEVNEAIKAGHKVKPVLLGPLSYLYLGKEVEDGFDRLSLLPRLLTAYQAILAKLSKLGVEWVQIDEPILALELDNQWV
DSFKLAYQVIQGDVKLLLTTYFDSVTDTLDKIVDLPINGLHIDLAAAPQQLDEVVNKLPEDWVLSAGAINGRNVWRADLA
AQLELLQPVKDKLGDKLWIASSCSLLHSPVDLELEETLSEEVKSWFAFAKQKVTEVSLLGAALDGDQNAILACETYSQPI
VARKSATHVNKPQVQARINTITKALAERSAPYAERAAHQSEVLGLPLLPTTTIGSFPQTGEIRLQRSAYRTGKLNEAEYT
TALKGHIADAVKRQEALDLDVLVHGEAERNDMVEYFAENLAGFQTTKFGWVQSYGSRCVKPAIVVADIEREKPMTVEWST
YAQSLTSKQMKGMLTGPVTILCWTFPREDISRKEITNQLAFALRDEVSDLQDAGINIIQIDEPAIREGLPLKKRDHAEYL
EWAVDAFKISAASAKPETQIHTHMCYSEFNEIIDSVAALDADVITIETSRSNMELLKAFEEFNYPNAIGPGVYDIHSPNI
PSEEWIVDLLKKAAEKIPAERLWANPDCGLKTRNWAETEAALTNLVSATKTLRKEWESAEA

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation [H]

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family [H]

Homologues:

Organism=Escherichia coli, GI2367304, Length=776, Percent_Identity=59.2783505154639, Blast_Score=932, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320936, Length=781, Percent_Identity=42.8937259923175, Blast_Score=652, Evalue=0.0,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629 [H]

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2 [H]

EC number: =2.1.1.14 [H]

Molecular weight: Translated: 87340; Mature: 87209

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTTHILGYPRIGEKRELKFTLEKYWRGEIDQSELKQTGSELRNRNWNVQADANLSFAT
CCCCCEEECCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEECCCEEEEE
AGDFAWYDHVLTTTLLLGHVPKRHAGGSEDEKAFPDLDTLFRVGRGQSQVQSTCCGGSHT
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHCCCCCC
SNDVTKDSSAASDMTKWFNTNYHYIVPEFSKDDSFEVSWPQLFDEVNEAIKAGHKVKPVL
CCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCEECHHHHHHHHHHHHHCCCCCCCEE
LGPLSYLYLGKEVEDGFDRLSLLPRLLTAYQAILAKLSKLGVEWVQIDEPILALELDNQW
ECCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEECCHH
VDSFKLAYQVIQGDVKLLLTTYFDSVTDTLDKIVDLPINGLHIDLAAAPQQLDEVVNKLP
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHCCHHHHHHHHHHCC
EDWVLSAGAINGRNVWRADLAAQLELLQPVKDKLGDKLWIASSCSLLHSPVDLELEETLS
HHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHCCCCCCCHHHHHH
EEVKSWFAFAKQKVTEVSLLGAALDGDQNAILACETYSQPIVARKSATHVNKPQVQARIN
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEECCCCCCCCCCHHHHHHH
TITKALAERSAPYAERAAHQSEVLGLPLLPTTTIGSFPQTGEIRLQRSAYRTGKLNEAEY
HHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEHHHHCCCCCCCHHH
TTALKGHIADAVKRQEALDLDVLVHGEAERNDMVEYFAENLAGFQTTKFGWVQSYGSRCV
HHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
KPAIVVADIEREKPMTVEWSTYAQSLTSKQMKGMLTGPVTILCWTFPREDISRKEITNQL
CCEEEEEECCCCCCCEEEHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHCHHHHHHHHH
AFALRDEVSDLQDAGINIIQIDEPAIREGLPLKKRDHAEYLEWAVDAFKISAASAKPETQ
HHHHHHHHHHHHHCCCEEEEECCHHHHCCCCCCCCHHHHHHHHHHHHHHEECCCCCCCHH
IHTHMCYSEFNEIIDSVAALDADVITIETSRSNMELLKAFEEFNYPNAIGPGVYDIHSPN
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCCEECCCCC
IPSEEWIVDLLKKAAEKIPAERLWANPDCGLKTRNWAETEAALTNLVSATKTLRKEWESA
CCHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
EA
CC
>Mature Secondary Structure 
TTTTHILGYPRIGEKRELKFTLEKYWRGEIDQSELKQTGSELRNRNWNVQADANLSFAT
CCCCEEECCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEECCCEEEEE
AGDFAWYDHVLTTTLLLGHVPKRHAGGSEDEKAFPDLDTLFRVGRGQSQVQSTCCGGSHT
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHCCCCCC
SNDVTKDSSAASDMTKWFNTNYHYIVPEFSKDDSFEVSWPQLFDEVNEAIKAGHKVKPVL
CCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCEECHHHHHHHHHHHHHCCCCCCCEE
LGPLSYLYLGKEVEDGFDRLSLLPRLLTAYQAILAKLSKLGVEWVQIDEPILALELDNQW
ECCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEECCHH
VDSFKLAYQVIQGDVKLLLTTYFDSVTDTLDKIVDLPINGLHIDLAAAPQQLDEVVNKLP
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHCCHHHHHHHHHHCC
EDWVLSAGAINGRNVWRADLAAQLELLQPVKDKLGDKLWIASSCSLLHSPVDLELEETLS
HHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHCCCCCCCHHHHHH
EEVKSWFAFAKQKVTEVSLLGAALDGDQNAILACETYSQPIVARKSATHVNKPQVQARIN
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEECCCCCCCCCCHHHHHHH
TITKALAERSAPYAERAAHQSEVLGLPLLPTTTIGSFPQTGEIRLQRSAYRTGKLNEAEY
HHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEHHHHCCCCCCCHHH
TTALKGHIADAVKRQEALDLDVLVHGEAERNDMVEYFAENLAGFQTTKFGWVQSYGSRCV
HHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
KPAIVVADIEREKPMTVEWSTYAQSLTSKQMKGMLTGPVTILCWTFPREDISRKEITNQL
CCEEEEEECCCCCCCEEEHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHCHHHHHHHHH
AFALRDEVSDLQDAGINIIQIDEPAIREGLPLKKRDHAEYLEWAVDAFKISAASAKPETQ
HHHHHHHHHHHHHCCCEEEEECCHHHHCCCCCCCCHHHHHHHHHHHHHHEECCCCCCCHH
IHTHMCYSEFNEIIDSVAALDADVITIETSRSNMELLKAFEEFNYPNAIGPGVYDIHSPN
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCCEECCCCC
IPSEEWIVDLLKKAAEKIPAERLWANPDCGLKTRNWAETEAALTNLVSATKTLRKEWESA
CCHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
EA
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA